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Phys Chem Chem Phys 2:1301\u20131304, 2000), it is shown that the specificity constant\n                    <jats:inline-formula>\n                      <jats:tex-math>$$\\:{\\Gamma\\:}$$<\/jats:tex-math>\n                    <\/jats:inline-formula>\n                    , indicating the ability of RUBISCO to discriminate between CO\n                    <jats:sub>2<\/jats:sub>\n                    and O\n                    <jats:sub>2<\/jats:sub>\n                    as substrate, can be determined from an isothermal titration calorimetric (ITC) measurement of\n                    <jats:inline-formula>\n                      <jats:tex-math>$$\\:{\\varDelta\\:}_{\\text{r}}H$$<\/jats:tex-math>\n                    <\/jats:inline-formula>\n                    as a function of the substrate concentration ratio\n                    <jats:inline-formula>\n                      <jats:tex-math>$$\\:\\rho\\:=\\left[\\text{C}{\\text{O}}_{2}\\right]\/\\left[{\\text{O}}_{2}\\right]$$<\/jats:tex-math>\n                    <\/jats:inline-formula>\n                    . The approach does not need any radioactive materials and might be a cost-effective alternative for RUBISCO engineering to screen for variants with a high specificity constant.\n                  <\/jats:p>","DOI":"10.1007\/s11120-025-01187-w","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T13:47:38Z","timestamp":1764596858000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Calculation of specificity constants \u0393 for RUBISCO of different species from microcalorimetric data"],"prefix":"10.1007","volume":"163","author":[{"given":"Joachim","family":"Frank","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8818-2616","authenticated-orcid":false,"given":"Frank","family":"M\u00fch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,1]]},"reference":[{"key":"1187_CR1","doi-asserted-by":"publisher","unstructured":"Aguil\u00f3-Nicolau P, Galmes J, Fais G, Capo-Bauca S, Cao GCM, Iniguez C (2023) Singular adaptations in the carbon assimilation mechanism of the polyextremophile Cyanobacterium Chroococcidiopsis thermalis. 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