{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:28:08Z","timestamp":1772119688714,"version":"3.50.1"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJSP2119"],"award-info":[{"award-number":["JPMJSP2119"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K02100"],"award-info":[{"award-number":["24K02100"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K02100"],"award-info":[{"award-number":["24K02100"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11033-025-10957-7","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T11:34:38Z","timestamp":1756380878000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microsatellite DNA markers for paternity identification in the hermaphroditic barnacle Chthamalus challengeri Hoek"],"prefix":"10.1007","volume":"52","author":[{"given":"Masami M.","family":"Tamechika","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoichi","family":"Yusa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shingo","family":"Akita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Sekino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"10957_CR1","doi-asserted-by":"publisher","first-page":"1377","DOI":"10.1111\/j.1558-5646.2009.00669.x","volume":"63","author":"L Sch\u00e4rer","year":"2009","unstructured":"Sch\u00e4rer L (2009) Tests of sex allocation theory in simultaneously hermaphroditic animals. Evol 63:1377\u20131405. https:\/\/doi.org\/10.1111\/j.1558-5646.2009.00669.x","journal-title":"Evol"},{"key":"10957_CR2","volume-title":"The theory of sex allocation","author":"EL Charnov","year":"1982","unstructured":"Charnov EL (1982) The theory of sex allocation. Princeton University Press, Princeton, New Jersey"},{"key":"10957_CR3","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1038\/hdy.1948.21","volume":"2","author":"AJ Bateman","year":"1948","unstructured":"Bateman AJ (1948) Intra-sexual selection in Drosophila. Heredity 2:349\u2013368. https:\/\/doi.org\/10.1038\/hdy.1948.21","journal-title":"Heredity"},{"key":"10957_CR4","doi-asserted-by":"publisher","first-page":"1206","DOI":"10.1086\/285278","volume":"138","author":"PT Raimondi","year":"1991","unstructured":"Raimondi PT, Martin JE (1991) Evidence that mating group size affects allocation of reproductive resources in a simultaneous hermaphrodite. Am Nat 138:1206\u20131217. https:\/\/doi.org\/10.1086\/285278","journal-title":"Am Nat"},{"key":"10957_CR5","doi-asserted-by":"publisher","first-page":"3233","DOI":"10.1111\/evo.12189","volume":"67","author":"T Janicke","year":"2013","unstructured":"Janicke T, Marie-Orleach L, De Mulder K, Berezikov E, Ladurner P, Vizoso DB, Sch\u00e4rer L (2013) Sex allocation adjustment to mating group size in a simultaneous hermaphrodite. Evolution 67:3233\u20133242. https:\/\/doi.org\/10.1111\/evo.12189","journal-title":"Evolution"},{"key":"10957_CR6","doi-asserted-by":"publisher","first-page":"3123","DOI":"10.1098\/rspb.2009.0566","volume":"276","author":"E Van Velzen","year":"2009","unstructured":"Van Velzen E, Sch\u00e4rer L, Pen I (2009) The effect of cryptic female choice on sex allocation in simultaneous hermaphrodites. Proc Biol Sci 276:3123\u20133131. https:\/\/doi.org\/10.1098\/rspb.2009.0566","journal-title":"Proc Biol Sci"},{"key":"10957_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ympev.2015.04.026","volume":"91","author":"HC Lin","year":"2015","unstructured":"Lin HC, H\u00f8eg JT, Yusa Y, Chan BK (2015) The origins and evolution of dwarf males and habitat use in thoracican barnacles. Mol Phylogenet Evol 91:1\u201311. https:\/\/doi.org\/10.1016\/j.ympev.2015.04.026","journal-title":"Mol Phylogenet Evol"},{"key":"10957_CR8","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1111\/j.1755-0998.2011.03014.x","volume":"11","author":"E Guichoux","year":"2011","unstructured":"Guichoux E, Lagache L, Wagner S, Chaumeil P, L\u00e9ger P, Lepais O, Lepoittevin C, Malausa T, Revardel E, Salin F, Petit RJ (2011) Current trends in microsatellite genotyping. Mol Ecol Resour 11:591\u2013611. https:\/\/doi.org\/10.1111\/j.1755-0998.2011.03014.x","journal-title":"Mol Ecol Resour"},{"key":"10957_CR9","volume-title":"Barnacles: structure, function, development and evolution","author":"DT Anderson","year":"1993","unstructured":"Anderson DT (1993) Barnacles: structure, function, development and evolution. Chapman and Hall, London"},{"key":"10957_CR10","doi-asserted-by":"publisher","first-page":"1585","DOI":"10.1111\/j.1365-294X.2010.04588.x","volume":"19","author":"MN Dawson","year":"2010","unstructured":"Dawson MN, Grosberg RK, Stuart YE, Sanford E (2010) Population genetic analysis of a recent range expansion: mechanisms regulating the poleward range limit in the volcano barnacle Tetraclita rubescens. Mol Ecol 19:1585\u20131605. https:\/\/doi.org\/10.1111\/j.1365-294X.2010.04588.x","journal-title":"Mol Ecol"},{"key":"10957_CR11","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1093\/jhered\/est064","volume":"105","author":"LV Plough","year":"2014","unstructured":"Plough LV, Marko PB (2014) Characterization of microsatellite loci and repeat density in the gooseneck barnacle, Pollicipes elegans, using next generation sequencing. J Hered 105:136\u2013142. https:\/\/doi.org\/10.1093\/jhered\/est064","journal-title":"J Hered"},{"key":"10957_CR12","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.bse.2015.09.015","volume":"63","author":"HN Chen","year":"2015","unstructured":"Chen HN, Chan BK, Tsang LM (2015) Transcriptome derived microsatellite markers of Tetraclita kuroshioensis and cross amplification among Tetraclita spp. Biochem Syst Ecol 63:13\u201316. https:\/\/doi.org\/10.1016\/j.bse.2015.09.015","journal-title":"Biochem Syst Ecol"},{"key":"10957_CR13","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2019","volume":"4","author":"C Ewers-Saucedo","year":"2016","unstructured":"Ewers-Saucedo C, Zardus JD, Wares JP (2016) Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle Chelonibia testudinaria (Linnaeus 1758). PeerJ 4:e2019. https:\/\/doi.org\/10.7717\/peerj.2019","journal-title":"PeerJ"},{"key":"10957_CR14","doi-asserted-by":"publisher","first-page":"4885","DOI":"10.1007\/s11033-020-05473-9","volume":"47","author":"M Kobayashi","year":"2020","unstructured":"Kobayashi M, Yusa Y, Sekino M (2020) Microsatellite DNA markers applicable to paternity inference in the androdioecious gooseneck barnacle Octolasmis Warwickii (Lepadiformes: Poecilasmatidae). Mol Biol Rep 47:4885\u20134890. https:\/\/doi.org\/10.1007\/s11033-020-05473-9","journal-title":"Mol Biol Rep"},{"key":"10957_CR15","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1017\/S0025315403007835h","volume":"83","author":"AJ Southward","year":"2003","unstructured":"Southward AJ, Newman WA (2003) A review of some common Indo-Malayan and Western Pacific species of Chthamalus barnacles (Crustacea: Cirripedia). J Mar Biol Assoc U K 83:797\u2013812. https:\/\/doi.org\/10.1017\/S0025315403007835h","journal-title":"J Mar Biol Assoc U K"},{"key":"10957_CR16","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1093\/jeb\/voaf007","volume":"38","author":"MM Tamechika","year":"2025","unstructured":"Tamechika MM, Yamada H, Ijiri S, Yusa Y (2025) The effects of parasitism on sex allocation of a hermaphroditic acorn barnacle. J Evol Biol 38:417\u2013429. https:\/\/doi.org\/10.1093\/jeb\/voaf007","journal-title":"J Evol Biol"},{"key":"10957_CR17","doi-asserted-by":"publisher","first-page":"727","DOI":"10.2331\/fishsci.62.727","volume":"62","author":"T Asahida","year":"1996","unstructured":"Asahida T, Kobayashi T, Saitoh K, Nakayama I (1996) Tissue preservation and total DNA extraction form fish stored at ambient temperature using buffers containing high concentration of urea. Fish Sci 62:727\u2013730. https:\/\/doi.org\/10.2331\/fishsci.62.727","journal-title":"Fish Sci"},{"key":"10957_CR18","volume-title":"Molecular cloning: A laboratory manual","author":"J Sambrook","year":"1989","unstructured":"Sambrook J, Fritsch EF, Manitaris T (1989) Molecular cloning: A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York","edition":"2"},{"key":"10957_CR19","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1007\/s10228-012-0317-8","volume":"60","author":"Y Nakamura","year":"2013","unstructured":"Nakamura Y, Shigenobu Y, Sugaya T, Kurokawa T, Saitoh K (2013) Automated screening and primer design of fish microsatellite DNA loci on pyrosequencing data. Ichthyol Res 60:184\u2013187. https:\/\/doi.org\/10.1007\/s10228-012-0317-8","journal-title":"Ichthyol Res"},{"key":"10957_CR20","doi-asserted-by":"publisher","first-page":"25","DOI":"10.2144\/01311bm02","volume":"31","author":"I Boutin-Ganache","year":"2001","unstructured":"Boutin-Ganache I, Raposo M, Raymond M, Deschepper CF (2001) M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods. Biotechniques 31:25\u201328. https:\/\/doi.org\/10.2144\/01311bm02","journal-title":"Biotechniques"},{"key":"10957_CR21","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1111\/j.1755-0998.2010.02847.x","volume":"10","author":"L Excoffier","year":"2010","unstructured":"Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and windows. Mol Ecol Resour 10:564\u2013567. https:\/\/doi.org\/10.1111\/j.1755-0998.2010.02847.x","journal-title":"Mol Ecol Resour"},{"key":"10957_CR22","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1007\/s12686-011-9523-3","volume":"4","author":"M Sekino","year":"2012","unstructured":"Sekino M, Kakehi S (2012) PARFEX v1. 0: an EXCEL\u2122-based software package for parentage allocation. Conserv Genet Resour 4:275\u2013278. https:\/\/doi.org\/10.1007\/s12686-011-9523-3","journal-title":"Conserv Genet Resour"},{"key":"10957_CR23","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","volume":"57","author":"Y Benjamini","year":"1995","unstructured":"Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc Ser B Stat Methodol 57:289\u2013300. https:\/\/doi.org\/10.1111\/j.2517-6161.1995.tb02031.x","journal-title":"J R Stat Soc Ser B Stat Methodol"},{"key":"10957_CR24","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1046\/j.1365-294x.1998.00374.x","volume":"7","author":"TC Marshall","year":"1998","unstructured":"Marshall TC, Slate JBK, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639\u2013655. https:\/\/doi.org\/10.1046\/j.1365-294x.1998.00374.x","journal-title":"Mol Ecol"},{"key":"10957_CR25","doi-asserted-by":"publisher","first-page":"5088","DOI":"10.1111\/mec.12009","volume":"21","author":"MW Kelly","year":"2012","unstructured":"Kelly MW, Grosberg RK, Sanford E (2012) Love the one you\u2019re with: proximity determines paternity success in the barnacle Tetraclita rubescens. Mol Ecol 21:5088\u20135097. https:\/\/doi.org\/10.1111\/mec.12009","journal-title":"Mol Ecol"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-10957-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-025-10957-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-10957-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T05:04:45Z","timestamp":1766898285000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-025-10957-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,28]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10957"],"URL":"https:\/\/doi.org\/10.1007\/s11033-025-10957-7","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-7024057\/v1","asserted-by":"object"}]},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,28]]},"assertion":[{"value":"2 July 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 August 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 August 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The experiments were conducted by following the National University Corporation Hokkaido University Regulations on Animal Experimentation.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable as our study species was not human.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not applicable as our study species was not human.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"<!--Emphasis Type='Bold' removed-->Consent to publication"}}],"article-number":"853"}}