{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T09:01:15Z","timestamp":1781082075543,"version":"3.54.1"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"the Joint Program on Health Science & Technology Innovation of Hainan Province","award":["WSJK2025QN078"],"award-info":[{"award-number":["WSJK2025QN078"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1007\/s11033-026-12089-y","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T08:28:54Z","timestamp":1781080134000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Allicin inhibits kidney stone formation by suppressing the TNF\/NF-\u03baB pathway: Experimental validation through network pharmacology, in vitro, and in vivo studies"],"prefix":"10.1007","volume":"53","author":[{"given":"Zechun","family":"Peng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingbin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuming","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"key":"12089_CR1","doi-asserted-by":"crossref","unstructured":"Alelign T, Petros B (2018) Kidney Stone Disease: An Update on Current Concepts. Adv Urol, 2018: 3068365","DOI":"10.1155\/2018\/3068365"},{"issue":"9","key":"12089_CR2","doi-asserted-by":"publisher","first-page":"1301","DOI":"10.1007\/s00345-017-2008-6","volume":"35","author":"I Sorokin","year":"2017","unstructured":"Sorokin I, Mamoulakis C, Miyazawa K, Rodgers A, Talati J, Lotan Y (2017) Epidemiology of stone disease across the world. World J Urol 35(9):1301\u20131320","journal-title":"World J Urol"},{"issue":"6","key":"12089_CR3","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1038\/s41581-020-00392-1","volume":"17","author":"SR Khan","year":"2021","unstructured":"Khan SR, Canales BK, Dominguez-Gutierrez PR (2021) Randall\u2019s plaque and calcium oxalate stone formation: role for immunity and inflammation. Nat Rev Nephrol 17(6):417\u2013433","journal-title":"Nat Rev Nephrol"},{"issue":"1","key":"12089_CR4","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1186\/s12917-021-03023-4","volume":"17","author":"TK Abouzed","year":"2021","unstructured":"Abouzed TK, Sherif E, a E, Barakat MES, Sadek KM, Aldhahrani A, Nasr NE, Eldomany E, Khailo K, Dorghamm DA (2021) Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats. BMC Vet Res 17(1):350","journal-title":"BMC Vet Res"},{"issue":"16","key":"12089_CR5","doi-asserted-by":"publisher","first-page":"7319","DOI":"10.7150\/thno.44054","volume":"10","author":"H Liu","year":"2020","unstructured":"Liu H, Yang X, Tang K, Ye T, Duan C, Lv P, Yan L, Wu X, Chen Z, Liu J, Deng Y, Zeng G, Xing J, Ye Z, Xu H (2020) Sulforaphane elicts dual therapeutic effects on Renal Inflammatory Injury and crystal deposition in Calcium Oxalate Nephrocalcinosis. Theranostics 10(16):7319\u20137334","journal-title":"Theranostics"},{"issue":"3","key":"12089_CR6","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1016\/j.eururo.2015.07.040","volume":"69","author":"C T\u00fcrk","year":"2016","unstructured":"T\u00fcrk C, Pet\u0159\u00edk A, Sarica K, Seitz C, Skolarikos A, Straub M, Knoll T (2016) EAU Guidelines on Diagnosis and Conservative Management of Urolithiasis. Eur Urol 69(3):468\u2013474","journal-title":"Eur Urol"},{"issue":"4","key":"12089_CR7","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1517\/14656566.2013.775250","volume":"14","author":"H Xu","year":"2013","unstructured":"Xu H, Zisman AL, Coe FL, Worcester EM (2013) Kidney stones: an update on current pharmacological management and future directions. Expert Opin Pharmacother 14(4):435\u2013447","journal-title":"Expert Opin Pharmacother"},{"issue":"3","key":"12089_CR8","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1016\/j.juro.2006.10.023","volume":"177","author":"JK Parsons","year":"2007","unstructured":"Parsons JK, Hergan LA, Sakamoto K, Lakin C (2007) Efficacy of alpha-blockers for the treatment of ureteral stones. J Urol 177(3):983\u2013987 discussion 7","journal-title":"J Urol"},{"issue":"6","key":"12089_CR9","doi-asserted-by":"publisher","first-page":"2244","DOI":"10.1016\/j.juro.2008.01.141","volume":"179","author":"RN Pedro","year":"2008","unstructured":"Pedro RN, Hinck B, Hendlin K, Feia K, Canales BK, Monga M (2008) Alfuzosin stone expulsion therapy for distal ureteral calculi: a double-blind, placebo controlled study. J Urol 179(6):2244\u20132247 discussion 7","journal-title":"J Urol"},{"issue":"2","key":"12089_CR10","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1016\/j.juro.2014.05.006","volume":"192","author":"MS Pearle","year":"2014","unstructured":"Pearle MS, Goldfarb DS, Assimos DG, Curhan G, Denu-Ciocca CJ, Matlaga BR, Monga M, Penniston KL, Preminger GM, Turk TM, White JR (2014) Medical management of kidney stones: AUA guideline. J Urol 192(2):316\u2013324","journal-title":"J Urol"},{"key":"12089_CR11","doi-asserted-by":"crossref","unstructured":"Akram M, Jahrreiss V, Skolarikos A, Geraghty R, Tzelves L, Emilliani E, Davis NF (2024) Somani B K. Urological Guidelines for Kidney Stones: Overview and Comprehensive Update. J Clin Med, 13(4):1114.","DOI":"10.3390\/jcm13041114"},{"key":"12089_CR12","doi-asserted-by":"crossref","unstructured":"Ormanji MS, Rodrigues FG, Heilberg IP (2020) Dietary Recommendations for Bariatric Patients to Prevent Kidney Stone Formation. Nutrients, 12(5):1442.","DOI":"10.3390\/nu12051442"},{"key":"12089_CR13","doi-asserted-by":"crossref","unstructured":"Papatsoris A, Geavlete B, Radavoi GD, Alameedee M, Almusafer M, Ather MH, Budia A, Cumpanas AA, Kiremi MC, Dellis A, Elhowairis M, Gal\u00e1n-Llopis JA, Geavlete P, Guimer\u00e0 Garcia J, Isern B, Jinga V, Lopez JM, Mainez JA, Mitsogiannis I, Mora Christian J, Moussa M, Multescu R, Oguz Acar Y, Petkova K, Pi\u00f1ero A, Popov E, Ramos Cebrian M, Rascu S, Siener R, Sountoulides P, Stamatelou K, Syed J, Trinchieri A (2025) Management of urinary stones by experts in stone disease (ESD 2025), Arch Ital Urol Androl, 97:14085. 2","DOI":"10.4081\/aiua.2025.14085"},{"key":"12089_CR14","doi-asserted-by":"publisher","first-page":"38536","DOI":"10.1038\/srep38536","volume":"6","author":"P Peerapen","year":"2016","unstructured":"Peerapen P, Thongboonkerd V (2016) Caffeine prevents kidney stone formation by translocation of apical surface annexin A1 crystal-binding protein into cytoplasm: In vitro evidence. Sci Rep 6:38536","journal-title":"Sci Rep"},{"issue":"12","key":"12089_CR15","doi-asserted-by":"publisher","first-page":"2423","DOI":"10.3390\/ani10122423","volume":"10","author":"EM Awed","year":"2020","unstructured":"Awed EM, Sadek KM, Soliman MK, Khalil RH, Younis EM, Abdel-Warith A-WA, Van Doan H, Dawood MA, Abdel-Latif HM (2020) Spirulina platensis alleviated the oxidative damage in the gills, liver, and kidney organs of Nile tilapia intoxicated with sodium sulphate. Animals 10(12):2423","journal-title":"Animals"},{"issue":"1","key":"12089_CR16","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1007\/s43440-025-00801-x","volume":"78","author":"A Singh","year":"2026","unstructured":"Singh A, Singh L, Dalal D (2026) Formononetin as a multifaceted modulator of renal pathology: insights into fibrotic, oxidative, inflammatory, and apoptotic pathways. Pharmacol Rep 78(1):194\u2013208","journal-title":"Pharmacol Rep"},{"issue":"3","key":"12089_CR17","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1007\/s43440-025-00728-3","volume":"77","author":"D Dalal","year":"2025","unstructured":"Dalal D, Singh L, Singh A (2025) Calycosin and kidney health: a molecular perspective on its protective mechanisms. Pharmacol Rep 77(3):658\u2013669","journal-title":"Pharmacol Rep"},{"issue":"1","key":"12089_CR18","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1093\/advances\/nmy077","volume":"10","author":"R Kanlaya","year":"2019","unstructured":"Kanlaya R, Thongboonkerd V (2019) Protective Effects of Epigallocatechin-3-Gallate from Green Tea in Various Kidney Diseases. Adv Nutr 10(1):112\u2013121","journal-title":"Adv Nutr"},{"key":"12089_CR19","doi-asserted-by":"publisher","first-page":"115988","DOI":"10.1016\/j.biopha.2023.115988","volume":"170","author":"R Kanlaya","year":"2024","unstructured":"Kanlaya R, Kuljiratansiri R, Peerapen P, Thongboonkerd V (2024) The inhibitory effects of epigallocatechin-3-gallate on calcium oxalate monohydrate crystal growth, aggregation and crystal-cell adhesion. Biomed Pharmacother 170:115988","journal-title":"Biomed Pharmacother"},{"key":"12089_CR20","doi-asserted-by":"publisher","first-page":"e16132","DOI":"10.7717\/peerj.16132","volume":"11","author":"ASA Buendia","year":"2023","unstructured":"Buendia ASA, Rojas JGJ, Garc\u00eda-Arroyo F, Trejo OEA, S\u00e1nchez-Mu\u00f1oz F, Arg\u00fcello-Garc\u00eda R, S\u00e1nchez-Lozada LG, Bojalil R, Osorio-Alonso H (2023) Antioxidant and anti-inflammatory effects of allicin in the kidney of an experimental model of metabolic syndrome. PeerJ 11:e16132","journal-title":"PeerJ"},{"issue":"11","key":"12089_CR21","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1038\/nchembio.118","volume":"4","author":"AL Hopkins","year":"2008","unstructured":"Hopkins AL (2008) Network pharmacology: the next paradigm in drug discovery. Nat Chem Biol 4(11):682\u2013690","journal-title":"Nat Chem Biol"},{"issue":"2","key":"12089_CR22","doi-asserted-by":"publisher","first-page":"146","DOI":"10.2174\/157340911795677602","volume":"7","author":"XY Meng","year":"2011","unstructured":"Meng XY, Zhang HX, Mezei M, Cui M (2011) Molecular docking: a powerful approach for structure-based drug discovery. Curr Comput Aided Drug Des 7(2):146\u2013157","journal-title":"Curr Comput Aided Drug Des"},{"issue":"3","key":"12089_CR23","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1159\/000342333","volume":"36","author":"MM Speeckaert","year":"2012","unstructured":"Speeckaert MM, Speeckaert R, Laute M, Vanholder R, Delanghe JR (2012) Tumor necrosis factor receptors: biology and therapeutic potential in kidney diseases. Am J Nephrol 36(3):261\u2013270","journal-title":"Am J Nephrol"},{"issue":"1","key":"12089_CR24","doi-asserted-by":"publisher","first-page":"184394","DOI":"10.1016\/j.bbamem.2024.184394","volume":"1867","author":"CH Lo","year":"2025","unstructured":"Lo CH (2025) TNF receptors: Structure-function relationships and therapeutic targeting strategies. Biochim Biophys Acta Biomembr 1867(1):184394","journal-title":"Biochim Biophys Acta Biomembr"},{"issue":"5","key":"12089_CR25","doi-asserted-by":"publisher","first-page":"1199","DOI":"10.1016\/j.clnu.2004.03.011","volume":"23","author":"A Lang","year":"2004","unstructured":"Lang A, Lahav M, Sakhnini E, Barshack I, Fidder HH, Avidan B, Bardan E, Hershkoviz R, Bar-Meir S, Chowers Y (2004) Allicin inhibits spontaneous and TNF-alpha induced secretion of proinflammatory cytokines and chemokines from intestinal epithelial cells. Clin Nutr 23(5):1199\u20131208","journal-title":"Clin Nutr"},{"key":"12089_CR26","doi-asserted-by":"publisher","first-page":"173052","DOI":"10.1016\/j.ejphar.2020.173052","volume":"876","author":"CL Li","year":"2020","unstructured":"Li CL, Liu XH, Qiao Y, Ning LN, Li WJ, Sun YS, Liu DS, Gao W, Ma CM (2020) Allicin alleviates inflammation of diabetic macroangiopathy via the Nrf2 and NF-kB pathway. Eur J Pharmacol 876:173052","journal-title":"Eur J Pharmacol"},{"issue":"1","key":"12089_CR27","doi-asserted-by":"publisher","first-page":"187","DOI":"10.3390\/cimb43010016","volume":"43","author":"AH Alamir","year":"2021","unstructured":"Alamir AH, Patil S (2021) Allicin Could Potentially Alleviate Oral Cancer Pain by Inhibiting Pain Mediators TNF-alpha, IL-8, and Endothelin. Curr Issues Mol Biol 43(1):187\u2013196","journal-title":"Curr Issues Mol Biol"},{"key":"12089_CR28","doi-asserted-by":"publisher","first-page":"156500","DOI":"10.1016\/j.phymed.2025.156500","volume":"139","author":"Y Cheng","year":"2025","unstructured":"Cheng Y, Gu W, Wu X, Tian W, Mu Z, Ye Y, Chao H, Bao Z (2025) Allicin alleviates traumatic brain injury-induced neuroinflammation by enhancing PKC-\u03b4-mediated mitophagy. Phytomedicine 139:156500","journal-title":"Phytomedicine"},{"key":"12089_CR29","doi-asserted-by":"crossref","unstructured":"Hu J, Li C, Zhou Y, Ding J, Li X, Li Y (2023) Allicin inhibits porcine reproductive and respiratory syndrome virus infection in vitro and alleviates inflammatory responses. Viruses, 15(5):1050.","DOI":"10.3390\/v15051050"},{"issue":"10","key":"12089_CR30","doi-asserted-by":"publisher","first-page":"2517","DOI":"10.1016\/j.febslet.2006.03.071","volume":"580","author":"N Hasan","year":"2006","unstructured":"Hasan N, Yusuf N, Toossi Z, Islam N (2006) Suppression of Mycobacterium tuberculosis induced reactive oxygen species (ROS) and TNF-alpha mRNA expression in human monocytes by allicin. FEBS Lett 580(10):2517\u20132522","journal-title":"FEBS Lett"},{"issue":"3","key":"12089_CR31","doi-asserted-by":"publisher","first-page":"e14043","DOI":"10.1016\/j.heliyon.2023.e14043","volume":"9","author":"SS Choudhary","year":"2023","unstructured":"Choudhary SS, Panigrahi PN, Dhara SK, Sahoo M, Dan A, Thakur N, Jacob A, Dey S (2023) Cucumis callosus (Rottl.) Cogn. fruit extract ameliorates calcium oxalate urolithiasis in ethylene glycol induced hyperoxaluric Rat model. Heliyon 9(3):e14043","journal-title":"Heliyon"},{"issue":"4","key":"12089_CR32","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1016\/j.eururo.2017.11.001","volume":"73","author":"SA Pathan","year":"2018","unstructured":"Pathan SA, Mitra B, Cameron PA (2018) A systematic review and meta-analysis comparing the efficacy of nonsteroidal anti-inflammatory drugs, opioids, and paracetamol in the treatment of acute renal colic. Eur Urol 73(4):583\u2013595","journal-title":"Eur Urol"},{"key":"12089_CR33","doi-asserted-by":"crossref","unstructured":"Nirumand MC, Hajialyani M, Rahimi R, Farzaei MH, Zingue S, Nabavi SM, Bishayee A (2018) Dietary plants for the prevention and management of kidney stones: preclinical and clinical evidence and molecular mechanisms. Int J Mol Sci, 19(3):765.","DOI":"10.3390\/ijms19030765"},{"issue":"9","key":"12089_CR34","doi-asserted-by":"publisher","first-page":"nzz101","DOI":"10.1093\/cdn\/nzz101","volume":"3","author":"R Kanlaya","year":"2019","unstructured":"Kanlaya R, Thongboonkerd V (2019) Molecular Mechanisms of Epigallocatechin-3-Gallate for Prevention of Chronic Kidney Disease and Renal Fibrosis: Preclinical Evidence. Curr Dev Nutr 3(9):nzz101","journal-title":"Curr Dev Nutr"},{"issue":"3","key":"12089_CR35","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1515\/jtim-2016-0031","volume":"4","author":"H Bao","year":"2016","unstructured":"Bao H, Peng A (2016) The Green Tea Polyphenol(-)-epigallocatechin-3-gallate and its beneficial roles in chronic kidney disease. J Transl Int Med 4(3):99\u2013103","journal-title":"J Transl Int Med"},{"issue":"10","key":"12089_CR36","doi-asserted-by":"publisher","first-page":"2457","DOI":"10.1007\/s11255-022-03295-1","volume":"54","author":"J Zhao","year":"2022","unstructured":"Zhao J, Huang Y, Yu X (2022) Caffeine intake and the risk of incident kidney stones: a systematic review and meta-analysis. Int Urol Nephrol 54(10):2457\u20132466","journal-title":"Int Urol Nephrol"},{"issue":"3","key":"12089_CR37","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1016\/j.advnut.2023.03.002","volume":"14","author":"P Peerapen","year":"2023","unstructured":"Peerapen P, Thongboonkerd V (2023) Kidney Stone Prevention. Adv Nutr 14(3):555\u2013569","journal-title":"Adv Nutr"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-026-12089-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-026-12089-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-026-12089-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T08:28:57Z","timestamp":1781080137000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-026-12089-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,10]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["12089"],"URL":"https:\/\/doi.org\/10.1007\/s11033-026-12089-y","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,10]]},"assertion":[{"value":"22 April 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 May 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 June 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The experimental protocol was approved by the Ethics Committee of Hainan Medical University (Approval No. HYLL-2024-614). All animal experiments were performed in accordance with the relevant guidelines and regulations for the care and use of laboratory animals.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"924"}}