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To understand the role of various possible conformations of the CaMn<jats:sub>4<\/jats:sub>O<jats:sub>5<\/jats:sub>-cluster in this process, the temperature dependence of the S<jats:sub>1<\/jats:sub>\u2009\u2192\u2009S<jats:sub>2<\/jats:sub> and S<jats:sub>2<\/jats:sub>\u2009\u2192\u2009S<jats:sub>3<\/jats:sub> state transitions, induced by saturating laser flashes, was studied in spinach photosystem II membrane preparations under different conditions. The S<jats:sub>1<\/jats:sub>\u2009\u2192\u2009S<jats:sub>2<\/jats:sub> transition temperature dependence was shown to be much dependent on the type of the cryoprotectant and presence of 3.5% methanol, resulting in the variation of transition half-inhibition temperature by 50 K. No similar effect was observed for the S<jats:sub>2<\/jats:sub>\u2009\u2192\u2009S<jats:sub>3<\/jats:sub> state transition, for which we also show that both the low spin g\u2009=\u20092.0 multiline and high spin g\u2009=\u20094.1 EPR configurations of the S<jats:sub>2<\/jats:sub> state advance with similar efficiency to the S<jats:sub>3<\/jats:sub> state, both showing a transition half-inhibition temperature of 240 K. This was further confirmed by following the appearance of the Split S<jats:sub>3<\/jats:sub> EPR signal. The results are discussed in relevance to the functional and structural heterogeneity of the water oxidizing complex intermediates in photosystem II.<\/jats:p>","DOI":"10.1007\/s11120-024-01096-4","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T14:02:54Z","timestamp":1714053774000},"page":"401-411","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The S1 to S2 and S2 to S3 state transitions in plant photosystem II: relevance to the functional and structural heterogeneity of the water oxidizing complex"],"prefix":"10.1007","volume":"162","author":[{"given":"Andrea","family":"Pavlou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stenbj\u00f6rn","family":"Styring","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fikret","family":"Mamedov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,25]]},"reference":[{"issue":"43","key":"1096_CR1","doi-asserted-by":"publisher","first-page":"13148","DOI":"10.1021\/bi971815w","volume":"36","author":"KA \u00c5hrling","year":"1997","unstructured":"\u00c5hrling KA, Peterson S, Styring S (1997) An oscillating manganese electron paramagnetic resonance signal from the S0 state of the oxygen evolving complex in photosystem II. 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