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In this study, we designed a novel loop-mediated isothermal amplification (LAMP) primer set capable of simultaneously detecting four\n                    <jats:italic>Madurella<\/jats:italic>\n                    species (\n                    <jats:italic>M. mycetomatis<\/jats:italic>\n                    ,\n                    <jats:italic>M. pseudomycetomatis<\/jats:italic>\n                    ,\n                    <jats:italic>M. tropicana,<\/jats:italic>\n                    and\n                    <jats:italic>M. fahalii<\/jats:italic>\n                    ). Genomic sequencing of\n                    <jats:italic>M. pseudomycetomatis<\/jats:italic>\n                    strain and comparative genome analysis revealed the candidate genes that were common among and specific to\n                    <jats:italic>Madurella<\/jats:italic>\n                    species. The 3 LAMP primer sets targeting these genes detected up to 1\u00a0pg of the genomic DNA of all 4\n                    <jats:italic>Madurella<\/jats:italic>\n                    species, exhibiting no cross-reactivity toward other pathogenic fungi. Among these, one primer set showing better reactivity was selected as a candidate used for diagnosis. Therefore, we developed novel primer sets which enabled the simultaneous detection of four\n                    <jats:italic>Madurella<\/jats:italic>\n                    species. Our present findings will lead to a faster and simpler diagnostic tool for eumycetoma detection, especially in rural clinical settings.\n                  <\/jats:p>","DOI":"10.1007\/s11046-025-01019-4","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T01:57:00Z","timestamp":1761703020000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Simultaneous Detection of Four Madurella Species Using Loop-Mediated Isothermal Amplification (LAMP) for Eumycetoma Diagnosis"],"prefix":"10.1007","volume":"190","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7449-2771","authenticated-orcid":false,"given":"Isato","family":"Yoshioka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4922-7321","authenticated-orcid":false,"given":"Ahmed Hassan","family":"Fahal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0560-2372","authenticated-orcid":false,"given":"Doudou","family":"Sow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9464-0911","authenticated-orcid":false,"given":"Satoshi","family":"Kaneko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6912-6648","authenticated-orcid":false,"given":"Yugo","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3442-308X","authenticated-orcid":false,"given":"Sayaka","family":"Ban","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9697-8470","authenticated-orcid":false,"given":"Takashi","family":"Yaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"1019_CR1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/S0035-9203(03)00009-9","volume":"98","author":"AH Fahal","year":"2004","unstructured":"Fahal AH. 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