{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T11:17:03Z","timestamp":1788002223872,"version":"build-2784847793"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Medical and Health Technology Plan of Zhejiang Province","award":["2022KY978"],"award-info":[{"award-number":["2022KY978"]}]},{"name":"Hangzhou Joint Fund of Zhejiang Provincial Natural Science Foundation of China","award":["HZQN26H160003"],"award-info":[{"award-number":["HZQN26H160003"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LTGY24H160025"],"award-info":[{"award-number":["LTGY24H160025"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LQ20H310004"],"award-info":[{"award-number":["LQ20H310004"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82103787"],"award-info":[{"award-number":["82103787"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82003017"],"award-info":[{"award-number":["82003017"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Construction Fund of Key Medical Disciplines of Hangzhou","award":["2025HZGF06"],"award-info":[{"award-number":["2025HZGF06"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1007\/s11033-026-12679-w","type":"journal-article","created":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T10:37:45Z","timestamp":1787999865000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cyclofenil blocks the secretion of IL-1\u03b2 by inhibiting assembly of the NLRP3 inflammasome independent of estrogen receptor"],"prefix":"10.1007","volume":"53","author":[{"given":"Jieqiong","family":"Feng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youyou","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiafeng","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenglin","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1582-9175","authenticated-orcid":false,"given":"Shujun","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,29]]},"reference":[{"issue":"8","key":"12679_CR1","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1016\/j.tips.2022.04.003","volume":"43","author":"RC Coll","year":"2022","unstructured":"Coll RC, Schroder K, Pelegr\u00edn P (2022) NLRP3 and pyroptosis blockers for treating inflammatory diseases. Trends Pharmacol Sci 43(8):653\u2013668","journal-title":"Trends Pharmacol Sci"},{"issue":"8","key":"12679_CR2","doi-asserted-by":"publisher","first-page":"588","DOI":"10.1038\/nrd.2018.97","volume":"17","author":"MSJ Mangan","year":"2018","unstructured":"Mangan MSJ, Olhava EJ, Roush WR, Seidel HM, Glick GD, Latz E (2018) Targeting the NLRP3 inflammasome in inflammatory diseases. Nat Rev Drug Discov 17(8):588\u2013606","journal-title":"Nat Rev Drug Discov"},{"key":"12679_CR3","doi-asserted-by":"publisher","first-page":"101482","DOI":"10.1016\/j.redox.2020.101482","volume":"31","author":"DN Di Florio","year":"2020","unstructured":"Di Florio DN, Sin J, Coronado MJ, Atwal PS, Fairweather D (2020) Sex differences in inflammation, redox biology, mitochondria and autoimmunity. Redox Biol 31:101482","journal-title":"Redox Biol"},{"issue":"1","key":"12679_CR4","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1038\/s41392-022-00955-7","volume":"7","author":"P Kong","year":"2022","unstructured":"Kong P, Cui ZY, Huang XF, Zhang DD, Guo RJ, Han M (2022) Inflammation and atherosclerosis: signaling pathways and therapeutic intervention. Signal Transduct Target Ther 7(1):131","journal-title":"Signal Transduct Target Ther"},{"issue":"9","key":"12679_CR5","doi-asserted-by":"publisher","first-page":"6425","DOI":"10.1002\/jcp.26429","volume":"233","author":"A Shapouri-Moghaddam","year":"2018","unstructured":"Shapouri-Moghaddam A, Mohammadian S, Vazini H, Taghadosi M, Esmaeili SA, Mardani F, Seifi B, Mohammadi A, Afshari JT, Sahebkar A (2018) Macrophage plasticity, polarization, and function in health and disease. J Cell Physiol 233(9):6425\u20136440","journal-title":"J Cell Physiol"},{"key":"12679_CR6","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1146\/annurev.immunol.021908.132715","volume":"27","author":"F Martinon","year":"2009","unstructured":"Martinon F, Mayor A, Tschopp J (2009) The inflammasomes: guardians of the body. Annu Rev Immunol 27:229\u2013265","journal-title":"Annu Rev Immunol"},{"issue":"11","key":"12679_CR7","doi-asserted-by":"publisher","first-page":"1201","DOI":"10.1038\/s41423-022-00922-w","volume":"19","author":"A Akbal","year":"2022","unstructured":"Akbal A, Dernst A, Lovotti M, Mangan MSJ, McManus RM, Latz E (2022) How location and cellular signaling combine to activate the NLRP3 inflammasome. Cell Mol Immunol 19(11):1201\u20131214","journal-title":"Cell Mol Immunol"},{"key":"12679_CR8","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1146\/annurev-immunol-081022-021207","volume":"41","author":"J Fu","year":"2023","unstructured":"Fu J, Wu H (2023) Structural Mechanisms of NLRP3 Inflammasome Assembly and Activation. Annu Rev Immunol 41:301\u2013316","journal-title":"Annu Rev Immunol"},{"issue":"5","key":"12679_CR9","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1038\/s41423-021-00670-3","volume":"18","author":"S Paik","year":"2021","unstructured":"Paik S, Kim JK, Silwal P, Sasakawa C, Jo EK (2021) An update on the regulatory mechanisms of NLRP3 inflammasome activation. Cell Mol Immunol 18(5):1141\u20131160","journal-title":"Cell Mol Immunol"},{"issue":"7285","key":"12679_CR10","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1038\/nature08780","volume":"464","author":"Q Zhang","year":"2010","unstructured":"Zhang Q, Raoof M, Chen Y, Sumi Y, Sursal T, Junger W, Brohi K, Itagaki K, Hauser CJ (2010) Circulating mitochondrial DAMPs cause inflammatory responses to injury. Nature 464(7285):104\u2013107","journal-title":"Nature"},{"issue":"7329","key":"12679_CR11","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1038\/nature09663","volume":"469","author":"R Zhou","year":"2011","unstructured":"Zhou R, Yazdi AS, Menu P, Tschopp J (2011) A role for mitochondria in NLRP3 inflammasome activation. Nature 469(7329):221\u2013225","journal-title":"Nature"},{"issue":"3","key":"12679_CR12","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1016\/j.cell.2016.05.076","volume":"166","author":"AJ Wolf","year":"2016","unstructured":"Wolf AJ, Reyes CN, Liang W, Becker C, Shimada K, Wheeler ML, Cho HC, Popescu NI, Coggeshall KM, Arditi M, Underhill DM (2016) Hexokinase Is an Innate Immune Receptor for the Detection of Bacterial Peptidoglycan. Cell 166(3):624\u2013636","journal-title":"Cell"},{"issue":"6571","key":"12679_CR13","doi-asserted-by":"publisher","first-page":"1076","DOI":"10.1126\/science.abi5934","volume":"374","author":"K Newton","year":"2021","unstructured":"Newton K, Dixit VM, Kayagaki N (2021) Dying cells fan the flames of inflammation. Science 374(6571):1076\u20131080","journal-title":"Science"},{"issue":"4","key":"12679_CR14","doi-asserted-by":"publisher","first-page":"268","DOI":"10.3109\/13697139909038087","volume":"2","author":"SL Silfen","year":"1999","unstructured":"Silfen SL, Ciaccia AV, Bryant HU (1999) Selective estrogen receptor modulators: tissue selectivity and differential uterine effects. Climacteric 2(4):268\u2013283","journal-title":"Climacteric"},{"issue":"1","key":"12679_CR15","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1007\/s00705-018-4079-0","volume":"164","author":"D Tohma","year":"2019","unstructured":"Tohma D, Tajima S, Kato F, Sato H, Kakisaka M, Hishiki T, Kataoka M, Takeyama H, Lim CK, Aida Y, Saijo M (2019) An estrogen antagonist, cyclofenil, has anti-dengue-virus activity. Arch Virol 164(1):225\u2013234","journal-title":"Arch Virol"},{"issue":"2","key":"12679_CR16","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1006\/gyno.1996.0030","volume":"60","author":"S Batra","year":"1996","unstructured":"Batra S, Iosif CS (1996) Elevated concentrations of antiestrogen binding sites in membrane fractions of human ovarian tumors. Gynecol Oncol 60(2):228\u2013232","journal-title":"Gynecol Oncol"},{"issue":"8","key":"12679_CR17","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.1021\/jm00158a006","volume":"29","author":"MR Schneider","year":"1986","unstructured":"Schneider MR, Ball H, Schiller CD (1986) Catechol estrogens of the 1,1,2-triphenylbut-1-ene type: relationship between structure, estradiol receptor affinity, estrogenic and antiestrogenic properties, and mammary tumor inhibiting activities. J Med Chem 29(8):1355\u20131362","journal-title":"J Med Chem"},{"issue":"10","key":"12679_CR18","first-page":"4392","volume":"44","author":"A Ramu","year":"1984","unstructured":"Ramu A, Glaubiger D, Fuks Z (1984) Reversal of acquired resistance to doxorubicin in P388 murine leukemia cells by tamoxifen and other triparanol analogues. Cancer Res 44(10):4392\u20134395","journal-title":"Cancer Res"},{"issue":"3","key":"12679_CR19","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.cbi.2003.06.001","volume":"146","author":"HJ Martin","year":"2003","unstructured":"Martin HJ, Kornmann F, Fuhrmann GF (2003) The inhibitory effects of flavonoids and antiestrogens on the Glut1 glucose transporter in human erythrocytes. Chem Biol Interact 146(3):225\u2013235","journal-title":"Chem Biol Interact"},{"key":"12679_CR20","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.molimm.2022.11.009","volume":"153","author":"S Xie","year":"2023","unstructured":"Xie S, Liang J, Zhao Y, Zhang J, Chen X, Jiang H, Zhang Z, Ma S, Zhang S (2023) The second-generation tyrosine kinase inhibitor afatinib inhibits IL-1\u03b2 secretion via blocking assembly of NLRP3 inflammasome independent of epidermal growth factor receptor signaling in macrophage. Mol Immunol 153:135\u2013145","journal-title":"Mol Immunol"},{"issue":"8","key":"12679_CR21","doi-asserted-by":"publisher","first-page":"2496","DOI":"10.1021\/jm0512037","volume":"49","author":"JW Seo","year":"2006","unstructured":"Seo JW, Comninos JS, Chi DY, Kim DW, Carlson KE, Katzenellenbogen JA (2006) Fluorine-substituted cyclofenil derivatives as estrogen receptor ligands: synthesis and structure-affinity relationship study of potential positron emission tomography agents for imaging estrogen receptors in breast cancer. J Med Chem 49(8):2496\u20132511","journal-title":"J Med Chem"},{"key":"12679_CR22","doi-asserted-by":"publisher","first-page":"106068","DOI":"10.1016\/j.biocel.2021.106068","volume":"139","author":"Q Jiang","year":"2021","unstructured":"Jiang Q, Li W, Zhu X, Yu L, Lu Z, Liu Y, Ma B, Cheng L (2021) Estrogen receptor \u03b2 alleviates inflammatory lesions in a rat model of inflammatory bowel disease via down-regulating P2X7R expression in macrophages. Int J Biochem Cell Biol 139:106068","journal-title":"Int J Biochem Cell Biol"},{"key":"12679_CR23","doi-asserted-by":"publisher","first-page":"701391","DOI":"10.3389\/fcimb.2021.701391","volume":"11","author":"C Souza","year":"2021","unstructured":"Souza C, Barbosa CD, Coelho H, Santos J\u00fanior MN, Barbosa EN, Queiroz C, Teles \u00c9MF, Dos Santos DC, Bittencourt RS, Soares TJ, Oliveira MV, Timenetsky J, Campos GB, Marques LM (2021) Effects of 17\u03b2-Estradiol on Monocyte\/Macrophage Response to Staphylococcus aureus: An In Vitro Study. Frontiers cell infect microbiol 11:701391","journal-title":"Frontiers cell infect microbiol"},{"key":"12679_CR24","doi-asserted-by":"publisher","first-page":"115566","DOI":"10.1016\/j.intimp.2025.115566","volume":"166","author":"R Li","year":"2025","unstructured":"Li R, Fan X, He Z, Shen H, Wu H, Chen J, Peng L, Liang B, Feng C, Zhu H (2025) IL-1\u03b2-stimulated bone mesenchymal stem cell-derived exosomes promote cutaneous wound healing by inhibiting SIRT6\/NLRP3 pathway. Int Immunopharmacol 166:115566","journal-title":"Int Immunopharmacol"},{"issue":"1","key":"12679_CR25","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1186\/s13075-025-03679-5","volume":"27","author":"D Cai","year":"2025","unstructured":"Cai D, Zhong C, Yang Z, Li J, Hong S (2025) Bone marrow mesenchymal stem cell-derived exosomes improve pyroptosis and mitochondrial integrity through miR-515-5p-mediated TLR4\/NLRP3\/GSDMD axis in rheumatoid arthritis. Arthritis Res Therapy 27(1):226","journal-title":"Arthritis Res Therapy"},{"key":"12679_CR26","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-026-05152-9","author":"Y Zhang","year":"2026","unstructured":"Zhang Y, Wang F, Yu H, Jiang C, Li Z, Bu R, Yu J (2026) Extracellular vesicles derived from engineered BMSCs improve damaged cartilage in mice with osteoarthritis by delivering PBX1. LID. Stem Cell Res Ther. https:\/\/doi.org\/10.1186\/s13287-026-05152-9","journal-title":"Stem Cell Res Ther"},{"key":"12679_CR27","doi-asserted-by":"publisher","first-page":"103176","DOI":"10.1016\/j.mtbio.2026.103176","volume":"9","author":"Y Ma","year":"2026","unstructured":"Ma Y, He S, Liu H, Zhang J, Huang W, Gu Y, Cui N, Qian J, Lu M, Xu D, Li Z, Yu B (2026) A honeysuckle-decorated nanoimmunomodulator hijacks Macrophage-BMSC crosstalk to promote arthritic regeneration. Mater Today Bio 9:103176","journal-title":"Mater Today Bio"},{"key":"12679_CR28","doi-asserted-by":"publisher","first-page":"156225","DOI":"10.1016\/j.phymed.2024.156225","volume":"135","author":"M Chen","year":"2024","unstructured":"Chen M, Wang R, Liao L, Li Y, Sun X, Wu H, Lan Q, Deng Z, Liu P, Xu T, Zhou H, Liu M (2024) DanShen Decoction targets miR-93-5p to provide protection against MI\/RI by regulating the TXNIP\/NLRP3\/Caspase-1 signaling pathway. Phytomedicine 135:156225","journal-title":"Phytomedicine"},{"issue":"28","key":"12679_CR29","doi-asserted-by":"publisher","first-page":"2301366","DOI":"10.1002\/adhm.202301366","volume":"12","author":"JA-O Luo","year":"2023","unstructured":"Luo JA-O, Chen H, Wang G, Lyu J, Liu Y, Lin S, Zhou M, Jiang X (2023) CGRP-Loaded Porous Microspheres Protect BMSCs for Alveolar Bone Regeneration in the Periodontitis Microenvironment. Adv Healthc Mater 12(28):2301366","journal-title":"Adv Healthc Mater"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-026-12679-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-026-12679-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-026-12679-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T10:37:46Z","timestamp":1787999866000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-026-12679-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,29]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["12679"],"URL":"https:\/\/doi.org\/10.1007\/s11033-026-12679-w","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,29]]},"assertion":[{"value":"18 May 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 August 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 August 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of the Zhejiang Center of Laboratory Animals (ZJCLA-IACUC-20010671).","order":2,"name":"Ethics","label":"Ethics approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously. Shirong Zhang, Shenglin Ma and Shujun Xie should be considered co-correspondence authors. All the authors listed have approved the manuscript that is enclosed. This manuscript is approved by all authors for publication. This work was approved by the local ethics committees and written informed consent was obtained from each subject. Neither the entire paper nor any part of its content has been published or has been accepted elsewhere. It is not being submitted to any other journal.","order":3,"name":"Ethics","label":"Consent for publication","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"1494"}}