{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,20]],"date-time":"2025-07-20T04:24:34Z","timestamp":1752985474192,"version":"3.37.3"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T00:00:00Z","timestamp":1705449600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T00:00:00Z","timestamp":1705449600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100017590","name":"Universit\u00e4tsklinikum Essen","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100017590","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mycopathologia"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The opportunistic black yeast-like fungus <jats:italic>Exophiala dermatitidis<\/jats:italic> frequently colonizes the respiratory tract of cystic fibroses (CF) patients. Additionally, it can cause superficial, systemic, and cerebral forms of phaeohyphomycoses. The objective of this study was to develop and apply a microsatellite or short tandem repeat (STR) genotyping scheme for <jats:italic>E. dermatitidis<\/jats:italic>. In total, 82 <jats:italic>E. dermatitidis<\/jats:italic> isolates from various geographic origins (environmental\u2009=\u20099, CF\u2009=\u200963, invasive isolates\u2009=\u20099, melanin-deficient mutant\u2009=\u20091) were included in this study. After next-generation sequencing of a reference strain and sequence filtering for microsatellites, six STR markers were selected and amplified in two multiplex PCR reactions. The included isolates were discriminated in a genetic cluster analysis using the Pearson algorithm\u00a0to reveal the relatedness of the isolates. The <jats:italic>E. dermatitidis<\/jats:italic> isolates clustered on basis of both, their source and their origin. The invasive isolates from Asia were unrelated to isolates from CF. Nearly all environmental isolates were grouped separately from patients\u2019 isolates. The Simpson index was 0.94. In conclusion, we were able to establish a STR genotyping scheme for investigating population genomics of <jats:italic>E. dermatitidis.<\/jats:italic><\/jats:p>","DOI":"10.1007\/s11046-023-00825-y","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T13:02:00Z","timestamp":1705496520000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Establishment of a Novel Short Tandem Repeat Typing Method for Exophiala dermatitidis"],"prefix":"10.1007","volume":"189","author":[{"given":"Hamide","family":"Zoqi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludwig","family":"Sedlacek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter-Michael","family":"Rath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joerg","family":"Steinmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2015-275X","authenticated-orcid":false,"given":"Lisa","family":"Kirchhoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,1,17]]},"reference":[{"issue":"5","key":"825_CR1","doi-asserted-by":"publisher","first-page":"854","DOI":"10.1093\/clinids\/5.5.854","volume":"5","author":"PE Hohl","year":"1983","unstructured":"Hohl PE, Holley HP, Prevost E, Ajello L, Padhye AA. Infections due to Wangiella dermatitidis in humans: report of the first documented case from the United States and a review of the literature. Rev Infect Dis. 1983;5(5):854\u201364.","journal-title":"Rev Infect Dis"},{"key":"825_CR2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2019.1596504","author":"L Kirchhoff","year":"2019","unstructured":"Kirchhoff L, Olsowski M, Rath PM, Steinmann J. Exophiala dermatitidis: key issues of an opportunistic fungal pathogen. Virulence. 2019. https:\/\/doi.org\/10.1080\/21505594.2019.1596504.","journal-title":"Virulence"},{"key":"825_CR3","doi-asserted-by":"publisher","DOI":"10.1038\/srep42886","author":"L Kirchhoff","year":"2017","unstructured":"Kirchhoff L, Olsowski M, Zilmans K, Dittmer S, Haase G, Sedlacek L, et al. Biofilm formation of the black yeast-like fungus Exophiala dermatitidis and its susceptibility to antiinfective agents. Sci Rep. 2017. https:\/\/doi.org\/10.1038\/srep42886.","journal-title":"Sci Rep"},{"issue":"2","key":"825_CR4","doi-asserted-by":"publisher","first-page":"e0148166","DOI":"10.1371\/journal.pone.0148166","volume":"11","author":"J Zupan\u010di\u010d","year":"2016","unstructured":"Zupan\u010di\u010d J, Novak Babi\u010d M, Zalar P, Gunde-Cimerman N. The black yeast Exophiala dermatitidis and other selected opportunistic human fungal pathogens spread from dishwashers to kitchens. PLoS ONE. 2016;11(2):e0148166. https:\/\/doi.org\/10.1371\/journal.pone.0148166.","journal-title":"PLoS ONE"},{"key":"825_CR5","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02514","author":"Y Song","year":"2017","unstructured":"Song Y, Laureijssen-van de Sande WWJ, Moreno LF, Gerrits van den Ende B, Li R, de Hoog S. Comparative ecology of capsular Exophiala species causing disseminated infection in humans. Front Microbiol. 2017. https:\/\/doi.org\/10.3389\/fmicb.2017.02514.","journal-title":"Front Microbiol"},{"issue":"11","key":"825_CR6","doi-asserted-by":"publisher","first-page":"3713","DOI":"10.1128\/JCM.02012-06","volume":"45","author":"JS Zeng","year":"2007","unstructured":"Zeng JS, Sutton DA, Fothergill AW, Rinaldi MG, Harrak MJ, de Hoog GS. Spectrum of Clinically relevant Exophiala species in the United States. J Clin Microbiol. 2007;45(11):3713\u201320. https:\/\/doi.org\/10.1128\/JCM.02012-06.","journal-title":"J Clin Microbiol"},{"issue":"4","key":"825_CR7","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1093\/mmy\/myu093","volume":"53","author":"B \u00d6zhak-Baysan","year":"2015","unstructured":"\u00d6zhak-Baysan B, \u00d6\u011f\u00fcn\u00e7 D, D\u00f6\u011fen A, Ilkit M, de Hoog GS. MALDI-TOF MS-based identification of black yeasts of the genus Exophiala. Med Mycol. 2015;53(4):347\u201352. https:\/\/doi.org\/10.1093\/mmy\/myu093.","journal-title":"Med Mycol"},{"issue":"1","key":"825_CR8","doi-asserted-by":"publisher","first-page":"myv085-myv","DOI":"10.1093\/mmy\/myv085","volume":"54","author":"M Fraser","year":"2015","unstructured":"Fraser M, Brown Z, Houldsworth M, Borman AM, Johnson EM. Rapid identification of 6328 isolates of pathogenic yeasts using MALDI-ToF MS and a simplified, rapid extraction procedure that is compatible with the Bruker Biotyper platform and database. Med Mycol. 2015;54(1):myv085-myv. https:\/\/doi.org\/10.1093\/mmy\/myv085.","journal-title":"Med Mycol"},{"issue":"10","key":"825_CR9","doi-asserted-by":"publisher","first-page":"e25712-e","DOI":"10.1371\/journal.pone.0025712","volume":"6","author":"A Pinto","year":"2011","unstructured":"Pinto A, Halliday C, Zahra M, van Hal S, Olma T, Maszewska K, et al. Matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of yeasts is contingent on robust Rreference spectra. PLoS ONE. 2011;6(10):e25712-e. https:\/\/doi.org\/10.1371\/journal.pone.0025712.","journal-title":"PLoS ONE"},{"issue":"6","key":"825_CR10","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1016\/j.jcf.2008.05.004","volume":"7","author":"Y Nagano","year":"2008","unstructured":"Nagano Y, Elborn JS, Millar BC, Goldsmith CE, Rendall J, Moore JE. Development of a novel PCR assay for the identification of the black yeast, Exophiala (Wangiella) dermatitidis from adult patients with cystic fibrosis (CF). J Cyst Fibros. 2008;7(6):576\u201380. https:\/\/doi.org\/10.1016\/j.jcf.2008.05.004.","journal-title":"J Cyst Fibros"},{"issue":"2","key":"825_CR11","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1007\/s00248-015-0670-z","volume":"71","author":"\u00c7 Ergin","year":"2016","unstructured":"Ergin \u00c7, G\u00f6k Y, Bay\u011fu Y, G\u00fcmral R, \u00d6zhak-Baysan B, D\u00f6\u011fen A, et al. ATR-FTIR spectroscopy highlights the problem of distinguishing between Exophiala dermatitidis and E. phaeomuriformis using MALDI-TOF MS. Microb Ecol. 2016;71(2):339\u201346. https:\/\/doi.org\/10.1007\/s00248-015-0670-z.","journal-title":"Microb Ecol"},{"issue":"5","key":"825_CR12","doi-asserted-by":"publisher","first-page":"475","DOI":"10.3109\/13693786.2010.539630","volume":"49","author":"J Steinmann","year":"2011","unstructured":"Steinmann J, Schmidt D, Buer J, Rath PM. Discrimination of Scedosporium prolificans against Pseudallescheria boydii and Scedosporium apiospermum by semiautomated repetitive sequence-based PCR. Med Mycol. 2011;49(5):475\u201383. https:\/\/doi.org\/10.3109\/13693786.2010.539630.","journal-title":"Med Mycol"},{"issue":"9","key":"825_CR13","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1099\/00222615-46-9-757","volume":"46","author":"PM Rath","year":"1997","unstructured":"Rath PM, M\u00fcller KD, Dermoumi H, Ansorg R. A comparison of methods of phenotypic and genotypic fingerprinting of Exophiala dermatitidis isolated from sputum samples of patients with cystic fibrosis. J Med Microbiol. 1997;46(9):757\u201362. https:\/\/doi.org\/10.1099\/00222615-46-9-757.","journal-title":"J Med Microbiol"},{"issue":"9","key":"825_CR14","doi-asserted-by":"publisher","first-page":"1226","DOI":"10.1099\/jmm.0.042317-0","volume":"61","author":"A Packeu","year":"2012","unstructured":"Packeu A, Lebecque P, Rodriguez-Villalobos H, Boeras A, Hendrickx M, Bouchara J-P, Symoens F. Molecular typing and antifungal susceptibility of Exophiala isolates from patients with cystic fibrosis. J Med Microbiol. 2012;61(9):1226\u201333. https:\/\/doi.org\/10.1099\/jmm.0.042317-0.","journal-title":"J Med Microbiol"},{"key":"825_CR15","unstructured":"Centers for Disease C, Prevention. Exophiala infection from contaminated injectable steroids prepared by a compounding pharmacy-United States, July-November 2002. MMWR Morb Mortal wkl Rep. 2002;51(49):1109\u201312."},{"key":"825_CR16","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix948","author":"A Vasquez","year":"2017","unstructured":"Vasquez A, Zavasky D, Chow NA, Gade L, Zlatanic E, Elkind S, et al. Management of an outbreak of Exophiala dermatitidis bloodstream infections at an outpatient oncology clinic. Clin Infect Dis. 2017. https:\/\/doi.org\/10.1093\/cid\/cix948.","journal-title":"Clin Infect Dis"}],"container-title":["Mycopathologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11046-023-00825-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11046-023-00825-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11046-023-00825-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T17:21:11Z","timestamp":1709227271000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11046-023-00825-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,17]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["825"],"URL":"https:\/\/doi.org\/10.1007\/s11046-023-00825-y","relation":{},"ISSN":["0301-486X","1573-0832"],"issn-type":[{"type":"print","value":"0301-486X"},{"type":"electronic","value":"1573-0832"}],"subject":[],"published":{"date-parts":[[2024,1,17]]},"assertion":[{"value":"3 October 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 December 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 January 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"5"}}