{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T07:59:31Z","timestamp":1768377571199,"version":"3.49.0"},"reference-count":15,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1007\/s11033-025-11426-x","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T15:50:30Z","timestamp":1768319430000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Melanin-free DNA extraction: a rapid and high-efficiency protocol for molecular studies"],"prefix":"10.1007","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5330-6968","authenticated-orcid":false,"given":"Sanae","family":"EL Bardai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatima","family":"EL Agy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Layla","family":"Tahiri Elousrouti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laila","family":"Bouguenouch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karim","family":"Ouldim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nawal","family":"Hammas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laila","family":"Chbani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,1,13]]},"reference":[{"key":"11426_CR1","doi-asserted-by":"publisher","first-page":"3488","DOI":"10.1038\/s41598-024-53941-0","volume":"14","author":"-Zamudio D\u00edaz","year":"2024","unstructured":"D\u00edaz -Zamudio, Busch DF, Kr\u00f6ger L (2024) Significance of melanin distribution in the epidermis for the protective effect against UV light. Sci Rep 14:3488. https:\/\/doi.org\/10.1038\/s41598-024-53941-0","journal-title":"Sci Rep"},{"key":"11426_CR2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-78782-9_106","volume-title":"Rossi, U. Advances in forensic haemogenetics. Advances in forensic haemogenetics","author":"T -Yoshii","year":"1994","unstructured":"-Yoshii T, Akiyama K, Tamura K, Ishiyama I (1994) PCR inhibitor: water-soluble melanin, which inhibits DNA polymerases and DNases. In: B\u00e4r W, Fiori A (eds) Rossi, U. Advances in forensic haemogenetics. Advances in forensic haemogenetics, vol 5. Springer, Berlin, Heidelberg. https:\/\/doi.org\/10.1007\/978-3-642-78782-9_106"},{"key":"11426_CR3","doi-asserted-by":"publisher","unstructured":"Schrader C, Schielke A, Ellerbroek L, Johne R (2012)  PCR inhibitors-occurrence, properties and removal. Journal of Applied Microbiology 113(5):1014\u20131026. https:\/\/doi.org\/10.1111\/j.1365-2672.2012.05384.x","DOI":"10.1111\/j.1365-2672.2012.05384.x"},{"key":"11426_CR4","doi-asserted-by":"publisher","first-page":"307","DOI":"10.3390\/jmp4040025","volume":"4","author":"SD Parsons","year":"2023","unstructured":"Parsons SD, Murphy K, Finall A (2023) Assessing the impact of melanin content on the reliability of the Idylla\u2122 BRAF mutation test. J Mol Pathol 4:307\u2013317. https:\/\/doi.org\/10.3390\/jmp4040025","journal-title":"J Mol Pathol"},{"key":"11426_CR5","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24054360","volume":"24","author":"L Guo","year":"2023","unstructured":"Guo L, Li W, Gu Z, Wang L, Guo L, Ma S, Li C, Sun J, Han B, Chang J (2023) Recent advances and progress on melanin: from source to application. Int J Mol Sci 24:4360. https:\/\/doi.org\/10.3390\/ijms24054360","journal-title":"Int J Mol Sci"},{"issue":"3","key":"11426_CR6","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1006\/bbrc.2000.2716","volume":"271","author":"L -Eckhart","year":"2000","unstructured":"-Eckhart L, Bach J, Ban J, Tschachler E (2000) Melanin binds reversibly to thermostable DNA polymerase and inhibits its activity. Biochem Biophys Res Commun 271(3):726\u2013730","journal-title":"Biochem Biophys Res Commun"},{"issue":"9","key":"11426_CR7","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.14670\/HH-18-112","volume":"34","author":"ALSA Vicente","year":"2019","unstructured":"Vicente ALSA, Bianchini RA, Laus AC, Macedo G, Reis RM, Vazquez VL (2019) Comparison of protocols for removal of melanin from genomic DNA to optimize PCR amplification of DNA purified from highly pigmented lesions. Histol Histopathol 34(9):1089\u20131096. https:\/\/doi.org\/10.14670\/HH-18-112","journal-title":"Histol Histopathol"},{"key":"11426_CR8","doi-asserted-by":"publisher","DOI":"10.17504\/protocols.io.eq2ly73mm","author":"D Buchner","year":"2022","unstructured":"Buchner D (2022) Guanidine-based DNA extraction with silica-coated beads or silica spin columns. Protocols io Eq.\u00a02ly73mmlx9. https:\/\/doi.org\/10.17504\/protocols.io.eq2ly73mm","journal-title":"Protocols Io Eq 2ly73mmlx9"},{"key":"11426_CR9","doi-asserted-by":"publisher","unstructured":"Gautam A (2022) DNA isolation by Chelex method. In: Gautam A (ed) DNA and RNA Isolation Techniques for Non-Experts. Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham, pp 181\u2013189. https:\/\/doi.org\/10.1007\/978-3-030-94230-4_10","DOI":"10.1007\/978-3-030-94230-4_10"},{"key":"11426_CR10","first-page":"506","volume":"10","author":"PS Walsh","year":"1991","unstructured":"Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506\u2013513","journal-title":"Biotechniques"},{"issue":"7","key":"11426_CR11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0158698","volume":"11","author":"E Frouin","year":"2016","unstructured":"Frouin E, Maudelonde T, Senal R, Larrieux M, Costes V, Godreuil S et al (2016) Comparative methods to improve the detection of BRAF V600 mutations in highly pigmented melanoma specimens. PLoS One 11(7):e0158698","journal-title":"PLoS One"},{"key":"11426_CR12","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.fsigen.2014.12.001","volume":"16","author":"Q Hu","year":"2015","unstructured":"Hu Q, Liu Y, Yi S, Huang D (2015) A comparison of four methods for PCR inhibitor removal. Forensic Sci Int Genet 16:94\u201397. https:\/\/doi.org\/10.1016\/j.fsigen.2014.12.001","journal-title":"Forensic Sci Int Genet"},{"key":"11426_CR13","doi-asserted-by":"publisher","first-page":"307","DOI":"10.3390\/jmp4040025","volume":"4","author":"SD -Parsons","year":"2023","unstructured":"-Parsons SD, Murphy K, Finall A (2023) Assessing the impact of melanin content on the reliability of the Idylla\u2122 BRAF mutation test. J Mol Pathol 4:307\u2013317. https:\/\/doi.org\/10.3390\/jmp4040025","journal-title":"J Mol Pathol"},{"key":"11426_CR14","doi-asserted-by":"publisher","unstructured":"-Tripathi G, Rathinam RB (2025) The challenges of PCR amplification in disease diagnosis. In Advances in Molecular Diagnostics and Therapeutics (Chap. 15). Springer. https:\/\/doi.org\/10.1007\/978-981-96-4620-3_15","DOI":"10.1007\/978-981-96-4620-3_15"},{"key":"11426_CR15","unstructured":"-Koetsier G, Cantor E (2023) A practical guide to analyzing nucleic acid concentration and purity with microvolume spectrophotometers. New England Biolabs"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-11426-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-025-11426-x","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-11426-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T03:03:57Z","timestamp":1768359837000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-025-11426-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,13]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["11426"],"URL":"https:\/\/doi.org\/10.1007\/s11033-025-11426-x","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,13]]},"assertion":[{"value":"22 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 December 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 January 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"This study was conducted in accordance with the institutional guidelines of the Hospital-University Ethics Committee of Fez (CERB Fez). Formal ethics committee approval was deemed unnecessary, as the report is based exclusively on a retrospective review of previously collected diagnostic data and does not involve interventional or experimental research on human subjects. Comprehensive written informed consent was obtained from living patients prior to publication.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}}],"article-number":"285"}}