{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T11:30:25Z","timestamp":1791804625684,"version":"4.3.4"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Shandong Provincial Science and Technology Development Project for Medicine and Health","award":["202105031068"],"award-info":[{"award-number":["202105031068"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11033-025-10553-9","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T08:53:24Z","timestamp":1747126404000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Exploring the role of Myo-inositol in alleviating insulin resistance in polycystic ovary syndrome through the AMPK\/GLUT4 pathway"],"prefix":"10.1007","volume":"52","author":[{"given":"Guihong","family":"Song","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nannan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajin","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saisai","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiping","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,5,13]]},"reference":[{"key":"10553_CR1","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1016\/s2213-8587(22)00163-2","volume":"10","author":"AE Joham","year":"2022","unstructured":"Joham AE, Norman RJ, Stener-Victorin E et al (2022) Polycystic ovary syndrome. Lancet Diabetes Endocrinol 10:668\u2013680. https:\/\/doi.org\/10.1016\/s2213-8587(22)00163-2","journal-title":"Lancet Diabetes Endocrinol"},{"key":"10553_CR2","doi-asserted-by":"publisher","first-page":"1291","DOI":"10.4158\/ep15748.dsc","volume":"21","author":"NF Goodman","year":"2015","unstructured":"Goodman NF, Cobin RH, Futterweit W, Glueck JS, Legro RS, Carmina E, American association of clinical endocrinologists, american college of endocrinology, and androgen excess and pcos society disease state clinical review: guide to the best practices in the evaluation and treatment of polycystic ovary syndrome\u2013part 1 (2015) Endocr Pract 21:1291\u20131300. https:\/\/doi.org\/10.4158\/ep15748.dsc","journal-title":"Endocr Pract"},{"key":"10553_CR3","doi-asserted-by":"publisher","first-page":"1454","DOI":"10.3390\/jcm12041454","volume":"12","author":"S Singh","year":"2023","unstructured":"Singh S, Pal N, Shubham S et al (2023) Polycystic ovary syndrome: etiology, current management, and future therapeutics. J Clin Med 12:1454. https:\/\/doi.org\/10.3390\/jcm12041454","journal-title":"J Clin Med"},{"key":"10553_CR4","doi-asserted-by":"publisher","first-page":"1091147","DOI":"10.3389\/fendo.2022.1091147","volume":"13","author":"C Tong","year":"2022","unstructured":"Tong C, Wu Y, Zhang L, Yu Y (2022) Insulin resistance, autophagy and apoptosis in patients with polycystic ovary syndrome: association with PI3K signaling pathway. Front Endocrinol (Lausanne) 13:1091147. https:\/\/doi.org\/10.3389\/fendo.2022.1091147","journal-title":"Front Endocrinol (Lausanne)"},{"key":"10553_CR5","doi-asserted-by":"publisher","first-page":"616","DOI":"10.4239\/wjd.v12.i5.616","volume":"12","author":"K Unluhizarci","year":"2021","unstructured":"Unluhizarci K, Karaca Z, Kelestimur F (2021) Role of insulin and insulin resistance in androgen excess disorders. World J Diabetes 12:616\u2013629. https:\/\/doi.org\/10.4239\/wjd.v12.i5.616","journal-title":"World J Diabetes"},{"key":"10553_CR6","doi-asserted-by":"publisher","first-page":"154458","DOI":"10.1016\/j.metabol.2020.154458","volume":"115","author":"N Chahal","year":"2021","unstructured":"Chahal N, Geethadevi A, Kaur S et al (2021) Direct impact of gonadotropins on glucose uptake and storage in preovulatory granulosa cells: implications in the pathogenesis of polycystic ovary syndrome. Metabolism 115:154458. https:\/\/doi.org\/10.1016\/j.metabol.2020.154458","journal-title":"Metabolism"},{"key":"10553_CR7","doi-asserted-by":"publisher","first-page":"11303","DOI":"10.1096\/fj.201802829R","volume":"33","author":"X Li","year":"2019","unstructured":"Li X, Zhu Q, Wang W et al (2019) Elevated chemerin induces insulin resistance in human granulosa-lutein cells from polycystic ovary syndrome patients. Faseb J 33:11303\u201311313. https:\/\/doi.org\/10.1096\/fj.201802829R","journal-title":"Faseb J"},{"key":"10553_CR8","doi-asserted-by":"publisher","first-page":"113327","DOI":"10.1016\/j.intimp.2024.113327","volume":"143","author":"L Weng","year":"2024","unstructured":"Weng L, Zhu Q, Xiang Y et al (2024) METTL3-mediated m(6)A modification of CD36: implications for glucose metabolism and inflammatory dysregulation in follicles of polycystic ovary syndrome. Int Immunopharmacol 143:113327. https:\/\/doi.org\/10.1016\/j.intimp.2024.113327","journal-title":"Int Immunopharmacol"},{"key":"10553_CR9","doi-asserted-by":"publisher","first-page":"2235","DOI":"10.1093\/humrep\/det116","volume":"28","author":"R Carvajal","year":"2013","unstructured":"Carvajal R, Rosas C, Kohan K et al (2013) Metformin augments the levels of molecules that regulate the expression of the insulin-dependent glucose transporter GLUT4 in the endometria of hyperinsulinemic PCOS patients. Hum Reprod 28:2235\u20132244. https:\/\/doi.org\/10.1093\/humrep\/det116","journal-title":"Hum Reprod"},{"key":"10553_CR10","doi-asserted-by":"publisher","first-page":"104544","DOI":"10.1016\/j.yrtph.2019.104544","volume":"110","author":"N Zhang","year":"2020","unstructured":"Zhang N, Liu X, Zhuang L et al (2020) Berberine decreases insulin resistance in a PCOS rats by improving GLUT4: dual regulation of the PI3K\/AKT and MAPK pathways. Regul Toxicol Pharmacol 110:104544. https:\/\/doi.org\/10.1016\/j.yrtph.2019.104544","journal-title":"Regul Toxicol Pharmacol"},{"key":"10553_CR11","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1038\/s41419-022-04541-1","volume":"13","author":"X Zhang","year":"2022","unstructured":"Zhang X, Zhang W, Wang Z et al (2022) Enhanced Glycolysis in granulosa cells promotes the activation of primordial follicles through mTOR signaling. Cell Death Dis 13:87. https:\/\/doi.org\/10.1038\/s41419-022-04541-1","journal-title":"Cell Death Dis"},{"key":"10553_CR12","doi-asserted-by":"publisher","first-page":"860","DOI":"10.2337\/db07-0843","volume":"57","author":"SL McGee","year":"2008","unstructured":"McGee SL, van Denderen BJ, Howlett KF et al (2008) AMP-activated protein kinase regulates GLUT4 transcription by phosphorylating histone deacetylase 5. Diabetes 57:860\u2013867. https:\/\/doi.org\/10.2337\/db07-0843","journal-title":"Diabetes"},{"key":"10553_CR13","doi-asserted-by":"publisher","first-page":"112563","DOI":"10.1016\/j.biopha.2021.112563","volume":"146","author":"M Entezari","year":"2022","unstructured":"Entezari M, Hashemi D, Taheriazam A et al (2022) AMPK signaling in diabetes mellitus, insulin resistance and diabetic complications: A pre-clinical and clinical investigation. Biomed Pharmacother 146:112563. https:\/\/doi.org\/10.1016\/j.biopha.2021.112563","journal-title":"Biomed Pharmacother"},{"key":"10553_CR14","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1002\/cbf.3330","volume":"36","author":"S Bakhshalizadeh","year":"2018","unstructured":"Bakhshalizadeh S, Amidi F, Shirazi R, Shabani Nashtaei M (2018) Vitamin D3 regulates steroidogenesis in granulosa cells through AMP-activated protein kinase (AMPK) activation in a mouse model of polycystic ovary syndrome. Cell Biochem Funct 36:183\u2013193. https:\/\/doi.org\/10.1002\/cbf.3330","journal-title":"Cell Biochem Funct"},{"key":"10553_CR15","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1210\/endrev\/bnad012","volume":"44","author":"LK Townsend","year":"2023","unstructured":"Townsend LK, Steinberg GR (2023) AMPK and the endocrine control of metabolism. Endocr Rev 44:910\u2013933. https:\/\/doi.org\/10.1210\/endrev\/bnad012","journal-title":"Endocr Rev"},{"key":"10553_CR16","doi-asserted-by":"publisher","first-page":"1868","DOI":"10.1111\/jgh.16272","volume":"38","author":"IA Marcondes-de-Castro","year":"2023","unstructured":"Marcondes-de-Castro IA, Reis-Barbosa PH, Marinho TS, Aguila MB, Mandarim-de-Lacerda CA (2023) AMPK\/mTOR pathway significance in healthy liver and non-alcoholic fatty liver disease and its progression. J Gastroenterol Hepatol 38:1868\u20131876. https:\/\/doi.org\/10.1111\/jgh.16272","journal-title":"J Gastroenterol Hepatol"},{"key":"10553_CR17","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1111\/dom.12854","volume":"19","author":"F Bonnet","year":"2017","unstructured":"Bonnet F, Scheen A (2017) Understanding and overcoming Metformin Gastrointestinal intolerance. Diabetes Obes Metab 19:473\u2013481. https:\/\/doi.org\/10.1111\/dom.12854","journal-title":"Diabetes Obes Metab"},{"key":"10553_CR18","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1530\/ec-17-0243","volume":"6","author":"V Unfer","year":"2017","unstructured":"Unfer V, Facchinetti F, Orr\u00f9 B, Giordani B, Nestler J (2017) Myo-inositol effects in women with PCOS: a meta-analysis of randomized controlled trials. Endocr Connect 6:647\u2013658. https:\/\/doi.org\/10.1530\/ec-17-0243","journal-title":"Endocr Connect"},{"key":"10553_CR19","doi-asserted-by":"publisher","first-page":"7476","DOI":"10.26355\/eurrev_202112_27447","volume":"25","author":"L Pkhaladze","year":"2021","unstructured":"Pkhaladze L, Russo M, Unfer V, Nordio M, Basciani S, Khomasuridze A (2021) Treatment of lean PCOS teenagers: a follow-up comparison between Myo-Inositol and oral contraceptives. Eur Rev Med Pharmacol Sci 25:7476\u20137485. https:\/\/doi.org\/10.26355\/eurrev_202112_27447","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"10553_CR20","doi-asserted-by":"publisher","first-page":"1875","DOI":"10.3390\/nu15081875","volume":"15","author":"M Bizzarri","year":"2023","unstructured":"Bizzarri M, Monti N, Piombarolo A, Angeloni A, Verna R (2023) Myo-Inositol and D-Chiro-Inositol as modulators of ovary steroidogenesis: A narrative review. Nutrients 15:1875. https:\/\/doi.org\/10.3390\/nu15081875","journal-title":"Nutrients"},{"key":"10553_CR21","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1159\/000345310","volume":"78","author":"LS Vilmann","year":"2012","unstructured":"Vilmann LS, Thisted E, Baker JL, Holm JC (2012) Development of obesity and polycystic ovary syndrome in adolescents. Horm Res Paediatr 78:269\u2013278. https:\/\/doi.org\/10.1159\/000345310","journal-title":"Horm Res Paediatr"},{"key":"10553_CR22","doi-asserted-by":"publisher","first-page":"3506","DOI":"10.1097\/ms9.0000000000000879","volume":"85","author":"A Mohan","year":"2023","unstructured":"Mohan A, Haider R, Fakhor H et al (2023) Vitamin D and polycystic ovary syndrome (PCOS): a review. Ann Med Surg (Lond) 85:3506\u20133511. https:\/\/doi.org\/10.1097\/ms9.0000000000000879","journal-title":"Ann Med Surg (Lond)"},{"key":"10553_CR23","doi-asserted-by":"publisher","first-page":"2994","DOI":"10.1038\/s41467-023-38401-z","volume":"14","author":"JM Park","year":"2023","unstructured":"Park JM, Lee DH, Kim DH (2023) Redefining the role of AMPK in autophagy and the energy stress response. Nat Commun 14:2994. https:\/\/doi.org\/10.1038\/s41467-023-38401-z","journal-title":"Nat Commun"},{"key":"10553_CR24","doi-asserted-by":"publisher","first-page":"4987","DOI":"10.3390\/ijms21144987","volume":"21","author":"S Wu","year":"2020","unstructured":"Wu S, Zou MH (2020) AMPK, mitochondrial function, and cardiovascular disease. Int J Mol Sci 21:4987. https:\/\/doi.org\/10.3390\/ijms21144987","journal-title":"Int J Mol Sci"},{"key":"10553_CR25","doi-asserted-by":"publisher","first-page":"16247","DOI":"10.3390\/ijms242216247","volume":"24","author":"CH Zhang","year":"2023","unstructured":"Zhang CH, Liu XY, Wang J (2023) Essential role of granulosa cell glucose and lipid metabolism on oocytes and the potential metabolic imbalance in polycystic ovary syndrome. Int J Mol Sci 24:16247. https:\/\/doi.org\/10.3390\/ijms242216247","journal-title":"Int J Mol Sci"},{"key":"10553_CR26","doi-asserted-by":"publisher","first-page":"3337","DOI":"10.2147\/dddt.s468147","volume":"18","author":"RY Elkanawati","year":"2024","unstructured":"Elkanawati RY, Sumiwi SA, Levita J (2024) Impact of lipids on insulin resistance: insights from human and animal studies. Drug Des Devel Ther 18:3337\u20133360. https:\/\/doi.org\/10.2147\/dddt.s468147","journal-title":"Drug Des Devel Ther"},{"key":"10553_CR27","doi-asserted-by":"publisher","first-page":"153739","DOI":"10.1016\/j.phymed.2021.153739","volume":"92","author":"R Huang","year":"2021","unstructured":"Huang R, Guo F, Li Y et al (2021) Activation of AMPK by triptolide alleviates nonalcoholic fatty liver disease by improving hepatic lipid metabolism, inflammation and fibrosis. Phytomedicine 92:153739. https:\/\/doi.org\/10.1016\/j.phymed.2021.153739","journal-title":"Phytomedicine"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-10553-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-025-10553-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-10553-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T16:33:41Z","timestamp":1766853221000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-025-10553-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,13]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10553"],"URL":"https:\/\/doi.org\/10.1007\/s11033-025-10553-9","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,13]]},"assertion":[{"value":"19 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 April 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 May 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The Animal Ethics Committee of Shandong College of Traditional Chinese Medicine approved the experimental procedures under protocol 2021\u2009\u2212\u20091068. All experiments were conducted following national and institutional guidelines for the care and use of laboratory animals.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"454"}}