{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T21:57:00Z","timestamp":1781647020039,"version":"3.54.5"},"reference-count":18,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004434","name":"Universit\u00e0 degli Studi di Firenze","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004434","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Influenza virus isolation in cell cultures can be a foremost activity in the surveillance of the epidemiological characteristics involved in the vaccination strategies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Here, the virus isolation effectiveness from double positive throat swab to influenza A virus (H1N1pdm09 or H3N2) and SARS-CoV-2 compared to those obtained from single positive throat swab samples was investigated by serial passages of throat swabs samples in cell cultures. Viral replication and IFN-\u03b1, IL-1\u03b1, and IL-6 cytokine expression at three passages were measured by RT-PCR amplification. Virus isolation using a throat swab samples resulted double positive to influenza A virus (H1N1pdm09 or H3N2) and SARS-CoV-2 showed a different effectiveness compared to a throat samples single positive to each of these viruses. The virus isolation efficacy was independent from an active replication of the two viruses in the inoculum and showed a virus strain-specific reduction in cytokines profile compared to a single infection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Collectively the results point out to take into account the presence of co-circulating respiratory viruses in human samples for virus isolation effectiveness.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11033-026-11764-4","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:43:53Z","timestamp":1775198633000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reduction of virus isolation effectiveness from throat swab double positive to H1N1pdm09 and H3N2 influenza A virus strain with SARS-CoV-2 virus in cell cultures"],"prefix":"10.1007","volume":"53","author":[{"given":"Rosaria","family":"Arvia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Tassone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Giannecchini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,3]]},"reference":[{"key":"11764_CR1","doi-asserted-by":"publisher","first-page":"e00103","DOI":"10.1128\/CMR.00103-20","volume":"34","author":"N Clementi","year":"2021","unstructured":"Clementi N, Ghosh S, De Santis M, Castelli M, Criscuolo E, Zanoni I, Clementi M, Mancini N (2021) Viral respiratory pathogens and lung injury. 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The individuals are not identifiable and the samples analyzed were collected within routine respiratory virus diagnostic and surveillance activities.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical declaration"}}],"article-number":"586"}}