{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:02:29Z","timestamp":1792670549594,"version":"4.5.0"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2015,7,3]],"date-time":"2015-07-03T00:00:00Z","timestamp":1435881600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Pharm Res"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1007\/s11095-015-1747-6","type":"journal-article","created":{"date-parts":[[2015,7,2]],"date-time":"2015-07-02T15:40:17Z","timestamp":1435851617000},"page":"3877-3885","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Histological Quantification of Gene Silencing by Intratracheal Administration of Dry Powdered Small-Interfering RNA\/Chitosan Complexes in the Murine Lung"],"prefix":"10.1007","volume":"32","author":[{"given":"Daisuke","family":"Ihara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noboru","family":"Hattori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasushi","family":"Horimasu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Masuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taku","family":"Nakashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Senoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Iwamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunori","family":"Fujitaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuoki","family":"Kohno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,7,3]]},"reference":[{"key":"1747_CR1","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1038\/35888","volume":"391","author":"A Fire","year":"1998","unstructured":"Fire A, Xu S, Montgomery MK, Kostas S, Driver SE, Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998;391:806\u201311.","journal-title":"Nature"},{"key":"1747_CR2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.addr.2011.02.006","volume":"64","author":"JK Lam","year":"2012","unstructured":"Lam JK, Liang W, Chan HK. Pulmonary delivery of therapeutic siRNA. Adv Drug Deliv Rev. 2012;64:1\u201315.","journal-title":"Adv Drug Deliv Rev"},{"key":"1747_CR3","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jconrel.2010.12.006","volume":"150","author":"H Okamoto","year":"2011","unstructured":"Okamoto H, Shiraki K, Yasuda R, Danjo K, Watanabe Y. Chitosan-interferon-\u03b2 gene complex powder for inhalation treatment of lung metastasis in mice. J Control Release. 2011;150:187\u201395.","journal-title":"J Control Release"},{"key":"1747_CR4","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jconrel.2008.11.018","volume":"134","author":"T Mizuno","year":"2009","unstructured":"Mizuno T, Mohri K, Nasu S, Danjo K, Okamoto H. Dual imaging of pulmonary delivery and gene expression of dry powder inhalant by fluorescence and bioluminescence. J Control Release. 2009;134:149\u201354.","journal-title":"J Control Release"},{"key":"1747_CR5","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.jconrel.2010.02.018","volume":"144","author":"K Mohri","year":"2010","unstructured":"Mohri K, Okuda T, Mori A, Danjo K, Okamoto H. Optimized pulmonary gene transfection in mice by spray-freeze dried powder inhalation. J Control Release. 2010;144:221\u20136.","journal-title":"J Control Release"},{"key":"1747_CR6","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.addr.2007.02.002","volume":"60","author":"H Okamoto","year":"2008","unstructured":"Okamoto H, Danjo K. Application of supercritical fluid to preparation of powders of high-molecular weight drugs for inhalation. Adv Drug Deliv Rev. 2008;60:433\u201346.","journal-title":"Adv Drug Deliv Rev"},{"key":"1747_CR7","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1002\/jps.10497","volume":"21","author":"H Todo","year":"2003","unstructured":"Todo H, Iida K, Okamoto H, Danjo K. Improvement of insulin absorption from intratracheally administrated dry powder prepared by supercritical carbon dioxide process. J Pharm Sci. 2003;21:2475\u201386.","journal-title":"J Pharm Sci"},{"key":"1747_CR8","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1023\/B:PHAM.0000048185.09483.e7","volume":"21","author":"N Jovanovi\u0107","year":"2004","unstructured":"Jovanovi\u0107 N, Bouchard A, Hofland GW, Witkamp GJ, Crommelin DJ, Jiskoot W. Stabilization of proteins in dry powder formulations using supercritical fluid technology. Pharm Res. 2004;21:1955\u201369.","journal-title":"Pharm Res"},{"key":"1747_CR9","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1002\/jps.10285","volume":"92","author":"H Okamoto","year":"2003","unstructured":"Okamoto H, Nishida S, Todo H, Sakakura Y, Iida K, Danjo K. Pulmonary gene delivery by chitosan-pDNA complex powder prepared with supercritical carbon dioxide. J Pharm Sci. 2003;92:371\u201380.","journal-title":"J Pharm Sci"},{"key":"1747_CR10","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1248\/bpb.b13-00167","volume":"36","author":"T Okuda","year":"2013","unstructured":"Okuda T, Kito D, Oiwa A, Fukushima M, Hira D, Okamoto H. Gene silencing in a mouse lung metastasis model by an inhalable dry small interfering RNA powder prepared using the supercritical carbon dioxide technique. Biol Pharm Bull. 2013;36:1183\u201391.","journal-title":"Biol Pharm Bull"},{"key":"1747_CR11","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jconrel.2012.06.005","volume":"162","author":"Y Luo","year":"2012","unstructured":"Luo Y, Zhai X, Ma C, Sun P, Fu Z, Liu W, et al. An inhalable \u03b22-adrenoceptor ligand-directed guanidinylated chitosan carrier for targeted delivery of siRNA to lung. J Control Release. 2012;162:28\u201336.","journal-title":"J Control Release"},{"key":"1747_CR12","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1021\/mp900107v","volume":"6","author":"OM Merkel","year":"2009","unstructured":"Merkel OM, Beyerle A, Librizzi D, Pfestroff A, Behr TM, Sproat B, et al. Nonviral siRNA delivery to the lung: investigation of PEG-PEI polyplexes and their in vivo performance. Mol Pharm. 2009;6:1246\u201360.","journal-title":"Mol Pharm"},{"key":"1747_CR13","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1016\/S0002-9440(10)61240-0","volume":"167","author":"M Perl","year":"2005","unstructured":"Perl M, Chung CS, Lomas-Neira J, Rachel TM, Biffl WL, Cioffi WG, et al. Silencing of Fas, but not caspase-8, in lung epithelial cells ameliorates pulmonary apoptosis, inflammation, and neutrophil influx after hemorrhagic shock and sepsis. Am J Pathol. 2005;167:1545\u201359.","journal-title":"Am J Pathol"},{"key":"1747_CR14","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.ymthe.2006.04.010","volume":"14","author":"KA Howard","year":"2006","unstructured":"Howard KA, Rahbek UL, Liu X, Damgaard CK, Glud SZ, Andersen M\u00d8, et al. RNA interference in vitro and in vivo using a novel chitosan\/siRNA nanoparticle system. Mol Ther. 2006;14:476\u201384.","journal-title":"Mol Ther"},{"key":"1747_CR15","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1258\/ebm.2011.011280","volume":"237","author":"XS Dong","year":"2012","unstructured":"Dong XS, Hu XB, Liu W, Sun YQ, Liu Z. Effects of RNA interference-induced Smad3 gene silencing on pulmonary fibrosis caused by paraquat in mice. Exp Biol Med. 2012;237:548\u201355.","journal-title":"Exp Biol Med"},{"key":"1747_CR16","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1089\/hum.2012.012","volume":"23","author":"CJ Wu","year":"2012","unstructured":"Wu CJ, Huang WC, Chen LC, Shen CR, Kuo ML. Pseudotyped adeno-associated virus 2\/9-delivered CCL11 shRNA alleviates lung inflammation in an allergen-sensitized mouse model. Hum Gene Ther. 2012;23:1156\u201365.","journal-title":"Hum Gene Ther"},{"key":"1747_CR17","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1208\/s12248-009-9140-1","volume":"11","author":"SY Wu","year":"2009","unstructured":"Wu SY, McMillan NA. Lipidic systems for in vivo siRNA delivery. AAPS J. 2009;11:639\u201352.","journal-title":"AAPS J"},{"key":"1747_CR18","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1023\/A:1007504613351","volume":"17","author":"S Dokka","year":"2000","unstructured":"Dokka S, Toledo D, Shi XG, Castranova V, Rojanasakul Y. Oxygen radical-mediated pulmonary toxicity induced by some cationic liposomes. Pharm Res. 2000;17:521\u20135.","journal-title":"Pharm Res"},{"key":"1747_CR19","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1021\/bc070077d","volume":"18","author":"SA Moschos","year":"2007","unstructured":"Moschos SA, Jones SW, Perry MM, Williams AE, Erjefalt JS, Turner JJ, et al. Lung delivery studies using siRNA conjugated to TAT(48-60) and penetratin reveal peptide induced reduction in gene expression and induction of innate immunity. Bioconjug Chem. 2007;18:1450\u20139.","journal-title":"Bioconjug Chem"},{"key":"1747_CR20","doi-asserted-by":"crossref","first-page":"R60","DOI":"10.1186\/ar2973","volume":"12","author":"JC Wang","year":"2010","unstructured":"Wang JC, Lai SL, Guo XJ, Zhang XF, de Crombrugghe B, Sonnylal S, et al. Attenuation of fibrosis in vitro and in vivo with SPARC siRNA. Arthritis Res Ther. 2010;12:R60.","journal-title":"Arthritis Res Ther"},{"key":"1747_CR21","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/nm1164","volume":"11","author":"V Bitko","year":"2005","unstructured":"Bitko V, Musiyenko A, Shulyayeva O, Barik S. Inhibition of respiratory viruses by nasally administered siRNA. Nat Med. 2005;11:50\u20135.","journal-title":"Nat Med"},{"key":"1747_CR22","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1021\/mp900107v","volume":"6","author":"OM Merkel","year":"2009","unstructured":"Merkel OM, Beyerle A, Librizzi D, Pfestroff A, Behr TM, Sproat B, et al. Nonviral siRNA delivery to the lung: investigation of PEG-PEI polyplexes and their in vivo performance. Mol Pharm. 2009;6:1246\u201360.","journal-title":"Mol Pharm"},{"key":"1747_CR23","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jconrel.2010.12.017","volume":"151","author":"A Beyerle","year":"2011","unstructured":"Beyerle A, Braun A, Merkel O, Koch F, Kissel T, Stoeger T. Comparative in vivo study of poly(ethylene imine)\/siRNA complexes for pulmonary delivery in mice. J Control Release. 2011;151:51\u20136.","journal-title":"J Control Release"},{"key":"1747_CR24","doi-asserted-by":"crossref","first-page":"5844","DOI":"10.1016\/j.biomaterials.2009.07.017","volume":"30","author":"HL Jiang","year":"2009","unstructured":"Jiang HL, Xu CX, Kim YK, Arote R, Jere D, Lim HT, et al. The suppression of lung tumorigenesis by aerosol-delivered folate-chitosan-graft-polyethylenimine\/Akt1 shRNA complexes through the Akt signaling pathway. Biomaterials. 2009;30:5844\u201352.","journal-title":"Biomaterials"},{"key":"1747_CR25","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.addr.2009.08.004","volume":"62","author":"S Mao","year":"2009","unstructured":"Mao S, Sun W, Kisel T. Chitosan-based formulations for delivery of DNA and siRNA. Adv Drug Deliv Rev. 2009;62:12\u201327.","journal-title":"Adv Drug Deliv Rev"},{"key":"1747_CR26","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.1016\/j.biomaterials.2004.07.008","volume":"26","author":"MK Lee","year":"2005","unstructured":"Lee MK, Chun SK, Choi WJ, Kim JK, Choi SH, Kim A, et al. The use of chitosan as a condensing agent to enhance emulsion-mediated gene transfer. Biomaterials. 2005;26:2147\u201356.","journal-title":"Biomaterials"},{"key":"1747_CR27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijpharm.2010.08.022","volume":"399","author":"WE Rudzinski","year":"2010","unstructured":"Rudzinski WE, Aminabhavi TM. Chitosan as a carrier for targeted delivery of small interfering RNA. Int J Pharm. 2010;399:1\u201311.","journal-title":"Int J Pharm"}],"container-title":["Pharmaceutical Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11095-015-1747-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s11095-015-1747-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11095-015-1747-6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T22:59:13Z","timestamp":1566946753000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s11095-015-1747-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,3]]},"references-count":27,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["1747"],"URL":"https:\/\/doi.org\/10.1007\/s11095-015-1747-6","relation":{},"ISSN":["0724-8741","1573-904X"],"issn-type":[{"value":"0724-8741","type":"print"},{"value":"1573-904X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,3]]}}}