{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:23:38Z","timestamp":1792020218333,"version":"4.4.0"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100025273","name":"Constantine the Philosopher University in Nitra","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100025273","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Circular RNAs (circRNAs) are stable epigenetic regulators of various biological processes, yet the functional role of ciR-02852 remains unknown. This study aimed to characterize the influence of ciR-02852 on the fundamental functions of porcine ovarian granulosa cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Cells were transfected with either a ciR-02852-overexpressing vector or an shRNA ciR-02852-knockdown vector. We assessed cell viability, proliferation markers (PCNA, cyclin B1), apoptosis markers (Bax, caspase-3, and DNA fragmentation), and the secretion of steroid (progesterone, testosterone, estradiol) and peptide (IGF-I, oxytocin) hormones via RT-qPCR, immunocytochemistry, TUNEL, and ELISA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Overexpression of ciR-02852 significantly reduced cell viability and the expression of PCNA and cyclin B1. Conversely, it stimulated both cytoplasmic and nuclear apoptosis, as evidenced by increased Bax, caspase-3, and DNA fragmentation. Furthermore, ciR-02852 overexpression inhibited the release of all measured hormones. Silencing of ciR-02852 via shRNA yielded the opposite effects, promoting proliferation and hormone output while suppressing apoptosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings demonstrate for the first time that ciR-02852 acts as a potent physiological inhibitor of porcine ovarian cell functions. Our results indicate that ciR-02852 could be a multi-target regulator of folliculogenesis and hormone production, highlighting its potential as a diagnostic marker or therapeutic target for managing reproductive disorders and improving animal production.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11033-026-11539-x","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T05:32:12Z","timestamp":1770615132000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Circular RNA ciR-02852: A novel physiological inhibitor of Porcine ovarian granulosa cell functions"],"prefix":"10.1007","volume":"53","author":[{"given":"Zuzana","family":"Fabov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbora","family":"Loncov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdel Halim","family":"Harrath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anouar","family":"Feriani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9364-3512","authenticated-orcid":false,"given":"Alexander V.","family":"Sirotkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,9]]},"reference":[{"key":"11539_CR1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-022-00992-3","volume":"20","author":"N Nouri","year":"2022","unstructured":"Nouri N, Shareghi-Oskoue O, Aghebati-Maleki L et al (2022) Role of miRNAs interference on ovarian functions and premature ovarian failure. 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