{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T11:20:49Z","timestamp":1787743249755,"version":"build-2784847793"},"reference-count":127,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1007\/s11033-026-12613-0","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T05:47:30Z","timestamp":1787291250000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["RNA interference mediated disease resistance in vegetable crops: fundamental discovery to current trends"],"prefix":"10.1007","volume":"53","author":[{"given":"Monisha","family":"Thangavel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamta","family":"Pathak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruma","family":"Devi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Babanjeet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anusha","family":"KR","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaswant","family":"Prajapati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohinder Kaur","family":"Sidhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"12613_CR1","doi-asserted-by":"publisher","first-page":"933017","DOI":"10.3389\/fmicb.2022.933017","volume":"13","author":"P Chaudhary","year":"2022","unstructured":"Chaudhary P, Agri U, Chaudhary A, Kumar A, Kumar G (2022) Endophytes and their potential in biotic stress management and crop production. Front Microbiol 13:933017. https:\/\/doi.org\/10.3389\/fmicb.2022.933017","journal-title":"Front Microbiol"},{"key":"12613_CR2","first-page":"882","volume":"7","author":"WM Haggag","year":"2015","unstructured":"Haggag WM, Abouziena HF, Abd-El-Kreem F, El Habbasha S (2015) Agriculture biotechnology for management of multiple biotic and abiotic environmental stress in crops. J Chem Pharm Res 7:882\u2013889","journal-title":"J Chem Pharm Res"},{"key":"12613_CR3","first-page":"237","volume":"66","author":"V Choudhary","year":"2021","unstructured":"Choudhary V, Kumar KK, Sahu BH, Muthappa SK, Das ADA (2021) Agronomic innovations in biotic stress management and its combined effect with abiotic stresses in crop production. Indian J Agron 66:237\u2013257","journal-title":"Indian J Agron"},{"key":"12613_CR4","doi-asserted-by":"publisher","first-page":"206","DOI":"10.3390\/ijms20010206","volume":"20","author":"T Qi","year":"2019","unstructured":"Qi T, Guo Jia Peng H, Liu P, Kang Z, Guo J (2019) Host-induced gene silencing: A powerful strategy to control diseases of wheat and barley. Int J Mol Sci 20:206. https:\/\/doi.org\/10.3390\/ijms20010206","journal-title":"Int J Mol Sci"},{"key":"12613_CR5","doi-asserted-by":"publisher","first-page":"946","DOI":"10.3389\/fpls.2020.00946","volume":"11","author":"PR Das","year":"2020","unstructured":"Das PR, Sherif SM (2020) Application of Exogenous dsRNAs-induced RNAi in Agriculture: Challenges and Triumphs. Front Plant Sci 11:946. https:\/\/doi.org\/10.3389\/fpls.2020.00946","journal-title":"Front Plant Sci"},{"key":"12613_CR6","doi-asserted-by":"publisher","first-page":"511","DOI":"10.3390\/metabo12060511","volume":"12","author":"A Razzaq","year":"2022","unstructured":"Razzaq A, Wishart DS, Wani SH, Hameed MK, Mubin M, Saleem F (2022) Advances in Metabolomics-Driven Diagnostic Breeding and Crop Improvement. Metabolites 12:511. https:\/\/doi.org\/10.3390\/metabo12060511","journal-title":"Metabolites"},{"key":"12613_CR7","doi-asserted-by":"publisher","first-page":"1255","DOI":"10.3390\/biology10121255","volume":"10","author":"GP Saradadevi","year":"2021","unstructured":"Saradadevi GP, Das D, Mangrauthia SK, Mohapatra S, Chikkaputtaiah C, Roorkiwal M, Solanki M, Sundaram RM, Chirravuri NN, Sakhare AS, Kota S, Varshney RK, Mohannath G (2021) Genetic, epigenetic, genomic and microbial approaches to enhance salt tolerance of plants: A comprehensive review. Biology 10:1255. https:\/\/doi.org\/10.3390\/biology10121255","journal-title":"Biology"},{"key":"12613_CR8","doi-asserted-by":"publisher","first-page":"600","DOI":"10.1016\/j.plaphy.2022.11.035","volume":"194","author":"JK Bharathi","year":"2023","unstructured":"Bharathi JK, Anandan R, Benjamin LK, Muneer S, Prakash MAS (2023) Recent trends and advances of RNA interference (RNAi) to improve agricultural crops and enhance their resilience to biotic and abiotic stresses. Plant Physiol Biochem 194:600\u2013618. https:\/\/doi.org\/10.1016\/j.plaphy.2022.11.035","journal-title":"Plant Physiol Biochem"},{"key":"12613_CR9","doi-asserted-by":"publisher","unstructured":"Zhao JH, Zhang T, Liu QY, Guo HS (2021) Trans-kingdom RNAs and their fates in recipient cells: advances, utilization and perspectives. Plant Commun 2:100167. https:\/\/doi.org\/10.1016\/j.xplc.2021.100167","DOI":"10.1016\/j.xplc.2021.100167"},{"key":"12613_CR10","doi-asserted-by":"publisher","first-page":"9755","DOI":"10.3390\/ijms24119755","volume":"24","author":"S Koeppe","year":"2023","unstructured":"Koeppe S, Kawchuk L, Kalischuk M (2023) RNA interference past and future applications in plants. Int J Mol Sci 24:9755. https:\/\/doi.org\/10.3390\/ijms24119755","journal-title":"Int J Mol Sci"},{"key":"12613_CR11","doi-asserted-by":"publisher","first-page":"643657","DOI":"10.3389\/fmolb.2021.643657","volume":"8","author":"S Paturi","year":"2021","unstructured":"Paturi S, Deshmukh MV (2021) A Glimpse of Dicer Biology Through the Structural and Functional Perspective. Front Mol Biosci 8:643657. https:\/\/doi.org\/10.3389\/fmolb.2021.643657","journal-title":"Front Mol Biosci"},{"key":"12613_CR12","doi-asserted-by":"publisher","first-page":"294","DOI":"10.18782\/2320-7051.2890","volume":"5","author":"AK Meena","year":"2017","unstructured":"Meena AK (2017) RNAi, It\u2019s Mechanism and Potential use in Crop Improvement: A review. Int J Pure Appl Biosci 5:294\u2013311. https:\/\/doi.org\/10.18782\/2320-7051.2890","journal-title":"Int J Pure Appl Biosci"},{"key":"12613_CR13","doi-asserted-by":"publisher","first-page":"1712","DOI":"10.3390\/ijms17101712","volume":"17","author":"C Catalanotto","year":"2016","unstructured":"Catalanotto C, Cogoni C, Zardo G (2016) MicroRNA in control of gene expression: An overview of nuclear functions. Int J Mol Sci 17:1712. https:\/\/doi.org\/10.3390\/ijms17101712","journal-title":"Int J Mol Sci"},{"key":"12613_CR14","doi-asserted-by":"publisher","first-page":"5503","DOI":"10.1093\/nar\/gkt251","volume":"41","author":"J Winter","year":"2013","unstructured":"Winter J, Link S, Witzigmann D, Hildenbrand C, Previti C, Diederichs S (2013) Loop-miRs: Active microRNAs generated from single-stranded loop regions. Nucleic Acids Res 41:5503\u20135512. https:\/\/doi.org\/10.1093\/nar\/gkt251","journal-title":"Nucleic Acids Res"},{"key":"12613_CR15","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1038\/nature02874","volume":"431","author":"DC Baulcombe","year":"2004","unstructured":"Baulcombe DC (2004) RNA silencing in plants. Nature 431:356\u2013363. https:\/\/doi.org\/10.1038\/nature02874","journal-title":"Nature"},{"key":"12613_CR16","doi-asserted-by":"crossref","unstructured":"Li Y, Zhao D (2013) Basics of molecular biology. Berlin, Heidelberg","DOI":"10.1007\/978-3-642-34303-2_16"},{"key":"12613_CR17","doi-asserted-by":"publisher","unstructured":"Tan SC, Yiap BC (2009) DNA, RNA, and protein extraction: the past and the present. Biomed Res Int 574398. https:\/\/doi.org\/10.1155\/2009\/574398","DOI":"10.1155\/2009\/574398"},{"key":"12613_CR18","doi-asserted-by":"publisher","unstructured":"Bhawna, Jamwal M, Kumar S, Gupta AP (2023) RNA Interference for Improvement of Bioactive Compound Production in Plants. In Biosynthesis of Bioactive Compounds in Medicinal and Aromatic Plants: Manipulation by Conventional and Biotechnological Approaches, pp 119\u2013137. https:\/\/doi.org\/10.1007\/978-3-031-35221-8_6","DOI":"10.1007\/978-3-031-35221-8_6"},{"key":"12613_CR19","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.mib.2018.02.003","volume":"46","author":"Q Cai","year":"2018","unstructured":"Cai Q, He B, Kogel KH, Jin H (2018) Cross-kingdom RNA trafficking and environmental RNAi nature\u2019s blueprint for modern crop protection strategies. Curr Opin Microbiol 46:58\u201364. https:\/\/doi.org\/10.1016\/j.mib.2018.02.003","journal-title":"Curr Opin Microbiol"},{"key":"12613_CR20","first-page":"201","volume":"1","author":"PB Jadhav","year":"2010","unstructured":"Jadhav PB, Jarande SD, Bhalerao RR, Modi PK (2010) RNA interference in fruit and vegetables for crop improvement. Adv Res J Crop Improv 1:201\u2013205","journal-title":"Adv Res J Crop Improv"},{"key":"12613_CR21","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1016\/j.tplants.2006.09.010","volume":"11","author":"S Mansoor","year":"2006","unstructured":"Mansoor S, Amin I, Hussain M, Zafar Y, Briddon RW (2006) Engineering novel traits in plants through RNA interference. Trends Plant Sci 11:559\u2013565. https:\/\/doi.org\/10.1016\/j.tplants.2006.09.010","journal-title":"Trends Plant Sci"},{"key":"12613_CR22","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1080\/14620316.2021.1988729","volume":"97","author":"H Lata","year":"2022","unstructured":"Lata H, Sharma A, Chadha S, Kaur M, Kumar P (2022) RNA interference (RNAi) mechanism and application in vegetable crops. J Hortic Sci Biotechnol 97:160\u2013170. https:\/\/doi.org\/10.1080\/14620316.2021.1988729","journal-title":"J Hortic Sci Biotechnol"},{"key":"12613_CR23","doi-asserted-by":"publisher","first-page":"2952","DOI":"10.3390\/cells10112952","volume":"10","author":"M Nicolau","year":"2021","unstructured":"Nicolau M, Picault N, Moissiard G (2021) The evolutionary volte-face of transposable elements: From harmful jumping genes to major drivers of genetic innovation. Cells 10:2952. https:\/\/doi.org\/10.3390\/cells10112952","journal-title":"Cells"},{"key":"12613_CR24","doi-asserted-by":"publisher","first-page":"108473","DOI":"10.1016\/j.mrrev.2023.108473","volume":"792","author":"D Shahwar","year":"2023","unstructured":"Shahwar D, Ahn N, Kim D, Ahn W, Park Y (2023) Mutagenesis-based plant breeding approaches and genome engineering: A review focused on tomato. Mutat Res Rev Mutat Res 792:108473. https:\/\/doi.org\/10.1016\/j.mrrev.2023.108473","journal-title":"Mutat Res Rev Mutat Res"},{"key":"12613_CR25","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1104\/pp.19.00570","volume":"182","author":"A Dalakouras","year":"2020","unstructured":"Dalakouras A, Wassenegger M, Dadami E, Ganopoulos I, Pappas ML, Papadopoulou K (2020) Genetically modified organism-free RNA interference: Exogenous application of RNA molecules in plants. Plant Physiol 182:38\u201350. https:\/\/doi.org\/10.1104\/pp.19.00570","journal-title":"Plant Physiol"},{"key":"12613_CR26","doi-asserted-by":"crossref","unstructured":"Shahid M, Zaidi A, Khan MS, Rizvi A, Saif S, Ahmed B (2017) Recent advances in management strategies of vegetable diseases. In: Zaidi A, Khan M (eds) Microbial strategies for vegetable production. Springer, Cham, pp 197\u2013226","DOI":"10.1007\/978-3-319-54401-4_9"},{"key":"12613_CR27","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1094\/MPMI-20-6-0717","volume":"20","author":"K Bonfim","year":"2007","unstructured":"Bonfim K, Faria JC, Nogueira EO, Mendes EA, Aragao FJ (2007) RNAi-mediated resistance to Bean golden mosaic virus in genetically engineered common bean (Phaseolus vulgaris). Mol Plant Microbe Interact 20:717\u2013726. https:\/\/doi.org\/10.1094\/MPMI-20-6-0717","journal-title":"Mol Plant Microbe Interact"},{"key":"12613_CR28","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1007\/s11105-020-01205-2","volume":"38","author":"N Singh","year":"2020","unstructured":"Singh N, Mukherjee SK, Rajam MV (2020) Silencing of the Ornithine Decarboxylase Gene of Fusarium oxysporum f. sp. lycopersici by Host-Induced RNAi Confers Resistance to Fusarium Wilt in Tomato. Plant Mol Biol Rep 38:419\u2013429. https:\/\/doi.org\/10.1007\/s11105-020-01205-2","journal-title":"Plant Mol Biol Rep"},{"key":"12613_CR29","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1101\/2020.01.03.893677","volume":"104","author":"BK Mahto","year":"2020","unstructured":"Mahto BK, Singh A, Pareek M, Rajam MV, Dhar-Ray S, Reddy PM (2020) Host-induced silencing of the Colletotrichum gloeosporioides conidial morphology 1 gene (CgCOM1) confers resistance against Anthracnose disease in chilli and tomato. Plant Mol Biol 104:381\u2013395. https:\/\/doi.org\/10.1101\/2020.01.03.893677","journal-title":"Plant Mol Biol"},{"key":"12613_CR30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/nplants.2016.207","volume":"3","author":"N Mitter","year":"2017","unstructured":"Mitter N, Worrall EA, Robinson KE, Li P, Jain RG, Taochy C, Fletcher SJ, Carroll BJ, Lu GQ, Xu ZP (2017) Clay nanosheets for topical delivery of RNAi for sustained protection against plant viruses. Nat Plants 3:1\u201310. https:\/\/doi.org\/10.1038\/nplants.2016.207","journal-title":"Nat Plants"},{"key":"12613_CR31","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1111\/mpp.12572","volume":"19","author":"A Kaldis","year":"2018","unstructured":"Kaldis A, Berbati M, Melita O, Reppa C, Holeva M, Otten P, Voloudakis A (2018) Exogenously applied dsRNA molecules deriving from the Zucchini yellow mosaic virus (ZYMV) genome move systemically and protect cucurbits against ZYMV. Mol Plant Pathol 19:883\u2013895. https:\/\/doi.org\/10.1111\/mpp.12572","journal-title":"Mol Plant Pathol"},{"key":"12613_CR32","doi-asserted-by":"publisher","first-page":"2187","DOI":"10.1111\/jipb.13353","volume":"64","author":"J Nino-Sanchez","year":"2022","unstructured":"Nino-Sanchez J, Sambasivam PT, Sawyer A, Hamby R, Chen A, Czislowski E, Jin H (2022) BioClay\u2122 prolongs RNA interference\u2010mediated crop protection against Botrytis cinerea. J Integr Plant Biol 64:2187\u20132198. https:\/\/doi.org\/10.1111\/jipb.13353","journal-title":"J Integr Plant Biol"},{"key":"12613_CR33","doi-asserted-by":"publisher","first-page":"3183","DOI":"10.1002\/ps.6949","volume":"78","author":"S Sundaresha","year":"2022","unstructured":"Sundaresha S, Sharma S, Bairwa A, Tomar M, Kumar R, Bhardwaj V, Chakrabarti SK (2022) Spraying of dsRNA molecules derived from Phytophthora infestans, along with nanoclay carriers as a proof of concept for developing novel protection strategy for potato late blight. Pest Manag Sci 78:3183\u20133192. https:\/\/doi.org\/10.1002\/ps.6949","journal-title":"Pest Manag Sci"},{"key":"12613_CR34","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.virol.2007.09.031","volume":"371","author":"A Carbonell","year":"2008","unstructured":"Carbonell A, de Alba AEM, Flores R, Gago S (2008) Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families. Virology 371:44\u201353. https:\/\/doi.org\/10.1016\/j.virol.2007.09.031","journal-title":"Virology"},{"key":"12613_CR35","doi-asserted-by":"publisher","first-page":"83","DOI":"10.5511\/plantbiotechnology.19.1227a","volume":"37","author":"H Kim","year":"2020","unstructured":"Kim H, Onodera Y, Masuta C (2020) Application of cucumber mosaic virus to efficient induction and long-term maintenance of virus-induced gene silencing in spinach. Plant Biotechnol 37:83\u201388. https:\/\/doi.org\/10.5511\/plantbiotechnology.19.1227a","journal-title":"Plant Biotechnol"},{"key":"12613_CR36","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.virusres.2012.12.008","volume":"172","author":"T Van Vu","year":"2013","unstructured":"Van Vu T, Choudhury NR, Mukherjee SK (2013) Transgenic tomato plants expressing artificial microRNAs for silencing the pre-coat and coat proteins of a begomovirus, Tomato leaf curl New Delhi virus, show tolerance to virus infection. Virus Res 172:35\u201345. https:\/\/doi.org\/10.1016\/j.virusres.2012.12.008","journal-title":"Virus Res"},{"key":"12613_CR37","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1186\/s13007-020-0560-3","volume":"16","author":"M Liu","year":"2020","unstructured":"Liu M, Liang Z, Aranda MA, Hong N, Liu L, Kang B, Gu Q (2020) A cucumber green mottle mosaic virus vector for virus-induced gene silencing in cucurbit plants. Plant Methods 16:9. https:\/\/doi.org\/10.1186\/s13007-020-0560-3","journal-title":"Plant Methods"},{"key":"12613_CR38","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1111\/tpj.15164","volume":"106","author":"M Uranga","year":"2021","unstructured":"Uranga M, Aragones V, Selma S, Vazquez-Vilar M, Orzaez D, Daros JA (2021) Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector. Plant J 106:555\u2013565. https:\/\/doi.org\/10.1111\/tpj.15164","journal-title":"Plant J"},{"key":"12613_CR39","doi-asserted-by":"publisher","unstructured":"Napoli C, Lemieux C, Jorgensen R (1990) lntroduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes \u00cdn trans. Plant Cell 2:279-289. https:\/\/doi.org\/10.1105\/tpc.2.4.279","DOI":"10.1105\/tpc.2.4.279"},{"key":"12613_CR40","first-page":"9322","volume":"10","author":"TL Dheemanth","year":"2017","unstructured":"Dheemanth TL, Ramachandra RK, Chavan M, Prakash BG (2017) RNA interference in crop improvement. Trends Biosci 10:9322\u20139335","journal-title":"Trends Biosci"},{"key":"12613_CR41","doi-asserted-by":"publisher","first-page":"1","DOI":"10.22271\/ed.book.2864","volume":"3","author":"D Anavarali","year":"2014","unstructured":"Anavarali D, Waseem S, Acharya S (2014) RNA interference: A new technology for crop improvement. Int J Electron Commun 3:1\u201324. https:\/\/doi.org\/10.22271\/ed.book.2864","journal-title":"Int J Electron Commun"},{"key":"12613_CR42","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.bbagrm.2015.08.007","volume":"1859","author":"H Kobayashi","year":"2016","unstructured":"Kobayashi H, Tomari Y (2016) RISC assembly: Coordination between small RNAs and Argonaute proteins. Biochim Biophys Acta Gene Regul Mech 1859:71\u201381. https:\/\/doi.org\/10.1016\/j.bbagrm.2015.08.007","journal-title":"Biochim Biophys Acta Gene Regul Mech"},{"key":"12613_CR43","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1016\/j.cell.2005.08.044","volume":"123","author":"C Matranga","year":"2005","unstructured":"Matranga C, Tomari Y, Shin C, Bartel DP, Zamore PD (2005) Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes. Cell 123:607\u2013620. https:\/\/doi.org\/10.1016\/j.cell.2005.08.044","journal-title":"Cell"},{"key":"12613_CR44","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1128\/mmbr.67.4.657-685.2003","volume":"67","author":"N Agrawal","year":"2003","unstructured":"Agrawal N, Dasaradhi PVN, Mohmmed A, Malhotra P, Bhatnagar RK, Mukherjee SK (2003) RNA Interference: Biology, Mechanism and Applications. Microbiol Mol Biol Rev 67:657\u2013685. https:\/\/doi.org\/10.1128\/mmbr.67.4.657-685.2003","journal-title":"Microbiol Mol Biol Rev"},{"key":"12613_CR45","doi-asserted-by":"publisher","first-page":"313","DOI":"10.3389\/fimmu.2017.00313","volume":"8","author":"C Peteranderl","year":"2017","unstructured":"Peteranderl C, Herold S (2017) The impact of the interferon\/TNF-related apoptosis-inducing ligand signaling axis on disease progression in respiratory viral infection and beyond. Front Immunol 8:313. https:\/\/doi.org\/10.3389\/fimmu.2017.00313","journal-title":"Front Immunol"},{"key":"12613_CR46","doi-asserted-by":"publisher","first-page":"2303","DOI":"10.5897\/AJB12.2791","volume":"12","author":"VK Sharma","year":"2013","unstructured":"Sharma VK, Sanghera GS, Kashyap PL, Sharma BB, Chandel C (2013) RNA interference: A novel tool for plant disease management. Afr J Biotechnol 12:2303\u20132312. https:\/\/doi.org\/10.5897\/AJB12.2791","journal-title":"Afr J Biotechnol"},{"key":"12613_CR47","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1038\/nrg3683","volume":"15","author":"MA Matzke","year":"2014","unstructured":"Matzke MA, Mosher RA (2014) RNA-directed DNA methylation: an epigenetic pathway of increasing complexity. Nat Rev Genet 15:394\u2013408. https:\/\/doi.org\/10.1038\/nrg3683","journal-title":"Nat Rev Genet"},{"key":"12613_CR48","doi-asserted-by":"publisher","first-page":"990","DOI":"10.1104\/pp.112.200279","volume":"160","author":"L Wu","year":"2012","unstructured":"Wu L, Mao L, Qi Y (2012) Roles of dicer-like and argonaute proteins in TAS-derived small interfering RNA-triggered DNA methylation. Plant Physiol 160:990\u2013999. https:\/\/doi.org\/10.1104\/pp.112.200279","journal-title":"Plant Physiol"},{"key":"12613_CR49","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1016\/j.sbi.2005.05.006","volume":"15","author":"W Filipowicz","year":"2005","unstructured":"Filipowicz W, Jaskiewicz L, Kolb FA, Pillai RS (2005) Post-transcriptional gene silencing by siRNAs and miRNAs. Curr Opin Struct Biol 15:331\u2013341. https:\/\/doi.org\/10.1016\/j.sbi.2005.05.006","journal-title":"Curr Opin Struct Biol"},{"key":"12613_CR50","doi-asserted-by":"publisher","first-page":"1829","DOI":"10.1371\/journal.pone.0001829","volume":"3","author":"N Warthmann","year":"2008","unstructured":"Warthmann N, Chen H, Ossowski S, Weigel D, Herve P (2008) Highly specific gene silencing by artificial miRNAs in rice. PLoS ONE 3:1829. https:\/\/doi.org\/10.1371\/journal.pone.0001829","journal-title":"PLoS ONE"},{"key":"12613_CR51","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1038\/nsmb.2230","volume":"19","author":"SW Chi","year":"2012","unstructured":"Chi SW, Hannon GJ, Darnell RB (2012) An alternative mode of microRNA target recognition. Nat Struct Mol Biol 19:321\u2013327. https:\/\/doi.org\/10.1038\/nsmb.2230","journal-title":"Nat Struct Mol Biol"},{"key":"12613_CR52","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1038\/nrm3838","volume":"15","author":"M Ha","year":"2014","unstructured":"Ha M, Kim VN (2014) Regulation of microRNA biogenesis. Nat Rev Mol Cell Biol 15:509\u2013524. https:\/\/doi.org\/10.1038\/nrm3838","journal-title":"Nat Rev Mol Cell Biol"},{"key":"12613_CR53","doi-asserted-by":"publisher","unstructured":"Jiang S, Yan W (2016) Current View of microRNA Processing. Sign Transduct Insights 5:STI-S12 https:\/\/doi.org\/10.4137\/sti.s12317","DOI":"10.4137\/sti.s12317"},{"key":"12613_CR54","doi-asserted-by":"publisher","first-page":"2859","DOI":"10.1007\/s00018-011-0726-2","volume":"68","author":"J Starega-Roslan","year":"2011","unstructured":"Starega-Roslan J, Koscianska E, Kozlowski P, Krzyzosiak WJ (2011) The role of the precursor structure in the biogenesis of microRNA. Cell Mol Life Sci 68:2859\u20132871. https:\/\/doi.org\/10.1007\/s00018-011-0726-2","journal-title":"Cell Mol Life Sci"},{"key":"12613_CR55","doi-asserted-by":"publisher","first-page":"844149","DOI":"10.3389\/fpls.2022.844149","volume":"13","author":"L Zhang","year":"2022","unstructured":"Zhang L, Xiang Y, Chen S, Shi M, Jiang X, He Z, Gao S (2022) Mechanisms of MicroRNA Biogenesis and Stability Control in Plants. Front Plant Sci 13:844149. https:\/\/doi.org\/10.3389\/fpls.2022.844149","journal-title":"Front Plant Sci"},{"key":"12613_CR56","doi-asserted-by":"publisher","first-page":"9418","DOI":"10.1093\/nar\/gkv937","volume":"43","author":"JH Park","year":"2015","unstructured":"Park JH, Shin C (2015) Slicer-independent mechanism drives small-RNA strand separation during human RISC assembly. Nucleic Acids Res 43:9418\u20139433. https:\/\/doi.org\/10.1093\/nar\/gkv937","journal-title":"Nucleic Acids Res"},{"key":"12613_CR57","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1007\/s00114-011-0798-8","volume":"98","author":"UB Jagtap","year":"2011","unstructured":"Jagtap UB, Gurav RG, Bapat VA (2011) Role of RNA interference in plant improvement. Naturwissenschaften 98:473\u2013492. https:\/\/doi.org\/10.1007\/s00114-011-0798-8","journal-title":"Naturwissenschaften"},{"key":"12613_CR58","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1038\/s41580-020-00315-9","volume":"22","author":"L Statello","year":"2021","unstructured":"Statello L, Guo CJ, Chen LL, Huarte M (2021) Gene regulation by long non-coding RNAs and its biological functions. Nat Rev Mol Cell Biol 22:96\u2013118. https:\/\/doi.org\/10.1038\/s41580-020-00315-9","journal-title":"Nat Rev Mol Cell Biol"},{"key":"12613_CR59","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1038\/s41580-022-00566-8","volume":"24","author":"JS Mattick","year":"2023","unstructured":"Mattick JS, Amaral PP, Carninci P, Carpenter S, Chang HY, Chen LL, Wu M (2023) Long non-coding RNAs: definitions, functions, challenges and recommendations. Nat Rev Mol Cell Biol 24:430\u2013447. https:\/\/doi.org\/10.1038\/s41580-022-00566-8","journal-title":"Nat Rev Mol Cell Biol"},{"key":"12613_CR60","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1146\/annurev-arplant-093020-035446","volume":"72","author":"AT Wierzbicki","year":"2021","unstructured":"Wierzbicki AT, Blevins T, Swiezewski S (2021) Long noncoding RNAs in plants. Annu Rev Plant Biol 72:245\u2013271. https:\/\/doi.org\/10.1146\/annurev-arplant-093020-035446","journal-title":"Annu Rev Plant Biol"},{"key":"12613_CR61","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/j.pbi.2015.08.003","volume":"27","author":"JA Chekanova","year":"2015","unstructured":"Chekanova JA (2015) Long non-coding RNAs and their functions in plants. Curr Opin Plant Biol 27:207\u2013216. https:\/\/doi.org\/10.1016\/j.pbi.2015.08.003","journal-title":"Curr Opin Plant Biol"},{"key":"12613_CR62","doi-asserted-by":"publisher","first-page":"1905","DOI":"10.1016\/j.biochi.2011.07.032","volume":"93","author":"S Rother","year":"2011","unstructured":"Rother S, Meister G (2011) Small RNAs derived from longer non-coding RNAs. Biochimie 93:1905\u20131915. https:\/\/doi.org\/10.1016\/j.biochi.2011.07.032","journal-title":"Biochimie"},{"key":"12613_CR63","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1186\/s43042-024-00553-y","volume":"25","author":"TP George","year":"2024","unstructured":"George TP, Subramanian S, Supriya MH (2024) A brief review of noncoding RNA. Egypt J Med Hum Genet 25:98. https:\/\/doi.org\/10.1186\/s43042-024-00553-y","journal-title":"Egypt J Med Hum Genet"},{"key":"12613_CR64","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/S1360-1385(01)01989-6","volume":"6","author":"PM Waterhouse","year":"2001","unstructured":"Waterhouse PM, Wang MB, Finnegan EJ (2001) Role of short RNAs in gene silencing. Trends Plant Sci 6:297\u2013301","journal-title":"Trends Plant Sci"},{"key":"12613_CR65","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.pbi.2011.07.011","volume":"14","author":"CA Brosnan","year":"2011","unstructured":"Brosnan CA, Voinnet O (2011) Cell-to-cell and long-distance siRNA movement in plants: Mechanisms and biological implications. Curr Opin Plant Biol 14:58\u2013587. https:\/\/doi.org\/10.1016\/j.pbi.2011.07.011","journal-title":"Curr Opin Plant Biol"},{"key":"12613_CR66","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1111\/jipb.12423","volume":"58","author":"G Mermigka","year":"2016","unstructured":"Mermigka G, Verret F, Kalantidis K (2016) RNA silencing movement in plants. J Integr Plant Biol 58:328\u2013342. https:\/\/doi.org\/10.1111\/jipb.12423","journal-title":"J Integr Plant Biol"},{"key":"12613_CR67","doi-asserted-by":"publisher","unstructured":"Cagliari D, Avila dos Santos E, Dias N, Smagghe G, Zotti M (2019) Nontransformative Strategies for RNAi in Crop Protection. In: Aditi S, Khan MV (eds) Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies. IntechOpen, London, United Kingdom, pp 41-57. https:\/\/doi.org\/10.5772\/intechopen.80874","DOI":"10.5772\/intechopen.80874"},{"key":"12613_CR68","doi-asserted-by":"publisher","first-page":"39","DOI":"10.3389\/fpls.2012.00039","volume":"3","author":"VM Haroldsen","year":"2012","unstructured":"Haroldsen VM, Szczerba MW, Aktas H, Lopez-Baltazar J, Odias MJ, Chi-Ham CL, Labavitch JM, Bennett AB, Powell ALT (2012) Mobility of transgenic nucleic acids and proteins within grafted rootstocks for agricultural improvement. Front Plant Sci 3:39. https:\/\/doi.org\/10.3389\/fpls.2012.00039","journal-title":"Front Plant Sci"},{"key":"12613_CR69","doi-asserted-by":"publisher","first-page":"3553","DOI":"10.1038\/emboj.2011.274","volume":"30","author":"CW Melnyk","year":"2011","unstructured":"Melnyk CW, Molnar A, Baulcombe DC (2011) Intercellular and systemic movement of RNA silencing signals. EMBO J 30:3553\u20133563. https:\/\/doi.org\/10.1038\/emboj.2011.274","journal-title":"EMBO J"},{"key":"12613_CR70","doi-asserted-by":"publisher","first-page":"13","DOI":"10.32607\/20758251-2019-11-4-13-21","volume":"11","author":"SY Morozov","year":"2019","unstructured":"Morozov SY, Solovyev AG, Kalinina NO, Taliansky ME (2019) Double-stranded RNAs in plant protection against pathogenic organisms and viruses in agriculture. Acta Nat 11:13\u201321. https:\/\/doi.org\/10.32607\/20758251-2019-11-4-13-21","journal-title":"Acta Nat"},{"key":"12613_CR71","doi-asserted-by":"publisher","first-page":"2700","DOI":"10.3390\/molecules28062700","volume":"28","author":"NS Mat Jalaluddin","year":"2023","unstructured":"Mat Jalaluddin NS, Asem M, Harikrishna JA, Ahmad Fuaad AAH (2023) Recent Progress on Nanocarriers for Topical-Mediated RNAi Strategies for Crop Protection: A Review. Molecules 28:2700. https:\/\/doi.org\/10.3390\/molecules28062700","journal-title":"Molecules"},{"key":"12613_CR72","doi-asserted-by":"publisher","first-page":"e1005901","DOI":"10.1371\/journal.ppat.1005901","volume":"12","author":"A Koch","year":"2016","unstructured":"Koch A, Biedenkopf D, Furch A, Weber L, Rossbach O, Abdellatef E, Linicus L, Johannsmeier J, Jelonek L, Goesmann A, Cardoza V, McMillan J, Mentzel T, Kogel KH (2016) An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery. PLoS Pathog 12:e1005901. https:\/\/doi.org\/10.1371\/journal.ppat.1005901","journal-title":"PLoS Pathog"},{"key":"12613_CR73","doi-asserted-by":"publisher","first-page":"451","DOI":"10.3389\/fpls.2020.00451","volume":"11","author":"O Christiaens","year":"2020","unstructured":"Christiaens O, Whyard S, Velez AM, Smagghe G (2020) Double-Stranded RNA Technology to Control Insect Pests: Current Status and Challenges. Front Plant Sci 11:451. https:\/\/doi.org\/10.3389\/fpls.2020.00451","journal-title":"Front Plant Sci"},{"key":"12613_CR74","doi-asserted-by":"publisher","first-page":"1132","DOI":"10.3390\/agronomy10081132","volume":"10","author":"P Sarkar","year":"2020","unstructured":"Sarkar P, Ghanim M (2020) Unravelling the pathogenesis and molecular interactions of liberibacter phytopathogens with their psyllid vectors. Agron J 10:1132. https:\/\/doi.org\/10.3390\/agronomy10081132","journal-title":"Agron J"},{"key":"12613_CR75","doi-asserted-by":"publisher","first-page":"714116","DOI":"10.3389\/fpls.2021.714116","volume":"12","author":"A Szekacs","year":"2021","unstructured":"Szekacs A, Ammour AS, Mendelsohn ML (2021) Editorial: RNAi Based Pesticides. Front Plant Sci 12:714116. https:\/\/doi.org\/10.3389\/fpls.2021.714116","journal-title":"Front Plant Sci"},{"key":"12613_CR76","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/S1369-5266(99)80022-3","volume":"2","author":"DC Baulcombe","year":"1999","unstructured":"Baulcombe DC (1999) Fast forward genetics based on virus-induced gene silencing. Curr Opin Plant Biol 2:109\u2013113. https:\/\/doi.org\/10.1016\/S1369-5266(99)80022-3","journal-title":"Curr Opin Plant Biol"},{"key":"12613_CR77","doi-asserted-by":"crossref","unstructured":"Mahgoub Azooz M, Ahmad P (2015) Legumes under Environmental Stress. Yield, Improvement and Adaptations","DOI":"10.1002\/9781118917091"},{"key":"12613_CR78","doi-asserted-by":"publisher","first-page":"42","DOI":"10.21203\/rs.2.21427\/v1","volume":"252","author":"Z Xiao","year":"2020","unstructured":"Xiao Z, Xing M, Liu X, Fang Z, Yang L, Zhang Y, Wang Y, Zhuang M, Lv H (2020) An efficient virus-induced gene silencing (VIGS) system for functional genomics in Brassicas using a cabbage leaf curl virus (CaLCuV)-based vector. Planta 252:42. https:\/\/doi.org\/10.21203\/rs.2.21427\/v1","journal-title":"Planta"},{"key":"12613_CR79","doi-asserted-by":"publisher","first-page":"5312","DOI":"10.3390\/ijms23105312","volume":"23","author":"S Akbar","year":"2022","unstructured":"Akbar S, Wei Y, Zhang MQ (2022) RNA Interference: Promising Approach to Combat Plant Viruses. Int J Mol Sci 23:5312. https:\/\/doi.org\/10.3390\/ijms23105312","journal-title":"Int J Mol Sci"},{"key":"12613_CR80","doi-asserted-by":"publisher","first-page":"919","DOI":"10.1093\/jxb\/eru447","volume":"66","author":"X Ma","year":"2015","unstructured":"Ma X, Nicole MC, Meteignier LV, Hong N, Wang G, Moffett P (2015) Different roles for RNA silencing and RNA processing components in virus recovery and virus-induced gene silencing in plants. J Exp Bot 66:919\u2013932. https:\/\/doi.org\/10.1093\/jxb\/eru447","journal-title":"J Exp Bot"},{"key":"12613_CR81","doi-asserted-by":"publisher","first-page":"934","DOI":"10.3390\/horticulturae","volume":"9","author":"Z Wang","year":"2023","unstructured":"Wang Z, Cao S, Xu X, He Y, Shou W, Munaiz ED, Yu C, Shen J (2023) Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables. Horticulturae 9:934. https:\/\/doi.org\/10.3390\/horticulturae","journal-title":"Horticulturae"},{"key":"12613_CR82","doi-asserted-by":"publisher","first-page":"323","DOI":"10.3389\/fpls.2014.00323","volume":"5","author":"V Ramegowda","year":"2014","unstructured":"Ramegowda V, Mysore KS, Senthil-Kumar M (2014) Virus-induced gene silencing is a versatile tool for unraveling the functional relevance of multiple abiotic-stress-responsive genes in crop plants. Front Plant Sci 5:323. https:\/\/doi.org\/10.3389\/fpls.2014.00323","journal-title":"Front Plant Sci"},{"key":"12613_CR83","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1111\/j.1365-313X.2004.02158.x","volume":"39","author":"TM Burch-Smith","year":"2004","unstructured":"Burch-Smith TM, Anderson JC, Martin GB, Dinesh-Kumar SP (2004) Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J 39:734\u2013746. https:\/\/doi.org\/10.1111\/j.1365-313X.2004.02158.x","journal-title":"Plant J"},{"key":"12613_CR84","doi-asserted-by":"publisher","first-page":"579","DOI":"10.2135\/cropsci2017.06.0355","volume":"58","author":"TLP Souza","year":"2018","unstructured":"Souza TLP, Faria JC, Arag\u00e3o FJ, Del Peloso MJ, Faria LC, Wendland A, Melo LC (2018) Agronomic performance and yield stability of the RNA interference-based Bean golden mosaic virus\u2010resistant common bean. Crop Sci 58:579\u2013591. https:\/\/doi.org\/10.2135\/cropsci2017.06.0355","journal-title":"Crop Sci"},{"key":"12613_CR85","doi-asserted-by":"publisher","first-page":"265","DOI":"10.3929\/ethz-b-000021361","volume":"70","author":"H Vanderschuren","year":"2009","unstructured":"Vanderschuren H, Alder A, Zhang P, Gruissem W (2009) Dose-dependent RNAi-mediated geminivirus resistance in the tropical root crop cassava. Plant Mol Biol 70:265\u2013272. https:\/\/doi.org\/10.3929\/ethz-b-000021361","journal-title":"Plant Mol Biol"},{"key":"12613_CR86","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1023\/B:MOLB.0000038006.32812.52","volume":"14","author":"A Missiou","year":"2004","unstructured":"Missiou A, Kalantidis K, Boutla A, Tzortzakaki S, Tabler M, Tsagris M (2004) Generation of transgenic potato plants highly resistant to potato virus Y (PVY) through RNA silencing. Mol Breed 14:185\u2013197. https:\/\/doi.org\/10.1023\/B:MOLB.0000038006.32812.52","journal-title":"Mol Breed"},{"key":"12613_CR87","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1007\/s11240-022-02426-x","volume":"152","author":"S Sharma","year":"2023","unstructured":"Sharma S, Kumar A, Kumari N, Walia A (2023) RNAi as a tool to enhance crop yield and biotic stress management in the plants. Plant Cell Tissue Organ Cult 152:437\u2013454. https:\/\/doi.org\/10.1007\/s11240-022-02426-x","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"12613_CR88","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1007\/s11032-006-9030-5","volume":"18","author":"BL Lennefors","year":"2006","unstructured":"Lennefors BL, Savenkov EI, Bensefelt J, Wremerth-Weich E, van Roggen P, Tuvesson S, Gielen J (2006) dsRNA-mediated resistance to Beet Necrotic Yellow Vein Virus infections in sugar beet (Beta vulgaris L. ssp. vulgaris). Mol Breed 18:313\u2013325. https:\/\/doi.org\/10.1007\/s11032-006-9030-5","journal-title":"Mol Breed"},{"key":"12613_CR89","doi-asserted-by":"publisher","first-page":"6053147","DOI":"10.1155\/2016\/6053147","volume":"2016","author":"O Taha","year":"2016","unstructured":"Taha O, Farouk I, Abdallah A, Abdallah NA (2016) Use of posttranscription gene silencing in squash to induce resistance against the Egyptian isolate of the Squash leaf curl virus. Int J Genomics 2016:6053147. https:\/\/doi.org\/10.1155\/2016\/6053147","journal-title":"Int J Genomics"},{"key":"12613_CR90","unstructured":"Ramesh SV (2007) Suppression of Tomato leaf curl virus (ToLCV) through RNA interference (RNAi) (Doctoral dissertation, Ph. D. Thesis, IARI, New Delhi, India)"},{"key":"12613_CR91","doi-asserted-by":"publisher","first-page":"e0186786","DOI":"10.1371\/journal.pone.0186786","volume":"12","author":"S Kumar","year":"2017","unstructured":"Kumar S, Tanti B, Patil BL, Mukherjee SK, Sahoo L (2017) RNAi-derived transgenic resistance to Mungbean yellow mosaic India virus in cowpea. PLoS ONE 12:e0186786. https:\/\/doi.org\/10.1371\/journal.pone.0186786","journal-title":"PLoS ONE"},{"key":"12613_CR92","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1111\/j.1364-3703.2008.00480.x","volume":"9","author":"JF Kreuze","year":"2008","unstructured":"Kreuze JF, Klein IS, Lazaro MU, Chuquiyuri WJC, Morgan GL, Mejia PGC, Valkonen JP (2008) RNA silencing-mediated resistance to a crinivirus (Closteroviridae) in cultivated sweetpotato (Ipomoea batatas L.) and development of sweetpotato virus disease following co\u2010infection with a potyvirus. Mol Plant Pat 9:589\u2013598. https:\/\/doi.org\/10.1111\/j.1364-3703.2008.00480.x","journal-title":"Mol Plant Pat"},{"key":"12613_CR93","doi-asserted-by":"publisher","first-page":"2052","DOI":"10.3389\/fpls.2016.02052","volume":"7","author":"G Beyene","year":"2017","unstructured":"Beyene G, Chauhan RD, Ilyas M, Wagaba H, Fauquet CM, Miano D, Taylor NJ (2017) A virus-derived stacked RNAi construct confers robust resistance to cassava brown streak disease. Front Plant Sci 7:2052. https:\/\/doi.org\/10.3389\/fpls.2016.02052","journal-title":"Front Plant Sci"},{"key":"12613_CR94","doi-asserted-by":"publisher","first-page":"e00383","DOI":"10.1016\/j.btre.2019.e00383","volume":"24","author":"HA Walsh","year":"2019","unstructured":"Walsh HA, Vanderschuren H, Taylor S, Rey MEC (2019) RNA silencing of South African cassava mosaic virus in transgenic cassava expressing AC1\/AC4 hp-RNA induces tolerance. Biotechnol Rep 24:e00383. https:\/\/doi.org\/10.1016\/j.btre.2019.e00383","journal-title":"Biotechnol Rep"},{"key":"12613_CR95","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1111\/j.1364-3703.2010.00700.x","volume":"12","author":"JS Yadav","year":"2011","unstructured":"Yadav JS, Ogwok E, Wagaba H, Patil BL, Bagewadi B, Alicai T, Fauquet CM (2011) RNAi-mediated resistance to Cassava brown streak Uganda virus in transgenic cassava. Mol Plant Pat 12:677\u2013687. https:\/\/doi.org\/10.1111\/j.1364-3703.2010.00700.x","journal-title":"Mol Plant Pat"},{"key":"12613_CR96","doi-asserted-by":"publisher","unstructured":"Jin FM, Song J, Xue J, Sun HB, Zhang Y, Wang S, Wang YH (2020) Successful generation of anti-ToCV and TYLCV transgenic tomato plants by RNAi. Biol Plant 64 :490-496. https:\/\/doi.org\/10.32615\/bp.2020.069","DOI":"10.32615\/bp.2020.069"},{"key":"12613_CR97","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1094\/MPMI-08-15-0181-R","volume":"29","author":"A Fuentes","year":"2016","unstructured":"Fuentes A, Carlos N, Ruiz Y, Callard D, S\u00e1nchez Y, Ochagav\u00eda ME, Pooggin MM (2016) Field trial and molecular characterization of RNAi-transgenic tomato plants that exhibit resistance to tomato yellow leaf curl geminivirus. Mol Plant-Microbe Interact 29:197\u2013209. https:\/\/doi.org\/10.1094\/MPMI-08-15-0181-R","journal-title":"Mol Plant-Microbe Interact"},{"key":"12613_CR98","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1016\/j.funbio.2017.05.005","volume":"121","author":"M Pareek","year":"2017","unstructured":"Pareek M, Rajam MV (2017) RNAi-mediated silencing of MAP kinase signalling genes (Fmk1, Hog1 and Pbs2) in Fusarium oxysporum reduces pathogenesis on tomato plants. Fungal Biol 121:775\u2013784. https:\/\/doi.org\/10.1016\/j.funbio.2017.05.005","journal-title":"Fungal Biol"},{"key":"12613_CR99","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1111\/j.1469-8137.2011.04024.x","volume":"193","author":"L Eschen-Lippold","year":"2012","unstructured":"Eschen-Lippold L, Landgraf R, Smolka U, Schulze S, Heilmann M, Heilmann I, Rosahl S (2012) Activation of defense against Phytophthora infestans in potato by down\u2010regulation of syntaxin gene expression. New Phytol 193:985\u2013996. https:\/\/doi.org\/10.1111\/j.1469-8137.2011.04024.x","journal-title":"New Phytol"},{"key":"12613_CR100","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1007\/s12033-017-0022-y","volume":"59","author":"P Bharti","year":"2017","unstructured":"Bharti P, Jyoti P, Kapoor P, Sharma V, Shanmugam V, Yadav SK (2017) Host-Induced Silencing of Pathogenicity Genes Enhances Resistance to Fusarium oxysporum Wilt in Tomato. Mol Biotechnol 59:343\u2013352. https:\/\/doi.org\/10.1007\/s12033-017-0022-y","journal-title":"Mol Biotechnol"},{"key":"12613_CR101","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1111\/pbi.12307","volume":"13","author":"M Govindarajulu","year":"2015","unstructured":"Govindarajulu M, Epstein L, Wroblewski T, Michelmore RW (2015) Host-induced gene silencing inhibits the biotrophic pathogen causing downy mildew of lettuce. Plant Biotechnol J 13:875\u2013883. https:\/\/doi.org\/10.1111\/pbi.12307","journal-title":"Plant Biotechnol J"},{"key":"12613_CR102","doi-asserted-by":"crossref","unstructured":"Safarova D, Brazda P, Navratil M (2014) Effect of Artificial dsRNA on Infection of Pea Plants by Pea seed-borne mosaic virus. J Genet Plant Breed 50:105\u2013108","DOI":"10.17221\/120\/2013-CJGPB"},{"key":"12613_CR103","doi-asserted-by":"publisher","first-page":"265","DOI":"10.3389\/fpls.2019.00265","volume":"10","author":"EA Worrall","year":"2019","unstructured":"Worrall EA, Bravo-Cazar A, Nilon AT, Fletcher SJ, Robinson KE, Carr JP, Mitter N (2019) Exogenous application of RNAi-inducing double-stranded RNA inhibits aphid-mediated transmission of a plant virus. Front Plant Sci 10:265. https:\/\/doi.org\/10.3389\/fpls.2019.00265","journal-title":"Front Plant Sci"},{"key":"12613_CR104","doi-asserted-by":"publisher","first-page":"101684","DOI":"10.1016\/j.pmpp.2021.101684","volume":"115","author":"MA Mosa","year":"2021","unstructured":"Mosa MA, Youssef K (2021) Topical delivery of host induced RNAi silencing by layered double hydroxide nanosheets: An efficient tool to decipher pathogenicity gene function of Fusarium crown and root rot in tomato. Physiol Mol Plant Pathol 115:101684. https:\/\/doi.org\/10.1016\/j.pmpp.2021.101684","journal-title":"Physiol Mol Plant Pathol"},{"key":"12613_CR105","doi-asserted-by":"publisher","first-page":"194","DOI":"10.3390\/agronomy15010194","volume":"15","author":"M Spada","year":"2025","unstructured":"Spada M, Pugliesi C, Fambrini M, Palpacelli D, Caneo A, Pecchia S (2025) Spray-Induced Gene Silencing (SIGS): Nanocarrier-Mediated dsRNA Delivery Improves RNAi Efficiency in the Management of Lettuce Gray Mold Caused by Botrytis cinerea. Agronomy 15:194. https:\/\/doi.org\/10.3390\/agronomy15010194","journal-title":"Agronomy"},{"key":"12613_CR106","doi-asserted-by":"publisher","first-page":"7915","DOI":"10.3390\/ijms23147915","volume":"23","author":"VO Samarskaya","year":"2022","unstructured":"Samarskaya VO, Spechenkova N, Markin N, Suprunova TP, Zavriev SK, Love AJ, Taliansky M (2022) Impact of exogenous application of potato virus Y-specific dsRNA on RNA interference, pattern-triggered immunity and poly (ADP-ribose) metabolism. Int J Mol Sci 23:7915. https:\/\/doi.org\/10.3390\/ijms23147915","journal-title":"Int J Mol Sci"},{"key":"12613_CR107","doi-asserted-by":"publisher","first-page":"2332","DOI":"10.3390\/agronomy15102332","volume":"15","author":"Y Wang","year":"2025","unstructured":"Wang Y, Liu L, Fan Y, Han Y, Liang Z, Geng Y, Luo C (2025) Exogenous Application of IR-Specific dsRNA Inhibits Infection of Cucumber Green Mottle Mosaic Virus in Watermelon. Agronomy 15:2332. https:\/\/doi.org\/10.3390\/agronomy15102332","journal-title":"Agronomy"},{"key":"12613_CR108","doi-asserted-by":"publisher","first-page":"114661","DOI":"10.1016\/j.scienta.2026.114661","volume":"357","author":"MJ Kim","year":"2026","unstructured":"Kim MJ, Lee HJ, Yoon JY, Hong JS, Jeong RD (2026) Green nanoparticles-assisted dsRNA delivery enables effective control of pepper mottle virus in pepper. Sci Hortic 357:114661. https:\/\/doi.org\/10.1016\/j.scienta.2026.114661","journal-title":"Sci Hortic"},{"key":"12613_CR109","doi-asserted-by":"crossref","unstructured":"Yin Y, Wang R, Hou J, Fu Y, Chen Y, Wang L, Liu T (2026) Nanomaterial-mediated delivery of tomato miR393 intrinsically targeting a cell wall-related gene of Botrytis cinerea confers efficient control of gray mold disease. Plant Commun 7:101615","DOI":"10.1016\/j.xplc.2025.101615"},{"key":"12613_CR110","doi-asserted-by":"publisher","first-page":"e04889","DOI":"10.1002\/advs.202504889","volume":"13","author":"X Liu","year":"2026","unstructured":"Liu X, Liang X, Cai Z, Liu Z, Wang X, Zhou C, Liu S (2026) BioCNTs mediated delivery of systemically mobile small RNAs via leaf spray to control both tomato DNA and RNA viruses. Adv Sci 13:e04889. https:\/\/doi.org\/10.1002\/advs.202504889","journal-title":"Adv Sci"},{"key":"12613_CR111","doi-asserted-by":"publisher","first-page":"114456","DOI":"10.1016\/j.jviromet.2022.114456","volume":"301","author":"N Yamagishi","year":"2022","unstructured":"Yamagishi N, Yoshikawa N (2022) Efficient virus-induced gene silencing system in pumpkin (Cucurbita maxima) using apple latent spherical virus vector. J Virol Methods 301:114456. https:\/\/doi.org\/10.1016\/j.jviromet.2022.114456","journal-title":"J Virol Methods"},{"key":"12613_CR112","doi-asserted-by":"publisher","first-page":"490","DOI":"10.1094\/PHYTO.2004.94.5.490","volume":"94","author":"Y Yang","year":"2004","unstructured":"Yang Y, Sherwood TA, Patte CP, Hiebert E, Polston JE (2004) Use of Tomato yellow leaf curl virus (TYLCV) Rep gene sequences to engineer TYLCV resistance in tomato. Phytopathology 94:490\u2013496. https:\/\/doi.org\/10.1094\/PHYTO.2004.94.5.490","journal-title":"Phytopathology"},{"key":"12613_CR113","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1186\/s13007-021-00775-w","volume":"17","author":"D Tuo","year":"2021","unstructured":"Tuo D, Zhou P, Yan P, Cui H, Liu Y, Wang H, Shen W (2021) A cassava common mosaic virus vector for virus-induced gene silencing in cassava. Plant Methods 17:74. https:\/\/doi.org\/10.1186\/s13007-021-00775-w","journal-title":"Plant Methods"},{"key":"12613_CR114","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1007\/s44307-024-00031-x","volume":"2","author":"JH Zhao","year":"2024","unstructured":"Zhao JH, Liu QY, Xie ZM, Guo HS (2024) Exploring the challenges of RNAi-based strategies for crop protection. Adv Biotechnol 2:23. https:\/\/doi.org\/10.1007\/s44307-024-00031-x","journal-title":"Adv Biotechnol"},{"key":"12613_CR115","first-page":"536","volume":"102495","author":"C GRAY","year":"2025","unstructured":"GRAY C, Messier E, Chitwood-Brown J (2025) 109th Annual Meeting of The Potato Association of America, Abstracts and Posters, Madison, Wisconsin, USA,July27\u201331,2025. Am J Potato Res 102495:536","journal-title":"Am J Potato Res"},{"key":"12613_CR116","doi-asserted-by":"publisher","first-page":"e9321","DOI":"10.2903\/j.efsa.2025.9321","volume":"23","author":"EFSA GMO Panel","year":"2025","unstructured":"EFSA GMO Panel (2025) Risk assessment considerations for RNAi-based genetically modified plants. EFSA J 23:e9321. https:\/\/doi.org\/10.2903\/j.efsa.2025.9321","journal-title":"EFSA J"},{"key":"12613_CR117","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1038\/s44264-025-00057-1","volume":"3","author":"R Tardin-Coelho","year":"2025","unstructured":"Tardin-Coelho R, Fletcher S, Manzie N, Gunasekara SN, Fidelman P, Mitter N, Ashworth P (2025) A systematic review on public perceptions of RNAi-based biopesticides: Developing social licence to operate. Npj Sustain Agric 3:15. https:\/\/doi.org\/10.1038\/s44264-025-00057-1","journal-title":"Npj Sustain Agric"},{"key":"12613_CR118","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1007\/s42764-024-00135-7","volume":"5","author":"S Malakondaiah","year":"2024","unstructured":"Malakondaiah S, Julius A, Ponnambalam D, Gunthoti SS, Ashok J, Krishana PS, Rebecca J (2024) Gene silencing by RNA interference: A review. Genome Instab Dis 5:225\u2013241. https:\/\/doi.org\/10.1007\/s42764-024-00135-7","journal-title":"Genome Instab Dis"},{"key":"12613_CR119","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1126\/science.1231143","volume":"339","author":"L Cong","year":"2013","unstructured":"Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Zhang F (2013) Multiplex genome engineering using CRISPR\/Cas systems. Science 339:819\u2013823. https:\/\/doi.org\/10.1126\/science.1231143","journal-title":"Science"},{"key":"12613_CR120","doi-asserted-by":"publisher","first-page":"12288","DOI":"10.1128\/JVI.75.24.12288-12297.2001","volume":"75","author":"F Tenllado","year":"2001","unstructured":"Tenllado F, D\u00edaz-Ru\u00edz JR (2001) Double-stranded RNA-mediated interference with plant virus infection. Virol J 75:12288\u201312297. https:\/\/doi.org\/10.1128\/JVI.75.24.12288-12297.2001","journal-title":"Virol J"},{"key":"12613_CR121","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1111\/pbi.12226","volume":"12","author":"A Koch","year":"2014","unstructured":"Koch A, Kogel KH (2014) New wind in the sails: Improving the agronomic value of crop plants through RNAi-mediated gene silencing. Plant Biotechnol J 12:821\u2013831. https:\/\/doi.org\/10.1111\/pbi.12226","journal-title":"Plant Biotechnol J"},{"key":"12613_CR122","doi-asserted-by":"publisher","first-page":"1262","DOI":"10.1016\/j.cell.2014.05.010","volume":"157","author":"PD Hsu","year":"2014","unstructured":"Hsu PD, Lander ES, Zhang F (2014) Development and applications of CRISPR-Cas9 for genome engineering. Cell 157:1262\u20131278. https:\/\/doi.org\/10.1016\/j.cell.2014.05.010","journal-title":"Cell"},{"key":"12613_CR123","doi-asserted-by":"publisher","first-page":"1140","DOI":"10.1111\/mpp.12375","volume":"17","author":"J Chandrasekaran","year":"2016","unstructured":"Chandrasekaran J, Brumin M, Wolf D, Leibman D, Klap C, Pearlsman M, Sherman A, Arazi T, Gal-On A (2016) Development of broad virus resistance in non\u2010transgenic cucumber using CRISPR\/Cas9 technology. Mol Plant Pat 17:1140\u20131153","journal-title":"Mol Plant Pat"},{"key":"12613_CR124","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1038\/s41598-017-00578-x","volume":"7","author":"V Nekrasov","year":"2017","unstructured":"Nekrasov V, Wang C, Win J, Lanz C, Weigel D, Kamoun S (2017) Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion. Sci Rep 7:482. https:\/\/doi.org\/10.1038\/s41598-017-00578-x","journal-title":"Sci Rep"},{"key":"12613_CR125","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1016\/j.cell.2013.02.022","volume":"152","author":"LS Qi","year":"2013","unstructured":"Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell 152:1173\u20131183. https:\/\/doi.org\/10.1016\/j.cell.2013.02.022","journal-title":"Cell"},{"key":"12613_CR126","doi-asserted-by":"publisher","first-page":"291","DOI":"10.3390\/ijms17030291","volume":"17","author":"U Unniyampurath","year":"2016","unstructured":"Unniyampurath U, Pilankatta R, Krishnan MN (2016) RNA interference in the age of CRISPR: Will CRISPR interfere with RNAi? Int J Mol Sci 17:291. https:\/\/doi.org\/10.3390\/ijms17030291","journal-title":"Int J Mol Sci"},{"key":"12613_CR127","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.biotechadv.2014.12.006","volume":"33","author":"L Bortesi","year":"2015","unstructured":"Bortesi L, Fischer R (2015) The CRISPR\/Cas9 system for plant genome editing and beyond. Biotechnol Adv 33:41\u201352. https:\/\/doi.org\/10.1016\/j.biotechadv.2014.12.006","journal-title":"Biotechnol Adv"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-026-12613-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-026-12613-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-026-12613-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:41:37Z","timestamp":1787740897000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-026-12613-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,21]]},"references-count":127,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["12613"],"URL":"https:\/\/doi.org\/10.1007\/s11033-026-12613-0","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,21]]},"assertion":[{"value":"29 July 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 August 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 August 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 August 2026","order":5,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Update","order":6,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Duplicate affiliation 3 has been removed","order":7,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable to the paper.","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"1434"}}