{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T10:54:17Z","timestamp":1764154457981,"version":"3.46.0"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100004160","name":"Mazandaran University of Medical Sciences","doi-asserted-by":"crossref","award":["7064"],"award-info":[{"award-number":["7064"]}],"id":[{"id":"10.13039\/501100004160","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1007\/s11033-025-11297-2","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T10:31:43Z","timestamp":1764153103000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison the effect of Curcumin and methanolic extracts of Fraxinus excelsior L. and glycyrrhiza glabra L. on the expression of MexCD-OprJ and MexEF-OprN efflux pumps in multidrug resistant Pseudomonas aeruginosa clinical isolates"],"prefix":"10.1007","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0972-7324","authenticated-orcid":false,"given":"Zeynab","family":"Jamal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6042-6519","authenticated-orcid":false,"given":"Mehrdad","family":"Gholami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8769-9912","authenticated-orcid":false,"given":"Mohammad Ali","family":"Ebrahimzadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2932-1911","authenticated-orcid":false,"given":"Hamid Reza","family":"Goli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,11,26]]},"reference":[{"issue":"3","key":"11297_CR1","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1016\/j.imj.2023.05.003","volume":"2","author":"N Sathe","year":"2023","unstructured":"Sathe N, Beech P, Croft L, Suphioglu C, Kapat A, Athan E (2023) Pseudomonas aeruginosa: infections and novel approaches to treatment knowing the enemy the threat of Pseudomonas aeruginosa and exploring novel approaches to treatment. Infect Med 2(3):178\u2013194","journal-title":"Infect Med"},{"key":"11297_CR2","unstructured":"Singh MK (2019) Functional analysis of MexXY resistance nodulation division efflux pumps in Pseudomonas aeruginosa"},{"issue":"1","key":"11297_CR3","first-page":"45","volume":"79","author":"J Smith","year":"2024","unstructured":"Smith J, Johnson A, Davis L (2024) A significant challenge in treating Pseudomonas aeruginosa infections is their ability to adapt and develop resistance to many antibiotics. J Antimicrob Chemother 79(1):45\u201352","journal-title":"J Antimicrob Chemother"},{"key":"11297_CR4","unstructured":"Namjilsuren S (2019) The importance of RND-type efflux pumps in the interactions of Pseudomonas aeruginosa with 4-Methoxybenzaldehyde"},{"issue":"2","key":"11297_CR5","first-page":"1","volume":"3","author":"E Alt\u0131n\u00f6z","year":"2019","unstructured":"Alt\u0131n\u00f6z E, Altuner EM (2019) Antibiotic resistance and efflux pumps. J Infect Res 3(2):1\u20139","journal-title":"J Infect Res"},{"issue":"12","key":"11297_CR6","doi-asserted-by":"publisher","first-page":"4478","DOI":"10.1128\/AAC.01072-08","volume":"52","author":"S Fraud","year":"2008","unstructured":"Fraud S, Campigotto AJ, Chen Z, Poole K (2008) MexCD-OprJ multidrug efflux system of Pseudomonas aeruginosa: involvement in chlorhexidine resistance and induction by membrane-damaging agents dependent upon the AlgU stress response Sigma factor. Antimicrob Agents Chemother 52(12):4478\u20134482","journal-title":"Antimicrob Agents Chemother"},{"issue":"12","key":"11297_CR7","first-page":"993","volume":"9","author":"M Suresh","year":"2019","unstructured":"Suresh M, Narayanan N, Vimal KP, Jayasree PR, Kumar PR (2019) Mutational and phylogenetic analysis of NfxB gene in Multidrug-Resistant clinical isolates of Pseudomonas aeruginosa hyperexpressing MexCD-OprJ efflux pump. Am J Infect Microbiol 9(12):993\u2013999","journal-title":"Am J Infect Microbiol"},{"issue":"4","key":"11297_CR8","doi-asserted-by":"publisher","first-page":"2426","DOI":"10.1128\/AAC.02012-13","volume":"58","author":"I Martinez-Ramos","year":"2014","unstructured":"Martinez-Ramos I, Mulet X, Moya B, Barbier M, Oliver A, Alberti S (2014) Overexpression of MexCD-OprJ reduces Pseudomonas aeruginosa virulence by increasing its susceptibility to complement-mediated killing. Antimicrob Agents Chemother 58(4):2426\u20132429","journal-title":"Antimicrob Agents Chemother"},{"key":"11297_CR9","first-page":"undefined","volume":"20","author":"DI Islamieh","year":"2020","unstructured":"Islamieh DI, Goudarzi H, Khaledi A, Afshar D, Esmaeili D (2020) Reduced efflux pumps expression of Pseudomonas aeruginosa with satureja khuzistanica essential oil. J Med Sci 20:undefined","journal-title":"J Med Sci"},{"key":"11297_CR10","doi-asserted-by":"publisher","DOI":"10.3390\/biom10030352","author":"GE-S Batiha","year":"2020","unstructured":"Batiha GE-S, Beshbishy AM, El-Mleeh A, Abdel-Daim MM, Devkota HP (2020) Traditional uses, bioactive chemical constituents, and pharmacological and toxicological activities of Glycyrrhiza glabra L. (Fabaceae). Biol. https:\/\/doi.org\/10.3390\/biom10030352","journal-title":"Biol"},{"issue":"1","key":"11297_CR11","doi-asserted-by":"publisher","first-page":"14","DOI":"10.3390\/microorganisms4010014","volume":"16","author":"P Blanco","year":"2016","unstructured":"Blanco P, Hernando-Amado S, Reales-Calderon JA, Corona F, Lira F, Alcalde-Rico M et al (2016) Bacterial multidrug efflux pumps: much more than antibiotic resistance determinants. Microorganisms 16(1):14","journal-title":"Microorganisms"},{"issue":"6","key":"11297_CR12","first-page":"1249","volume":"27","author":"E Delik","year":"2024","unstructured":"Delik E, Ero\u011flu B, G\u00fclben M, Aykurt C, \u00d6zt\u00fcrk BE (2024) Evaluation of biological activities of various extracts of glaucium alakirensis, marrubium bourgaei, and peucedanum alpinum from T\u00fcrkiye. Kahramanmara\u015f S\u00fct\u00e7\u00fc \u0130mam \u00dcniversitesi Tar\u0131m ve Do\u011fa. Dergisi 27(6):1249\u20131261","journal-title":"Dergisi"},{"key":"11297_CR13","doi-asserted-by":"crossref","unstructured":"Dubale S, Kebebe D, Zeynudin A, Abdissa N, Suleman S Phytochemical screening and antimicrobial activity evaluation of selected medicinal plants in Ethiopia. J Experimental Pharmacol 2023 Dec 31:51\u201362","DOI":"10.2147\/JEP.S379805"},{"key":"11297_CR14","first-page":"39","volume":"2012","author":"EB Wallander","year":"2012","unstructured":"Wallander EB (2012) Systematics and floral evolution in fraxinus (Oleaceae). Bot J Linn Soc 2012:39\u201358","journal-title":"Bot J Linn Soc"},{"issue":"2","key":"11297_CR15","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.fitote.2006.08.002","volume":"78","author":"I Kostova","year":"2007","unstructured":"Kostova I, Iossifova T (2007) Chemical components of fraxinus species. Fitoterapia 78(2):85\u2013106","journal-title":"Fitoterapia"},{"issue":"5","key":"11297_CR16","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/S0367-326X(00)00340-3","volume":"72","author":"I Kostova","year":"2001","unstructured":"Kostova I (2001) Fraxinus Ornus L. Fitoterapia 72(5):471\u2013480","journal-title":"Fitoterapia"},{"issue":"5","key":"11297_CR17","first-page":"645","volume":"36","author":"Y-S Lee","year":"2011","unstructured":"Lee Y-S, Han S-H, Lee S-H, Kim Y-G, Park C-B, Kang O-H et al (2011) Synergistic effect of tetrandrine and ethidium bromide against methicillin-resistant Staphylococcus aureus (MRSA). Phytomedicine 36(5):645\u2013651","journal-title":"Phytomedicine"},{"issue":"8","key":"11297_CR18","doi-asserted-by":"publisher","first-page":"2978","DOI":"10.1167\/iovs.19-27200","volume":"60","author":"LD Hazlett","year":"2019","unstructured":"Hazlett LD, Ekanayaka SA, McClellan SA, Francis R (2019) Glycyrrhizin use for multi-drug resistant Pseudomonas aeruginosa: in vitro and in vivo studies. Invest Ophthalmol Vis Sci 60(8):2978\u20132989","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"3","key":"11297_CR19","first-page":"118","volume":"2","author":"F Sedighinia","year":"2012","unstructured":"Sedighinia F, Safipour Afshar A, Soleimanpour S, Zarif R, Asili J, Ghazvini K (2012) Antibacterial activity of glycyrrhiza glabra against oral pathogens: an in vitro study. Avicenna J Phytomed 2(3):118\u2013124","journal-title":"Avicenna J Phytomed"},{"issue":"1","key":"11297_CR20","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-017-1913-y","volume":"17","author":"T Siriyong","year":"2017","unstructured":"Siriyong T, Srimanote P, Chusri S, Yingyongnarongkul BE, Suaisom C, Tipmanee V et al (2017) Conessine as a novel inhibitor of multidrug efflux pump systems in Pseudomonas aeruginosa. BMC Complement Altern Med 17(1):405","journal-title":"BMC Complement Altern Med"},{"issue":"7","key":"11297_CR21","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1007\/s00284-023-03330-z","volume":"80","author":"Z Jamal","year":"2023","unstructured":"Jamal Z, Gholami M, Ebrahimzadeh MA, Goli HR (2023) The role of MexCD-OprJ and MexEF-OprN efflux systems in the multiple antibiotic resistance of Pseudomonas aeruginosa isolated from clinical samples. Curr Microbiol 80(7):221","journal-title":"Curr Microbiol"},{"issue":"2","key":"11297_CR22","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1556\/1886.2024.00035","volume":"14","author":"TJ Hossain","year":"2024","unstructured":"Hossain TJ (2024) Methods for screening and evaluation of antimicrobial activity: a review of protocols, advantages, and limitations. Eur J Microbiol Immunol 14(2):97\u2013115","journal-title":"Eur J Microbiol Immunol"},{"issue":"5","key":"11297_CR23","first-page":"327","volume":"9","author":"AJ Seukep","year":"2019","unstructured":"Seukep AJ, Kuete V, Nahar L, Sarker SD, Guo MJ (2019) Plant-derived secondary metabolites as the main source of efflux pump inhibitors and methods for identification. J Pharm Anal 9(5):327\u2013338","journal-title":"J Pharm Anal"},{"issue":"4","key":"11297_CR24","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2009\u2013 \u2206\u2206CT method. Methods 25(4):402\u2013408","journal-title":"Methods"},{"issue":"4","key":"11297_CR25","first-page":"229","volume":"8","author":"A Reza","year":"2019","unstructured":"Reza A, Sutton JM, Rahman KM (2019) Effectiveness of efflux pump inhibitors as biofilm disruptors and resistance breakers in gram-negative (ESKAPEE) bacteria. J Antimicrob Chemother 8(4):229","journal-title":"J Antimicrob Chemother"},{"issue":"1","key":"11297_CR26","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1038\/s43856-024-00591-y","volume":"4","author":"A Dulanto Chiang","year":"2024","unstructured":"Dulanto Chiang A, Dekker JP (2024) Efflux pump-mediated resistance to new beta lactam antibiotics in multidrug-resistant gram-negative bacteria. Commun Med 4(1):170","journal-title":"Commun Med"},{"issue":"1","key":"11297_CR27","doi-asserted-by":"publisher","first-page":"17","DOI":"10.32895\/UMP.MPR.9.1.2","volume":"9","author":"NT Duong","year":"2025","unstructured":"Duong NT, Huynh TM, Le AT (2025) Prevalence of overexpressed resistance nodulation division (RND) efflux pumps of Pseudomonas aeruginosa causing nosocomial infections in several hospitals in Ho Chi Minh City. MedPharmRes 9(1):17\u201328","journal-title":"MedPharmRes"},{"key":"11297_CR28","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1512472","volume":"15","author":"L Mazza","year":"2025","unstructured":"Mazza L, Bory A, Luscher A, Kloehn J, Wolfender JL, Van Delden C et al (2025) Multidrug efflux pumps of Pseudomonas aeruginosa show selectivity for their natural substrates. Front Microbiol 15:1512472","journal-title":"Front Microbiol"},{"issue":"3","key":"11297_CR29","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1016\/j.jiph.2017.09.016","volume":"11","author":"HR Goli","year":"2018","unstructured":"Goli HR, Nahaei MR, Rezaee MA, Hasani A, Kafil HS, Aghazadeh M et al (2018) Role of MexAB-OprM and MexXY-OprM efflux pumps and class 1 integrons in resistance to antibiotics in burn and Intensive Care Unit isolates of Pseudomonas aeruginosa. J Infect Public Health 11(3):364\u2013372","journal-title":"J Infect Public Health"},{"issue":"11","key":"11297_CR30","doi-asserted-by":"publisher","first-page":"2540","DOI":"10.1128\/AAC.41.11.2540","volume":"41","author":"T K\u00f6hler","year":"1997","unstructured":"K\u00f6hler T, Michea-Hamzehpour M, Plesiat P, Kahr AL, Pechere JC (1997) Differential selection of multidrug efflux systems by quinolones in Pseudomonas aeruginosa. Antimicrob Agents Chemother 41(11):2540\u20132543","journal-title":"Antimicrob Agents Chemother"},{"key":"11297_CR31","unstructured":"Prevalence of overexpressed Resistance nodulation division (RND) efflux pumps of Pseudomonas aeruginosa causing nosocomial infections in several hospitals in Ho Chi Minh City"},{"issue":"5","key":"11297_CR32","doi-asserted-by":"publisher","first-page":"1906","DOI":"10.1128\/AAC.01645-10","volume":"55","author":"G Cabot","year":"2011","unstructured":"Cabot G, Ocampo-Sosa AA, Tubau F, Macia MD, Rodr\u00edguez C, Moya B et al (2011) Overexpression of AmpC and efflux pumps in Pseudomonas aeruginosa isolates from bloodstream infections: prevalence and impact on resistance in a Spanish multicenter study. Antimicrob Agents Chemother 55(5):1906\u20131911","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"11297_CR33","first-page":"2","volume":"9","author":"SS Aghayan","year":"2017","unstructured":"Aghayan SS, Kalalian Mogadam H, Fazli M, Darban-Sarokhalil D, Khoramrooz SS, Jabalameli F et al (2017) The effects of Berberine and palmatine on efflux pumps Inhibition with different gene patterns in Pseudomonas aeruginosa isolated from burn infections. Avicenna J Med Biotech 9(1):2\u20137","journal-title":"Avicenna J Med Biotech"},{"issue":"2","key":"11297_CR34","doi-asserted-by":"publisher","first-page":"2395","DOI":"10.1007\/s11033-019-04700-2","volume":"46","author":"S Rahbar Takrami","year":"2019","unstructured":"Rahbar Takrami S, Ranji N, Sadeghizadeh M (2019) Antibacterial effects of Curcumin encapsulated in nanoparticles on clinical isolates of Pseudomonas aeruginosa through downregulation of efflux pumps. Mol Biol Rep 46(2):2395\u20132404","journal-title":"Mol Biol Rep"},{"issue":"2","key":"11297_CR35","doi-asserted-by":"publisher","first-page":"129","DOI":"10.4103\/ijmr.IJMR_2079_17","volume":"149","author":"A Sharma","year":"2019","unstructured":"Sharma A, Gupta VK, Pathania R (2019) Efflux pump inhibitors for bacterial pathogens: from bench to bedside. Indian J Med Res 149(2):129\u2013142","journal-title":"Indian J Med Res"},{"issue":"12","key":"11297_CR36","first-page":"855","volume":"9","author":"AE Ebbensgaard","year":"2020","unstructured":"Ebbensgaard AE, L\u00f8bner-Olesen A, Frimodt-M\u00f8ller J (2020) The role of efflux pumps in the transition from low-level to clinical antibiotic resistance. J Antimicrob Chemother 9(12):855\u2013865","journal-title":"J Antimicrob Chemother"},{"issue":"1","key":"11297_CR37","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1186\/s12906-018-2348-9","volume":"18","author":"T Siriyong","year":"2018","unstructured":"Siriyong T, Voravuthikunchai SP, Coote PJ (2018) Steroidal alkaloids and conessine from the medicinal plant holarrhena antidysenterica restore antibiotic efficacy in a galleria Mellonella model of multidrug-resistant Pseudomonas aeruginosa infection. BMC Complement Altern Med 18(1):285","journal-title":"BMC Complement Altern Med"},{"key":"11297_CR38","first-page":"98","volume":"98","author":"AS Chakotiya","year":"2016","unstructured":"Chakotiya AS, Tanwar A, Narula A, Sharma RK (2016) Alternative to antibiotics against Pseudomonas aeruginosa: effects of glycyrrhiza glabra on membrane permeability and inhibition of efflux activity and biofilm formation in Pseudomonas aeruginosa and its in vitro time-kill activity. J Med Plants 98:98\u2013105","journal-title":"J Med Plants"},{"issue":"2","key":"11297_CR39","doi-asserted-by":"publisher","first-page":"98","DOI":"10.3947\/ic.2015.47.2.98","volume":"47","author":"M Adabi","year":"2015","unstructured":"Adabi M, Talebi-Taher M, Arbabi L, Afshar M, Fathizadeh S, Minaeian S et al (2015) Spread of efflux pump overexpressing-mediated fluoroquinolone resistance and multidrug resistance in Pseudomonas aeruginosa by using an efflux pump inhibitor. Infect Chemother 47(2):98\u2013104","journal-title":"Infect Chemother"},{"issue":"7","key":"11297_CR40","first-page":"158","volume":"7","author":"X Yang","year":"2018","unstructured":"Yang X, Domalaon R, Lyu Y, Zhanel GG, Schweizer F (2018) Tobramycin-linked efflux pump inhibitor conjugates synergize fluoroquinolones, rifampicin and fosfomycin against multidrug-resistant Pseudomonas aeruginosa. J Clin Microbiol 7(7):158\u2013170","journal-title":"J Clin Microbiol"},{"issue":"7","key":"11297_CR41","doi-asserted-by":"publisher","first-page":"1935","DOI":"10.1021\/acs.jnatprod.9b00317","volume":"82","author":"E Laudadio","year":"2019","unstructured":"Laudadio E, Cedraro N, Mangiaterra G, Citterio B, Mobbili G, Minnelli C et al (2019) Natural alkaloid berberine activity against Pseudomonas aeruginosa MexXY-mediated aminoglycoside resistance: in silico and in vitro studies. J Nat Prod 82(7):1935\u20131944","journal-title":"J Nat Prod"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-11297-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-025-11297-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-11297-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T10:31:48Z","timestamp":1764153108000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-025-11297-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,26]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["11297"],"URL":"https:\/\/doi.org\/10.1007\/s11033-025-11297-2","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,26]]},"assertion":[{"value":"15 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 November 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no conflict of interest or competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This research was approved by ethics committee of Mazandaran University of Medical Sciences, Sari, Iran under the ethics application IR.MAZUMS.REC.1399.181.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Informed consent was obtained from all individual participants included in the study for publication of this research.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to publish"}}],"article-number":"127"}}