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This study investigates the functional significance of circRNA ciR-01114, previously identified in porcine ovarian tissue, in modulating ovarian cell functions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>To evaluate the impact of ciR-01114 on the regulation of basic porcine ovarian granulosa cell functions, we performed experiments to increase (using an overexpressing vector) and decrease (using a shRNA vector) the expression of this circRNA. We assessed the relative expression levels of ciR-01114, cell viability, proliferation (measured by the accumulation of PCNA and cyclin B1), cytoplasmic (assessed via the accumulation of bax and caspase-3), and nuclear (DNA fragmentation) apoptosis, and the release of progesterone, testosterone, estradiol, IGF-I, and oxytocin. The overexpression of ciR-01114 significantly enhanced cell viability, proliferation, and the release of progesterone, testosterone, estradiol, and oxytocin, while suppressing apoptosis. Conversely, ciR-01114 knockdown yielded opposing effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These preliminary findings suggest that ciR-01114 may regulate porcine ovarian cell functions, including cell cycle progression, apoptosis, and secretory activity. However, further validation and in-depth studies are necessary to confirm these observations and fully understand the mechanisms underlying ciR-01114\u2019s role in ovarian biology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11033-025-11126-6","type":"journal-article","created":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:00:07Z","timestamp":1760173207000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the epigenetic role of the circular RNA ciR-01114 in porcine ovarian cell functions"],"prefix":"10.1007","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5063-6344","authenticated-orcid":false,"given":"Zuzana","family":"Fabov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbora","family":"Loncov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdel Halim","family":"Harrath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anouar","family":"Feriani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander V.","family":"Sirotkin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,10,11]]},"reference":[{"key":"11126_CR1","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1186\/s40164-023-00451-w","volume":"12","author":"XY Feng","year":"2023","unstructured":"Feng XY, Zhu SX, Pu KJ et al (2023) New insight into circrnas: characterization, strategies, and biomedical applications. 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