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Whilst transgenic mice have been generated\u00a0for many Hippo genes, the resultant skeletal muscle phenotypes were not always characterized. Here, we aimed to phenotype the hindlimb muscles of Hippo gene-mutated <jats:italic>Lats1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>, <jats:italic>Mst2<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>, <jats:italic>Vgll3<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>, and <jats:italic>Vgll4<\/jats:italic><jats:sup><jats:italic>+\/\u2212<\/jats:italic><\/jats:sup> mice. This analysis revealed that <jats:italic>Lats1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice have 11% more slow type I fibers than age and sex-matched wild-type controls. Moreover, the mRNA expression of slow <jats:italic>Myh7<\/jats:italic> increased by 50%, and the concentration of type I myosin heavy chain is 80% higher in <jats:italic>Lats1<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice than in age and sex-matched wild-type controls. Second, to find out whether exercise-related stimuli affect Lats1, we stimulated C2C12 myotubes with the hypertrophy agent clenbuterol or the energy stress agent AICAR. We found that both stimulated <jats:italic>Lats1<\/jats:italic> expression by 1.2 and 1.3 fold respectively. Third, we re-analyzed published datasets and found that <jats:italic>Lats1<\/jats:italic> mRNA in muscle is 63% higher in muscular dystrophy, increases by 17\u201377% after cardiotoxin-induced muscle injury, by 41\u201371% in muscles during overload-induced hypertrophy, and by 19\u201321% after endurance exercise when compared to respective controls. To conclude, <jats:italic>Lats1<\/jats:italic> contributes to the regulation of muscle fiber type proportions, and its expression is regulated by physiological and pathological situations in skeletal muscle.<\/jats:p>","DOI":"10.1007\/s11248-021-00293-4","type":"journal-article","created":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T01:02:31Z","timestamp":1641344551000},"page":"227-237","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Skeletal muscle phenotyping of Hippo gene-mutated mice reveals that Lats1 deletion increases the percentage of type I muscle fibers"],"prefix":"10.1007","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8518-8409","authenticated-orcid":false,"given":"Fakhreddin Yaghoob","family":"Nezhad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annett","family":"Riermeier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Sch\u00f6nfelder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6890-4984","authenticated-orcid":false,"given":"Lore","family":"Becker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7898-2353","authenticated-orcid":false,"given":"Martin Hrab\u0115","family":"de Angelis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5920-5842","authenticated-orcid":false,"given":"Henning","family":"Wackerhage","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,1,5]]},"reference":[{"issue":"1","key":"293_CR1","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1242\/dmm.010389","volume":"6","author":"P Bonaldo","year":"2013","unstructured":"Bonaldo P, Sandri M (2013) Cellular and molecular mechanisms of muscle atrophy. 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