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DHA requires access across the blood\u2013brain barrier (BBB) to enter the brain, and iron has been shown to affect the expression and function of a number of BBB transporters. Therefore, this study aimed to assess the effect of iron on the expression and function of fatty acid binding protein 5 (FABP5) and fatty acid transport protein 1 (FATP1), both which mediate brain endothelial cell trafficking of DHA.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The mRNA and protein levels of FABP5 and FATP1 in human cerebral microvascular endothelial (hCMEC\/D3) cells was assessed by RT-qPCR and Western blot, respectively following ferric ammonium citrate (FAC) treatment (up to 750\u00a0\u00b5M, 72\u00a0h). The function of FABP5 and FATP1 was assessed via uptake and efflux of radiolabelled <jats:sup>3<\/jats:sup>H-oleic acid and <jats:sup>14<\/jats:sup>C-DHA.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>FAC (500\u00a0\u00b5M, 72\u00a0h) had no impact on the expression of FABP5 at the protein and mRNA level in hCMEC\/D3 cells, which was associated with a lack of effect on the uptake of <jats:sup>14<\/jats:sup>C-DHA. FAC led to a 19.7% reduction in FATP1 protein abundance in hCMEC\/D3 cells with no impact on mRNA levels, and this was associated with up to a 32.6% reduction in efflux of <jats:sup>14<\/jats:sup>C-DHA.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>These studies demonstrate a role of iron in down-regulating FATP1 protein abundance and function at the BBB, which may have implications on fatty acid access to the brain.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1007\/s11095-024-03743-w","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T02:01:50Z","timestamp":1721786510000},"page":"1631-1648","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Iron Reduces the Trafficking of Fatty Acids from Human Immortalised Brain Microvascular Endothelial Cells Through Modulation of Fatty Acid Transport Protein 1 (FATP1\/SLC27A1)"],"prefix":"10.1007","volume":"41","author":[{"given":"Showmika T.","family":"Supti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liam M.","family":"Koehn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie A.","family":"Newman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0983-7152","authenticated-orcid":false,"given":"Joseph A.","family":"Nicolazzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,24]]},"reference":[{"issue":"6","key":"3743_CR1","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1007\/BF02536067","volume":"26","author":"M S\u00f6derberg","year":"1991","unstructured":"S\u00f6derberg M, Edlund C, Kristensson K, Dallner G. 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