{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T06:00:56Z","timestamp":1766901656086,"version":"3.48.0"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11033-025-10826-3","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T13:07:05Z","timestamp":1752844025000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["1-(1-naphthylmethyl)-piperazine Inhibition assay successfully rules out efflux pump overexpression in Acinetobacter baumannii"],"prefix":"10.1007","volume":"52","author":[{"given":"Victor","family":"Zosim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Mullie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Peltier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana\u00efs","family":"Potron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morgane","family":"Choquet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,18]]},"reference":[{"key":"10826_CR1","doi-asserted-by":"publisher","unstructured":"Murray CJL, Ikuta KS, Sharara F, Swetschinski L, Aguilar GR, Gray A et al Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet 2022 12;399:629\u2013655. https:\/\/doi.org\/10.1016\/S0140-6736(21)02724-0","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"10826_CR2","doi-asserted-by":"publisher","unstructured":"Rice LB Federal funding for the study of antimicrobial resistance in nosocomial pathogens: no ESKAPE. J INFECT DIS 2008 15;197:1079\u20131081. https:\/\/doi.org\/10.1086\/533452","DOI":"10.1086\/533452"},{"key":"10826_CR3","unstructured":"WHO TEAM, Antimicrobial Resistance Division, Global Coordination and Partnership, Selection M, IP and, Affordability (2017) Prioritization of pathogens to guide discovery, research and development of new antibiotics for drug-resistant bacterial infections, including tuberculosis [Internet]. World Health Organization (WHO); doi: https:\/\/www.who.int\/publications\/i\/item\/WHO-EMP-IAU-2017.12"},{"key":"10826_CR4","doi-asserted-by":"publisher","unstructured":"Centers for Disease Control and Prevention (U.S.) Antibiotic resistance threats in the United States, 2019 [Internet]. Centers for Disease Control and Prevention (U.S.); 2019 [cited 2024 15]. doi: https:\/\/stacks.cdc.gov\/view\/cdc\/82532doi: https:\/\/doi.org\/10.15620\/cdc:82532","DOI":"10.15620\/cdc:82532"},{"key":"10826_CR5","doi-asserted-by":"publisher","unstructured":"Castanheira M, Mendes RE, Gales AC Global epidemiology and mechanisms of resistance of Acinetobacter baumannii-calcoaceticus complex. Clin Infect Dis 2023 1;76:S166\u2013S178. https:\/\/doi.org\/10.1093\/cid\/ciad109","DOI":"10.1093\/cid\/ciad109"},{"key":"10826_CR6","doi-asserted-by":"publisher","first-page":"1061","DOI":"10.1002\/iub.532","volume":"63","author":"L Poirel","year":"2011","unstructured":"Poirel L, Bonnin RA, Nordmann P (2011) Genetic basis of antibiotic resistance in pathogenic Acinetobacter species. IUBMB Life 63:1061\u20131067. https:\/\/doi.org\/10.1002\/iub.532","journal-title":"IUBMB Life"},{"key":"10826_CR7","doi-asserted-by":"publisher","first-page":"e00514","DOI":"10.1128\/AAC.00514-21","volume":"17","author":"V Kornelsen","year":"2021","unstructured":"Kornelsen V, Kumar A (2021) Update on multidrug resistance efflux pumps in Acinetobacter spp. Antimicrob Agents Chemother 17:65:e00514\u2013e00521. https:\/\/doi.org\/10.1128\/AAC.00514-21","journal-title":"Antimicrob Agents Chemother"},{"key":"10826_CR8","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1038\/s41579-018-0048-6","volume":"16","author":"D Du","year":"2018","unstructured":"Du D, Wang-Kan X, Neuberger A, van Veen HW, Pos KM, Piddock LJV et al (2018) Multidrug efflux pumps: structure, function and regulation. Nat Rev Microbiol 16:523\u2013539. https:\/\/doi.org\/10.1038\/s41579-018-0048-6","journal-title":"Nat Rev Microbiol"},{"key":"10826_CR9","doi-asserted-by":"publisher","first-page":"1919","DOI":"10.1093\/jac\/dkq195","volume":"65","author":"G Rajamohan","year":"2010","unstructured":"Rajamohan G, Srinivasan VB, Gebreyes WA (2010) Molecular and functional characterization of a novel efflux pump, amva, mediating antimicrobial and disinfectant resistance in Acinetobacter baumannii. J Antimicrob Chemother 65:1919\u20131925. https:\/\/doi.org\/10.1093\/jac\/dkq195","journal-title":"J Antimicrob Chemother"},{"key":"10826_CR10","doi-asserted-by":"crossref","unstructured":"Xz L P P, H N. The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria. Clinical microbiology reviews [Internet] 2015 [cited 2023 27];28. doi: https:\/\/pubmed.ncbi.nlm.nih.gov\/25788514\/doi: https:\/\/doi.org\/10.1128\/CMR.00117\u201314","DOI":"10.1128\/CMR.00117-14"},{"key":"10826_CR11","doi-asserted-by":"publisher","unstructured":"Pannek S, Higgins PG, Steinke P, Jonas D, Akova M, Bohnert JA et al Multidrug efflux Inhibition in Acinetobacter baumannii: comparison between 1-(1-naphthylmethyl)-piperazine and phenyl-arginine-\u03b2-naphthylamide. J Antimicrob Chemother 2006 1;57:970\u2013974. https:\/\/doi.org\/10.1093\/jac\/dkl081","DOI":"10.1093\/jac\/dkl081"},{"key":"10826_CR12","doi-asserted-by":"publisher","unstructured":"Choquet M, Lohou E, Pair E, Sonnet P, Mulli\u00e9 C Efflux pump overexpression profiling in Acinetobacter baumannii and study of new 1-(1-Naphthylmethyl)-Piperazine analogs as potential efflux inhibitors. Antimicrob Agents Chemother 2021 17;65:e00710\u2013e00721. https:\/\/doi.org\/10.1128\/AAC.00710-21","DOI":"10.1128\/AAC.00710-21"},{"key":"10826_CR13","doi-asserted-by":"publisher","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","volume":"129","author":"MW Pfaffl","year":"2001","unstructured":"Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT\u2013PCR. Nucleic Acids Res 129:e45","journal-title":"Nucleic Acids Res"},{"key":"10826_CR14","doi-asserted-by":"publisher","first-page":"4174","DOI":"10.1128\/AAC.49.10.4174-4179.2005","volume":"49","author":"C H\u00e9ritier","year":"2005","unstructured":"H\u00e9ritier C, Poirel L, Fournier P-E, Claverie J-M, Raoult D, Nordmann P (2005) Characterization of the naturally occurring oxacillinase of Acinetobacter baumannii. Antimicrob Agents Chemother 49:4174\u20134179. https:\/\/doi.org\/10.1128\/AAC.49.10.4174-4179.2005","journal-title":"Antimicrob Agents Chemother"},{"key":"10826_CR15","doi-asserted-by":"publisher","first-page":"4382","DOI":"10.1128\/JCM.43.9.4382-4390.2005","volume":"43","author":"SG Bartual","year":"2005","unstructured":"Bartual SG, Seifert H, Hippler C, Luzon MAD, Wisplinghoff H, Rodr\u00edguez-Valera F (2005) Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii. J Clin Microbiol 43:4382\u20134390. https:\/\/doi.org\/10.1128\/JCM.43.9.4382-4390.2005","journal-title":"J Clin Microbiol"},{"key":"10826_CR16","doi-asserted-by":"publisher","first-page":"276","DOI":"10.11613\/BM.2012.031","volume":"22","author":"ML McHugh","year":"2012","unstructured":"McHugh ML (2012) Interrater reliability: the kappa statistic. Biochem Med (Zagreb) 22:276\u2013282","journal-title":"Biochem Med (Zagreb)"},{"key":"10826_CR17","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1128\/AAC.45.1.105-116.2001","volume":"45","author":"O Lomovskaya","year":"2001","unstructured":"Lomovskaya O, Warren MS, Lee A, Galazzo J, Fronko R, Lee M et al (2001) Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy. Antimicrob Agents Chemother 45:105\u2013116. https:\/\/doi.org\/10.1128\/AAC.45.1.105-116.2001","journal-title":"Antimicrob Agents Chemother"},{"key":"10826_CR18","doi-asserted-by":"publisher","unstructured":"Bina XR, Philippart JA, Bina JE Effect of the efflux inhibitors 1-(1-naphthylmethyl)-piperazine and phenyl-arginine-\u03b2-naphthylamide on antimicrobial susceptibility and virulence factor production in Vibrio cholerae. J Antimicrob Chemother 2009 1;63:103\u2013108. https:\/\/doi.org\/10.1093\/jac\/dkn466","DOI":"10.1093\/jac\/dkn466"},{"key":"10826_CR19","doi-asserted-by":"publisher","unstructured":"Ribera A, Ruiz J, Jiminez de Anta MT, Vila J Effect of an efflux pump inhibitor on the MIC of Nalidixic acid for Acinetobacter baumannii and Stenotrophomonas maltophilia clinical isolates. J Antimicrob Chemother 2002 1;49:697\u2013698. https:\/\/doi.org\/10.1093\/jac\/49.4.697","DOI":"10.1093\/jac\/49.4.697"},{"key":"10826_CR20","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1128\/aac.48.3.1043-1046.2004","volume":"48","author":"J Chevalier","year":"2004","unstructured":"Chevalier J, Bredin J, Mahamoud A, Mall\u00e9a M, Barbe J, Pag\u00e8s J-M (2004) Inhibitors of antibiotic efflux in resistant Enterobacter aerogenes and Klebsiella pneumoniae strains. Antimicrob Agents Chemother 48:1043\u20131046. https:\/\/doi.org\/10.1128\/aac.48.3.1043-1046.2004","journal-title":"Antimicrob Agents Chemother"},{"key":"10826_CR21","doi-asserted-by":"publisher","first-page":"3511","DOI":"10.1073\/pnas.1419939112","volume":"17","author":"JMA Blair","year":"2015","unstructured":"Blair JMA, Bavro VN, Ricci V, Modi N, Cacciotto P, Kleinekath\u04e7fer U et al (2015) AcrB drug-binding pocket substitution confers clinically relevant resistance and altered substrate specificity. Proc Natl Acad Sci U S A 17:112:3511\u20133516. https:\/\/doi.org\/10.1073\/pnas.1419939112","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10826_CR22","doi-asserted-by":"publisher","unstructured":"Leus IV, Roberts SR, Trinh A, Yu W, Zgurskaya E HI. Nonadditive functional interactions between ligand-binding sites of the multidrug efflux pump AdeB from Acinetobacter baumannii. J Bacteriol 2023 18;206:e00217\u2013e00223. https:\/\/doi.org\/10.1128\/jb.00217-23","DOI":"10.1128\/jb.00217-23"},{"key":"10826_CR23","doi-asserted-by":"publisher","first-page":"2989","DOI":"10.1128\/aac.02556-12","volume":"1157","author":"E-J Yoon","year":"2013","unstructured":"Yoon E-J, Courvalin P, Grillot-Courvalin C (2013) RND-Type efflux pumps in Multidrug-Resistant clinical isolates of Acinetobacter baumannii: major role for AdeABC overexpression and aders mutations. Antimicrob Agents Chemother 1157:2989\u20132995. https:\/\/doi.org\/10.1128\/aac.02556-12","journal-title":"Antimicrob Agents Chemother"},{"key":"10826_CR24","doi-asserted-by":"publisher","first-page":"4389","DOI":"10.1128\/aac.00155-10","volume":"54","author":"S Coyne","year":"2010","unstructured":"Coyne S, Rosenfeld N, Lambert T, Courvalin P, P\u00e9richon B (2010) Overexpression of resistance-Nodulation-Cell division pump AdeFGH confers multidrug resistance in Acinetobacter baumannii. Antimicrob Agents Chemother 54:4389\u20134393. https:\/\/doi.org\/10.1128\/aac.00155-10","journal-title":"Antimicrob Agents Chemother"},{"key":"10826_CR25","doi-asserted-by":"publisher","first-page":"1797","DOI":"10.1128\/aac.48.5.1797-1802.2004","volume":"48","author":"C Llanes","year":"2004","unstructured":"Llanes C, Hocquet D, Vogne C, Benali-Baitich D, Neuwirth C, Pl\u00e9siat P (2004) Clinical strains of Pseudomonas aeruginosa overproducing MexAB-OprM and MexXY efflux pumps simultaneously. Antimicrob Agents Chemother 48:1797\u20131802. https:\/\/doi.org\/10.1128\/aac.48.5.1797-1802.2004","journal-title":"Antimicrob Agents Chemother"},{"key":"10826_CR26","doi-asserted-by":"publisher","unstructured":"Seupt A, Schniederjans M, Tomasch J, H\u00e4ussler S Expression of the MexXY aminoglycoside efflux pump and presence of an aminoglycoside-Modifying enzyme in clinical Pseudomonas aeruginosa isolates are highly correlated. Antimicrob Agents Chemother 2020 16;65:e01166\u2013e01120. https:\/\/doi.org\/10.1128\/AAC.01166-20","DOI":"10.1128\/AAC.01166-20"},{"key":"10826_CR27","doi-asserted-by":"publisher","first-page":"2504","DOI":"10.1128\/aac.06422-11","volume":"1256","author":"N Rosenfeld","year":"2012","unstructured":"Rosenfeld N, Bouchier C, Courvalin P, P\u00e9richon B (2012) Expression of the Resistance-Nodulation-Cell division pump AdeIJK in Acinetobacter baumannii is regulated by aden, a TetR-Type regulator. Antimicrob Agents Chemother 1256:2504\u20132510. https:\/\/doi.org\/10.1128\/aac.06422-11","journal-title":"Antimicrob Agents Chemother"},{"key":"10826_CR28","doi-asserted-by":"publisher","unstructured":"Pournaras S, Gogou V, Giannouli M, Dimitroulia E, Dafopoulou K, Tsakris A et al Single-Locus-Sequence-Based typing of Bla OXA-51-like genes for rapid assignment of Acinetobacter baumannii clinical isolates to international clonal lineages. J Clin Microbiol 2020 21;52:1653\u20131657. https:\/\/doi.org\/10.1128\/jcm.03565-13","DOI":"10.1128\/jcm.03565-13"},{"key":"10826_CR29","doi-asserted-by":"publisher","unstructured":"Gaiarsa S, Batisti Biffignandi G, Esposito EP, Castelli M, Jolley KA, Brisse S et al Comparative Analysis of the Two Acinetobacter baumannii Multilocus Sequence Typing (MLST) Schemes. Front. Microbiol. [Internet] 2019 3 [cited 2024 3];10. doi: https:\/\/www.frontiersin.org\/journals\/microbiology\/articles\/10.3389\/fmicbhttps:\/\/doi.org\/10.3389\/fmicb.2019.00930","DOI":"10.3389\/fmicb.2019.00930"},{"key":"10826_CR30","doi-asserted-by":"publisher","unstructured":"Tomaschek F, Higgins PG, Stefanik D, Wisplinghoff H, Seifert H Head-to-Head comparison of two Multi-Locus sequence typing (MLST) schemes for characterization of Acinetobacter baumannii outbreak and sporadic isolates. PLoS ONE 2016 12;11:e0153014. https:\/\/doi.org\/10.1371\/journal.pone.0153014","DOI":"10.1371\/journal.pone.0153014"},{"key":"10826_CR31","doi-asserted-by":"publisher","first-page":"e01262","DOI":"10.1128\/aac.01262-24","volume":"669","author":"Z He","year":"2024","unstructured":"He Z, Huang Y, Li W, Zhang H, Cao R, Ali MR et al (2024) Characterization and genomic analysis of the highly virulent Acinetobacter baumannii ST1791 strain dominating in anhui, China. Antimicrob Agents Chemother 669:e01262\u2013e01224. https:\/\/doi.org\/10.1128\/aac.01262-24","journal-title":"Antimicrob Agents Chemother"},{"key":"10826_CR32","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1007\/s10096-024-04974-w","volume":"44","author":"K Novovi\u0107","year":"2025","unstructured":"Novovi\u0107 K, Radovanovi\u0107 M, Gaji\u0107 I, Vasiljevi\u0107 Z, Male\u0161evi\u0107 M, \u0160api\u0107 K et al (2025) AdeABC, adefgh, and AdeIJK efflux pumps as key factors in Tigecycline resistance of Acinetobacter baumannii: a study from Western Balkan hospitals. Eur J Clin Microbiol Infect Dis 44:129\u2013142. https:\/\/doi.org\/10.1007\/s10096-024-04974-w","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10826_CR33","doi-asserted-by":"publisher","first-page":"151464","DOI":"10.1016\/j.ijmm.2020.151464","volume":"310","author":"Q Xu","year":"2020","unstructured":"Xu Q, Hua X, He J, Zhang D, Chen Q, Zhang L et al (2020) The distribution of mutations and hotspots in transcription regulators of resistance-nodulation-cell division efflux pumps in Tigecycline non-susceptible Acinetobacter baumannii in China. Int J Med Microbiol 310:151464. https:\/\/doi.org\/10.1016\/j.ijmm.2020.151464","journal-title":"Int J Med Microbiol"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-10826-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-025-10826-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-10826-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T04:26:21Z","timestamp":1766895981000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-025-10826-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,18]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10826"],"URL":"https:\/\/doi.org\/10.1007\/s11033-025-10826-3","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"type":"print","value":"0301-4851"},{"type":"electronic","value":"1573-4978"}],"subject":[],"published":{"date-parts":[[2025,7,18]]},"assertion":[{"value":"2 April 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The research project was exempted from the requirement for ethical approval because no health-related personal data were used.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"736"}}