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The speed at which leaf metabolism transitions between these pathways likely influences plant tolerance to fluctuating light conditions. To investigate these rapid metabolic shifts, we exposed leaves to \u00b9\u00b3CO\u2082 for 20\u00a0min to establish a quasi-steady state before abruptly turning off the light while maintaining \u00b9\u00b3CO\u2082 feeding. Within 10\u00a0s of dark transition, 3-phosphoglycerate levels rose significantly while most other CBB cycle intermediates decreased by more than 90%. Simultaneously, carbon accumulated in alanine, likely via pyruvate. Over the subsequent 10\u00a0min, six- and five-carbon TCA cycle intermediates steadily increased. In contrast, four-carbon TCA intermediates peaked at one minute, declined at three minutes, and rose again at 10\u00a0min, a pattern mirrored by most measured amino acids. These results reveal an exceptionally rapid metabolic reconfiguration from CO\u2082 fixation by the CBB cycle in light to TCA cycle activation for energy production in darkness, accompanied by substantial changes in amino acid metabolism.<\/jats:p>","DOI":"10.1007\/s11120-025-01173-2","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T17:26:05Z","timestamp":1759166765000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Time-resolved targeted metabolomics shows an abrupt switch from Calvin-Benson-Bassham cycle to tricarboxylic acid cycle when the light is turned off"],"prefix":"10.1007","volume":"163","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8259-2315","authenticated-orcid":false,"given":"Yuan","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9054-5538","authenticated-orcid":false,"given":"Stephanie C.","family":"Schmiege","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4423-3223","authenticated-orcid":false,"given":"Thomas D.","family":"Sharkey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,9,29]]},"reference":[{"issue":"4","key":"1173_CR2","doi-asserted-by":"publisher","first-page":"1079","DOI":"10.1111\/nph.14719","volume":"216","author":"C Abadie","year":"2017","unstructured":"Abadie C, Lothier J, Boex-Fontvieille E, Carroll A, Tcherkez G (2017) Direct assessment of the metabolic origin of carbon atoms in glutamate from illuminated leaves using 13C-NMR. 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