{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:16:38Z","timestamp":1789535798500,"version":"build-2803163510"},"reference-count":63,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Transgenic Res"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11248-025-00433-0","type":"journal-article","created":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T18:52:09Z","timestamp":1743274329000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Plant-derived recombinant macromolecular PAP-IgG Fc as a novel prostate cancer vaccine candidate eliciting robust immune responses"],"prefix":"10.1007","volume":"34","author":[{"given":"Yangjoo","family":"Kang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deuk-Su","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyunjoo","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yerin","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Jin","family":"Seo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Hinterdorfer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kisung","family":"Ko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"issue":"7","key":"433_CR1","doi-asserted-by":"publisher","first-page":"4826","DOI":"10.1074\/jbc.M109.081828","volume":"285","author":"JT Andersen","year":"2010","unstructured":"Andersen JT, Daba MB, Berntzen G, Michaelsen TE, Sandlie I (2010) Cross-species binding analyses of mouse and human neonatal Fc receptor show dramatic differences in immunoglobulin G and albumin binding. J Biol Chem 285(7):4826\u20134836. https:\/\/doi.org\/10.1074\/jbc.M109.081828","journal-title":"J Biol Chem"},{"key":"433_CR2","doi-asserted-by":"publisher","first-page":"1149455","DOI":"10.3389\/fpls.2023.1149455","volume":"14","author":"CJI Bulaon","year":"2023","unstructured":"Bulaon CJI, Khorattanakulchai N, Rattanapisit K, Sun H, Pisuttinusart N, Strasser R, Tanaka S, Soon-Shiong P, Phoolcharoen W (2023) Antitumor effect of plant-produced anti-CTLA-4 monoclonal antibody in a murine model of colon cancer. Front Plant Sci 14:1149455. https:\/\/doi.org\/10.3389\/fpls.2023.1149455","journal-title":"Front Plant Sci"},{"issue":"2b","key":"433_CR3","doi-asserted-by":"publisher","first-page":"E99","DOI":"10.1111\/j.1464-410X.2011.10790.x","volume":"110","author":"G Di Lorenzo","year":"2012","unstructured":"Di Lorenzo G, Ferro M, Buonerba C (2012) Sipuleucel-T (Provenge\u00ae) for castration-resistant prostate cancer. Bju Int 110(2b):E99\u2013E104. https:\/\/doi.org\/10.1111\/j.1464-410X.2011.10790.x","journal-title":"Bju Int"},{"issue":"7","key":"433_CR4","doi-asserted-by":"publisher","first-page":"3113","DOI":"10.4049\/jimmunol.159.7.3113","volume":"159","author":"L Fong","year":"1997","unstructured":"Fong L, Ruegg CL, Brockstedt D, Engleman EG, Laus R (1997) Induction of tissue-specific autoimmune prostatitis with prostatic acid phosphatase immunization\u2014implications for immunotherapy of prostate cancer. J Immunol 159(7):3113\u20133117","journal-title":"J Immunol"},{"issue":"12","key":"433_CR5","doi-asserted-by":"publisher","first-page":"7150","DOI":"10.4049\/jimmunol.167.12.7150","volume":"167","author":"L Fong","year":"2001","unstructured":"Fong L, Brockstedt D, Benike C, Breen JK, Strang G, Ruegg CL, Engleman EG (2001) Dendritic cell-based xenoantigen vaccination for prostate cancer immunotherapy. J Immunol 167(12):7150\u20137156. https:\/\/doi.org\/10.4049\/jimmunol.167.12.7150","journal-title":"J Immunol"},{"issue":"4","key":"433_CR6","doi-asserted-by":"publisher","first-page":"1585","DOI":"10.3892\/or.2015.3770","volume":"33","author":"K Fujio","year":"2015","unstructured":"Fujio K, Watanabe M, Ueki H, Li SA, Kinoshita R, Ochiai K, Futami J, Watanabe T, Nasu Y, Kumon H (2015) A vaccine strategy with multiple prostatic acid phosphatase-fused cytokines for prostate cancer treatment. Oncol Rep 33(4):1585\u20131592. https:\/\/doi.org\/10.3892\/or.2015.3770","journal-title":"Oncol Rep"},{"issue":"3","key":"433_CR7","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1038\/nrurol.2013.8","volume":"10","author":"SM Geary","year":"2013","unstructured":"Geary SM, Lemke CD, Lubaroff DM, Salem AK (2013) Proposed mechanisms of action for prostate cancer vaccines. Nat Rev Urol 10(3):149\u2013160. https:\/\/doi.org\/10.1038\/nrurol.2013.8","journal-title":"Nat Rev Urol"},{"issue":"11","key":"433_CR8","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1016\/j.tibtech.2005.09.003","volume":"23","author":"W Gomord","year":"2005","unstructured":"Gomord W, Chamberlain P, Jefferis R, Faye L (2005) Biopharmaceutical production in plants: problems, solutions and opportunities. Trends Biotechnol 23(11):559\u2013565. https:\/\/doi.org\/10.1016\/j.tibtech.2005.09.003","journal-title":"Trends Biotechnol"},{"issue":"10","key":"433_CR9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2123002119","volume":"119","author":"A Gupta","year":"2022","unstructured":"Gupta A, Smith P, Bournazos S, Ravetch JV (2022) A novel mouse strain optimized for chronic human antibody administration. Proc Natl Acad Sci USA 119(10):e2123002119. https:\/\/doi.org\/10.1073\/pnas.2123002119","journal-title":"Proc Natl Acad Sci USA"},{"issue":"6","key":"433_CR10","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1002\/ijc.11292","volume":"106","author":"E J\u00e4ger","year":"2003","unstructured":"J\u00e4ger E, J\u00e4ger D, Knuth A (2003) Antigen-specific immunotherapy and cancer vaccines. Int J Cancer 106(6):817\u2013820. https:\/\/doi.org\/10.1002\/ijc.11292","journal-title":"Int J Cancer"},{"issue":"2","key":"433_CR11","doi-asserted-by":"publisher","first-page":"71","DOI":"10.3322\/CA.2007.0010","volume":"58","author":"A Jemal","year":"2008","unstructured":"Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T, Thun MJ (2008) Cancer statistics. CA Cancer J Clin 58(2):71\u201396. https:\/\/doi.org\/10.3322\/CA.2007.0010","journal-title":"CA Cancer J Clin"},{"issue":"6","key":"433_CR12","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1007\/s11816-023-00882-1","volume":"17","author":"CQ Jin","year":"2023","unstructured":"Jin CQ, Lee CE, Hwang H, Kim Y, Hinterdorfer P, Myung SC, Park S, Kim MK, Hong ME, Ko K (2023) Specific binding of plant-expressed anti-PD-L1 monoclonal antibody to multiple myeloma cell line RPMI8226. Plant Biotechnol Rep 17(6):865\u2013874. https:\/\/doi.org\/10.1007\/s11816-023-00882-1","journal-title":"Plant Biotechnol Rep"},{"issue":"1","key":"433_CR13","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1007\/s11240-012-0124-y","volume":"110","author":"K Kamo","year":"2012","unstructured":"Kamo K, Aebig J, Guaragna MA, James C, Hsu HT, Jordan R (2012) plants transformed with single-chain variable fragment antibodies to. Plant Cell Tissue Org 110(1):13\u201321. https:\/\/doi.org\/10.1007\/s11240-012-0124-y","journal-title":"Plant Cell Tissue Org"},{"issue":"3","key":"433_CR14","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1007\/s13580-016-0045-5","volume":"57","author":"Y Kang","year":"2016","unstructured":"Kang Y, Shin YK, Park SW, Ko K (2016) Effect of nitrogen deficiency on recombinant protein production and dimerization and growth in transgenic plants. Hortic Environ Biote 57(3):299\u2013307. https:\/\/doi.org\/10.1007\/s13580-016-0045-5","journal-title":"Hortic Environ Biote"},{"key":"433_CR15","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.00274","author":"YJ Kang","year":"2017","unstructured":"Kang YJ, Kim DS, Myung SC, Ko K (2017) Expression of a human prostatic acid phosphatase (PAP)-IgM Fc fusion protein in plants using tissue subculture. Front Plant Sci. https:\/\/doi.org\/10.3389\/fpls.2017.00274","journal-title":"Front Plant Sci"},{"issue":"1","key":"433_CR16","doi-asserted-by":"publisher","first-page":"87","DOI":"10.2478\/ebtj-2023-0006","volume":"7","author":"Y Kang","year":"2023","unstructured":"Kang Y, Kim D-S, Kim K, Myung S-C, Oh YJ, Park S, Hinterdorfer P, Ko K (2023a) Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant. EuroBiotech J 7(1):87\u201395. https:\/\/doi.org\/10.2478\/ebtj-2023-0006","journal-title":"EuroBiotech J"},{"issue":"7","key":"433_CR17","doi-asserted-by":"publisher","first-page":"392","DOI":"10.5483\/BMBRep.2022-0207","volume":"56","author":"YJ Kang","year":"2023","unstructured":"Kang YJ, Kim DS, Kim S, Seo YJ, Ko K (2023b) Plant-derived PAP proteins fused to immunoglobulin A and M Fc domains induce anti-prostate cancer immune response in mice. Bmb Rep 56(7):392\u2013397","journal-title":"Bmb Rep"},{"key":"433_CR18","doi-asserted-by":"publisher","first-page":"3109","DOI":"10.3389\/fimmu.2018.03109","volume":"9","author":"AER Kartikasari","year":"2018","unstructured":"Kartikasari AER, Prakash MD, Cox M, Wilson K, Boer JC, Cauchi JA, Plebanski M (2018) Therapeutic cancer vaccines-T cell responses and epigenetic modulation. Front Immunol 9:3109. https:\/\/doi.org\/10.3389\/fimmu.2018.03109","journal-title":"Front Immunol"},{"issue":"5","key":"433_CR19","doi-asserted-by":"publisher","first-page":"767","DOI":"10.5114\/aoms.2012.31610","volume":"8","author":"P Kawalec","year":"2012","unstructured":"Kawalec P, Paszulewicz A, Holko P, Pilc A (2012) Sipuleucel-T immunotherapy for castration-resistant prostate cancer. A systematic review and meta-analysis. Arch Med Sci 8(5):767\u2013775. https:\/\/doi.org\/10.5114\/aoms.2012.31610","journal-title":"Arch Med Sci"},{"issue":"4","key":"433_CR20","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1080\/07352689609701943","volume":"15","author":"AR Kermode","year":"1996","unstructured":"Kermode AR (1996) Mechanisms of intracellular protein transport and targeting in plant cells. Crit Rev Plant Sci 15(4):285\u2013423. https:\/\/doi.org\/10.1080\/07352689609701943","journal-title":"Crit Rev Plant Sci"},{"issue":"2","key":"433_CR21","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1158\/2326-6066.Cir-13-0216","volume":"2","author":"HJ Kim","year":"2014","unstructured":"Kim HJ, Cantor H (2014) CD4 T-cell subsets and tumor immunity: the helpful and the not-so-helpful. Cancer Immunol Res 2(2):91\u201398. https:\/\/doi.org\/10.1158\/2326-6066.Cir-13-0216","journal-title":"Cancer Immunol Res"},{"key":"433_CR22","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1007\/s00299-024-03377-7","volume":"43","author":"Y Kim","year":"2024","unstructured":"Kim Y, Hwang H, Lim S, Lee D, Kim K, Kang E, Cho S, Oh Y, Hinterdorfer P, Lee HJ, Ko K (2024) Plant-derived EpCAM-Fc fusion proteins induce in vivo immune response to produce IgGs inhibiting invasion and migration of colorectal cancer cells. Plant Cell Rep 43:302. https:\/\/doi.org\/10.1007\/s00299-024-03377-7","journal-title":"Plant Cell Rep"},{"issue":"13","key":"433_CR23","doi-asserted-by":"publisher","first-page":"8013","DOI":"10.1073\/pnas.0832472100","volume":"100","author":"K Ko","year":"2003","unstructured":"Ko K, Tekoah Y, Rudd PM, Harvey DJ, Dwek RA, Spitsin S, Hanlon CA, Rupprecht C, Dietzschold B, Golovkin M, Koprowski H (2003) Function and glycosylation of plant-derived antiviral monoclonal antibody. Proc Natl Acad Sci USA 100(13):8013\u20138018. https:\/\/doi.org\/10.1073\/pnas.0832472100","journal-title":"Proc Natl Acad Sci USA"},{"issue":"1","key":"433_CR24","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1158\/2326-6066.Cir-16-0194","volume":"5","author":"T Kumai","year":"2017","unstructured":"Kumai T, Lee S, Cho HI, Sultan H, Kobayashi H, Harabuchi Y, Celis E (2017) Optimization of Peptide Vaccines to Induce Robust Antitumor CD4 T-cell Responses. Cancer Immunol Res 5(1):72\u201383. https:\/\/doi.org\/10.1158\/2326-6066.Cir-16-0194","journal-title":"Cancer Immunol Res"},{"key":"433_CR25","doi-asserted-by":"publisher","DOI":"10.5402\/2011\/497397","volume":"1","author":"Y-P Lai","year":"2011","unstructured":"Lai Y-P, Jeng C-J, Chen S-C (2011) The Roles of CD4+ T Cells in Tumor Immunity. Int Sch Res Not 1:497397. https:\/\/doi.org\/10.5402\/2011\/497397","journal-title":"Int Sch Res Not"},{"issue":"4","key":"433_CR26","doi-asserted-by":"publisher","first-page":"345","DOI":"10.4062\/biomolther.2016.126","volume":"25","author":"JH Lee","year":"2017","unstructured":"Lee JH, Ko K (2017) Production of recombinant anti-cancer vaccines in plants. Biomol Ther 25(4):345\u2013353. https:\/\/doi.org\/10.4062\/biomolther.2016.126","journal-title":"Biomol Ther"},{"issue":"4","key":"433_CR27","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1007\/s11816-023-00844-7","volume":"17","author":"CE Lee","year":"2023","unstructured":"Lee CE, Lee JH, Chung HJ, Lee DW, Lim JS, Kim K, Kim JW, Lee YS, Kim KS, Min HJ, Ko K, Myung SC (2023) Expression and in vitro function of anti-PD-L1 human antibody expressed in plant. Plant Biotechnol Rep 17(4):531\u2013539. https:\/\/doi.org\/10.1007\/s11816-023-00844-7","journal-title":"Plant Biotechnol Rep"},{"issue":"3","key":"433_CR28","doi-asserted-by":"publisher","first-page":"264","DOI":"10.7235\/Hort.20240023","volume":"42","author":"Y Lee","year":"2024","unstructured":"Lee Y, Park J, Kim Y, Hwang H, Jin C, Oh Y, Kang Y, Lee D, Song M, Lee Y, Ko K, Hong M (2024) Transient plant expression of recombinant rabies virus glycoprotein: a strategy for vaccine production. Hortic Sci Technol 42(3):264\u2013278. https:\/\/doi.org\/10.7235\/Hort.20240023","journal-title":"Hortic Sci Technol"},{"key":"433_CR29","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00778","author":"CY Lim","year":"2015","unstructured":"Lim CY, Lee KJ, Oh DB, Ko K (2015) Effect of the developmental stage and tissue position on the expression and glycosylation of recombinant glycoprotein GA733-FcK in transgenic plants. Front Plant Sci. https:\/\/doi.org\/10.3389\/fpls.2014.00778","journal-title":"Front Plant Sci"},{"key":"433_CR30","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14552","author":"S Lim","year":"2024","unstructured":"Lim S, Chung HJ, Oh YJ, Hinterdorfer P, Myung SC, Seo Y-J, Ko K (2024) Modification of Fc-fusion protein structures to enhance efficacy of cancer vaccine in plant expression system. Plant Biotechnol J. https:\/\/doi.org\/10.1111\/pbi.14552","journal-title":"Plant Biotechnol J"},{"key":"433_CR31","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/364240","author":"Z Lu","year":"2012","unstructured":"Lu Z, Lee KJ, Shao Y, Lee JH, So Y, Choo YK, Oh DB, Hwang KA, Oh SH, Han YS, Ko K (2012) Expression of GA733-Fc fusion protein as a vaccine candidate for colorectal cancer in transgenic plants. J Biomed Biotechnol. https:\/\/doi.org\/10.1155\/2012\/364240","journal-title":"J Biomed Biotechnol"},{"issue":"14","key":"433_CR32","doi-asserted-by":"publisher","first-page":"6435","DOI":"10.1158\/0008-5472.Can-05-0133","volume":"65","author":"A Machlenkin","year":"2005","unstructured":"Machlenkin A, Paz A, Haim EB, Goldberger O, Finkel E, Tirosh B, Volovitz I, Vadai E, Lugassy G, Cytron S, Lemonnier F, Tzehoval E, Eisenbach L (2005) Human CTL epitopes prostatic acid phosphatase-3 and six-transmembrane epithelial antigen of prostate-3 as candidates for prostate cancer immunotherapy. Cancer Res 65(14):6435\u20136442. https:\/\/doi.org\/10.1158\/0008-5472.Can-05-0133","journal-title":"Cancer Res"},{"issue":"1","key":"433_CR33","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1186\/s12934-021-01617-z","volume":"20","author":"A Madhavan","year":"2021","unstructured":"Madhavan A, Arun KB, Sindhu R, Krishnamoorthy J, Reshmy R, Sirohi R, Pugazhendi A, Awasthi MK, Szakacs G, Binod P (2021) Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up. Microb Cell Fact 20(1):124. https:\/\/doi.org\/10.1186\/s12934-021-01617-z","journal-title":"Microb Cell Fact"},{"issue":"19","key":"433_CR34","doi-asserted-by":"publisher","first-page":"6933","DOI":"10.1158\/1078-0432.Ccr-05-0682","volume":"11","author":"S Matsueda","year":"2005","unstructured":"Matsueda S, Takedatsu H, Yao A, Tanaka M, Noguchi M, Itoh K, Harada M (2005) Identification of peptide vaccine candidates for prostate cancer patients with HLA-A3 supertype alleles. Clin Cancer Res 11(19):6933\u20136943. https:\/\/doi.org\/10.1158\/1078-0432.Ccr-05-0682","journal-title":"Clin Cancer Res"},{"issue":"25","key":"433_CR35","doi-asserted-by":"publisher","first-page":"4047","DOI":"10.1200\/Jco.2008.19.9968","volume":"27","author":"DG McNeel","year":"2009","unstructured":"McNeel DG, Dunphy EJ, Davies JG, Frye TP, Johnson LE, Staab MJ, Horvath DL, Straus J, Alberti D, Marnocha R, Liu G, Eickhoff JC, Wilding G (2009) Safety and immunological efficacy of a DNA vaccine encoding prostatic acid phosphatase in patients with stage D0 prostate cancer. J Clin Oncol 27(25):4047\u20134054. https:\/\/doi.org\/10.1200\/Jco.2008.19.9968","journal-title":"J Clin Oncol"},{"key":"433_CR36","first-page":"179","volume":"135","author":"D Mekhaiel","year":"2011","unstructured":"Mekhaiel D, Czajkowsky D, Andersen JT, Shi J, Sandlie I, McIntosh R, Pleass R (2011) Polymeric human Fc-fusion proteins with modified effector functions. Immunology 135:179\u2013179","journal-title":"Immunology"},{"key":"433_CR37","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.coi.2017.07.004","volume":"47","author":"M Melssen","year":"2017","unstructured":"Melssen M, Slingluff CL Jr (2017) Vaccines targeting helper T cells for cancer immunotherapy. Curr Opin Immunol 47:85\u201392. https:\/\/doi.org\/10.1016\/j.coi.2017.07.004","journal-title":"Curr Opin Immunol"},{"key":"433_CR38","doi-asserted-by":"publisher","first-page":"81","DOI":"10.3389\/fbioe.2018.00081","volume":"6","author":"L Montero-Morales","year":"2018","unstructured":"Montero-Morales L, Steinkellner H (2018) Advanced plant-based glycan engineering. Front Bioeng Biotechnol 6:81. https:\/\/doi.org\/10.3389\/fbioe.2018.00081","journal-title":"Front Bioeng Biotechnol"},{"issue":"5","key":"433_CR39","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1016\/0092-8674(87)90086-9","volume":"48","author":"S Munro","year":"1987","unstructured":"Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal Er proteins. Cell 48(5):899\u2013907. https:\/\/doi.org\/10.1016\/0092-8674(87)90086-9","journal-title":"Cell"},{"issue":"7","key":"433_CR40","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1007\/s00299-023-03028-3","volume":"42","author":"S Oh","year":"2023","unstructured":"Oh S, Kim K, Kang YJ, Hwang H, Kim Y, Hinterdorfer P, Kim MK, Ko K, Lee YK, Kim D, Myung SC, Ko K (2023) Co-transient expression of PSA-Fc and PAP-Fc fusion protein in plant as prostate cancer vaccine candidates and immune responses in mice. Plant Cell Rep 42(7):1203\u20131215. https:\/\/doi.org\/10.1007\/s00299-023-03028-3","journal-title":"Plant Cell Rep"},{"issue":"331","key":"433_CR41","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1093\/jexbot\/50.331.157","volume":"50","author":"S Pagny","year":"1999","unstructured":"Pagny S, Lerouge P, Faye L, Gomord V (1999) Signals and mechanisms for protein retention in the endoplasmic reticulum. J Exp Bot 50(331):157\u2013164. https:\/\/doi.org\/10.1093\/jexbot\/50.331.157","journal-title":"J Exp Bot"},{"key":"433_CR42","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.01040","author":"SR Park","year":"2015","unstructured":"Park SR, Lim CY, Kim DS, Ko K (2015) Optimization of ammonium sulfate concentration for purification of colorectal cancer vaccine candidate recombinant protein GA733-FcK isolated from plants. Front Plant Sci. https:\/\/doi.org\/10.3389\/fpls.2015.01040","journal-title":"Front Plant Sci"},{"issue":"25","key":"433_CR43","doi-asserted-by":"publisher","first-page":"16126","DOI":"10.1021\/acsnano.4c00360","volume":"18","author":"C Park","year":"2024","unstructured":"Park C, Kim K, Kim Y, Zhu R, Hain L, Seferovic H, Kim MH, Woo HJ, Hwang H, Lee SH, Kim S, Lee JE, Hinterdorfer P, Ko K, Park S, Oh YJ (2024) Plant-derived anti-human epidermal growth factor receptor 2 antibody suppresses trastuzumab-resistant breast cancer with enhanced nanoscale binding. ACS Nano 18(25):16126\u201316140. https:\/\/doi.org\/10.1021\/acsnano.4c00360","journal-title":"ACS Nano"},{"issue":"1","key":"433_CR44","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1007\/s11240-012-0141-x","volume":"110","author":"AS Pires","year":"2012","unstructured":"Pires AS, Rosa S, Castanheira S, Fevereiro P, Abranches R (2012) Expression of a recombinant human erythropoietin in suspension cell cultures of Arabidopsis, tobacco and. Plant Cell Tiss Org 110(1):171\u2013181. https:\/\/doi.org\/10.1007\/s11240-012-0141-x","journal-title":"Plant Cell Tiss Org"},{"key":"433_CR45","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1146\/annurev.immunol.19.1.275","volume":"19","author":"JV Ravetch","year":"2001","unstructured":"Ravetch JV, Bolland S (2001) IgG Fc receptors. Annu Rev Immunol 19:275\u2013290. https:\/\/doi.org\/10.1146\/annurev.immunol.19.1.275","journal-title":"Annu Rev Immunol"},{"issue":"3","key":"433_CR46","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1002\/pros.20135","volume":"62","author":"AK Roos","year":"2005","unstructured":"Roos AK, Pavlenko M, Charo J, Egevad L, Pisa P (2005) Induction of PSA-specific CTLs and anti-tumor immunity by a genetic prostate cancer vaccine. Prostate 62(3):217\u2013223. https:\/\/doi.org\/10.1002\/pros.20135","journal-title":"Prostate"},{"issue":"4","key":"433_CR47","doi-asserted-by":"publisher","first-page":"994","DOI":"10.1002\/eji.201343863","volume":"44","author":"JMS Saif","year":"2014","unstructured":"Saif JMS, Vadakekolathu J, Rane SS, McDonald D, Ahmad M, Mathieu M, Pockley AG, Durrant L, Metheringham R, Rees RC, McArdle SEB (2014) Novel prostate acid phosphatase-based peptide vaccination strategy induces antigen-specific T-cell responses and limits tumour growth in mice. Eur J Immunol 44(4):994\u20131004. https:\/\/doi.org\/10.1002\/eji.201343863","journal-title":"Eur J Immunol"},{"issue":"4","key":"433_CR48","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/j.jmb.2012.01.027","volume":"417","author":"JV Schaefer","year":"2012","unstructured":"Schaefer JV, Pl\u00fcckthun A (2012) Engineering aggregation resistance in IgG by two independent mechanisms: lessons from comparison of Phichia pastoris and mammalian cell expression. J Mol Biol 417(4):309\u2013335. https:\/\/doi.org\/10.1016\/j.jmb.2012.01.027","journal-title":"J Mol Biol"},{"issue":"4","key":"433_CR49","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1007\/Bf00019011","volume":"30","author":"A Schouten","year":"1996","unstructured":"Schouten A, Roosien J, vanEngelen FA, deJong GAM, BorstVrenssen AWM, Zilverentant JF, Bosch D, Stiekema WJ, Gommers FJ, Schots A, Bakker J (1996) The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco. Plant Mol Biol 30(4):781\u2013793. https:\/\/doi.org\/10.1007\/Bf00019011","journal-title":"Plant Mol Biol"},{"key":"433_CR50","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jconrel.2023.05.030","volume":"359","author":"AM Shen","year":"2023","unstructured":"Shen AM, Malekshah OM, Pogrebnyak N, Minko T (2023) Plant-derived single domain COVID-19 antibodies. J Control Release 359:1\u201311. https:\/\/doi.org\/10.1016\/j.jconrel.2023.05.030","journal-title":"J Control Release"},{"issue":"2","key":"433_CR51","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1080\/19768354.2019.1575904","volume":"23","author":"C Shin","year":"2019","unstructured":"Shin C, Kang Y, Kim HS, Shin YK, Ko K (2019) Immune response of heterologous recombinant antigenic protein of viral hemorrhagic septicemia virus (VHSV) in mice. Anim Cells Syst 23(2):97\u2013105. https:\/\/doi.org\/10.1080\/19768354.2019.1575904","journal-title":"Anim Cells Syst"},{"issue":"1","key":"433_CR52","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1007\/s11240-012-0249-z","volume":"113","author":"Y So","year":"2013","unstructured":"So Y, Lee KJ, Kim DS, Lee JH, Oh DB, Hwang KA, Ko K, Choo YK, Ko K (2013) Glycomodification and characterization of anti-colorectal cancer immunotherapeutic monoclonal antibodies in transgenic tobacco. Plant Cell Tissue Org 113(1):41\u201349. https:\/\/doi.org\/10.1007\/s11240-012-0249-z","journal-title":"Plant Cell Tissue Org"},{"key":"433_CR53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0198978","author":"I Song","year":"2018","unstructured":"Song I, Kang Y, Lee YK, Myung SC, Ko K (2018) Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response. PLoS ONE. https:\/\/doi.org\/10.1371\/journal.pone.0198978","journal-title":"PLoS ONE"},{"key":"433_CR54","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.6828","author":"I Song","year":"2019","unstructured":"Song I, Kang YJ, Choi SL, Han D, Kim DS, Lee HK, Lee JC, Park J, Kim DS, Ko K (2019) Purification of plant-derived anti-virus mAb through optimized pH conditions for coupling between protein A and epoxy-activated beads. PeerJ. https:\/\/doi.org\/10.7717\/peerj.6828","journal-title":"PeerJ"},{"issue":"11","key":"433_CR55","doi-asserted-by":"publisher","first-page":"3332","DOI":"10.4161\/21645515.2014.973317","volume":"10","author":"M Tagliamonte","year":"2014","unstructured":"Tagliamonte M, Petrizzo A, Tornesello ML, Buonaguro FM, Buonaguro L (2014) Antigen-specific vaccines for cancer treatment. Hum Vaccin Immunother 10(11):3332\u20133346. https:\/\/doi.org\/10.4161\/21645515.2014.973317","journal-title":"Hum Vaccin Immunother"},{"issue":"5","key":"433_CR56","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1634\/theoncologist.11-5-451","volume":"11","author":"CP Tarassoff","year":"2006","unstructured":"Tarassoff CP, Arlen PM, Gulley JL (2006) Therapeutic vaccines for prostate cancer. Oncologist 11(5):451\u2013462. https:\/\/doi.org\/10.1634\/theoncologist.11-5-451","journal-title":"Oncologist"},{"issue":"12","key":"433_CR57","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1016\/j.tibtech.2003.10.002","volume":"21","author":"RM Twyman","year":"2003","unstructured":"Twyman RM, Stoger E, Schillberg S, Christou P, Fischer R (2003) Molecular farming in plants: host systems and expression technology. Trends Biotechnol 21(12):570\u2013578. https:\/\/doi.org\/10.1016\/j.tibtech.2003.10.002","journal-title":"Trends Biotechnol"},{"issue":"14","key":"433_CR58","doi-asserted-by":"publisher","first-page":"1058","DOI":"10.1056\/Nejm200104053441406","volume":"344","author":"MJ Walport","year":"2001","unstructured":"Walport MJ (2001) Advances in immunology: complement (first of two parts). New Engl J Med 344(14):1058\u20131066. https:\/\/doi.org\/10.1056\/Nejm200104053441406","journal-title":"New Engl J Med"},{"issue":"2","key":"433_CR59","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1046\/j.1365-313X.1992.t01-41-00999.x","volume":"2","author":"CI Wandelt","year":"1992","unstructured":"Wandelt CI, Khan MRI, Craig S, Schroeder HE, Spencer D, Higgins TJV (1992) Vicilin with carboxy-terminal Kdel Is retained in the endoplasmic-reticulum and accumulates to high-levels in the leaves of transgenic plants. Plant J 2(2):181\u2013192","journal-title":"Plant J"},{"key":"433_CR60","doi-asserted-by":"publisher","first-page":"191","DOI":"10.3389\/fimmu.2014.00191","volume":"5","author":"H Westdorp","year":"2014","unstructured":"Westdorp H, Skold AE, Snijer BA, Franik S, Mulder SF, Major PP, Foley R, Gerritsen WR, de Vries IJ (2014) Immunotherapy for prostate cancer: lessons from responses to tumor-associated antigens. Front Immunol 5:191. https:\/\/doi.org\/10.3389\/fimmu.2014.00191","journal-title":"Front Immunol"},{"issue":"2","key":"433_CR61","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1038\/nri1266","volume":"4","author":"JM Woof","year":"2004","unstructured":"Woof JM, Burton DR (2004) Human antibody\u2014Fc receptor interactions illuminated by crystal structures. Nat Rev Immunol 4(2):89\u201399. https:\/\/doi.org\/10.1038\/nri1266","journal-title":"Nat Rev Immunol"},{"key":"433_CR62","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01860","author":"CP Yang","year":"2018","unstructured":"Yang CP, Gao XY, Gong R (2018) Engineering of Fc fragments with optimized physicochemical properties implying improvement of clinical potentials for Fc-based therapeutics. Front Immunol. https:\/\/doi.org\/10.3389\/fimmu.2017.01860","journal-title":"Front Immunol"},{"issue":"5","key":"433_CR63","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1002\/stem.5530120502","volume":"12","author":"SF Ziegler","year":"1994","unstructured":"Ziegler SF, Ramsdell F, Alderson MR (1994) The activation antigen CD69. Stem Cells 12(5):456\u2013465. https:\/\/doi.org\/10.1002\/stem.5530120502","journal-title":"Stem Cells"}],"container-title":["Transgenic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11248-025-00433-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11248-025-00433-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11248-025-00433-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T18:52:21Z","timestamp":1743274341000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11248-025-00433-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,26]]},"references-count":63,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["433"],"URL":"https:\/\/doi.org\/10.1007\/s11248-025-00433-0","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-5286242\/v1","asserted-by":"object"}]},"ISSN":["0962-8819","1573-9368"],"issn-type":[{"value":"0962-8819","type":"print"},{"value":"1573-9368","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,26]]},"assertion":[{"value":"18 October 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 February 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 March 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no competing financial interest","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"16"}}