{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:07:41Z","timestamp":1790104061450,"version":"4.0.1"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/100014943","name":"Sarepta Therapeutics","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014943","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Pharm Res"],"DOI":"10.1007\/s11095-026-04187-0","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T18:46:39Z","timestamp":1790102799000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanistic Insights into Poloxamer 188\u2013Mediated Stabilization of AAV8 Capsids Using Coarse-Grained Molecular Dynamics"],"prefix":"10.1007","author":[{"given":"Leila","family":"Sharifi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jahanbakhsh","family":"Ghasemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo","family":"Santisi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivangi","family":"Naik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willow","family":"DiLuzio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arani","family":"Chanda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1286-0871","authenticated-orcid":false,"given":"Bodhisattwa","family":"Chaudhuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"4187_CR1","doi-asserted-by":"publisher","first-page":"105834","DOI":"10.1016\/j.ebiom.2025.105834","volume":"118","author":"G Geng","year":"2025","unstructured":"Geng G, Xu Y, Hu Z, Wang H, Chen X, Yuan W, et al. Viral and non-viral vectors in gene therapy: current state and clinical perspectives. EBioMedicine. 2025;118:105834. https:\/\/doi.org\/10.1016\/j.ebiom.2025.105834.","journal-title":"EBioMedicine"},{"key":"4187_CR2","doi-asserted-by":"publisher","DOI":"10.1038\/S41392-024-01780-W","author":"JH Wang","year":"2024","unstructured":"Wang JH, Gessler DJ, Zhan W, Gallagher TL, Gao G. Adeno-associated virus as a delivery vector for gene therapy of human diseases. Signal Transduct Target Ther. 2024. https:\/\/doi.org\/10.1038\/S41392-024-01780-W.","journal-title":"Signal Transduct Target Ther"},{"issue":"17","key":"4187_CR3","doi-asserted-by":"publisher","first-page":"e70056","DOI":"10.1111\/JCMM.70056;WGROUP:STRING:PUBLICATION","volume":"28","author":"B Cetin","year":"2024","unstructured":"Cetin B, Erendor F, Eksi YE, Sanlioglu AD, Sanlioglu S. Gene and cell therapy of human genetic diseases: recent advances and future directions. J Cell Mol Med. 2024;28(17):e70056. https:\/\/doi.org\/10.1111\/JCMM.70056;WGROUP:STRING:PUBLICATION.","journal-title":"J Cell Mol Med"},{"issue":"7","key":"4187_CR4","doi-asserted-by":"publisher","first-page":"2609","DOI":"10.1016\/J.XPHS.2021.03.024","volume":"110","author":"A Srivastava","year":"2021","unstructured":"Srivastava A, Mallela KMG, Deorkar N, Brophy G. Manufacturing challenges and rational formulation development for AAV viral vectors. J Pharm Sci. 2021;110(7):2609\u201324. https:\/\/doi.org\/10.1016\/J.XPHS.2021.03.024.","journal-title":"J Pharm Sci"},{"issue":"1","key":"4187_CR5","doi-asserted-by":"publisher","first-page":"27685","DOI":"10.1038\/s41598-024-79369-0","volume":"14","author":"J Lengler","year":"2024","unstructured":"Lengler J, Gavrila M, Brandis J, Palavra K, Dieringer F, Unterthurner S, et al. Crucial aspects for maintaining rAAV stability. Sci Rep. 2024;14(1):27685. https:\/\/doi.org\/10.1038\/s41598-024-79369-0.","journal-title":"Sci Rep"},{"issue":"10","key":"4187_CR6","doi-asserted-by":"publisher","first-page":"1268","DOI":"10.1016\/j.tibtech.2023.04.002","volume":"41","author":"Z Jiang","year":"2023","unstructured":"Jiang Z, Dalby PA. Challenges in scaling up AAV-based gene therapy manufacturing. Trends Biotechnol. 2023;41(10):1268\u201381. https:\/\/doi.org\/10.1016\/j.tibtech.2023.04.002.","journal-title":"Trends Biotechnol"},{"issue":"9","key":"4187_CR7","doi-asserted-by":"publisher","first-page":"2967","DOI":"10.1016\/j.xphs.2024.05.034","volume":"113","author":"A Rodriguez","year":"2024","unstructured":"Rodriguez A, Jalimarada-Shivakumar S, Banazadeh A, Afroz S, Ali A, Deng K, et al. Insight into the degradation pathways of an AAV9. J Pharm Sci. 2024;113(9):2967\u201373. https:\/\/doi.org\/10.1016\/j.xphs.2024.05.034.","journal-title":"J Pharm Sci"},{"issue":"7","key":"4187_CR8","doi-asserted-by":"publisher","first-page":"1868","DOI":"10.1016\/j.xphs.2022.03.018","volume":"111","author":"JS Bee","year":"2022","unstructured":"Bee JS, Zhang Y, Finkner S, O\u2019Berry K, Kaushal A, Phillippi MK, et al. Mechanistic studies and formulation mitigations of Adeno-associated virus capsid rupture during freezing and thawing: mechanisms of freeze\/thaw induced AAV rupture. J Pharm Sci. 2022;111(7):1868\u201378. https:\/\/doi.org\/10.1016\/j.xphs.2022.03.018.","journal-title":"J Pharm Sci"},{"key":"4187_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/J.EJPB.2023.07.002","volume":"190","author":"P Grossen","year":"2023","unstructured":"Grossen P, Koukelli IS, van Haasteren J, Machado AHE, D\u00fcrr C. The ice age \u2013 A review on formulation of Adeno-associated virus therapeutics. Eur J Pharm Biopharm. 2023;190:1\u201323. https:\/\/doi.org\/10.1016\/J.EJPB.2023.07.002.","journal-title":"Eur J Pharm Biopharm."},{"issue":"1","key":"4187_CR10","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1186\/s12896-024-00853-6","volume":"24","author":"SP Thomas","year":"2024","unstructured":"Thomas SP, Spinelli MM, Rghei AD, Lopes JA, Zielinska N, McLeod BM, et al. Analysis of the impact of pluronic acid on the thermal stability and infectivity of AAV6.2FF. BMC Biotechnol. 2024;24(1):22. https:\/\/doi.org\/10.1186\/s12896-024-00853-6.","journal-title":"BMC Biotechnol"},{"issue":"8","key":"4187_CR11","doi-asserted-by":"publisher","first-page":"2190","DOI":"10.1016\/j.xphs.2023.05.009","volume":"112","author":"C Hiemenz","year":"2023","unstructured":"Hiemenz C, Pacios-Michelena A, Helbig C, Vezo\u010dnik V, Strebl M, Nikels F, et al. Characterization of virus particles and submicron-sized particulate impurities in recombinant adeno-associated virus drug product. J Pharm Sci. 2023;112(8):2190\u2013202. https:\/\/doi.org\/10.1016\/j.xphs.2023.05.009.","journal-title":"J Pharm Sci"},{"issue":"7","key":"4187_CR12","doi-asserted-by":"publisher","first-page":"103825","DOI":"10.1016\/j.xphs.2025.103825","volume":"114","author":"C Patrick","year":"2025","unstructured":"Patrick C, Petroff M, Plegaria J, Razinkov V, Padmakumar S, Mallela KMG. Polysorbates and poloxamer rescue filter-induced serotype-dependent loss of AAVs. J Pharm Sci. 2025;114(7):103825. https:\/\/doi.org\/10.1016\/j.xphs.2025.103825.","journal-title":"J Pharm Sci"},{"issue":"2","key":"4187_CR13","doi-asserted-by":"publisher","first-page":"201725","DOI":"10.1016\/j.omta.2026.201725","volume":"34","author":"Y Pan","year":"2026","unstructured":"Pan Y, Zhong Y, Chen H, et al. Engineering novel AAV capsids by broadly attenuated and subsequent muscle-specific tropism in mice and NHPs. Mol Ther Adv. 2026;34(2):201725. https:\/\/doi.org\/10.1016\/j.omta.2026.201725.","journal-title":"Mol Ther Adv"},{"issue":"2","key":"4187_CR14","doi-asserted-by":"publisher","first-page":"73","DOI":"10.3390\/membranes16020073","volume":"16","author":"S Cardoso","year":"2026","unstructured":"Cardoso S, Bollmann F, Tappe A. Optimization of tangential flow filtration for high-yield, scalable downstream processing of adeno-associated virus. Membranes (Basel). 2026;16(2):73. https:\/\/doi.org\/10.3390\/membranes16020073.","journal-title":"Membranes (Basel)"},{"issue":"7","key":"4187_CR15","doi-asserted-by":"publisher","first-page":"1711","DOI":"10.1016\/j.xphs.2024.03.024","volume":"113","author":"M Som","year":"2024","unstructured":"Som M, Gikanga B, Kanapuram V, Yadav S. Drug product formulation and fill\/finish manufacturing process considerations for AAV-based genomic medicines. J Pharm Sci. 2024;113(7):1711\u201325. https:\/\/doi.org\/10.1016\/j.xphs.2024.03.024.","journal-title":"J Pharm Sci"},{"issue":"8","key":"4187_CR16","doi-asserted-by":"publisher","first-page":"2288","DOI":"10.1016\/j.xphs.2022.03.004","volume":"111","author":"R Rieser","year":"2022","unstructured":"Rieser R, Menzen T, Biel M, Michalakis S, Winter G. Systematic studies on stabilization of AAV vector formulations by lyophilization. J Pharm Sci. 2022;111(8):2288\u201398. https:\/\/doi.org\/10.1016\/j.xphs.2022.03.004.","journal-title":"J Pharm Sci"},{"key":"4187_CR17","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1186\/s41120-025-00131-5","volume":"11","author":"I Skaripa-Koukelli","year":"2025","unstructured":"Skaripa-Koukelli I, Schmidt O, Ketterer C, Lardenoije R, Qutaishat S, Machado AHE, et al. Optimizing laboratory practices for recombinant adeno-associated viral vectors: impact of stress factors on vector stability. AAPS Open. 2025;11:28. https:\/\/doi.org\/10.1186\/s41120-025-00131-5.","journal-title":"AAPS Open"},{"key":"4187_CR18","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.omtm.2019.11.005","volume":"17","author":"MI Patr\u00edcio","year":"2020","unstructured":"Patr\u00edcio MI, Cox CI, Blue C, Barnard AR, Martinez-Fernandez de la Camara C, MacLaren RE. Inclusion of PF68 surfactant improves stability of rAAV titer when passed through a surgical device used in retinal gene therapy. Mol Ther Methods Clin Dev. 2020;17:99\u2013106. https:\/\/doi.org\/10.1016\/j.omtm.2019.11.005.","journal-title":"Mol Ther Methods Clin Dev"},{"key":"4187_CR19","doi-asserted-by":"publisher","first-page":"114598","DOI":"10.1016\/j.jviromet.2022.114598","volume":"309","author":"A Chan","year":"2022","unstructured":"Chan A, Maturana CJ, Engel EA. Optimized formulation buffer preserves adeno-associated virus-9 infectivity after 4 \u00b0C storage and freeze\/thawing cycling. J Virol Methods. 2022;309:114598. https:\/\/doi.org\/10.1016\/j.jviromet.2022.114598.","journal-title":"J Virol Methods"},{"key":"4187_CR20","doi-asserted-by":"publisher","first-page":"121464","DOI":"10.1016\/j.ijpharm.2022.121464","volume":"615","author":"Y Xu","year":"2022","unstructured":"Xu Y, Jiang B, Samai P, Tank SM, Shameem M, Liu D. Genome DNA leakage of adeno-associated virus under freeze\u2013thaw stress. Int J Pharm. 2022;615:121464. https:\/\/doi.org\/10.1016\/j.ijpharm.2022.121464.","journal-title":"Int J Pharm"},{"issue":"2","key":"4187_CR21","doi-asserted-by":"publisher","first-page":"974","DOI":"10.1021\/ACS.JCIM.9B00883\/SUPPL_FILE\/CI9B00883_SI_002.ZIP","volume":"60","author":"M Mart\u00ednez","year":"2020","unstructured":"Mart\u00ednez M, Cooper CD, Poma AB, Guzman HV. Free energies of the disassembly of viral capsids from a multiscale molecular simulation approach. J Chem Inf Model. 2020;60(2):974\u201381. https:\/\/doi.org\/10.1021\/ACS.JCIM.9B00883\/SUPPL_FILE\/CI9B00883_SI_002.ZIP.","journal-title":"J Chem Inf Model"},{"key":"4187_CR22","doi-asserted-by":"publisher","DOI":"10.1016\/J.IJPHARM.2024.123985","author":"T Duran","year":"2024","unstructured":"Duran T, Naik S, Sharifi L, DiLuzio WR, Chanda A, Chaudhuri B. Studying the ssDNA loaded adeno-associated virus aggregation using coarse-grained molecular dynamics simulations. Int J Pharm. 2024. https:\/\/doi.org\/10.1016\/J.IJPHARM.2024.123985.","journal-title":"Int J Pharm"},{"key":"4187_CR23","doi-asserted-by":"publisher","first-page":"125867","DOI":"10.1016\/J.IJPHARM.2025.125867","volume":"681","author":"L Sharifi","year":"2025","unstructured":"Sharifi L, Ghasemi J, Duran T, Naik S, DiLuzio W, Sharifi A, et al. Impact of salt on AAV8 capsid aggregation with single-stranded DNA: insights from coarse-grained molecular dynamics simulations. Int J Pharm. 2025;681:125867. https:\/\/doi.org\/10.1016\/J.IJPHARM.2025.125867.","journal-title":"Int J Pharm"},{"issue":"9","key":"4187_CR24","doi-asserted-by":"publisher","first-page":"4587","DOI":"10.1021\/ACS.MOLPHARMACEUT.3C00312","volume":"20","author":"J Li","year":"2023","unstructured":"Li J, Sonje J, Suryanarayanan R. Role of poloxamer 188 in preventing ice-surface-induced protein destabilization during freeze\u2013thawing. Mol Pharm. 2023;20(9):4587\u201396. https:\/\/doi.org\/10.1021\/ACS.MOLPHARMACEUT.3C00312.","journal-title":"Mol Pharm"},{"issue":"9","key":"4187_CR25","doi-asserted-by":"publisher","first-page":"4486","DOI":"10.1021\/ACS.JCTC.5B00513\/SUPPL_FILE\/CT5B00513_SI_001.PDF","volume":"11","author":"Y Qi","year":"2015","unstructured":"Qi Y, Ing\u00f3lfsson HI, Cheng X, Lee J, Marrink SJ, Im W. CHARMM-GUI martini maker for coarse-grained simulations with the martini force field. J Chem Theory Comput. 2015;11(9):4486\u201394. https:\/\/doi.org\/10.1021\/ACS.JCTC.5B00513\/SUPPL_FILE\/CT5B00513_SI_001.PDF.","journal-title":"J Chem Theory Comput"},{"issue":"1","key":"4187_CR26","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1021\/CT300646G\/SUPPL_FILE\/CT300646G_SI_001.PDF","volume":"9","author":"DH De Jong","year":"2013","unstructured":"De Jong DH, Singh G, Bennett WFD, Arnarez C, Wassenaar TA, Sch\u00e4fer LV, et al. Improved parameters for the martini coarse-grained protein force field. J Chem Theory Comput. 2013;9(1):687\u201397. https:\/\/doi.org\/10.1021\/CT300646G\/SUPPL_FILE\/CT300646G_SI_001.PDF.","journal-title":"J Chem Theory Comput"},{"key":"4187_CR27","doi-asserted-by":"publisher","first-page":"3932","DOI":"10.1021\/acs.jctc.5b00286","volume":"11","author":"JJ Uusitalo","year":"2015","unstructured":"Uusitalo JJ, Ingolfsson HI, Akhshi P, Tieleman DP, Marrink SJ. Martini coarse-grained force field: extension to DNA. J Chem Theory Comput. 2015;11:3932\u201345.","journal-title":"J Chem Theory Comput"},{"issue":"2","key":"4187_CR28","doi-asserted-by":"publisher","first-page":"5","DOI":"10.5501\/WJV.V9.I2.5","volume":"9","author":"MK Parvez","year":"2020","unstructured":"Parvez MK. Geometric architecture of viruses. World J Virol. 2020;9(2):5. https:\/\/doi.org\/10.5501\/WJV.V9.I2.5.","journal-title":"World J Virol"},{"key":"4187_CR29","doi-asserted-by":"publisher","first-page":"462353","DOI":"10.1016\/J.CHROMA.2021.462353","volume":"1652","author":"W Chen","year":"2021","unstructured":"Chen W, Ross A, Steinhuber B, Hoffmann G, Oltra NS, Ravuri SKK, et al. The development and qualification of liquid adsorption chromatography for poloxamer 188 characterization. J Chromatogr A. 2021;1652:462353. https:\/\/doi.org\/10.1016\/J.CHROMA.2021.462353.","journal-title":"J Chromatogr A"},{"issue":"34","key":"4187_CR30","doi-asserted-by":"publisher","first-page":"21224","DOI":"10.1021\/acs.jpcc.9b04099","volume":"123","author":"G P\u00e9rez-S\u00e1nchez","year":"2019","unstructured":"P\u00e9rez-S\u00e1nchez G, Vicente FA, Schaeffer N, Cardoso IS, Ventura SPM, Jorge M, et al. Rationalizing the phase behavior of triblock copolymers through experiments and molecular simulations. J Phys Chem C. 2019;123(34):21224\u201336. https:\/\/doi.org\/10.1021\/acs.jpcc.9b04099.","journal-title":"J Phys Chem C"},{"issue":"16","key":"4187_CR31","doi-asserted-by":"publisher","first-page":"1701","DOI":"10.1002\/JCC.20291;JOURNAL:JOURNAL:1096987X;WGROUP:STRING:PUBLICATION","volume":"26","author":"D Van Der Spoel","year":"2005","unstructured":"Van Der Spoel D, Lindahl E, Hess B, Groenhof G, Mark AE, Berendsen HJC. GROMACS: fast, flexible, and free. J Comput Chem. 2005;26(16):1701\u201318. https:\/\/doi.org\/10.1002\/JCC.20291;JOURNAL:JOURNAL:1096987X;WGROUP:STRING:PUBLICATION.","journal-title":"J Comput Chem"},{"issue":"27","key":"4187_CR32","doi-asserted-by":"publisher","first-page":"7812","DOI":"10.1021\/jp071097f","volume":"111","author":"SJ Marrink","year":"2007","unstructured":"Marrink SJ, Risselada HJ, Yefimov S, Tieleman DP, Vries AH. The MARTINI force field: coarse grained model for biomolecular simulations. J Phys Chem B. 2007;111(27):7812\u201324. https:\/\/doi.org\/10.1021\/jp071097f.","journal-title":"J Phys Chem B"},{"issue":"6","key":"4187_CR33","doi-asserted-by":"publisher","first-page":"1000810","DOI":"10.1371\/journal.pcbi.1000810","volume":"6","author":"SO Yesylevskyy","year":"2010","unstructured":"Yesylevskyy SO, Sch\u00e4fer LV, Sengupta D, Marrink SJ. Polarizable water model for the coarse-grained MARTINI force field. PLoS Comput Biol. 2010;6(6):1000810. https:\/\/doi.org\/10.1371\/journal.pcbi.1000810.","journal-title":"PLoS Comput Biol"},{"key":"4187_CR34","doi-asserted-by":"publisher","unstructured":"Bussi G, Donadio D, Parrinello M. Canonical sampling through velocity rescaling. J Chem Phys. 2007;126(1). https:\/\/doi.org\/10.1063\/1.2408420\/186581.","DOI":"10.1063\/1.2408420\/186581"},{"issue":"8","key":"4187_CR35","doi-asserted-by":"publisher","first-page":"3684","DOI":"10.1063\/1.448118","volume":"81","author":"HJC Berendsen","year":"1984","unstructured":"Berendsen HJC, Postma JPM, Gunsteren WF, DiNola A, Haak JR. Molecular dynamics with coupling to an external bath. J Chem Phys. 1984;81(8):3684\u201390. https:\/\/doi.org\/10.1063\/1.448118.","journal-title":"J Chem Phys"},{"issue":"10","key":"4187_CR36","doi-asserted-by":"publisher","first-page":"2319","DOI":"10.1002\/jcc.21787","volume":"32","author":"N Michaud\u2010Agrawal","year":"2011","unstructured":"Michaud\u2010Agrawal N, Denning EJ, Woolf TB, Beckstein O. MDAnalysis: a toolkit for the analysis of molecular dynamics simulations. J Comput Chem. 2011;32(10):2319\u201327. https:\/\/doi.org\/10.1002\/jcc.21787.","journal-title":"J Comput Chem"},{"issue":"3","key":"4187_CR37","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1016\/j.str.2005.11.014","volume":"14","author":"PL Freddolino","year":"2006","unstructured":"Freddolino PL, Arkhipov AS, Larson SB, McPherson A, Schulten K. Molecular dynamics simulations of the complete satellite tobacco mosaic virus. Structure. 2006;14(3):437\u201349. https:\/\/doi.org\/10.1016\/j.str.2005.11.014.","journal-title":"Structure"},{"issue":"3","key":"4187_CR38","doi-asserted-by":"publisher","first-page":"e0119264","DOI":"10.1371\/JOURNAL.PONE.0119264","volume":"10","author":"L Mart\u00ednez","year":"2015","unstructured":"Mart\u00ednez L. Automatic identification of mobile and rigid substructures in molecular dynamics simulations and fractional structural fluctuation analysis. PLoS ONE. 2015;10(3):e0119264. https:\/\/doi.org\/10.1371\/JOURNAL.PONE.0119264.","journal-title":"PLoS ONE"},{"issue":"1","key":"4187_CR39","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1002\/BTPR.710","volume":"28","author":"D Shah","year":"2012","unstructured":"Shah D, Li J, Shaikh AR, Rajagopalan R. Arginine-aromatic interactions and their effects on arginine-induced solubilization of aromatic solutes and suppression of protein aggregation. Biotechnol Prog. 2012;28(1):223\u201331. https:\/\/doi.org\/10.1002\/BTPR.710.","journal-title":"Biotechnol Prog"},{"issue":"41","key":"4187_CR40","doi-asserted-by":"publisher","first-page":"17289","DOI":"10.1039\/C5NR04274G","volume":"7","author":"M Hernando-P\u00e9rez","year":"2015","unstructured":"Hernando-P\u00e9rez M, Cartagena-Rivera AX, Lo\u0161dorfer Bo\u017ei\u010d A, Carrillo PJP, San Mart\u00edn C, Mateu MG, et al. Quantitative nanoscale electrostatics of viruses. Nanoscale. 2015;7(41):17289\u201398. https:\/\/doi.org\/10.1039\/C5NR04274G.","journal-title":"Nanoscale"},{"issue":"5","key":"4187_CR41","doi-asserted-by":"publisher","first-page":"1346","DOI":"10.1016\/j.xphs.2022.01.002","volume":"111","author":"JS Bee","year":"2022","unstructured":"Bee JS, Zhang Y (Zoe), Phillippi MK, Finkner S, Mezghebe T, Webber K, et al. Impact of time out of intended storage and freeze-thaw rates on the stability of adeno-associated virus 8 and 9. J Pharm Sci. 2022;111(5):1346\u201353. https:\/\/doi.org\/10.1016\/j.xphs.2022.01.002.","journal-title":"J Pharm Sci"},{"issue":"1","key":"4187_CR42","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.ymthe.2005.02.021","volume":"12","author":"JF Wright","year":"2005","unstructured":"Wright JF, Le T, Prado J, Bahr-Davidson J, Smith PH, Zhen Z, et al. Identification of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent its occurrence during vector purification and formulation. Mol Ther. 2005;12(1):171\u20138. https:\/\/doi.org\/10.1016\/j.ymthe.2005.02.021.","journal-title":"Mol Ther"},{"issue":"13","key":"4187_CR43","doi-asserted-by":"publisher","first-page":"1160","DOI":"10.1016\/j.addr.2011.06.015","volume":"63","author":"HJ Lee","year":"2011","unstructured":"Lee HJ, McAuley A, Schilke KF, McGuire J. Molecular origins of surfactant-mediated stabilization of protein drugs. Adv Drug Deliv Rev. 2011;63(13):1160\u201371. https:\/\/doi.org\/10.1016\/j.addr.2011.06.015.","journal-title":"Adv Drug Deliv Rev"},{"issue":"5","key":"4187_CR44","doi-asserted-by":"publisher","first-page":"1337","DOI":"10.1002\/JPS.23908","volume":"103","author":"HL Kim","year":"2014","unstructured":"Kim HL, Mcauley A, Mcguire J. Protein effects on surfactant adsorption suggest the dominant mode of surfactant-mediated stabilization of protein. J Pharm Sci. 2014;103(5):1337\u201345. https:\/\/doi.org\/10.1002\/JPS.23908.","journal-title":"J Pharm Sci"},{"issue":"1","key":"4187_CR45","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1021\/ACSBIOMATERIALS.3C00636","volume":"10","author":"J Kehrein","year":"2024","unstructured":"Kehrein J, Sotriffer C. Molecular dynamics simulations for rationalizing polymer bioconjugation strategies: challenges, recent developments, and future opportunities. ACS Biomater Sci Eng. 2024;10(1):51\u201374. https:\/\/doi.org\/10.1021\/ACSBIOMATERIALS.3C00636.","journal-title":"ACS Biomater Sci Eng"}],"container-title":["Pharmaceutical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11095-026-04187-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11095-026-04187-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11095-026-04187-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T18:46:40Z","timestamp":1790102800000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11095-026-04187-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,22]]},"references-count":45,"alternative-id":["4187"],"URL":"https:\/\/doi.org\/10.1007\/s11095-026-04187-0","relation":{},"ISSN":["0724-8741","1573-904X"],"issn-type":[{"value":"0724-8741","type":"print"},{"value":"1573-904X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,22]]},"assertion":[{"value":"4 June 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 August 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 September 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}]}}