{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T12:40:10Z","timestamp":1758976810672,"version":"3.44.0"},"reference-count":62,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Jingzhou Science and Technology Plan Projec","award":["2024HD34]"],"award-info":[{"award-number":["2024HD34]"]}]},{"name":"Yangtze University Science and Technology Aid to Tibet Medical Talent Training Program Projec","award":["2023YZ06"],"award-info":[{"award-number":["2023YZ06"]}]},{"name":"Key Research and Development program of Hubei Provinc","award":["2024BCB043"],"award-info":[{"award-number":["2024BCB043"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mycopathologia"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1007\/s11046-025-00993-z","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T17:28:38Z","timestamp":1757611718000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Isolation of a Multi-azole-Resistant Aspergillus fumigatus cyp51A TR46\/Y46F\/F70L Mutant in a Patient with Fungal Keratitis"],"prefix":"10.1007","volume":"190","author":[{"given":"Xiang","family":"Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqi","family":"Mao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhao","family":"Ling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Ge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Menghua","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiqin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,9,11]]},"reference":[{"key":"993_CR1","doi-asserted-by":"publisher","DOI":"10.1101\/2021.04.19.440430","author":"K Ahmadikia","year":"2021","unstructured":"Ahmadikia K, Gharehbolagh SA, Fallah B, Naeimi M, Malekifar P, Rahsepar S, et al. Distribution, prevalence, and causative agents of fungal keratitis: a systematic review and meta-analysis (1990 to 2020). Microbiology. 2021. https:\/\/doi.org\/10.1101\/2021.04.19.440430.","journal-title":"Microbiology"},{"issue":"3","key":"993_CR2","doi-asserted-by":"publisher","first-page":"e49","DOI":"10.1016\/S1473-3099(20)30448-5","volume":"21","author":"L Brown","year":"2021","unstructured":"Brown L, Leck AK, Gichangi M, Burton MJ, Denning DW. The global incidence and diagnosis of fungal keratitis. Lancet Infect Dis. 2021;21(3):e49\u201357. https:\/\/doi.org\/10.1016\/S1473-3099(20)30448-5.","journal-title":"Lancet Infect Dis"},{"key":"993_CR3","doi-asserted-by":"publisher","first-page":"1253197","DOI":"10.3389\/fmicb.2023.1253197","volume":"14","author":"M Zeng","year":"2023","unstructured":"Zeng M, Zhou X, Yang C, Liu Y, Zhang J, Xin C, et al. Comparative analysis of the biological characteristics and mechanisms of azole resistance of clinical Aspergillus fumigatus strains. Front Microbiol. 2023;14:1253197. https:\/\/doi.org\/10.3389\/fmicb.2023.1253197.","journal-title":"Front Microbiol"},{"key":"993_CR4","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1186\/1471-2180-8-9","volume":"8","author":"S Costa-de-Oliveira","year":"2008","unstructured":"Costa-de-Oliveira S, Araujo R, Silva-Dias A, Pina-Vaz C, Rodrigues AG. Propofol lipidic infusion promotes resistance to antifungals by reducing drug input into the fungal cell. BMC Microbiol. 2008;8:9. https:\/\/doi.org\/10.1186\/1471-2180-8-9.","journal-title":"BMC Microbiol"},{"issue":"6","key":"993_CR5","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1016\/j.ophtha.2007.07.034","volume":"115","author":"L Xie","year":"2008","unstructured":"Xie L, Zhai H, Shi W, Zhao J, Sun S, Zang X. Hyphal growth patterns and recurrence of fungal keratitis after lamellar keratoplasty. Ophthalmology. 2008;115(6):983\u20137. https:\/\/doi.org\/10.1016\/j.ophtha.2007.07.034.","journal-title":"Ophthalmology"},{"issue":"11","key":"993_CR6","doi-asserted-by":"publisher","first-page":"907","DOI":"10.3390\/jof7110907","volume":"7","author":"N Raj","year":"2021","unstructured":"Raj N, Vanathi M, Ahmed NH, Gupta N, Lomi N, Tandon R. Recent perspectives in the management of fungal keratitis. J Fungi. 2021;7(11):907. https:\/\/doi.org\/10.3390\/jof7110907.","journal-title":"J Fungi"},{"issue":"11","key":"993_CR7","doi-asserted-by":"publisher","first-page":"6896","DOI":"10.1128\/AAC.00683-16","volume":"60","author":"CF Neoh","year":"2016","unstructured":"Neoh CF, Leung L, Chan E, Al-Badriyeh D, Fullinfaw RO, Jhanji V, et al. Open-label study of absorption and clearance of 1% Voriconazole eye drops. Antimicrob Agents Chemother. 2016;60(11):6896\u20138. https:\/\/doi.org\/10.1128\/AAC.00683-16.","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"993_CR8","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1089\/jop.2018.0089","volume":"35","author":"P Lakhani","year":"2019","unstructured":"Lakhani P, Patil A, Majumdar S. Challenges in the polyene- and azole-based pharmacotherapy of ocular fungal infections. J Ocul Pharmacol Ther. 2019;35(1):6\u201322. https:\/\/doi.org\/10.1089\/jop.2018.0089.","journal-title":"J Ocul Pharmacol Ther"},{"key":"993_CR9","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.jtos.2021.12.001","volume":"24","author":"N Sharma","year":"2022","unstructured":"Sharma N, Bagga B, Singhal D, Nagpal R, Kate A, Saluja G, et al. Fungal keratitis: a review of clinical presentations, treatment strategies and outcomes. Ocul Surf. 2022;24:22\u201330. https:\/\/doi.org\/10.1016\/j.jtos.2021.12.001.","journal-title":"Ocul Surf"},{"issue":"10","key":"993_CR10","doi-asserted-by":"publisher","first-page":"1406","DOI":"10.1136\/bjo.2010.191734","volume":"95","author":"Y Matsumoto","year":"2011","unstructured":"Matsumoto Y, Murat D, Kojima T, Shimazaki J, Tsubota K. The comparison of solitary topical micafungin or fluconazole application in the treatment of Candida fungal keratitis. Br J Ophthalmol. 2011;95(10):1406\u20139. https:\/\/doi.org\/10.1136\/bjo.2010.191734.","journal-title":"Br J Ophthalmol"},{"issue":"16","key":"993_CR11","first-page":"1884","volume":"122","author":"QF Liang","year":"2009","unstructured":"Liang QF, Jin XY, Wang XL, Sun XG. Effect of topical application of terbinafine on fungal keratitis. Chin Med J. 2009;122(16):1884\u20138.","journal-title":"Chin Med J"},{"issue":"12","key":"993_CR12","doi-asserted-by":"publisher","first-page":"916","DOI":"10.1111\/myc.12822","volume":"61","author":"S Mahmoudi","year":"2018","unstructured":"Mahmoudi S, Masoomi A, Ahmadikia K, Tabatabaei SA, Soleimani M, Rezaie S, et al. Fungal keratitis: an overview of clinical and laboratory aspects. Mycoses. 2018;61(12):916\u201330. https:\/\/doi.org\/10.1111\/myc.12822.","journal-title":"Mycoses"},{"issue":"5","key":"993_CR13","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1089\/jop.2016.0186","volume":"33","author":"A Patil","year":"2017","unstructured":"Patil A, Majumdar S. Echinocandins in ocular therapeutics. J Ocul Pharmacol Ther. 2017;33(5):340\u201352. https:\/\/doi.org\/10.1089\/jop.2016.0186.","journal-title":"J Ocul Pharmacol Ther"},{"issue":"10","key":"993_CR14","doi-asserted-by":"publisher","first-page":"e1003633","DOI":"10.1371\/journal.ppat.1003633","volume":"9","author":"A Chowdhary","year":"2013","unstructured":"Chowdhary A, Kathuria S, Xu J, Meis JF. Emergence of azole-resistant aspergillus fumigatus strains due to agricultural azole use creates an increasing threat to human health. PLoS Pathog. 2013;9(10):e1003633. https:\/\/doi.org\/10.1371\/journal.ppat.1003633.","journal-title":"PLoS Pathog"},{"issue":"3","key":"993_CR15","doi-asserted-by":"publisher","first-page":"e00791","DOI":"10.1128\/mBio.00791-17","volume":"8","author":"J Zhang","year":"2017","unstructured":"Zhang J, Snelders E, Zwaan BJ, Schoustra SE, Meis JF, van Dijk K, et al. A novel environmental azole resistance mutation in Aspergillus fumigatus and a possible role of sexual reproduction in its emergence. MBio. 2017;8(3):e00791. https:\/\/doi.org\/10.1128\/mBio.00791-17.","journal-title":"MBio"},{"issue":"4","key":"993_CR16","doi-asserted-by":"publisher","first-page":"1299","DOI":"10.1111\/1462-2920.14055","volume":"20","author":"A Chowdhary","year":"2018","unstructured":"Chowdhary A, Meis JF. Emergence of azole resistant Aspergillus fumigatus and one health: time to implement environmental stewardship. Environ Microbiol. 2018;20(4):1299\u2013301. https:\/\/doi.org\/10.1111\/1462-2920.14055.","journal-title":"Environ Microbiol"},{"issue":"12","key":"993_CR17","doi-asserted-by":"publisher","first-page":"e383","DOI":"10.1016\/S1473-3099(17)30316-X","volume":"17","author":"DS Perlin","year":"2017","unstructured":"Perlin DS, Rautemaa-Richardson R, Alastruey-Izquierdo A. The global problem of antifungal resistance: prevalence, mechanisms, and management. Lancet Infect Dis. 2017;17(12):e383\u201392. https:\/\/doi.org\/10.1016\/S1473-3099(17)30316-X.","journal-title":"Lancet Infect Dis"},{"issue":"4","key":"993_CR18","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1007\/s12154-014-0121-1","volume":"7","author":"JE Parker","year":"2014","unstructured":"Parker JE, Warrilow AGS, Price CL, Mullins JGL, Kelly DE, Kelly SL. Resistance to antifungals that target CYP51. J Chem Biol. 2014;7(4):143\u201361. https:\/\/doi.org\/10.1007\/s12154-014-0121-1.","journal-title":"J Chem Biol"},{"issue":"6","key":"993_CR19","doi-asserted-by":"publisher","first-page":"e00241","DOI":"10.1128\/AAC.00241-18","volume":"62","author":"R Garcia-Rubio","year":"2018","unstructured":"Garcia-Rubio R, Alcazar-Fuoli L, Monteiro MC, Monzon S, Cuesta I, Pelaez T, et al. Insight into the significance of Aspergillus fumigatus cyp51A polymorphisms. Antimicrob Agents Chemother. 2018;62(6):e00241-e318. https:\/\/doi.org\/10.1128\/AAC.00241-18.","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"993_CR20","doi-asserted-by":"publisher","first-page":"e1012851","DOI":"10.1371\/journal.ppat.1012851","volume":"21","author":"SG Kanellopoulos","year":"2025","unstructured":"Kanellopoulos SG, Snelders E. Moving beyond multi-triazole to multi-fungicide resistance: broader selection of drug resistance in the human fungal pathogen Aspergillus fumigatus. PLoS Pathog. 2025;21(2):e1012851. https:\/\/doi.org\/10.1371\/journal.ppat.1012851.","journal-title":"PLoS Pathog"},{"issue":"Suppl 1","key":"993_CR21","doi-asserted-by":"publisher","first-page":"i9","DOI":"10.1093\/jac\/dkaf003","volume":"80","author":"MC Arendrup","year":"2025","unstructured":"Arendrup MC, Cordonnier C. Refractory versus resistant invasive aspergillosis. J Antimicrob Chemother. 2025;80(Suppl 1):i9\u201316. https:\/\/doi.org\/10.1093\/jac\/dkaf003.","journal-title":"J Antimicrob Chemother"},{"issue":"suppl_3","key":"993_CR22","doi-asserted-by":"publisher","first-page":"S436","DOI":"10.1093\/infdis\/jix210","volume":"216","author":"A Chowdhary","year":"2017","unstructured":"Chowdhary A, Sharma C, Meis JF. Azole-resistant aspergillosis: epidemiology, molecular mechanisms, and treatment. J Infect Dis. 2017;216(suppl_3):S436\u201344. https:\/\/doi.org\/10.1093\/infdis\/jix210.","journal-title":"J Infect Dis"},{"issue":"11","key":"993_CR23","doi-asserted-by":"publisher","first-page":"2979","DOI":"10.1093\/jac\/dku259","volume":"69","author":"A Chowdhary","year":"2014","unstructured":"Chowdhary A, Sharma C, Van Den Boom M, Yntema JB, Hagen F, Verweij PE, et al. Multi-azole-resistant Aspergillus fumigatus in the environment in Tanzania. J Antimicrob Chemother. 2014;69(11):2979\u201383. https:\/\/doi.org\/10.1093\/jac\/dku259.","journal-title":"J Antimicrob Chemother"},{"issue":"11","key":"993_CR24","doi-asserted-by":"publisher","first-page":"7148","DOI":"10.1128\/AAC.00887-15","volume":"59","author":"Y Chen","year":"2015","unstructured":"Chen Y, Wang H, Lu Z, Li P, Zhang Q, Jia T, et al. Emergence of TR46\/Y121F\/T289A in an Aspergillus fumigatus isolate from a Chinese patient. Antimicrob Agents Chemother. 2015;59(11):7148\u201350. https:\/\/doi.org\/10.1128\/AAC.00887-15.","journal-title":"Antimicrob Agents Chemother"},{"issue":"7","key":"993_CR25","doi-asserted-by":"publisher","first-page":"4331","DOI":"10.1128\/AAC.00127-15","volume":"59","author":"RA Lavergne","year":"2015","unstructured":"Lavergne RA, Morio F, Favennec L, Dominique S, Meis JF, Gargala G, et al. First description of azole-resistant Aspergillus fumigatus due to TR46\/Y121F\/T289A mutation in France. Antimicrob Agents Chemother. 2015;59(7):4331\u20135. https:\/\/doi.org\/10.1128\/AAC.00127-15.","journal-title":"Antimicrob Agents Chemother"},{"issue":"5","key":"993_CR26","doi-asserted-by":"publisher","first-page":"1522","DOI":"10.1093\/jac\/dku566","volume":"70","author":"J Steinmann","year":"2015","unstructured":"Steinmann J, Hamprecht A, Vehreschild MJGT, Cornely OA, Buchheidt D, Spiess B, et al. Emergence of azole-resistant invasive aspergillosis in HSCT recipients in Germany. J Antimicrob Chemother. 2015;70(5):1522\u20136. https:\/\/doi.org\/10.1093\/jac\/dku566.","journal-title":"J Antimicrob Chemother"},{"issue":"4","key":"993_CR27","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1016\/j.ijantimicag.2017.01.004","volume":"49","author":"CB Moore","year":"2017","unstructured":"Moore CB, Novak-Frazer L, Muldoon E, Dunn KW, Masania R, Richardson MD, et al. First isolation of the pan-azole-resistant Aspergillus fumigatus cyp51A TR46\/Y121F\/T289A mutant in a UK patient. Int J Antimicrob Agents. 2017;49(4):512\u20134. https:\/\/doi.org\/10.1016\/j.ijantimicag.2017.01.004.","journal-title":"Int J Antimicrob Agents"},{"issue":"8","key":"993_CR28","doi-asserted-by":"publisher","first-page":"e00270","DOI":"10.1128\/AAC.00270-17","volume":"61","author":"S R\u00f6\u00dfler","year":"2017","unstructured":"R\u00f6\u00dfler S, Bader O, St\u00f6lzel F, Sommer U, Spiess B, Geibel S, et al. Progressive dispersion of azole resistance in Aspergillus fumigatus: fatal invasive aspergillosis in a patient with acute myeloid leukemia infected with an A. fumigatus strain with a cyp51A TR46 Y121F M172I T289A allele. Antimicrob Agents Chemother. 2017;61(8):e00270. https:\/\/doi.org\/10.1128\/AAC.00270-17.","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"993_CR29","doi-asserted-by":"publisher","first-page":"122","DOI":"10.3390\/jof10020122","volume":"10","author":"L Pontes","year":"2024","unstructured":"Pontes L, Arai T, Gualtieri Beraquet CA, Giordano ALPL, Reichert-Lima F, da Luz EA, et al. Uncovering a novel cyp51A mutation and antifungal resistance in Aspergillus fumigatus through culture collection screening. J Fungi. 2024;10(2):122. https:\/\/doi.org\/10.3390\/jof10020122.","journal-title":"J Fungi"},{"issue":"2","key":"993_CR30","doi-asserted-by":"publisher","first-page":"291","DOI":"10.5578\/mb.69024","volume":"54","author":"\u00d6 Do\u011fan","year":"2020","unstructured":"Do\u011fan \u00d6, G\u00fclmez D, Ar\u0131kan AS. Phenotypic and genotypic evaluation of azole resistance in Aspergillus fumigatus Isolates from clinical and environmental specimens. Mikrobiyol Bul. 2020;54(2):291\u2013305. https:\/\/doi.org\/10.5578\/mb.69024.","journal-title":"Mikrobiyol Bul"},{"issue":"7","key":"993_CR31","doi-asserted-by":"publisher","first-page":"2356","DOI":"10.1021\/acsinfecdis.4c00203","volume":"10","author":"AC Bisen","year":"2024","unstructured":"Bisen AC, Sanap SN, Agrawal S, Biswas A, Mishra A, Verma SK, et al. Etiopathology, epidemiology, diagnosis, and treatment of fungal keratitis. ACS Infect Dis. 2024;10(7):2356\u201380. https:\/\/doi.org\/10.1021\/acsinfecdis.4c00203.","journal-title":"ACS Infect Dis"},{"issue":"5","key":"993_CR32","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.1016\/j.ophtha.2011.11.034","volume":"119","author":"S Parchand","year":"2012","unstructured":"Parchand S, Gupta A, Ram J, Gupta N, Chakrabarty A. Voriconazole for fungal cornaeal ulcers. Ophthalmology. 2012;119(5):1083. https:\/\/doi.org\/10.1016\/j.ophtha.2011.11.034.","journal-title":"Ophthalmology"},{"issue":"8","key":"993_CR33","doi-asserted-by":"publisher","first-page":"784","DOI":"10.3390\/jof9080784","volume":"9","author":"M Asadzadeh","year":"2023","unstructured":"Asadzadeh M, Alobaid K, Ahmad S, Mazloum S. First report of azole-resistant Aspergillus fumigatus with TR46\/Y121F\/T289A mutations in Kuwait and an update on their occurrence in the Middle East. J Fungi. 2023;9(8):784. https:\/\/doi.org\/10.3390\/jof9080784.","journal-title":"J Fungi"},{"issue":"5","key":"993_CR34","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1111\/myc.13064","volume":"63","author":"F Ahangarkani","year":"2020","unstructured":"Ahangarkani F, Puts Y, Nabili M, Khodavaisy S, Moazeni M, Salehi Z, et al. First azole-resistant Aspergillus fumigatus isolates with the environmental TR46 \/Y121F\/T289A mutation in Iran. Mycoses. 2020;63(5):430\u20136. https:\/\/doi.org\/10.1111\/myc.13064.","journal-title":"Mycoses"},{"key":"993_CR35","unstructured":"CLSI. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; 3rd ed.CLSI supplement M38. Wayne: Clinical and Laboratory Standards Institute; 2017."},{"issue":"3","key":"993_CR36","doi-asserted-by":"publisher","first-page":"e0095623","DOI":"10.1128\/spectrum.00956-23","volume":"11","author":"J Tan","year":"2023","unstructured":"Tan J, Zhang H, Sun Y, Gao L. Afu-Emi1 contributes to stress adaptation and voriconazole susceptibility in Aspergillus fumigatus. Microbiol Spectr. 2023;11(3):e0095623. https:\/\/doi.org\/10.1128\/spectrum.00956-23.","journal-title":"Microbiol Spectr"},{"key":"993_CR37","unstructured":"CLSI. Performance Standards for Antifungal Susceptibility Testing of Filamentous Fungi; 3rd ed. CLSI sup. M38M51S. CLSI. Wayne: Clinical and Laboratory Standards Institute; 2022."},{"key":"993_CR38","unstructured":"CLSI. Epidemiological Cutof Values for Antifungal Susceptibility Testing; 4th ed. CLSI supplement M57S. Wayne: Clinical and Laboratory Standards Institute; 2017."},{"key":"993_CR39","unstructured":"The European Committee on Antimicrobial Susceptibility Testing. Overview of antifungal ECOFFs and clinical breakpoints for yeasts, moulds and dermatophytes using the EUCAST E.Def 7.4, E.Def 9.4 and E.Def 11.0 procedures. Version 5.0. 2024. http:\/\/www.eucast.org."},{"key":"993_CR40","unstructured":"The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs. Version 11.0, valid from 12-02-2024. http:\/\/www.eucast.org."},{"issue":"4","key":"993_CR41","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1093\/cid\/cit320","volume":"57","author":"JWM Van Der Linden","year":"2013","unstructured":"Van Der Linden JWM, Camps SMT, Kampinga GA, Arends JPA, Debets-Ossenkopp YJ, Haas PJA, et al. Aspergillosis due to voriconazole highly resistant Aspergillus fumigatus and recovery of genetically related resistant isolates from domiciles. Clin Infect Dis. 2013;57(4):513\u201320. https:\/\/doi.org\/10.1093\/cid\/cit320.","journal-title":"Clin Infect Dis"},{"issue":"2","key":"993_CR42","doi-asserted-by":"publisher","first-page":"e01578","DOI":"10.1128\/AAC.01578-19","volume":"64","author":"H Fan","year":"2020","unstructured":"Fan H, Chen Y, Duan L, Zhao J, Qin C, Li H, et al. Comparison of two typing methods for characterization of azole resistance in Aspergillus fumigatus from potting soil samples in a Chinese hospital. Antimicrob Agents Chemother. 2020;64(2):e01578-e1619. https:\/\/doi.org\/10.1128\/AAC.01578-19.","journal-title":"Antimicrob Agents Chemother"},{"issue":"4","key":"993_CR43","doi-asserted-by":"publisher","first-page":"e0178223","DOI":"10.1128\/aem.01782-23","volume":"90","author":"BN Celia-Sanchez","year":"2024","unstructured":"Celia-Sanchez BN, Mangum B, G\u00f3mez Londo\u00f1o LF, Wang C, Shuman B, Brewer MT, et al. Pan-azole- and multi-fungicide-resistant Aspergillus fumigatus is widespread in the United States. Appl Environ Microbiol. 2024;90(4):e0178223. https:\/\/doi.org\/10.1128\/aem.01782-23.","journal-title":"Appl Environ Microbiol"},{"issue":"9","key":"993_CR44","doi-asserted-by":"publisher","first-page":"2759","DOI":"10.1093\/jac\/dkz258","volume":"74","author":"A Resendiz-Sharpe","year":"2019","unstructured":"Resendiz-Sharpe A, Mercier T, Lestrade PPA, Van Der Beek MT, Von Dem Borne PA, Cornelissen JJ, et al. Prevalence of voriconazole-resistant invasive aspergillosis and its impact on mortality in haematology patients. J Antimicrob Chemother. 2019;74(9):2759\u201366. https:\/\/doi.org\/10.1093\/jac\/dkz258.","journal-title":"J Antimicrob Chemother"},{"issue":"8","key":"993_CR45","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1016\/j.jiac.2016.01.015","volume":"22","author":"D Hagiwara","year":"2016","unstructured":"Hagiwara D, Takahashi H, Fujimoto M, Sugahara M, Misawa Y, Gonoi T, et al. Multi-azole resistant Aspergillus fumigatus harboring Cyp51A TR46\/Y121F\/T289A isolated in Japan. J Infect Chemother. 2016;22(8):577\u20139. https:\/\/doi.org\/10.1016\/j.jiac.2016.01.015.","journal-title":"J Infect Chemother"},{"issue":"4","key":"993_CR46","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1111\/myc.12306","volume":"58","author":"L Kredics","year":"2015","unstructured":"Kredics L, Narendran V, Shobana CS, V\u00e1gv\u00f6lgyi C, Manikandan P, Indo-Hungarian Fungal Keratitis Working Group. Filamentous fungal infections of the cornea: a global overview of epidemiology and drug sensitivity. Mycoses. 2015;58(4):243\u201360. https:\/\/doi.org\/10.1111\/myc.12306.","journal-title":"Mycoses"},{"key":"993_CR47","doi-asserted-by":"publisher","first-page":"2437","DOI":"10.2147\/OPTH.S301878","volume":"15","author":"G Saluja","year":"2021","unstructured":"Saluja G, Sharma N, Agarwal R, Sharma HP, Singhal D, Kumar Maharana P, et al. Comparison of safety and efficacy of intrastromal injections of voriconazole, amphotericin B and natamycin in cases of recalcitrant fungal keratitis: a randomized controlled trial. Clin Ophthalmol. 2021;15:2437\u201346. https:\/\/doi.org\/10.2147\/OPTH.S301878.","journal-title":"Clin Ophthalmol"},{"key":"993_CR48","doi-asserted-by":"publisher","DOI":"10.22034\/cmm.2024.345218.1529","author":"DP Singh","year":"2024","unstructured":"Singh DP, Verma RK, Yadav RK, Anant K, Das A. Candida utilis as an emerging rare cause of septicemia among neonates and children in Western Uttar Pradesh: a case series. Curr Med Mycol. 2024. https:\/\/doi.org\/10.22034\/cmm.2024.345218.1529.","journal-title":"Curr Med Mycol"},{"issue":"4","key":"993_CR49","doi-asserted-by":"publisher","first-page":"e38183","DOI":"10.7759\/cureus.38183","volume":"15","author":"B Dalyan Cilo","year":"2023","unstructured":"Dalyan Cilo B. Species distribution and antifungal susceptibilities of Candida species isolated from blood culture. Cureus. 2023;15(4):e38183. https:\/\/doi.org\/10.7759\/cureus.38183.","journal-title":"Cureus"},{"issue":"4","key":"993_CR50","doi-asserted-by":"publisher","first-page":"e01624","DOI":"10.1128\/JCM.01624-18","volume":"57","author":"YJ Kwon","year":"2019","unstructured":"Kwon YJ, Shin JH, Byun SA, Choi MJ, Won EJ, Lee D, et al. Candida auris clinical isolates from South Korea: identification, antifungal susceptibility, and genotyping. J Clin Microbiol. 2019;57(4):e01624-e1718. https:\/\/doi.org\/10.1128\/JCM.01624-18.","journal-title":"J Clin Microbiol"},{"issue":"12","key":"993_CR51","doi-asserted-by":"publisher","first-page":"e114834","DOI":"10.1371\/journal.pone.0114834","volume":"9","author":"EA Idelevich","year":"2014","unstructured":"Idelevich EA, Grunewald CM, W\u00fcllenweber J, Becker K. Rapid identification and susceptibility testing of Candida spp. from positive blood cultures by combination of direct MALDI-TOF mass spectrometry and direct inoculation of Vitek 2. PLoS ONE. 2014;9(12):e114834. https:\/\/doi.org\/10.1371\/journal.pone.0114834.","journal-title":"PLoS ONE"},{"issue":"9","key":"993_CR52","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1128\/JCM.00420-18","volume":"56","author":"M Vatanshenassan","year":"2018","unstructured":"Vatanshenassan M, Boekhout T, Lass-Fl\u00f6rl C, Lackner M, Schubert S, Kostrzewa M, et al. Proof of concept for MBT ASTRA, a rapid matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based method to detect caspofungin resistance in Candida albicans and Candida glabrata. J Clin Microbiol. 2018;56(9):10\u2013128. https:\/\/doi.org\/10.1128\/JCM.00420-18.","journal-title":"J Clin Microbiol"},{"key":"993_CR53","doi-asserted-by":"publisher","first-page":"720609","DOI":"10.3389\/fcimb.2021.720609","volume":"11","author":"C Durand","year":"2021","unstructured":"Durand C, Maubon D, Cornet M, Wang Y, Aldebert D, Garnaud C. Can we improve antifungal susceptibility testing? Front Cell Infect Microbiol. 2021;11:720609. https:\/\/doi.org\/10.3389\/fcimb.2021.720609.","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"993_CR54","doi-asserted-by":"publisher","first-page":"63","DOI":"10.3390\/jof7010063","volume":"7","author":"MA Knoll","year":"2021","unstructured":"Knoll MA, Ulmer H, Lass-Fl\u00f6rl C. Rapid antifungal susceptibility testing of yeasts and molds by MALDI-TOF MS: a systematic review and meta-analysis. J Fungi. 2021;7(1):63. https:\/\/doi.org\/10.3390\/jof7010063.","journal-title":"J Fungi"},{"issue":"6336","key":"993_CR55","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1126\/science.aam9321","volume":"356","author":"JS Gootenberg","year":"2017","unstructured":"Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, et al. Nucleic acid detection with CRISPR-Cas13a\/C2c2. Science. 2017;356(6336):438\u201342. https:\/\/doi.org\/10.1126\/science.aam9321.","journal-title":"Science"},{"issue":"6387","key":"993_CR56","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1126\/science.aas8836","volume":"360","author":"C Myhrvold","year":"2018","unstructured":"Myhrvold C, Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC, Durbin AF, et al. Field-deployable viral diagnostics using CRISPR-Cas13. Science. 2018;360(6387):444\u20138. https:\/\/doi.org\/10.1126\/science.aas8836.","journal-title":"Science"},{"issue":"6387","key":"993_CR57","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1126\/science.aaq0179","volume":"360","author":"JS Gootenberg","year":"2018","unstructured":"Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F. Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018;360(6387):439\u201344. https:\/\/doi.org\/10.1126\/science.aaq0179.","journal-title":"Science"},{"issue":"1","key":"993_CR58","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1186\/s12864-023-09691-w","volume":"24","author":"PL Yu","year":"2023","unstructured":"Yu PL, Fulton JC, Hudson OH, Huguet-Tapia JC, Brawner JT. Next-generation fungal identification using target enrichment and nanopore sequencing. BMC Genomics. 2023;24(1):581. https:\/\/doi.org\/10.1186\/s12864-023-09691-w.","journal-title":"BMC Genomics"},{"issue":"1","key":"993_CR59","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1186\/s12866-025-04132-y","volume":"25","author":"D Jing","year":"2025","unstructured":"Jing D, Jiang X, Ren X, Hao R, Su J, Li X. Clinical performance of nanopore targeted sequencing for diagnosing endophthalmitis. BMC Microbiol. 2025;25(1):403. https:\/\/doi.org\/10.1186\/s12866-025-04132-y.","journal-title":"BMC Microbiol"},{"key":"993_CR60","doi-asserted-by":"publisher","first-page":"1283094","DOI":"10.3389\/fcimb.2023.1283094","volume":"13","author":"Y Liu","year":"2023","unstructured":"Liu Y, Xu Y, Xu X, Chen X, Chen H, Zhang J, et al. Metagenomic identification of pathogens and antimicrobial-resistant genes in bacterial positive blood cultures by nanopore sequencing. Front Cell Infect Microbiol. 2023;13:1283094. https:\/\/doi.org\/10.3389\/fcimb.2023.1283094.","journal-title":"Front Cell Infect Microbiol"},{"issue":"3","key":"993_CR61","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1111\/lam.13516","volume":"73","author":"KL Chew","year":"2021","unstructured":"Chew KL, Octavia S, Jureen R, Lin RTP, Teo JWP. Targeted amplification and MinION nanopore sequencing of key azole and echinocandin resistance determinants of clinically relevant Candida spp. from blood culture bottles. Lett Appl Microbiol. 2021;73(3):286\u201393. https:\/\/doi.org\/10.1111\/lam.13516.","journal-title":"Lett Appl Microbiol"},{"issue":"4","key":"993_CR62","doi-asserted-by":"publisher","first-page":"90","DOI":"10.3390\/jof5040090","volume":"5","author":"A Arastehfar","year":"2019","unstructured":"Arastehfar A, Wickes BL, Ilkit M, Pincus DH, Daneshnia F, Pan W, et al. Identification of mycoses in developing countries. J Fungi. 2019;5(4):90. https:\/\/doi.org\/10.3390\/jof5040090.","journal-title":"J Fungi"}],"container-title":["Mycopathologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11046-025-00993-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11046-025-00993-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11046-025-00993-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T12:06:10Z","timestamp":1758974770000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11046-025-00993-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,11]]},"references-count":62,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["993"],"URL":"https:\/\/doi.org\/10.1007\/s11046-025-00993-z","relation":{},"ISSN":["0301-486X","1573-0832"],"issn-type":[{"type":"print","value":"0301-486X"},{"type":"electronic","value":"1573-0832"}],"subject":[],"published":{"date-parts":[[2025,9,11]]},"assertion":[{"value":"13 June 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 August 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 September 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors have no relevant financial or non-financial interests to disclose.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The strain Aftr46-001 used in this study was obtained from a clinical sample provided by the Ophthalmology Department of Jingzhou Central Hospital. This study has been reviewed and approved by the Ethics Committee of Jingzhou Central Hospital (Approval No.: 2025-192-01).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}}],"article-number":"83"}}