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(<jats:italic>U. dioica<\/jats:italic>) stands out as a potential therapeutic candidate with its anti-oxidant, anti-cancer and lipid-lowering properties. In this study, the molecular effects of <jats:italic>U. dioica<\/jats:italic> were investigated by gene expression analysis and molecular docking methods. <jats:italic>U. dioica<\/jats:italic> significantly suppressed the expression of Brca1, Brca2, Fas, Lpl, Dgat1 and Mcp1 genes, resulting in significant changes in lipid metabolism, cancer susceptibility and inflammation. Molecular docking analyses showed that <jats:italic>U. dioica<\/jats:italic> components have strong binding affinities with target proteins. In particular, the interactions between Dgat1-Isorhamnetin rutinoside (-10.3\u00a0kcal\/mol), Fas-Quercetin acetyl rutinoside (-10.3\u00a0kcal\/mol), Lpl-Apigenin hexoside (-9.2\u00a0kcal\/mol) and Mcp1-Quercetin acetyl rutinoside (-8.6\u00a0kcal\/mol) were notable. In vitro and in silico analyses supported each other, revealing the effects of <jats:italic>U. dioica<\/jats:italic> in gene expression regulation and the potential for its constituents to interact with proteins. These findings indicate that <jats:italic>U. dioica<\/jats:italic> may be a promising alternative therapeutic agent in the treatment of obesity and obesity-related breast cancer and emphasize that its efficacy should be confirmed by clinical trials.<\/jats:p>","DOI":"10.1007\/s11130-025-01341-8","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T05:01:08Z","timestamp":1743397268000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exploring Urtica dioica L. as a Promising Alternative Therapy for Obesity-Related Breast Cancer: Insights from Molecular Mechanisms and Bioinformatic Analysis"],"prefix":"10.1007","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5521-6402","authenticated-orcid":false,"given":"Ayla","family":"Eren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2565-453X","authenticated-orcid":false,"given":"Mehmet","family":"Varol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1365-2310","authenticated-orcid":false,"given":"Resat","family":"Unal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6358-2448","authenticated-orcid":false,"given":"Filiz","family":"Altan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,28]]},"reference":[{"key":"1341_CR1","first-page":"35","volume":"2","author":"M Urhan","year":"2017","unstructured":"Urhan M, Akbulut G, Ruh M et al (2017) Obesity and cancer relationship: Is leptin a carcinogenic adipokine? \u0130zmir Katip \u00c7elebi Univ Faculty Health Sci J 2:35\u201343","journal-title":"\u0130zmir Katip \u00c7elebi Univ Faculty Health Sci J"},{"key":"1341_CR2","doi-asserted-by":"publisher","first-page":"62","DOI":"10.3390\/CANCERS11010062","volume":"11","author":"S And\u00f2","year":"2019","unstructured":"And\u00f2 S, Gelsomino L, Panza S et al (2019) Obesity, Leptin and Breast Cancer: epidemiological evidence and proposed mechanisms. 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