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PEG is shown to increase the critical micelle concentration (CMC) of all three detergents in the crystallization buffer in a way that the free energy of micelle formation increases linearly with the concentration of oxyethylene units (O\u2013CH\n                    <jats:sub>2<\/jats:sub>\n                    \u2013CH\n                    <jats:sub>2<\/jats:sub>\n                    ) irrespective of the actual molecular weight of the polymer. The CMC shift is modeled by assuming for simplicity that it is dominated by the interaction between PEG and detergent monomers and is interpreted in terms of an increase of the transfer free energy of a methylene group of the alkyl chain by 0.2\u00a0kJ\u00a0mol\n                    <jats:sup>\u22121<\/jats:sup>\n                    per 1\u00a0mol L\n                    <jats:sup>\u22121<\/jats:sup>\n                    increase of the concentration of oxyethylene units at 298\u00a0K. Implications of this effect for the solubilization and crystallization of protein\u2013detergent complexes as well as detergent extraction from crystals are discussed.\n                  <\/jats:p>","DOI":"10.1007\/s11120-024-01079-5","type":"journal-article","created":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T12:01:51Z","timestamp":1710504111000},"page":"273-289","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Towards understanding the crystallization of photosystem II: influence of poly(ethylene glycol) of various molecular sizes on the micelle formation of alkyl maltosides"],"prefix":"10.1007","volume":"162","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8818-2616","authenticated-orcid":false,"given":"Frank","family":"M\u00fch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2710-1273","authenticated-orcid":false,"given":"Adrian","family":"Bothe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0561-6990","authenticated-orcid":false,"given":"Athina","family":"Zouni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,3,15]]},"reference":[{"key":"1079_CR1","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1006\/jcis.1996.4648","volume":"186","author":"EB Abuin","year":"1997","unstructured":"Abuin EB, Lissi EA, Asp\u00e9e A, Gonzalez FD, Varas JM (1997) Fluorescence of 8-anilinonaphthalene-1-sulfonate and properties of sodium dodecyl sulfate micelles in water-urea mixtures. 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