{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:43:59Z","timestamp":1768524239072,"version":"3.49.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T00:00:00Z","timestamp":1620172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T00:00:00Z","timestamp":1620172800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["427912\/2018-0"],"award-info":[{"award-number":["427912\/2018-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["25191"],"award-info":[{"award-number":["25191"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Pituitary"],"published-print":{"date-parts":[[2021,10]]},"DOI":"10.1007\/s11102-021-01151-6","type":"journal-article","created":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T19:03:24Z","timestamp":1620241404000},"page":"746-753","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Identification of mutant K-RAS in pituitary macroadenoma"],"prefix":"10.1007","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2751-0986","authenticated-orcid":false,"given":"Veronica","family":"Aran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manoela","family":"Heringer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Jose","family":"da Mata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leandro","family":"Kasuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renan Lyra","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe","family":"Andreiuolo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leila","family":"Chimelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Niemeyer","family":"Filho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica Roberto","family":"Gadelha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivaldo Moura","family":"Neto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,5,5]]},"reference":[{"key":"1151_CR1","doi-asserted-by":"publisher","DOI":"10.4251\/wjgo.v5.i5.97","author":"K Zenonos","year":"2013","unstructured":"Zenonos K (2013) RAS signaling pathways, mutations and their role in colorectal cancer. World J Gastrointest Oncol. https:\/\/doi.org\/10.4251\/wjgo.v5.i5.97","journal-title":"World J Gastrointest Oncol"},{"key":"1151_CR2","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1038\/s41568-018-0076-6","volume":"18","author":"S Li","year":"2018","unstructured":"Li S, Balmain A, Counter CM (2018) A model for RAS mutation patterns in cancers: finding the sweet spot. Nat Rev Cancer 18:767\u2013777","journal-title":"Nat Rev Cancer"},{"key":"1151_CR3","doi-asserted-by":"publisher","first-page":"1287","DOI":"10.1242\/jcs.182873","volume":"129","author":"GA Hobbs","year":"2016","unstructured":"Hobbs GA, Der CJ, Rossman KL (2016) RAS isoforms and mutations in cancer at a glance. J Cell Sci 129:1287\u20131292. https:\/\/doi.org\/10.1242\/jcs.182873","journal-title":"J Cell Sci"},{"key":"1151_CR4","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1186\/1471-230X-14-73","volume":"14","author":"C Gil Ferreira","year":"2014","unstructured":"Gil Ferreira C, Aran V, Zalcberg-Renault I et al (2014) KRAS mutations: variable incidences in a Brazilian cohort of 8,234 metastatic colorectal cancer patients. BMC Gastroenterol 14:73. https:\/\/doi.org\/10.1186\/1471-230X-14-73","journal-title":"BMC Gastroenterol"},{"key":"1151_CR5","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/J.LUNGCAN.2017.12.005","volume":"116","author":"V Aran","year":"2018","unstructured":"Aran V, Masson Domingues P, Carvalho de Macedo F et al (2018) A cross-sectional study examining the expression of splice variants K-RAS4A and K-RAS4B in advanced non-small-cell lung cancer patients. Lung Cancer 116:7\u201314. https:\/\/doi.org\/10.1016\/J.LUNGCAN.2017.12.005","journal-title":"Lung Cancer"},{"key":"1151_CR6","doi-asserted-by":"publisher","first-page":"2969","DOI":"10.1158\/0008-5472.CAN-19-3682","volume":"80","author":"IA Prior","year":"2020","unstructured":"Prior IA, Hood FE, Hartley JL (2020) The frequency of Ras mutations in cancer. Cancer Res 80:2969\u20132974","journal-title":"Cancer Res"},{"key":"1151_CR7","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djw036","author":"S Kato","year":"2016","unstructured":"Kato S, Lippman SM, Flaherty KT, Kurzrock R (2016) The conundrum of genetic \u201cDrivers\u201d in benign conditions. J Natl Cancer Inst. https:\/\/doi.org\/10.1093\/jnci\/djw036","journal-title":"J Natl Cancer Inst"},{"key":"1151_CR8","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-018-0194-4","author":"BP Coura","year":"2019","unstructured":"Coura BP, Bernardes VF, de Sousa SF et al (2019) KRAS mutations drive adenomatoid odontogenic tumor and are independent of clinicopathological features. Mod Pathol. https:\/\/doi.org\/10.1038\/s41379-018-0194-4","journal-title":"Mod Pathol"},{"key":"1151_CR9","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.05.007","author":"Y Feng","year":"2011","unstructured":"Feng Y, Bommer GT, Zhao J et al (2011) Mutant kras promotes hyperplasia and alters differentiation in the colon epithelium but does not expand the presumptive stem cell pool. Gastroenterology. https:\/\/doi.org\/10.1053\/j.gastro.2011.05.007","journal-title":"Gastroenterology"},{"key":"1151_CR10","doi-asserted-by":"publisher","DOI":"10.1002\/cjp2.66","author":"M Wittersheim","year":"2017","unstructured":"Wittersheim M, Heydt C, Hoffmann F, B\u00fcttner R (2017) KRAS mutation in papillary fibroelastoma: a true cardiac neoplasm? J Pathol Clin Res. https:\/\/doi.org\/10.1002\/cjp2.66","journal-title":"J Pathol Clin Res"},{"issue":"20","key":"1151_CR11","doi-asserted-by":"publisher","first-page":"5030","DOI":"10.1158\/1078-0432.CCR-16-0452","volume":"22","author":"F Caimari","year":"2016","unstructured":"Caimari F, Korbonits M (2016) Novel genetic causes of pituitary adenomas. Clin Cancer Res 22(20):5030\u20135042","journal-title":"Clin Cancer Res"},{"issue":"12","key":"1151_CR12","doi-asserted-by":"publisher","first-page":"4769","DOI":"10.1210\/jc.2006-1668","volume":"91","author":"AF Daly","year":"2006","unstructured":"Daly AF, Rixhon M, Adam C et al (2006) High prevalence of pituitary adenomas: A cross-sectional study in the province of Li\u00e8ge Belgium. J Clin Endocrinol Metab 91(12):4769\u20134775. https:\/\/doi.org\/10.1210\/jc.2006-1668","journal-title":"J Clin Endocrinol Metab"},{"key":"1151_CR13","doi-asserted-by":"publisher","first-page":"v1","DOI":"10.1093\/neuonc\/noz150","volume":"21","author":"QT Ostrom","year":"2019","unstructured":"Ostrom QT, Cioffi G, Gittleman H et al (2019) CBTRUS statistical report: primary brain and other central nervous system tumors diagnosed in the United States in 2012\u20132016. Neuro Oncol 21:v1\u2013v100. https:\/\/doi.org\/10.1093\/neuonc\/noz150","journal-title":"Neuro Oncol"},{"key":"1151_CR14","doi-asserted-by":"publisher","first-page":"256","DOI":"10.5892\/ruvrd.v13i1.1979","volume":"13","author":"CC Carneiro","year":"2015","unstructured":"Carneiro CC, Mendes BB, Bastos LG (2015) Adenoma hipofis\u00e1rio: correla\u00e7\u00e3o cl\u00ednica, laboratorial e radiol\u00f3giCA. Rev da Univ Val do Rio Verde 13:256\u2013269. https:\/\/doi.org\/10.5892\/ruvrd.v13i1.1979","journal-title":"Rev da Univ Val do Rio Verde"},{"key":"1151_CR15","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-14-0305","author":"I Potorac","year":"2015","unstructured":"Potorac I, Petrossians P, Daly AF et al (2015) Pituitary MRI characteristics in 297 acromegaly patients based on T2-weighted sequences. Endocr Relat Cancer. https:\/\/doi.org\/10.1530\/ERC-14-0305","journal-title":"Endocr Relat Cancer"},{"key":"1151_CR16","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1007\/s11154-019-09531-x","volume":"21","author":"L Kasuki","year":"2020","unstructured":"Kasuki L, Raverot G (2020) Definition and diagnosis of aggressive pituitary tumors. Rev Endocr Metab Disord 21:203\u2013208","journal-title":"Rev Endocr Metab Disord"},{"key":"1151_CR17","doi-asserted-by":"publisher","first-page":"237","DOI":"10.3803\/EnM.2020.35.2.237","volume":"35","author":"SO Chin","year":"2020","unstructured":"Chin SO (2020) Epidemiology of functioning pituitary adenomas. Endocrinol Metab 35:237\u2013242. https:\/\/doi.org\/10.3803\/EnM.2020.35.2.237","journal-title":"Endocrinol Metab"},{"key":"1151_CR18","doi-asserted-by":"publisher","first-page":"30","DOI":"10.3390\/jcm9010030","volume":"9","author":"C Tatsi","year":"2019","unstructured":"Tatsi C, Stratakis CA (2019) The genetics of pituitary adenomas. J Clin Med 9:30. https:\/\/doi.org\/10.3390\/jcm9010030","journal-title":"J Clin Med"},{"key":"1151_CR19","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.ghir.2016.09.007","volume":"30\u201331","author":"D Iacovazzo","year":"2016","unstructured":"Iacovazzo D, Korbonits M (2016) Gigantism: x-linked acrogigantism and GPR101 mutations. Growth Horm IGF Res 30\u201331:64\u201369","journal-title":"Growth Horm IGF Res"},{"key":"1151_CR20","doi-asserted-by":"publisher","first-page":"842","DOI":"10.1210\/jcem.78.4.8157709","volume":"78","author":"L Pei","year":"1994","unstructured":"Pei L, Melmed S, Scheithauer B et al (1994) H-ras mutations in human pituitary carcinoma metastases. J Clin Endocrinol Metab 78:842\u2013846. https:\/\/doi.org\/10.1210\/jcem.78.4.8157709","journal-title":"J Clin Endocrinol Metab"},{"key":"1151_CR21","doi-asserted-by":"publisher","first-page":"914","DOI":"10.1210\/jcem.74.4.1312542","volume":"74","author":"HJ Karga","year":"1992","unstructured":"Karga HJ, Alexander JM, Hedley-Whyte ET et al (1992) Ras mutations in human pituitary tumors. J Clin Endocrinol Metab 74:914\u2013919. https:\/\/doi.org\/10.1210\/jcem.74.4.1312542","journal-title":"J Clin Endocrinol Metab"},{"key":"1151_CR22","doi-asserted-by":"publisher","first-page":"a031435","DOI":"10.1101\/cshperspect.a031435","volume":"8","author":"AM Waters","year":"2018","unstructured":"Waters AM, Der CJ (2018) KRAS: the critical driver and therapeutic target for pancreatic cancer. Cold Spring Harb Perspect Med 8:a031435. https:\/\/doi.org\/10.1101\/cshperspect.a031435","journal-title":"Cold Spring Harb Perspect Med"},{"issue":"3","key":"1151_CR23","first-page":"36","volume":"3","author":"N Karachaliou","year":"2015","unstructured":"Karachaliou N, Mayo-de-las-Casas C, Molina-Vila MA, Rosell R (2015) Real-time liquid biopsies become a reality in cancer treatment. Ann Transl Med 3(3):36","journal-title":"Ann Transl Med"},{"issue":"6","key":"1151_CR24","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1158\/2159-8290.CD-13-1014","volume":"4","author":"DA Haber","year":"2014","unstructured":"Haber DA, Velculescu VE (2014) Blood-based analyses of cancer: circulating tumor cells and circulating tumor DNA. Cancer Discov. 4(6):650\u2013661","journal-title":"Cancer Discov."},{"key":"1151_CR25","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3233","author":"LA Diaz","year":"2013","unstructured":"Diaz LA, Polyak K (2013) Tracking tumor resistance using \u2018liquid biopsies.\u2019 Nat Med. https:\/\/doi.org\/10.1038\/nm.3233","journal-title":"Nat Med"},{"key":"1151_CR26","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aay1984","author":"AK Mattox","year":"2019","unstructured":"Mattox AK, Bettegowda C, Zhou S et al (2019) Applications of liquid biopsies for cancer. Sci Transl Med. https:\/\/doi.org\/10.1126\/scitranslmed.aay1984","journal-title":"Sci Transl Med"},{"issue":"5","key":"1151_CR27","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1007\/s40291-017-0278-8","volume":"21","author":"S Olmedillas-L\u00f3pez","year":"2017","unstructured":"Olmedillas-L\u00f3pez S, Garc\u00eda-Arranz M, Garc\u00eda-Olmo D (2017) Current and emerging applications of droplet digital PCR in oncology. Mol Diagnosis Ther 21(5):493\u2013510","journal-title":"Mol Diagnosis Ther"},{"key":"1151_CR28","doi-asserted-by":"publisher","first-page":"9650","DOI":"10.1038\/s41598-018-27368-3","volume":"8","author":"L Dong","year":"2018","unstructured":"Dong L, Wang S, Fu B, Wang J (2018) Evaluation of droplet digital PCR and next generation sequencing for characterizing DNA reference material for KRAS mutation detection. Sci Reports 8:9650. https:\/\/doi.org\/10.1038\/s41598-018-27368-3","journal-title":"Sci Reports"},{"key":"1151_CR29","doi-asserted-by":"publisher","first-page":"e0139074","DOI":"10.1371\/journal.pone.0139074","volume":"10","author":"A Pender","year":"2015","unstructured":"Pender A, Garcia-Murillas I, Rana S et al (2015) Efficient genotyping of KRAS mutant non-small cell lung cancer using a multiplexed droplet digital PCR approach. PLoS ONE 10:e0139074. https:\/\/doi.org\/10.1371\/journal.pone.0139074","journal-title":"PLoS ONE"},{"key":"1151_CR30","doi-asserted-by":"publisher","first-page":"1221","DOI":"10.1016\/j.molonc.2016.05.009","volume":"10","author":"JA Denis","year":"2016","unstructured":"Denis JA, Patroni A, Guillerm E et al (2016) Droplet digital PCR of circulating tumor cells from colorectal cancer patients can predict KRAS mutations before surgery. Mol Oncol 10:1221\u20131231. https:\/\/doi.org\/10.1016\/j.molonc.2016.05.009","journal-title":"Mol Oncol"},{"issue":"3","key":"1151_CR31","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/j.tips.2019.01.006","volume":"40","author":"G De Rubis","year":"2019","unstructured":"De Rubis G, Rajeev Krishnan S, Bebawy M (2019) Liquid biopsies in cancer diagnosis, monitoring, and prognosis. Trends Pharmacol Sci 40(3):172\u2013186","journal-title":"Trends Pharmacol Sci"},{"issue":"6","key":"1151_CR32","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1200\/JCO.2012.45.2011","volume":"32","author":"LA Diaz","year":"2014","unstructured":"Diaz LA, Bardelli A (2014) Liquid biopsies: genotyping circulating tumor DNA. J Clin Oncol 32(6):579\u2013586","journal-title":"J Clin Oncol"},{"key":"1151_CR33","doi-asserted-by":"publisher","first-page":"83","DOI":"10.2217\/pme.14.69","volume":"12","author":"MB Myers","year":"2015","unstructured":"Myers MB, Mckim KL, Meng F, Parsons BL (2015) Low-frequency KRAS mutations are prevalent in lung adenocarcinomas. Per Med 12:83\u201398. https:\/\/doi.org\/10.2217\/pme.14.69","journal-title":"Per Med"},{"key":"1151_CR34","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.1093\/annonc\/mds620","volume":"24","author":"D Tougeron","year":"2013","unstructured":"Tougeron D, Lecomte T, Pag\u00e8s JC et al (2013) Effect of low-frequency KRAS mutations on the response to anti-EGFR therapy in metastatic colorectal cancer. Ann Oncol 24:1267\u20131273. https:\/\/doi.org\/10.1093\/annonc\/mds620","journal-title":"Ann Oncol"}],"container-title":["Pituitary"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11102-021-01151-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11102-021-01151-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11102-021-01151-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,3]],"date-time":"2021-09-03T23:53:35Z","timestamp":1630713215000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11102-021-01151-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,5]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["1151"],"URL":"https:\/\/doi.org\/10.1007\/s11102-021-01151-6","relation":{},"ISSN":["1386-341X","1573-7403"],"issn-type":[{"value":"1386-341X","type":"print"},{"value":"1573-7403","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,5]]},"assertion":[{"value":"28 April 2021","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 May 2021","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors have no relevant financial or non-financial interests to disclose.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}},{"value":"This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of <i>Instituto Estadual do C\u00e9rebro Paulo Niemeyer<\/i> (Approval number: CAAE:90680218.6.1001.8110).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}}]}}