{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T06:00:58Z","timestamp":1766901658973,"version":"3.48.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T00:00:00Z","timestamp":1752624000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T00:00:00Z","timestamp":1752624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100005851","name":"Shahid Beheshti University of Medical Sciences","doi-asserted-by":"publisher","award":["RIGLD- 1059"],"award-info":[{"award-number":["RIGLD- 1059"]}],"id":[{"id":"10.13039\/501100005851","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11033-025-10823-6","type":"journal-article","created":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T13:36:01Z","timestamp":1752672961000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Toxoplasma gondii soluble antigen (STAg) induces resistance against Mycobacterium tuberculosis in a human monocyte cell line via P2X7 receptor"],"prefix":"10.1007","volume":"52","author":[{"given":"Parisa","family":"Ilbeigi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javid","family":"Sadraei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh","family":"Ghaffarifar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed Davar","family":"Siadat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2568-9022","authenticated-orcid":false,"given":"Hamed","family":"Mirjalali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,16]]},"reference":[{"issue":"14","key":"10823_CR1","doi-asserted-by":"publisher","first-page":"R770","DOI":"10.1016\/j.cub.2018.05.035","volume":"28","author":"JA Kochanowsky","year":"2018","unstructured":"Kochanowsky JA, Koshy AA (2018) Toxoplasma gondii. Curr Biol: CB 28(14):R770\u2013r771","journal-title":"Curr Biol: CB"},{"issue":"9425","key":"10823_CR2","doi-asserted-by":"publisher","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","volume":"363","author":"JG Montoya","year":"2004","unstructured":"Montoya JG, Liesenfeld O (2004) Toxoplasmosis. Lancet (London England) 363(9425):1965\u20131976","journal-title":"Lancet (London England)"},{"key":"10823_CR3","doi-asserted-by":"crossref","unstructured":"Holec-G\u0105sior L, So\u0142owi\u0144ska K (2023) Detection of Toxoplasma gondii infection in small ruminants: old problems, and current solutions. Animals: an open access journal from MDPI 13(17)","DOI":"10.3390\/ani13172696"},{"key":"10823_CR4","doi-asserted-by":"crossref","unstructured":"Daher D, Shaghlil A, Sobh E, Hamie M, Hassan ME, Moumneh MB, Itani S, El Hajj R, Tawk L, El Sabban M et al (2021) Comprehensive overview of Toxoplasma gondii-Induced and associated diseases. Pathogens (Basel Switzerland) 10(11)","DOI":"10.3390\/pathogens10111351"},{"key":"10823_CR5","doi-asserted-by":"publisher","first-page":"102226","DOI":"10.1016\/j.coi.2022.102226","volume":"77","author":"IA Khan","year":"2022","unstructured":"Khan IA, Moretto M (2022) Immune responses to Toxoplasma gondii. Curr Opin Immunol 77:102226","journal-title":"Curr Opin Immunol"},{"key":"10823_CR6","doi-asserted-by":"publisher","first-page":"103","DOI":"10.3389\/fcimb.2019.00103","volume":"9","author":"TS Lima","year":"2019","unstructured":"Lima TS, Lodoen MB (2019) Mechanisms of human innate immune evasion by Toxoplasma gondii. Front Cell Infect Microbiol 9:103","journal-title":"Front Cell Infect Microbiol"},{"issue":"7","key":"10823_CR7","doi-asserted-by":"publisher","first-page":"e12712","DOI":"10.1111\/pim.12712","volume":"42","author":"J Park","year":"2020","unstructured":"Park J, Hunter CA (2020) The role of macrophages in protective and pathological responses to Toxoplasma gondii. Parasite Immunol 42(7):e12712","journal-title":"Parasite Immunol"},{"issue":"1","key":"10823_CR8","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/s12866-023-02819-8","volume":"23","author":"S Nemati","year":"2023","unstructured":"Nemati S, Mohammad Rahimi H, Meyfour A, Pazoki H, Asadzadeh Aghdaei H, Shahrokh S, Mirjalali H (2023) Evaluation of the mTORC activity in the presence of Toxoplasma gondii and azathioprine in human monocyte cell line. BMC Microbiol 23(1):77","journal-title":"BMC Microbiol"},{"issue":"10","key":"10823_CR9","doi-asserted-by":"publisher","first-page":"7041","DOI":"10.1007\/s11033-021-06667-5","volume":"48","author":"S Nemati","year":"2021","unstructured":"Nemati S, Pazoki H, Mohammad Rahimi H, Asadzadeh Aghdaei H, Shahrokh S, Baghaei K, Mirjalali H, Zali MR (2021)Toxoplasma gondii profilin and tachyzoites RH strain May manipulate autophagy via downregulating Atg5 and Atg12 and upregulating Atg7. Mol Biol Rep 48(10):7041\u20137047","journal-title":"Mol Biol Rep"},{"issue":"1","key":"10823_CR10","doi-asserted-by":"publisher","first-page":"e0259523","DOI":"10.1128\/mbio.02595-23","volume":"15","author":"N Rinkenberger","year":"2024","unstructured":"Rinkenberger N, Rosenberg A, Radke JB, Bhushan J, Tomita T, Weiss LM, Sibley LD (2024) Susceptibility of Toxoplasma gondii to autophagy in human cells relies on multiple interacting parasite loci. mBio 15(1):e0259523","journal-title":"mBio"},{"key":"10823_CR11","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1007\/5584_2017_59","volume":"1051","author":"R Sluyter","year":"2017","unstructured":"Sluyter R (2017) The P2X7 Receptor. Adv Exp Med Biol 1051:17\u201353","journal-title":"Adv Exp Med Biol"},{"key":"10823_CR12","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/bs.mie.2019.07.043","volume":"629","author":"F Di Virgilio","year":"2019","unstructured":"Di Virgilio F, Jiang LH, Roger S, Falzoni S, Sarti AC, Vultaggio-Poma V, Chiozzi P, Adinolfi E (2019) Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cells. Methods Enzymol 629:115\u2013150","journal-title":"Methods Enzymol"},{"issue":"5","key":"10823_CR13","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1111\/j.1399-0039.2011.01780.x","volume":"78","author":"JS Wiley","year":"2011","unstructured":"Wiley JS, Sluyter R, Gu BJ, Stokes L, Fuller SJ (2011) The human P2X7 receptor and its role in innate immunity. Tissue Antigens 78(5):321\u2013332","journal-title":"Tissue Antigens"},{"issue":"1","key":"10823_CR14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13071-017-2573-y","volume":"11","author":"JH Quan","year":"2018","unstructured":"Quan JH, Huang R, Wang Z, Huang S, Choi IW, Zhou Y, Lee YH, Chu JQ (2018) P2X7 receptor mediates NLRP3-dependent IL-1\u03b2 secretion and parasite proliferation in Toxoplasma gondii-infected human small intestinal epithelial cells. Parasit Vectors 11(1):1","journal-title":"Parasit Vectors"},{"key":"10823_CR15","doi-asserted-by":"publisher","first-page":"1257","DOI":"10.3389\/fimmu.2017.01257","volume":"8","author":"ACA Moreira-Souza","year":"2017","unstructured":"Moreira-Souza ACA, Almeida-da-Silva CLC, Rangel TP, Rocha GDC, Bellio M, Zamboni DS, Vommaro RC, Coutinho-Silva R (2017) The P2X7 receptor mediates Toxoplasma gondii control in macrophages through canonical NLRP3 inflammasome activation and reactive oxygen species production. Front Immunol 8:1257","journal-title":"Front Immunol"},{"issue":"7","key":"10823_CR16","doi-asserted-by":"publisher","first-page":"e1004188","DOI":"10.1371\/journal.ppat.1004188","volume":"10","author":"EP Amaral","year":"2014","unstructured":"Amaral EP, Ribeiro SC, Lanes VR, Almeida FM, de Andrade MR, Bomfim CC, Salles EM, Bortoluci KR, Coutinho-Silva R, Hirata MH et al (2014) Pulmonary infection with hypervirulent Mycobacteria reveals a crucial role for the P2X7 receptor in aggressive forms of tuberculosis. PLoS Pathog 10(7):e1004188","journal-title":"PLoS Pathog"},{"key":"10823_CR17","doi-asserted-by":"crossref","unstructured":"Rahimi HM, Nemati S, Alavifard H, Baghaei K, Mirjalali H, Zali MR (2021) Soluble total antigen derived from Toxoplasma gondii RH strain prevents apoptosis, but induces anti-apoptosis in human monocyte cell line. Folia Parasitol 68","DOI":"10.14411\/fp.2021.026"},{"key":"10823_CR18","doi-asserted-by":"publisher","first-page":"106120","DOI":"10.1016\/j.micpath.2023.106120","volume":"180","author":"H Pazoki","year":"2023","unstructured":"Pazoki H, Mirjalali H, Niyyati M, Seyed Tabaei SJ, Mosaffa N, Shahrokh S, Asadzadeh Ahdaei H, Kupz A, Zali MR (2023)Toxoplasma gondii profilin induces NLRP3 activation and IL-1\u03b2 production\/secretion in THP-1 cells. Microb Pathog 180:106120","journal-title":"Microb Pathog"},{"issue":"4","key":"10823_CR19","first-page":"1243","volume":"44","author":"YW Zhang","year":"2019","unstructured":"Zhang YW, Lin Y, Yu HY, Tian RN, Li F (2019) Characteristic genes in THP\u20131 derived macrophages infected with Mycobacterium tuberculosis H37Rv strain identified by integrating bioinformatics methods. Int J Mol Med 44(4):1243\u20131254","journal-title":"Int J Mol Med"},{"issue":"1","key":"10823_CR20","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1128\/IAI.71.1.254-259.2003","volume":"71","author":"CJ Riendeau","year":"2003","unstructured":"Riendeau CJ, Kornfeld H (2003) THP-1 cell apoptosis in response to mycobacterial infection. Infect Immun 71(1):254\u2013259","journal-title":"Infect Immun"},{"issue":"3","key":"10823_CR21","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1111\/ocr.12307","volume":"22","author":"J Shi","year":"2019","unstructured":"Shi J, Folwaczny M, Wichelhaus A, Baumert U (2019) Differences in RUNX2 and P2RX7 gene expression between mono- and coculture of human periodontal ligament cells and human osteoblasts under compressive force application. Orthod Craniofac Res 22(3):168\u2013176","journal-title":"Orthod Craniofac Res"},{"issue":"6","key":"10823_CR22","doi-asserted-by":"publisher","first-page":"e343","DOI":"10.1038\/emm.2017.69","volume":"49","author":"J Li","year":"2017","unstructured":"Li J, Wang P, Xie Z, Yang R, Li Y, Wu X, Su H, Deng W, Wang S, Liu Z et al (2017) Elevated TRAF4 expression impaired LPS-induced autophagy in mesenchymal stem cells from ankylosing spondylitis patients. Exp Mol Med 49(6):e343","journal-title":"Exp Mol Med"},{"key":"10823_CR23","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.jgar.2019.01.003","volume":"17","author":"J Kardan-Yamchi","year":"2019","unstructured":"Kardan-Yamchi J, Kazemian H, Haeili M, Harati AA, Amini S, Feizabadi MM (2019) Expression analysis of 10 efflux pump genes in multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis clinical isolates. J Glob Antimicrob Resist 17:201\u2013208","journal-title":"J Glob Antimicrob Resist"},{"key":"10823_CR24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.phymed.2018.09.233","volume":"53","author":"TL Huang","year":"2019","unstructured":"Huang TL, Yang SH, Chen YR, Liao JY, Tang Y, Yang KC (2019) The therapeutic effect of aucubin-supplemented hyaluronic acid on interleukin-1beta-stimulated human articular chondrocytes. Phytomedicine 53:1\u20138","journal-title":"Phytomedicine"},{"key":"10823_CR25","doi-asserted-by":"publisher","first-page":"347","DOI":"10.3389\/fcimb.2017.00347","volume":"7","author":"L Petit-Jentreau","year":"2017","unstructured":"Petit-Jentreau L, Tailleux L, Coombes JL (2017) Purinergic signaling: A common path in the macrophage response against Mycobacterium tuberculosis and Toxoplasma gondii. Front Cell Infect Microbiol 7:347","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"10823_CR26","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1093\/infdis\/123.1.22","volume":"123","author":"LO Gentry","year":"1971","unstructured":"Gentry LO, Remington JS (1971) Resistance against Cryptococcus conferred by intracellular bacteria and protozoa. J Infect Dis 123(1):22\u201331","journal-title":"J Infect Dis"},{"issue":"3823","key":"10823_CR27","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1126\/science.160.3823.72","volume":"160","author":"J Ruskin","year":"1968","unstructured":"Ruskin J, Remington JS (1968) Immunity and intracellular infection: resistance to bacteria in mice infected with a protozoan. Science 160(3823):72\u201374","journal-title":"Science"},{"issue":"6","key":"10823_CR28","doi-asserted-by":"publisher","first-page":"e1004203","DOI":"10.1371\/journal.ppat.1004203","volume":"10","author":"LM Neal","year":"2014","unstructured":"Neal LM, Knoll LJ (2014)Toxoplasma gondii profilin promotes recruitment of Ly6Chi CCR2\u2009+\u2009inflammatory monocytes that can confer resistance to bacterial infection. PLoS Pathog 10(6):e1004203","journal-title":"PLoS Pathog"},{"issue":"5572","key":"10823_CR29","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1038\/263056a0","volume":"263","author":"AA Mahmoud","year":"1976","unstructured":"Mahmoud AA, Warren KS, Strickland GT (1976) Acquired resistance to infection with Schistosoma mansoni induced by Toxoplasma gondii. Nature 263(5572):56\u201357","journal-title":"Nature"},{"issue":"4","key":"10823_CR30","doi-asserted-by":"publisher","first-page":"1184","DOI":"10.3181\/00379727-131-34066","volume":"131","author":"JS Remington","year":"1969","unstructured":"Remington JS, Merigan TC (1969) Resistance to virus challenge in mice infected with protozoa or bacteria. Proc Soc Exp Biol Med 131(4):1184\u20131188","journal-title":"Proc Soc Exp Biol Med"},{"issue":"1","key":"10823_CR31","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/0008-8749(77)90238-6","volume":"34","author":"R McLeod","year":"1977","unstructured":"McLeod R, Remington JS (1977) Studies on the specificity of killing of intracellular pathogens by macrophages. Cell Immunol 34(1):156\u2013174","journal-title":"Cell Immunol"},{"issue":"6","key":"10823_CR32","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1016\/0035-9203(85)90116-6","volume":"79","author":"R McLeod","year":"1985","unstructured":"McLeod R, Estes RG, Cohen H (1985) Influence of Toxoplasma on manifestations of Moloney virus infections. Trans R Soc Trop Med Hyg 79(6):781\u2013787","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"17","key":"10823_CR33","doi-asserted-by":"publisher","first-page":"8680","DOI":"10.1128\/JVI.05142-11","volume":"85","author":"KB O\u2019Brien","year":"2011","unstructured":"O\u2019Brien KB, Schultz-Cherry S, Knoll LJ (2011) Parasite-mediated upregulation of NK cell-derived gamma interferon protects against severe highly pathogenic H5N1 influenza virus infection. J Virol 85(17):8680\u20138688","journal-title":"J Virol"},{"issue":"4","key":"10823_CR34","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1016\/j.ajpath.2019.01.001","volume":"189","author":"ACA Moreira-Souza","year":"2019","unstructured":"Moreira-Souza ACA, Rangel TP, Silva S, Figliuolo VR, Savio LEB, Schmitz F, Takiya CM, Wyse ATS, Vommaro RC, Coutinho-Silva R (2019) Disruption of purinergic receptor P2X7 signaling increases susceptibility to cerebral toxoplasmosis. Am J Pathol 189(4):730\u2013738","journal-title":"Am J Pathol"},{"issue":"6","key":"10823_CR35","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.micinf.2010.03.004","volume":"12","author":"G Corr\u00eaa","year":"2010","unstructured":"Corr\u00eaa G, Marques da Silva C, de Abreu Moreira-Souza AC, Vommaro RC, Coutinho-Silva R (2010) Activation of the P2X(7) receptor triggers the elimination of Toxoplasma gondii tachyzoites from infected macrophages. Microbes Infect 12(6):497\u2013504","journal-title":"Microbes Infect"},{"issue":"1","key":"10823_CR36","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.ijtb.2021.04.004","volume":"69","author":"M Keikha","year":"2022","unstructured":"Keikha M, Karbalaei M (2022) P2X7 polymorphism (rs3751143) and its reliability as a diagnostic biomarker for tuberculosis: A systematic review and meta-analysis. Indian J Tuberc 69(1):85\u201389","journal-title":"Indian J Tuberc"},{"key":"10823_CR37","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/j.meegid.2017.11.020","volume":"57","author":"Y Zhou","year":"2018","unstructured":"Zhou Y, Tan CY, Mo ZJ, Gao QL, He D, Li J, Huang RF, Li YB, Guo CF, Guo Q et al (2018) P2X7 receptor in spinal tuberculosis: Gene polymorphisms and protein levels in Chinese Han population. Infect Genet Evol 57:138\u2013144","journal-title":"Infect Genet Evol"},{"issue":"10","key":"10823_CR38","doi-asserted-by":"publisher","first-page":"5442","DOI":"10.4049\/jimmunol.171.10.5442","volume":"171","author":"BM Saunders","year":"2003","unstructured":"Saunders BM, Fernando SL, Sluyter R, Britton WJ, Wiley JS (2003) A loss-of-function polymorphism in the human P2X7 receptor abolishes ATP-mediated killing of mycobacteria. J Immunol 171(10):5442\u20135446","journal-title":"J Immunol"},{"issue":"8","key":"10823_CR39","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.1016\/j.imbio.2013.03.003","volume":"218","author":"AA Santos Jr","year":"2013","unstructured":"Santos AA Jr., Rodrigues-Junior V, Zanin RF, Borges TJ, Bonorino C, Coutinho-Silva R, Takyia CM, Santos DS, Campos MM, Morrone FB (2013) Implication of purinergic P2X7 receptor in M. tuberculosis infection and host interaction mechanisms: a mouse model study. Immunobiology 218(8):1104\u20131112","journal-title":"Immunobiology"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-10823-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-025-10823-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-10823-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T04:26:37Z","timestamp":1766895997000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-025-10823-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,16]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10823"],"URL":"https:\/\/doi.org\/10.1007\/s11033-025-10823-6","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"type":"print","value":"0301-4851"},{"type":"electronic","value":"1573-4978"}],"subject":[],"published":{"date-parts":[[2025,7,16]]},"assertion":[{"value":"11 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This project was approved by the Ethical Review Committee of the Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran (IR.SBMU.RIGLD.REC.1398.035). In addition, all procedures were reviewed and approved by Tarbiat Modares University, Tehran, Iran (IR.MODARES.REC.1398.102).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"728"}}