{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T03:30:03Z","timestamp":1793676603972,"version":"4.5.1"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:00Z","timestamp":1760140800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:00Z","timestamp":1760140800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Global Minds PhD Scholarship program","award":["4520196970"],"award-info":[{"award-number":["4520196970"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11033-025-11121-x","type":"journal-article","created":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:08:55Z","timestamp":1760162935000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Harnessing eDNA metabarcoding for mosquito surveillance: a pilot study in Ethiopia\u2019s Great Rift Valley"],"prefix":"10.1007","volume":"52","author":[{"given":"Samson","family":"Leta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tesfaye Rufael","family":"Chibssa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob","family":"Lavigne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Paeshuyse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,11]]},"reference":[{"key":"11121_CR1","unstructured":"WHO (2017) Global vector control response 2017\u20132030. Geneva. Licence: CC BY-NC-SA 3.0 IGO."},{"key":"11121_CR2","unstructured":"WHO (2024) World malaria report 2024. (accessed 03 July 2025); https:\/\/www.who.int\/teams\/global-malaria-programme"},{"key":"11121_CR3","unstructured":"WHO (2020) Vector-borne diseases. https:\/\/www.who.int\/en\/news-room\/fact-sheets\/detail\/vector-borne-diseases. Accessed 2 Sep 2024"},{"key":"11121_CR4","doi-asserted-by":"publisher","first-page":"3989","DOI":"10.1016\/j.vaccine.2020.04.011","volume":"38","author":"M Girard","year":"2020","unstructured":"Girard M, Nelson CB, Picot V, Gubler DJ (2020) Arboviruses: a global public health threat. Vaccine 38:3989\u20133994. https:\/\/doi.org\/10.1016\/j.vaccine.2020.04.011","journal-title":"Vaccine"},{"issue":"8","key":"11121_CR5","doi-asserted-by":"publisher","first-page":"e000","DOI":"10.1101\/040386","volume":"10","author":"C Carlson","year":"2016","unstructured":"Carlson C, Dougherty E, Getz W (2016) An ecological assessment of the pandemic threat of Zika virus. PLoS NeglTrop Dis 10(8):e000. https:\/\/doi.org\/10.1101\/040386","journal-title":"PLoS NeglTrop Dis"},{"key":"11121_CR6","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1038\/nature12060","volume":"496","author":"S Bhatt","year":"2013","unstructured":"Bhatt S, Gething PW, Brady OJ et al (2013) The global distribution and burden of dengue. Nature 496:504\u2013507. https:\/\/doi.org\/10.1038\/nature12060","journal-title":"Nature"},{"key":"11121_CR7","doi-asserted-by":"publisher","first-page":"662","DOI":"10.1111\/1469-0691.12694","volume":"20","author":"RN Charrel","year":"2014","unstructured":"Charrel RN, Leparc-Goffart I, Gallian P, de Lamballerie X (2014) Globalization of chikungunya: 10 years to invade the world. Clin Microbiol Infect 20:662\u2013663. https:\/\/doi.org\/10.1111\/1469-0691.12694","journal-title":"Clin Microbiol Infect"},{"key":"11121_CR8","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1016\/S1473-3099(11)70147-5","volume":"11","author":"ES Jentes","year":"2011","unstructured":"Jentes ES, Poumerol G, Gershman MD et al (2011) The revised global yellow fever risk map and recommendations for vaccination, 2010: consensus of the informal WHO working group on geographic risk for yellow fever. Lancet Infect Dis 11:622\u2013632","journal-title":"Lancet Infect Dis"},{"key":"11121_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3402\/iee.v5.28024","volume":"5","author":"MO Nanyingi","year":"2015","unstructured":"Nanyingi MO, Munyua P, Kiama SG et al (2015) A systematic review of rift Valley fever epidemiology 1931\u20132014. Infect Ecol Epidemiol 5:1\u201312. https:\/\/doi.org\/10.3402\/iee.v5.28024","journal-title":"Infect Ecol Epidemiol"},{"key":"11121_CR10","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.ijid.2017.11.026","volume":"67","author":"S Leta","year":"2018","unstructured":"Leta S, Beyene TJ, De Clercq EM et al (2018) Global risk mapping for major diseases transmitted by Aedes aegypti and Aedes albopictus. Int J Infect Dis 67:25\u201335. https:\/\/doi.org\/10.1016\/j.ijid.2017.11.026","journal-title":"Int J Infect Dis"},{"key":"11121_CR11","doi-asserted-by":"publisher","DOI":"10.1186\/s12942-018-0125-0","author":"E Ducheyne","year":"2018","unstructured":"Ducheyne E, Tran Minh NN, Haddad N et al (2018) Current and future distribution of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in WHO Eastern mediterranean region. Int J Health Geogr. https:\/\/doi.org\/10.1186\/s12942-018-0125-0","journal-title":"Int J Health Geogr"},{"key":"11121_CR12","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6726622","author":"F Adugna","year":"2021","unstructured":"Adugna F, Wale M, Nibret E (2021) Review of Anopheles mosquito species, abundance, and distribution in Ethiopia. J Trop Med. https:\/\/doi.org\/10.1155\/2021\/6726622","journal-title":"J Trop Med"},{"key":"11121_CR13","doi-asserted-by":"publisher","first-page":"2716","DOI":"10.1002\/vms3.941","volume":"8","author":"MB Jaleta","year":"2022","unstructured":"Jaleta MB, Tefera M, Negussie H et al (2022) Entomological survey of the potential vectors of Rift Valley fever virus and absence of detection of the virus genome from the vectors in various niches in the Southern half of the Great Rift Valley of Ethiopia. Vet Med Sci 8:2716\u20132725. https:\/\/doi.org\/10.1002\/vms3.941","journal-title":"Vet Med Sci"},{"key":"11121_CR14","doi-asserted-by":"publisher","first-page":"4803","DOI":"10.1002\/ece3.7382","volume":"11","author":"J Fediajevaite","year":"2021","unstructured":"Fediajevaite J, Priestley V, Arnold R, Savolainen V (2021) Meta-analysis shows that environmental DNA outperforms traditional surveys, but warrants better reporting standards. Ecol Evol 11:4803\u20134815. https:\/\/doi.org\/10.1002\/ece3.7382","journal-title":"Ecol Evol"},{"key":"11121_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2022.e00183","volume":"29","author":"ME Sengupta","year":"2022","unstructured":"Sengupta ME, Lynggaard C, Mukaratirwa S et al (2022) Environmental DNA in human and veterinary parasitology - current applications and future prospects for monitoring and control. Food Waterborne Parasitol 29:e00183. https:\/\/doi.org\/10.1016\/j.fawpar.2022.e00183","journal-title":"Food Waterborne Parasitol"},{"key":"11121_CR16","unstructured":"WHO (2022) Manual for monitoring insecticide resistance in mosquito vectors and selecting appropriate interventions. Geneva"},{"key":"11121_CR17","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1186\/1756-3305-5-69","volume":"5","author":"ME Sinka","year":"2012","unstructured":"Sinka ME, Bangs MJ, Manguin S et al (2012) A global map of dominant malaria vectors. Parasit Vectors 5:69. https:\/\/doi.org\/10.1186\/1756-3305-5-69","journal-title":"Parasit Vectors"},{"key":"11121_CR18","unstructured":"Winding A, Bang-Andreasen T, Hansen LH et al (2019) eDNA in environmrntal monitoring"},{"key":"11121_CR19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnc.2022.126325","volume":"71","author":"A Sahu","year":"2023","unstructured":"Sahu A, Kumar N, Pal Singh C, Singh M (2023) Environmental DNA (eDNA): powerful technique for biodiversity conservation. J Nat Conserv 71:126325","journal-title":"J Nat Conserv"},{"key":"11121_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0162493","volume":"11","author":"J Schneider","year":"2016","unstructured":"Schneider J, Valentini A, Dejean T et al (2016) Detection of invasive mosquito vectors using environmental DNA (eDNA) from water samples. PLoS ONE 11:1\u201318. https:\/\/doi.org\/10.1371\/journal.pone.0162493","journal-title":"PLoS ONE"},{"key":"11121_CR21","doi-asserted-by":"publisher","first-page":"2031","DOI":"10.1111\/J.1365-294X.2012.05505.X","volume":"21","author":"NG Yoccoz","year":"2012","unstructured":"Yoccoz NG (2012) The future of environmental DNA in ecology. Mol Ecol 21:2031\u20132038. https:\/\/doi.org\/10.1111\/J.1365-294X.2012.05505.X","journal-title":"Mol Ecol"},{"key":"11121_CR22","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/J.BIOCON.2014.11.019","volume":"183","author":"PF Thomsen","year":"2015","unstructured":"Thomsen PF, Willerslev E (2015) Environmental DNA \u2013 an emerging tool in conservation for monitoring past and present biodiversity. Biol Conserv 183:4\u201318. https:\/\/doi.org\/10.1016\/J.BIOCON.2014.11.019","journal-title":"Biol Conserv"},{"key":"11121_CR23","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1098\/rsbl.2008.0118","volume":"4","author":"GF Ficetola","year":"2008","unstructured":"Ficetola GF, Miaud C, Pompanon F, Taberlet P (2008) Species detection using environmental DNA from water samples. Biol Lett 4:423\u2013425. https:\/\/doi.org\/10.1098\/rsbl.2008.0118","journal-title":"Biol Lett"},{"key":"11121_CR24","volume":"17","author":"KM Ruppert","year":"2019","unstructured":"Ruppert KM, Kline RJ, Rahman MS (2019) Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: a systematic review in methods, monitoring, and applications of global eDNA. Glob Ecol Conserv 17:e00547","journal-title":"Glob Ecol Conserv"},{"key":"11121_CR25","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1899\/13-046.1","volume":"32","author":"CS Goldberg","year":"2013","unstructured":"Goldberg CS, Sepulveda A, Ray A et al (2013) Environmental DNA as a new method for early detection of new Zealand mudsnails (Potamopyrgus antipodarum). Freshwater Sci 32:792\u2013800. https:\/\/doi.org\/10.1899\/13-046.1","journal-title":"Freshwater Sci"},{"key":"11121_CR26","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1111\/j.1755-263X.2010.00158.x","volume":"4","author":"CL Jerde","year":"2011","unstructured":"Jerde CL, Mahon AR, Chadderton WL, Lodge DM (2011) Sight-unseen detection of rare aquatic species using environmental DNA. Conserv Lett 4:150\u2013157. https:\/\/doi.org\/10.1111\/j.1755-263X.2010.00158.x","journal-title":"Conserv Lett"},{"key":"11121_CR27","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1111\/2041-210X.12206","volume":"5","author":"CR Turner","year":"2014","unstructured":"Turner CR, Barnes MA, Xu CCY et al (2014) Particle size distribution and optimal capture of aqueous macrobial eDNA. Methods Ecol Evol 5:676\u2013684. https:\/\/doi.org\/10.1111\/2041-210X.12206","journal-title":"Methods Ecol Evol"},{"key":"11121_CR28","doi-asserted-by":"publisher","first-page":"R1250","DOI":"10.1016\/j.cub.2022.09.052","volume":"32","author":"EA Didaskalou","year":"2022","unstructured":"Didaskalou EA, Trimbos KB, Stewart KA (2022) Environmental DNA. Curr Biol 32:R1250\u2013R1252. https:\/\/doi.org\/10.1016\/j.cub.2022.09.052","journal-title":"Curr Biol"},{"key":"11121_CR29","doi-asserted-by":"publisher","first-page":"1816","DOI":"10.1111\/j.1365-294X.2011.05317.x","volume":"21","author":"P Taberlet","year":"2012","unstructured":"Taberlet P, Prud\u2019Homme SM, Campione E et al (2012) Soil sampling and isolation of extracellular DNA from large amount of starting material suitable for metabarcoding studies. Mol Ecol 21:1816\u20131820. https:\/\/doi.org\/10.1111\/j.1365-294X.2011.05317.x","journal-title":"Mol Ecol"},{"key":"11121_CR30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1136\/bmjgh-2024-016589","volume":"9","author":"ST Bangoura","year":"2024","unstructured":"Bangoura ST, Sidib\u00e9 S, Kaba L et al (2024) Seroprevalence of seven arboviruses of public health importance in sub- saharan africa: a systematic review and meta- analysis. BMJ Glob Heal 9:1\u201312. https:\/\/doi.org\/10.1136\/bmjgh-2024-016589","journal-title":"BMJ Glob Heal"},{"key":"11121_CR31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13071-022-05303-w","volume":"15","author":"I Deblauwe","year":"2022","unstructured":"Deblauwe I, De Wolf K, De Witte J et al (2022) From a long-distance threat to the invasion front: a review of the invasive Aedes mosquito species in Belgium between 2007 and 2020. Parasit Vectors 15:1\u201317. https:\/\/doi.org\/10.1186\/s13071-022-05303-w","journal-title":"Parasit Vectors"},{"key":"11121_CR32","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1111\/jvec.12381","volume":"45","author":"E Merdi\u0107","year":"2020","unstructured":"Merdi\u0107 E, Klobu\u010dar A, \u017ditko T et al (2020) Updated checklist of the mosquitoes (Diptera: Culicidae) of Croatia. J Vector Ecol 45:135\u2013139. https:\/\/doi.org\/10.1111\/jvec.12381","journal-title":"J Vector Ecol"},{"key":"11121_CR33","first-page":"294","volume":"3","author":"O Folmer","year":"1994","unstructured":"Folmer O, Black M, Hoeh W et al (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3:294\u2013299","journal-title":"Mol Mar Biol Biotechnol"},{"key":"11121_CR34","doi-asserted-by":"publisher","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","volume":"28","author":"M Kearse","year":"2012","unstructured":"Kearse M, Moir R, Wilson A et al (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647\u20131649. https:\/\/doi.org\/10.1093\/bioinformatics\/bts199","journal-title":"Bioinformatics"},{"key":"11121_CR35","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1093\/molbev\/mst010","volume":"30","author":"K Katoh","year":"2013","unstructured":"Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772\u2013780. https:\/\/doi.org\/10.1093\/molbev\/mst010","journal-title":"Mol Biol Evol"},{"key":"11121_CR36","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1093\/oxfordjournals.molbev.a040023","volume":"10","author":"K Tamura","year":"1993","unstructured":"Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512\u2013526. https:\/\/doi.org\/10.1093\/oxfordjournals.molbev.a040023","journal-title":"Mol Biol Evol"},{"key":"11121_CR37","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1111\/J.0269-283X.2005.00564.X","volume":"19","author":"M Diallo","year":"2005","unstructured":"Diallo M, Nabeth P, Ba K et al (2005) Mosquito vectors of the 1998\u20131999 outbreak of rift Valley fever and other arboviruses (Bagaza, Sanar, Wesselsbron and West Nile) in Mauritania and Senegal. Med Vet Entomol 19:119\u2013126. https:\/\/doi.org\/10.1111\/J.0269-283X.2005.00564.X","journal-title":"Med Vet Entomol"},{"key":"11121_CR38","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/1475-2875-12-13","volume":"12","author":"JM Mwangangi","year":"2013","unstructured":"Mwangangi JM, Mbogo CM, Orindi BO et al (2013) Shifts in malaria vector species composition and transmission dynamics along the Kenyan Coast over the past 20 years. Malar J 12:13. https:\/\/doi.org\/10.1186\/1475-2875-12-13","journal-title":"Malar J"},{"key":"11121_CR39","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04282-0","author":"J Goupeyou-Youmsi","year":"2020","unstructured":"Goupeyou-Youmsi J, Rakotondranaivo T, Puchot N et al (2020) Differential contribution of Anopheles coustani and Anopheles arabiensis to the transmission of Plasmodium falciparum and Plasmodium vivax in two neighbouring villages of Madagascar. Parasit Vectors. https:\/\/doi.org\/10.1186\/s13071-020-04282-0","journal-title":"Parasit Vectors"},{"key":"11121_CR40","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-014-0532-z","author":"T Degefa","year":"2015","unstructured":"Degefa T, Zeynudin A, Godesso A et al (2015) Malaria incidence and assessment of entomological indices among resettled communities in Ethiopia: a longitudinal study. Malar J. https:\/\/doi.org\/10.1186\/s12936-014-0532-z","journal-title":"Malar J"},{"key":"11121_CR41","doi-asserted-by":"publisher","DOI":"10.1080\/2331205x.2021.1940639","author":"AB Orok","year":"2021","unstructured":"Orok AB, Ajibaye O, Aina OO et al (2021) Malaria interventions and control programes in sub-Saharan Africa: a narrative review. Cogent Med. https:\/\/doi.org\/10.1080\/2331205x.2021.1940639","journal-title":"Cogent Med"},{"issue":"2","key":"11121_CR42","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1111\/mve.12291","volume":"32","author":"TNJJ Nepomichene","year":"2018","unstructured":"Nepomichene TNJJ, Raharimalala FN, Andriamandimby SF et al (2018) Vector competence of culex antennatus and anopheles coustani mosquitoes for rift Valley fever virus in Madagascar. Med Vet Entomol 32(2):259\u2013262. https:\/\/doi.org\/10.1111\/mve.12291","journal-title":"Med Vet Entomol"},{"key":"11121_CR43","doi-asserted-by":"publisher","first-page":"e0005933","DOI":"10.1371\/journal.pntd.0005933","volume":"11","author":"Y Epelboin","year":"2017","unstructured":"Epelboin Y, Talaga S, Epelboin L, Dusfour I (2017) Zika virus: an updated review of competent or naturally infected mosquitoes. PLoS Negl Trop Dis 11:e0005933","journal-title":"PLoS Negl Trop Dis"},{"key":"11121_CR44","doi-asserted-by":"publisher","first-page":"385","DOI":"10.11646\/zootaxa.4772.2.10","volume":"4772","author":"S Azari-Hamidian","year":"2020","unstructured":"Azari-Hamidian S, Abai MR, Norouzi B (2020) Mansonia uniformis (Diptera: Culicidae), a genus and species new to Southwestern Asia, with a review of its medical and veterinary importance. Zootaxa 4772:385\u2013395","journal-title":"Zootaxa"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-11121-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-025-11121-x","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-11121-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T05:04:26Z","timestamp":1766898266000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-025-11121-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,11]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["11121"],"URL":"https:\/\/doi.org\/10.1007\/s11033-025-11121-x","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,11]]},"assertion":[{"value":"31 July 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 September 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 October 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"1010"}}