{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T11:04:15Z","timestamp":1784718255534,"version":"3.55.0"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Incentive Grant under the Institute of Eminence (IoE) initiative at Banaras Hindu University"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1007\/s11033-026-12304-w","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T10:03:29Z","timestamp":1784109809000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synonymous variants in IRX4 and their association with congenital heart disease: an in-silico functional assessment"],"prefix":"10.1007","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9249-2441","authenticated-orcid":false,"given":"Jyoti","family":"Maddhesiya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dharmendra","family":"Jain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1642-7733","authenticated-orcid":false,"given":"Bhagyalaxmi","family":"Mohapatra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"12304_CR1","doi-asserted-by":"publisher","first-page":"1890","DOI":"10.1016\/S0735-1097(02)01886-7","volume":"39","author":"JI Hoffman","year":"2002","unstructured":"Hoffman JI, Kaplan S (2002) The incidence of congenital heart disease. J Am Coll Cardiol 39:1890\u20131900","journal-title":"J Am Coll Cardiol"},{"key":"12304_CR2","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1007\/s11886-024-02022-9","volume":"26","author":"J Maddhesiya","year":"2024","unstructured":"Maddhesiya J, Mohapatra B (2024) Understanding the Genetic and Non-genetic Interconnections in the Aetiology of Isolated Congenital Heart Disease: An Updated Review: Part 1. Curr Cardiol Rep 26:147\u2013165","journal-title":"Curr Cardiol Rep"},{"key":"12304_CR3","doi-asserted-by":"publisher","first-page":"3644","DOI":"10.1002\/ajmg.a.62413","volume":"185","author":"R Dixit","year":"2021","unstructured":"Dixit R, Narasimhan C, Balekundri VI et al (2021) Functional analysis of novel genetic variants of <Emphasis Type=\"ItalicSmallCaps\">NKX2<\/Emphasis>-5 associated with nonsyndromic congenital heart disease. Am J Med Genet A 185:3644\u20133663. https:\/\/doi.org\/10.1002\/ajmg.a.62413","journal-title":"Am J Med Genet A"},{"key":"12304_CR4","first-page":"31","volume":"813","author":"R Dixit","year":"2019","unstructured":"Dixit R, Kumar A, Mohapatra B (2019) Implication of GATA4 synonymous variants in congenital heart disease: A comprehensive in-silico approach. Mutat Research\/Fundamental Mol Mech Mutagen 813:31\u201338","journal-title":"Mutat Research\/Fundamental Mol Mech Mutagen"},{"key":"12304_CR5","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1093\/hmg\/ddn411","volume":"18","author":"B Mohapatra","year":"2009","unstructured":"Mohapatra B, Casey B, Li H et al (2009) Identification and functional characterization of NODAL rare variants in heterotaxy and isolated cardiovascular malformations. Hum Mol Genet 18:861\u2013871","journal-title":"Hum Mol Genet"},{"key":"12304_CR6","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1161\/CIRCRESAHA.112.300853","volume":"112","author":"AC Fahed","year":"2013","unstructured":"Fahed AC, Gelb BD, Seidman JG, Seidman CE (2013) Genetics of congenital heart disease: the glass half empty. Circul Res 112:707\u2013720","journal-title":"Circul Res"},{"key":"12304_CR7","doi-asserted-by":"publisher","first-page":"4105","DOI":"10.1007\/s11033-023-08314-7","volume":"50","author":"P Giri","year":"2023","unstructured":"Giri P, Jain D, Kumar A, Mohapatra B (2023) Identification and in silico characterization of CSRP3 synonymous variants in dilated cardiomyopathy. Mol Biol Rep 50:4105\u20134117. https:\/\/doi.org\/10.1007\/s11033-023-08314-7","journal-title":"Mol Biol Rep"},{"key":"12304_CR8","doi-asserted-by":"publisher","first-page":"100987","DOI":"10.1016\/j.mgene.2021.100987","volume":"31","author":"ML Yadav","year":"2022","unstructured":"Yadav ML, Bhasker AN, Kumar A, Mohapatra B (2022) Identification and characterization of genetic variants of TGFB1 in patients with congenital heart disease. Meta Gene 31:100987","journal-title":"Meta Gene"},{"key":"12304_CR9","doi-asserted-by":"crossref","unstructured":"Cavodeassi F, Modolell J, G\u00f3mez-Skarmeta JL (2001) The Iroquois family of genes: from body building to neural patterning","DOI":"10.1242\/dev.128.15.2847"},{"key":"12304_CR10","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1007\/s00439-011-0996-7","volume":"130","author":"Z Cheng","year":"2011","unstructured":"Cheng Z, Wang J, Su D et al (2011) Two novel mutations of the IRX4 gene in patients with congenital heart disease. Hum Genet 130:657\u2013662. https:\/\/doi.org\/10.1007\/s00439-011-0996-7","journal-title":"Hum Genet"},{"key":"12304_CR11","doi-asserted-by":"publisher","first-page":"2875","DOI":"10.1002\/stem.2486","volume":"34","author":"DO Nelson","year":"2016","unstructured":"Nelson DO, Lalit PA, Biermann M et al (2016) Irx4 marks a multipotent, ventricular-specific progenitor cell. Stem Cells 34:2875\u20132888","journal-title":"Stem Cells"},{"key":"12304_CR12","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.1126\/science.283.5405.1161","volume":"283","author":"Z-Z Bao","year":"1999","unstructured":"Bao Z-Z, Bruneau BG, Seidman JG et al (1999) Regulation of Chamber-Specific Gene Expression in the Developing Heart by Irx4. Science 283:1161\u20131164. https:\/\/doi.org\/10.1126\/science.283.5405.1161","journal-title":"Science"},{"key":"12304_CR13","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1006\/dbio.1999.9548","volume":"217","author":"BG Bruneau","year":"2000","unstructured":"Bruneau BG, Bao Z-Z, Tanaka M et al (2000) Cardiac expression of the ventricle-specific homeobox gene Irx4 is modulated by Nkx2-5 and dHand. Dev Biol 217:266\u2013277","journal-title":"Dev Biol"},{"key":"12304_CR14","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1007\/s004270100147","volume":"211","author":"RJ Garriock","year":"2001","unstructured":"Garriock RJ, Vokes SA, Small EM et al (2001) Developmental expression of the Xenopus Iroquois-family homeobox genes, Irx4 and Irx5. Dev Genes Evol 211:257\u2013260. https:\/\/doi.org\/10.1007\/s004270100147","journal-title":"Dev Genes Evol"},{"key":"12304_CR15","doi-asserted-by":"publisher","first-page":"1730","DOI":"10.1128\/MCB.21.5.1730-1736.2001","volume":"21","author":"BG Bruneau","year":"2001","unstructured":"Bruneau BG, Bao Z-Z, Fatkin D et al (2001) Cardiomyopathy in Irx4 -Deficient Mice Is Preceded by Abnormal Ventricular Gene Expression. Mol Cell Biol 21:1730\u20131736. https:\/\/doi.org\/10.1128\/MCB.21.5.1730-1736.2001","journal-title":"Mol Cell Biol"},{"key":"12304_CR16","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1161\/CIRCRESAHA.112.265041","volume":"110","author":"K-H Kim","year":"2012","unstructured":"Kim K-H, Rosen A, Bruneau BG et al (2012) Iroquois homeodomain transcription factors in heart development and function. Circul Res 110:1513\u20131524","journal-title":"Circul Res"},{"key":"12304_CR17","unstructured":"Liu WF (2017) Overlapping roles of Iroquois Homeobox Genes 3 and 4 in the Compaction of the Ventricular Myocardium. University of Toronto (Canada)"},{"key":"12304_CR18","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1038\/s41419-020-02908-w","volume":"11","author":"Z Jia","year":"2020","unstructured":"Jia Z, Zhang Y, Yan A et al (2020) 1, 25-dihydroxyvitamin D3 signaling-induced decreases in IRX4 inhibits NANOG-mediated cancer stem-like properties and gefitinib resistance in NSCLC cells. Cell Death Dis 11:670","journal-title":"Cell Death Dis"},{"key":"12304_CR19","doi-asserted-by":"publisher","first-page":"e1010236","DOI":"10.1371\/journal.pgen.1010236","volume":"18","author":"DM Gordon","year":"2022","unstructured":"Gordon DM, Cunningham D, Zender G et al (2022) Exome sequencing in multiplex families with left-sided cardiac defects has high yield for disease gene discovery. PLoS Genet 18:e1010236","journal-title":"PLoS Genet"},{"key":"12304_CR20","doi-asserted-by":"publisher","first-page":"777","DOI":"10.3389\/fgene.2020.00777","volume":"11","author":"J Ahn","year":"2020","unstructured":"Ahn J, Wu H, Lee K (2020) Integrative analysis revealing human heart-specific genes and consolidating heart-related phenotypes. Front Genet 11:777","journal-title":"Front Genet"},{"key":"12304_CR21","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-022-09097-0","volume":"24","author":"S-J Wei","year":"2023","unstructured":"Wei S-J, Du J-L, Wang Y-B et al (2023) Whole exome sequencing with a focus on cardiac disease-associated genes in families of sudden unexplained deaths in Yunnan, southwest of China. BMC Genomics 24:57. https:\/\/doi.org\/10.1186\/s12864-022-09097-0","journal-title":"BMC Genomics"},{"key":"12304_CR22","doi-asserted-by":"publisher","first-page":"W287","DOI":"10.1093\/nar\/gkaa331","volume":"48","author":"M Miladi","year":"2020","unstructured":"Miladi M, Raden M, Diederichs S, Backofen R (2020) MutaRNA: analysis and visualization of mutation-induced changes in RNA structure. Nucleic Acids Res 48:W287\u2013W291","journal-title":"Nucleic Acids Res"},{"key":"12304_CR23","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/1748-7188-6-3","volume":"6","author":"SH Bernhart","year":"2011","unstructured":"Bernhart SH, M\u00fcckstein U, Hofacker IL (2011) RNA Accessibility in cubic time. Algorithms Mol Biol 6:3. https:\/\/doi.org\/10.1186\/1748-7188-6-3","journal-title":"Algorithms Mol Biol"},{"key":"12304_CR24","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1093\/nar\/gks1009","volume":"41","author":"R Salari","year":"2013","unstructured":"Salari R, Kimchi-Sarfaty C, Gottesman MM, Przytycka TM (2013) Sensitive measurement of single-nucleotide polymorphism-induced changes of RNA conformation: application to disease studies. Nucleic Acids Res 41:44\u201353","journal-title":"Nucleic Acids Res"},{"key":"12304_CR25","doi-asserted-by":"publisher","first-page":"28835","DOI":"10.1074\/jbc.M103716200","volume":"276","author":"GF Wang","year":"2001","unstructured":"Wang GF, Nikovits W, Bao Z-Z, Stockdale FE (2001) Irx4 forms an inhibitory complex with the vitamin D and retinoic X receptors to regulate cardiac chamber-specific slow MyHC3Expression. J Biol Chem 276:28835\u201328841","journal-title":"J Biol Chem"},{"key":"12304_CR26","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.45952","volume":"8","author":"E Lebeuf-Taylor","year":"2019","unstructured":"Lebeuf-Taylor E, McCloskey N, Bailey SF et al (2019) The distribution of fitness effects among synonymous mutations in a gene under directional selection. Elife 8:e45952","journal-title":"Elife"},{"key":"12304_CR27","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.tig.2014.04.006","volume":"30","author":"RC Hunt","year":"2014","unstructured":"Hunt RC, Simhadri VL, Iandoli M et al (2014) Exposing synonymous mutations. Trends Genet 30:308\u2013321","journal-title":"Trends Genet"},{"key":"12304_CR28","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1093\/hmg\/ddg055","volume":"12","author":"J Duan","year":"2003","unstructured":"Duan J, Wainwright MS, Comeron JM et al (2003) Synonymous mutations in the human dopamine receptor D2 (DRD2) affect mRNA stability and synthesis of the receptor. Hum Mol Genet 12:205\u2013216","journal-title":"Hum Mol Genet"},{"key":"12304_CR29","doi-asserted-by":"publisher","first-page":"e840","DOI":"10.1002\/mgg3.840","volume":"7","author":"UK Katneni","year":"2019","unstructured":"Katneni UK, Liss A, Holcomb D et al (2019) Splicing dysregulation contributes to the pathogenicity of several F9 exonic point variants. Mol Genet Genom Med 7:e840","journal-title":"Mol Genet Genom Med"},{"key":"12304_CR30","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1038\/ng.762","volume":"43","author":"P Brest","year":"2011","unstructured":"Brest P, Lapaquette P, Souidi M et al (2011) A synonymous variant in IRGM alters a binding site for miR-196 and causes deregulation of IRGM-dependent xenophagy in Crohn\u2019s disease. Nat Genet 43:242\u2013245","journal-title":"Nat Genet"},{"key":"12304_CR31","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1186\/s13059-023-02966-1","volume":"24","author":"BC Lin","year":"2023","unstructured":"Lin BC, Katneni U, Jankowska KI et al (2023) In silico methods for predicting functional synonymous variants. Genome Biol 24:126. https:\/\/doi.org\/10.1186\/s13059-023-02966-1","journal-title":"Genome Biol"},{"key":"12304_CR32","doi-asserted-by":"publisher","DOI":"10.1111\/hae.13107","author":"N Hamasaki-Katagiri","year":"2017","unstructured":"Hamasaki-Katagiri N, Lin BC, Simon J et al (2017) The importance of mRNA structure in determining the pathogenicity of synonymous and non\u2010synonymous mutations in haemophilia. Haemophilia. https:\/\/doi.org\/10.1111\/hae.13107","journal-title":"Haemophilia"},{"key":"12304_CR33","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10489-2","volume":"10","author":"Y Sharma","year":"2019","unstructured":"Sharma Y, Miladi M, Dukare S et al (2019) A pan-cancer analysis of synonymous mutations. Nat Commun 10:2569","journal-title":"Nat Commun"},{"key":"12304_CR34","doi-asserted-by":"publisher","DOI":"10.1261\/rna.080976.126","author":"SV Gnanasundram","year":"2026","unstructured":"Gnanasundram SV, Wang L, Chen S et al (2026) Cancer-associated synonymous mutations reveal stress signal-dependent mRNA folding that selectively modulates protein function. RNA. https:\/\/doi.org\/10.1261\/rna.080976.126","journal-title":"RNA"},{"key":"12304_CR35","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-021-08131-w","volume":"21","author":"Q Li","year":"2021","unstructured":"Li Q, Li J, Yu C et al (2021) Synonymous mutations that regulate translation speed might play a non-negligible role in liver cancer development. BMC Cancer 21:388. https:\/\/doi.org\/10.1186\/s12885-021-08131-w","journal-title":"BMC Cancer"},{"key":"12304_CR36","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1038\/nrg3051","volume":"12","author":"ZE Sauna","year":"2011","unstructured":"Sauna ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous mutations to human disease. Nat Rev Genet 12:683\u2013691","journal-title":"Nat Rev Genet"},{"key":"12304_CR37","doi-asserted-by":"publisher","first-page":"1914","DOI":"10.1093\/bioinformatics\/btw086","volume":"32","author":"P Wen","year":"2016","unstructured":"Wen P, Xiao P, Xia J (2016) dbDSM: a manually curated database for deleterious synonymous mutations. Bioinformatics 32:1914\u20131916","journal-title":"Bioinformatics"},{"key":"12304_CR38","first-page":"90","volume":"36","author":"F Bayrak","year":"2008","unstructured":"Bayrak F, K\u00f6m\u00fcrc\u00fc-Bayrak E, Mutlu B et al (2008) Genetic analysis of the Irx4 gene in hypertrophic cardiomyopathy. Turk Kardiyol Dern Ars 36:90\u201395","journal-title":"Turk Kardiyol Dern Ars"},{"key":"12304_CR39","doi-asserted-by":"publisher","first-page":"1567","DOI":"10.1172\/JCI8154","volume":"104","author":"DW Benson","year":"1999","unstructured":"Benson DW, Silberbach GM, Kavanaugh-McHugh A et al (1999) Mutations in the cardiac transcription factor NKX2. 5 affect diverse cardiac developmental pathways. J Clin Investig 104:1567\u20131573","journal-title":"J Clin Investig"},{"key":"12304_CR40","doi-asserted-by":"publisher","first-page":"12120","DOI":"10.1074\/jbc.RA118.001805","volume":"293","author":"AC Bertalovitz","year":"2018","unstructured":"Bertalovitz AC, Badhey MLO, McDonald TV (2018) Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel. J Biol Chem 293:12120\u201312136","journal-title":"J Biol Chem"},{"key":"12304_CR41","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1473","volume":"41","author":"P Gaspar","year":"2013","unstructured":"Gaspar P, Moura G, Santos MA, Oliveira JL (2013) MRNA secondary structure optimization using a correlated stem\u2013loop prediction. Nucleic Acids Res 41:e73\u2013e73","journal-title":"Nucleic Acids Res"},{"key":"12304_CR42","doi-asserted-by":"publisher","first-page":"28741","DOI":"10.1074\/jbc.M110.154575","volume":"285","author":"RA Bartoszewski","year":"2010","unstructured":"Bartoszewski RA, Jablonsky M, Bartoszewska S et al (2010) A synonymous single nucleotide polymorphism in \u2206F508 CFTR alters the secondary structure of the mRNA and the expression of the mutant protein. J Biol Chem 285:28741\u201328748","journal-title":"J Biol Chem"},{"key":"12304_CR43","doi-asserted-by":"publisher","first-page":"1446","DOI":"10.1016\/j.omtn.2021.08.007","volume":"26","author":"SY Wang","year":"2021","unstructured":"Wang SY, Cheng YY, Liu SC et al (2021) A synonymous mutation in IGF-1 impacts the transcription and translation process of gene expression. Mol Therapy Nucleic Acids 26:1446\u20131465","journal-title":"Mol Therapy Nucleic Acids"},{"key":"12304_CR44","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.compbiolchem.2016.10.008","volume":"65","author":"B Khabou","year":"2016","unstructured":"Khabou B, Siala-Sahnoun O, Gargouri L et al (2016) In silico investigation of the impact of synonymous variants in ABCB4 gene on mRNA stability\/structure, splicing accuracy and codon usage: Potential contribution to PFIC3 disease. Comput Biol Chem 65:103\u2013109","journal-title":"Comput Biol Chem"},{"key":"12304_CR45","doi-asserted-by":"crossref","unstructured":"Ranjan P, Devi C, Verma N et al (2024) Whole Exome Sequencing Uncovers Key Genetic Variants in Congenital Tooth Agenesis: An Integrative Omics Approach. medRxiv 2024\u201311","DOI":"10.1101\/2024.11.26.24317461"},{"key":"12304_CR46","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1186\/s13059-018-1437-x","volume":"19","author":"SI Adamson","year":"2018","unstructured":"Adamson SI, Zhan L, Graveley BR (2018) Vex-seq: high-throughput identification of the impact of genetic variation on pre-mRNA splicing efficiency. Genome Biol 19:71. https:\/\/doi.org\/10.1186\/s13059-018-1437-x","journal-title":"Genome Biol"},{"key":"12304_CR47","doi-asserted-by":"publisher","first-page":"5493","DOI":"10.1093\/nar\/gkac270","volume":"50","author":"RJ Pengelly","year":"2022","unstructured":"Pengelly RJ, Bakhtiar D, Borovsk\u00e1 I et al (2022) Exonic splicing code and protein binding sites for calcium. Nucleic Acids Res 50:5493\u20135512","journal-title":"Nucleic Acids Res"},{"key":"12304_CR48","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-025-12466-0","volume":"27","author":"S Srinivasan","year":"2025","unstructured":"Srinivasan S, Subramanian S, Zhou H et al (2025) Synonymous and non-synonymous variants at splice junctions can disrupt splicing and are frequently linked to disease associated loss of function genes. BMC Genomics 27:99. https:\/\/doi.org\/10.1186\/s12864-025-12466-0","journal-title":"BMC Genomics"},{"key":"12304_CR49","first-page":"60","volume":"37","author":"HA \u00c7akmak","year":"2020","unstructured":"\u00c7akmak HA, Demir M (2020) MicroRNA and cardiovascular diseases. Balkan Med J 37:60","journal-title":"Balkan Med J"},{"key":"12304_CR50","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1186\/s12915-026-02534-6","volume":"24","author":"X Song","year":"2026","unstructured":"Song X, Niu L, Fan X et al (2026) Overexpression of Rtl1 via synonymous mutation silencing miRNA target site drives skeletal muscle hypertrophy and inflammation in mice. BMC Biol 24:67. https:\/\/doi.org\/10.1186\/s12915-026-02534-6","journal-title":"BMC Biol"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-026-12304-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-026-12304-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-026-12304-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T10:36:48Z","timestamp":1784716608000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-026-12304-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,15]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["12304"],"URL":"https:\/\/doi.org\/10.1007\/s11033-026-12304-w","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,15]]},"assertion":[{"value":"9 March 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 June 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 July 2026","order":5,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Update","order":6,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The graphical abstract has been included","order":7,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"On behalf of all the authors, the corresponding author states that there is no conflict of interest. All the authors have read the manuscript and approved the submission of current version of the manuscript.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The \u2018Institutional Human Ethical Committee\u2019 of Banaras Hindu University approved the study (Ethical approval No. Dean\/2010-11\/202) design under the guidelines of \u2018Clinical Development Service Agency\u2019 by Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India.","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All participants provided informed consent for both participation and publication.","order":3,"name":"Ethics","label":"Consent for publication","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All participants provided informed consent for the participation.","order":4,"name":"Ethics","label":"Informed Consent","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"1160"}}