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Roadkill provides a unique, underutilized opportunity to monitor\n                    <jats:italic>Sporothrix<\/jats:italic>\n                    circulation within human-impacted landscapes. We conducted a molecular survey via a triplex probe quantitative real-time PCR (qPCR) assay targeting pathogenic\n                    <jats:italic>Sporothrix<\/jats:italic>\n                    species in 81 roadkilled vertebrates (mammals, birds, and reptiles) collected along highways BR-376 and PR-445 traversing the Atlantic Forest in Paran\u00e1, Brazil (2017\u20132023). Genomic DNA from visceral organs (heart, liver, lung, and spleen) was screened for fungal DNA.\n                    <jats:italic>Sporothrix<\/jats:italic>\n                    DNA was detected in 13.6% (11\/81) of the samples.\n                    <jats:italic>Sporothrix schenckii<\/jats:italic>\n                    predominated, identified in wild mammals (\n                    <jats:italic>Leopardus guttulus<\/jats:italic>\n                    ,\n                    <jats:italic>Didelphis albiventris<\/jats:italic>\n                    , and\n                    <jats:italic>Lepus europaeus<\/jats:italic>\n                    ) and diverse birds (\n                    <jats:italic>Colaptes melanochloros<\/jats:italic>\n                    ,\n                    <jats:italic>Piaya cayana<\/jats:italic>\n                    , and\n                    <jats:italic>Selenidera maculirostris<\/jats:italic>\n                    ), indicating systemic exposure. Strikingly,\n                    <jats:italic>S. brasiliensis<\/jats:italic>\n                    was detected in avian hosts (\n                    <jats:italic>Columbina picui<\/jats:italic>\n                    ,\n                    <jats:italic>Crypturellus tataupa<\/jats:italic>\n                    ), challenging the thermal-exclusion hypothesis and implicating birds as potential aerial vectors. Furthermore,\n                    <jats:italic>S. globosa<\/jats:italic>\n                    was found in\n                    <jats:italic>Dasyprocta<\/jats:italic>\n                    spp. and was co-detected with\n                    <jats:italic>S. brasiliensis<\/jats:italic>\n                    in\n                    <jats:italic>Columbina picui<\/jats:italic>\n                    . Notably, a reptile (\n                    <jats:italic>Oxyrhopus<\/jats:italic>\n                    spp.) was positive for\n                    <jats:italic>S. globosa<\/jats:italic>\n                    and\n                    <jats:italic>S. schenckii<\/jats:italic>\n                    , expanding the known host spectrum, potentially via trophic transmission. Although distinguishing transient DNA carriage from active infection requires histopathological validation, these findings suggest that wildlife in fragmented corridors may function as reservoirs, biological amplifiers, or mechanical vectors rather than incidental hosts, reinforcing the blurring boundaries between sylvatic and synanthropic transmission cycles. In this context, integrating roadkill biosurveillance into a One Health framework is vital for tracking environmental pathogen loads and anticipating zoonotic spillover.\n                  <\/jats:p>","DOI":"10.1007\/s11046-026-01067-4","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:34:18Z","timestamp":1773282858000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular detection of medically relevant Sporothrix species in roadkilled wildlife in the Brazilian Atlantic forest"],"prefix":"10.1007","volume":"191","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5503-1566","authenticated-orcid":false,"given":"Steffanie Skau","family":"Amadei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5412-0425","authenticated-orcid":false,"given":"Julia","family":"Campos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4095-2167","authenticated-orcid":false,"given":"Andressa Maria Rorato Nascimento","family":"de Matos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2651-439X","authenticated-orcid":false,"given":"Keity Aparecida","family":"Spe\u00e7ato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0475-2010","authenticated-orcid":false,"given":"Eloiza Teles","family":"Caldart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5407-2582","authenticated-orcid":false,"given":"Ana Paula Frederico Rodrigues Loure","family":"Bracarense","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5622-1916","authenticated-orcid":false,"given":"Ferry","family":"Hagen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2971-7983","authenticated-orcid":false,"given":"Zoilo Pires","family":"de Camargo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0891-4563","authenticated-orcid":false,"given":"Anderson Messias","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,3,12]]},"reference":[{"issue":"2","key":"1067_CR1","doi-asserted-by":"publisher","DOI":"10.1590\/1676-0611-bn-2022-1454","volume":"23","author":"DF Gomes","year":"2023","unstructured":"Gomes DF, Bueno C, Pinna PH, Woitovicz-Cardoso M, Passos P. 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