{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T01:34:16Z","timestamp":1775784856165,"version":"3.50.1"},"reference-count":46,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["440533\/2022-8"],"award-info":[{"award-number":["440533\/2022-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Transgenic Res"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11248-025-00440-1","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T13:22:57Z","timestamp":1744377777000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Enhanced folate content in bioengineered lettuce stably expressed under field conditions"],"prefix":"10.1007","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5339-5793","authenticated-orcid":false,"given":"L\u00eddia N.","family":"Queiroz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6735-8771","authenticated-orcid":false,"given":"Tatiane R.","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7430-1573","authenticated-orcid":false,"given":"Ana C. M. M.","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6799-4706","authenticated-orcid":false,"given":"Glaucia B.","family":"Cabral","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0570-7673","authenticated-orcid":false,"given":"Francisco J. L.","family":"Arag\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"440_CR1","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.arr.2015.04.005","volume":"22","author":"JR Ara\u00fajo","year":"2015","unstructured":"Ara\u00fajo JR, Martel F, Borges N, Ara\u00fajo JM, Keating E (2015) Folates and aging: role in mild cognitive impairment, dementia and depression. Ageing Res Rev 22:9\u201319. https:\/\/doi.org\/10.1016\/j.arr.2015.04.005","journal-title":"Ageing Res Rev"},{"issue":"7","key":"440_CR2","doi-asserted-by":"publisher","first-page":"2661","DOI":"10.1105\/tpc.113.113118","volume":"25","author":"U Armbruster","year":"2013","unstructured":"Armbruster U, Labs M, Pribil M, Viola S, Xu W, Scharfenberg M, Hertle AP, Rojahn U, Jensen PE, Rappaport F, Joliot P, D\u00f6rmann P, Wanner G, Leister D (2013) Arabidopsis CURVATURE THYLAKOID1 proteins modify thylakoid architecture by inducing membrane curvature. Plant Cell 25(7):2661\u20132678. https:\/\/doi.org\/10.1105\/tpc.113.113118","journal-title":"Plant Cell"},{"issue":"1","key":"440_CR3","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1111\/nyas.13511","volume":"1414","author":"LB Bailey","year":"2018","unstructured":"Bailey LB, Hausman DB (2018) Folate status in women of reproductive age as basis of neural tube defect risk assessment. Ann New York Acad Sci 1414(1):82\u201395. https:\/\/doi.org\/10.1111\/nyas.13511","journal-title":"Ann New York Acad Sci"},{"key":"440_CR4","doi-asserted-by":"publisher","first-page":"12489","DOI":"10.1073\/pnas.192278499","volume":"99","author":"G Basset","year":"2002","unstructured":"Basset G, Quinlivan EP, Ziemak MJ, Diaz De La Garza R, Fischer M, Schiffmann S, Bacher A, Gregory JF 3rd, Hanson AD (2002) Folate synthesis in plants: the first step of the pterin branch is mediated by a unique bimodular GTP cyclohydrolase I. PNAS 99:12489\u201312494. https:\/\/doi.org\/10.1073\/pnas.192278499","journal-title":"PNAS"},{"issue":"1","key":"440_CR5","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.tplants.2007.11.001","volume":"13","author":"S Bekaert","year":"2008","unstructured":"Bekaert S, Storozhenko S, Mehrshahi P, Bennett MJ, Lambert W, Gregory JF 3rd, Schubert K, Hugenholtz J, Van Der Straeten D, Hanson AD (2008) Folate biofortification in food plants. Trends Plant Sci 13(1):28\u201335. https:\/\/doi.org\/10.1016\/j.tplants.2007.11.001","journal-title":"Trends Plant Sci"},{"key":"440_CR6","doi-asserted-by":"publisher","first-page":"1743","DOI":"10.1007\/s00381-023-05837-z","volume":"39","author":"A Benavides-Lara","year":"2023","unstructured":"Benavides-Lara A, Fern\u00e1ndez-S\u00e1nchez O, Barboza-Arg\u00fcello M et al (2023) Integrated surveillance strategy to support the prevention of neural tube defects through food fortification with folic acid: the experience of Costa Rica. Childs Nerv Syst 39:1743\u20131754. https:\/\/doi.org\/10.1007\/s00381-023-05837-z","journal-title":"Childs Nerv Syst"},{"key":"440_CR7","doi-asserted-by":"publisher","first-page":"1048","DOI":"10.1111\/ppa.13539","volume":"71","author":"PS Berbert","year":"2022","unstructured":"Berbert PS, Vieira PM, Cabral GB, Martins EC, Wulff NA, Ulhoa CJ, Arag\u00e3o FJL (2022) Expression of a sphingomyelinase-coding gene from Trichoderma harzianum conferred bacterial tolerance in tobacco. Plant Pathol 71:1048\u20131055. https:\/\/doi.org\/10.1111\/ppa.13539","journal-title":"Plant Pathol"},{"issue":"12","key":"440_CR8","doi-asserted-by":"publisher","first-page":"3899","DOI":"10.1093\/jxb\/ert224","volume":"64","author":"D Blancquaert","year":"2013","unstructured":"Blancquaert D, Storozhenko S, Van Daele J, Stove C, Visser RG, Lambert W, Van Der Straeten D (2013) Enhancing pterin and para-aminobenzoate content is not sufficient to successfully biofortify potato tubers and Arabidopsis thaliana plants with folate. J Exp Bot 64(12):3899\u20133909. https:\/\/doi.org\/10.1093\/jxb\/ert224","journal-title":"J Exp Bot"},{"key":"440_CR100","doi-asserted-by":"publisher","unstructured":"Blancquaert D, De Steur H, Gellynck X, Van Der Straeten D (2014) Present and future of folate biofortification of crop plants. J Exp Bot 65:895\u2013906. https:\/\/doi.org\/10.1093\/jxb\/ert483","DOI":"10.1093\/jxb\/ert483"},{"issue":"1","key":"440_CR9","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1111\/nyas.13274","volume":"1390","author":"D Blancquaert","year":"2017","unstructured":"Blancquaert D, De Steur H, Gellynck X, Van Der Straeten D (2017) Metabolic engineering of micronutrients in crop plants. Ann New York Acad Sci 1390(1):59\u201373. https:\/\/doi.org\/10.1111\/nyas.13274","journal-title":"Ann New York Acad Sci"},{"issue":"4","key":"440_CR10","doi-asserted-by":"publisher","first-page":"247","DOI":"10.3746\/pnf.2014.19.4.247","volume":"19","author":"JH Choi","year":"2014","unstructured":"Choi JH, Yates Z, Veysey M, Heo YR, Lucock M (2014) Contemporary issues surrounding folic Acid fortification initiatives. Prev Nut Food Sci 19(4):247\u2013260. https:\/\/doi.org\/10.3746\/pnf.2014.19.4.247","journal-title":"Prev Nut Food Sci"},{"key":"440_CR11","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1146\/annurev-nutr-043020-091647","volume":"42","author":"KS Crider","year":"2022","unstructured":"Crider KS, Qi YP, Yeung LF, Mai CT, Head Zauche L, Wang A, Daniels K, Williams JL (2022) Folic acid and the prevention of birth defects: 30 years of opportunity and controversies. Ann Rev Nut 42:423\u2013452. https:\/\/doi.org\/10.1146\/annurev-nutr-043020-091647","journal-title":"Ann Rev Nut"},{"issue":"1","key":"440_CR12","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/j.molp.2017.12.008","volume":"11","author":"J De Lepeleire","year":"2018","unstructured":"De Lepeleire J, Strobbe S, Verstraete J, Blancquaert D, Ambach L, Visser RGF, Stove C, Van Der Straeten D (2018) Folate biofortification of potato by tuber-specific expression of four folate biosynthesis genes. Mol Plant 11(1):175\u2013188. https:\/\/doi.org\/10.1016\/j.molp.2017.12.008","journal-title":"Mol Plant"},{"key":"440_CR13","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1111\/j.1365-3059.2006.01342.x","volume":"55","author":"BBA Dias","year":"2006","unstructured":"Dias BBA, Cunha WG, Morais LS, Vianna GR, Rech EL, de Capdeville G, Arag\u00e3o FJL (2006) Expression of an oxalate decarboxylase gene from Flammulina sp. in transgenic lettuce (Lactuca sativa) plants and resistance to Sclerotinia sclerotiorum. Plant Pathol 55:187\u2013193. https:\/\/doi.org\/10.1111\/j.1365-3059.2006.01342.x","journal-title":"Plant Pathol"},{"key":"440_CR15","doi-asserted-by":"publisher","first-page":"4218","DOI":"10.1073\/pnas.0700409104","volume":"104","author":"RI D\u00edaz de la Garza","year":"2007","unstructured":"D\u00edaz de la Garza RI, Gregory JF 3rd, Hanson AD (2007) Folate biofortification of tomato fruit. PNAS 104:4218\u20134222. https:\/\/doi.org\/10.1073\/pnas.0700409104","journal-title":"PNAS"},{"key":"440_CR16","doi-asserted-by":"publisher","first-page":"1080188","DOI":"10.3389\/fendo.2023.1080188","volume":"14","author":"X Feng","year":"2023","unstructured":"Feng X, Mei Y, Xie P, Xing Z, Wang X, Cui L, Xu R (2023) Serum folic acid: an effective indicator for arteriogenic erectile dysfunction. Front Endocrinol 14:1080188. https:\/\/doi.org\/10.3389\/fendo.2023.1080188","journal-title":"Front Endocrinol"},{"key":"440_CR17","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1097\/00005237-200310000-00005","volume":"17","author":"J Geisel","year":"2003","unstructured":"Geisel J (2003) Folic acid and neural tube defects in pregnancy: a review. J Perinat Neonat Nurs 17:268\u2013279. https:\/\/doi.org\/10.1097\/00005237-200310000-00005","journal-title":"J Perinat Neonat Nurs"},{"key":"440_CR18","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/s1369-5266(02)00249-2","volume":"5","author":"AD Hanson","year":"2002","unstructured":"Hanson AD, Gregory JF 3rd (2002) Synthesis and turnover of folates in plants. Curr Opin Plant Bio 5:244\u2013249. https:\/\/doi.org\/10.1016\/s1369-5266(02)00249-2","journal-title":"Curr Opin Plant Bio"},{"key":"440_CR19","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1146\/annurev-arplant-042110-103819","volume":"62","author":"AD Hanson","year":"2011","unstructured":"Hanson AD, Gregory JF 3rd (2011) Folate biosynthesis, turnover, and transport in plants. Ann Rev Plant Bio 62:105\u2013125. https:\/\/doi.org\/10.1146\/annurev-arplant-042110-103819","journal-title":"Ann Rev Plant Bio"},{"key":"440_CR20","doi-asserted-by":"publisher","first-page":"5158","DOI":"10.1073\/pnas.0401342101","volume":"101","author":"T Hossain","year":"2004","unstructured":"Hossain T, Rosenberg I, Selhub J, Kishore G, Beachy R, Schubert K (2004) Enhancement of folates in plants through metabolic engineering. PNAS 101:5158\u20135163. https:\/\/doi.org\/10.1073\/pnas.0401342101","journal-title":"PNAS"},{"issue":"9","key":"440_CR21","doi-asserted-by":"publisher","first-page":"4352","DOI":"10.3390\/ijerph10094352","volume":"10","author":"A Imbard","year":"2013","unstructured":"Imbard A, Benoist JF, Blom HJ (2013) Neural tube defects, folic acid and methylation. Int J Env Res Pub Health 10(9):4352\u20134389. https:\/\/doi.org\/10.3390\/ijerph10094352","journal-title":"Int J Env Res Pub Health"},{"key":"440_CR22","unstructured":"National Institutes of Health (USA): Office of Dietary Supplements.\u00a0Folate: Fact sheet for consumers. https:\/\/ods.od.nih.gov\/factsheets\/Folate-Consumer\/ Accessed 6 Nov 2024."},{"key":"440_CR23","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1002\/9780470686522.ch14","volume-title":"Plant Cell Culture","author":"C Lacorte","year":"2010","unstructured":"Lacorte C, Vianna G, Arag\u00e3o FJL, Rech EL (2010) Molecular Characterization of Genetically Manipulated Plants. In: Davey MR, Anthony P (eds) Plant Cell Culture, 1st edn. Wiley, New York, pp 261\u2013279","edition":"1"},{"key":"440_CR24","doi-asserted-by":"publisher","first-page":"199264","DOI":"10.1016\/j.virusres.2023.199264","volume":"339","author":"MO Leastro","year":"2024","unstructured":"Leastro MO, Kitajima EW, Pall\u00e1s V, S\u00e1nchez-Navarro J\u00c1 (2024) Rescue of a Cilevirus from infectious cDNA clones. Virus Res 339:199264. https:\/\/doi.org\/10.1016\/j.virusres.2023.199264","journal-title":"Virus Res"},{"key":"440_CR25","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.1093\/jxb\/ery453","volume":"70","author":"Q Liang","year":"2019","unstructured":"Liang Q, Wang K, Liu X, Riaz B, Jiang L, Wan X, Ye X, Zhang C (2019) Improved folate accumulation in genetically modified maize and wheat. J Exp Bot 70:1539\u20131551. https:\/\/doi.org\/10.1093\/jxb\/ery453","journal-title":"J Exp Bot"},{"key":"440_CR26","doi-asserted-by":"publisher","first-page":"127459","DOI":"10.1016\/j.foodchem.2020.127459","volume":"333","author":"Q Liang","year":"2020","unstructured":"Liang Q, Wang K, Shariful I, Ye X, Zhang C (2020) Folate content and retention in wheat grains and wheat-based foods: Effects of storage, processing, and cooking methods. Food Chem 333:127459. https:\/\/doi.org\/10.1016\/j.foodchem.2020.127459","journal-title":"Food Chem"},{"key":"440_CR27","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1046\/j.1365-313x.1995.08030457.x","volume":"8","author":"YG Liu","year":"1995","unstructured":"Liu YG, Mitsukawa N, Oosumi T, Whittier RF (1995) Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J Cell Mol Bio 8:457\u2013463. https:\/\/doi.org\/10.1046\/j.1365-313x.1995.08030457.x","journal-title":"Plant J Cell Mol Bio"},{"key":"440_CR28","doi-asserted-by":"publisher","DOI":"10.1007\/s11240-024-02796-4","author":"TR Monteiro","year":"2024","unstructured":"Monteiro TR, Queiroz LN, Cabral GB, Citadin CT, Santos MP, Arag\u00e3o FJL (2024) Evaluation of two new promoters to express transgenes stably in lettuce (Lactuca sativa L.). Plant Cell Tissue Organ Culture (PCTOC). https:\/\/doi.org\/10.1007\/s11240-024-02796-4","journal-title":"Plant Cell Tissue Organ Culture (PCTOC)"},{"key":"440_CR29","doi-asserted-by":"publisher","first-page":"7762","DOI":"10.1073\/pnas.0901412106","volume":"106","author":"S Naqvi","year":"2009","unstructured":"Naqvi S, Zhu C, Farre G, Ramessar K, Bassie L, Breitenbach J, Perez Conesa D, Ros G, Sandmann G, Capell T, Christou P (2009) Transgenic multivitamin corn through biofortification of endosperm with three vitamins representing three distinct metabolic pathways. PNAS 106:7762\u20137767. https:\/\/doi.org\/10.1073\/pnas.0901412106","journal-title":"PNAS"},{"key":"440_CR30","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1007\/s11248-009-9256-1","volume":"18","author":"AC Nunes","year":"2009","unstructured":"Nunes AC, Kalkmann DC, Arag\u00e3o FJL (2009) Folate biofortification of lettuce by expression of a codon optimized chicken GTP cyclohydrolase I gene. Transgenic Res 18:661\u2013667. https:\/\/doi.org\/10.1007\/s11248-009-9256-1","journal-title":"Transgenic Res"},{"key":"440_CR31","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1007\/s00299-015-1865-y","volume":"35","author":"A Okumura","year":"2016","unstructured":"Okumura A, Shimada A, Yamasaki S, Horino T, Iwata Y, Koizumi N, Nishihara M, Mishiba K (2016) CaMV-35S promoter sequence-specific DNA methylation in lettuce. Plant Cell Rep 35:43\u201351. https:\/\/doi.org\/10.1007\/s00299-015-1865-y","journal-title":"Plant Cell Rep"},{"key":"440_CR32","doi-asserted-by":"publisher","first-page":"285S","DOI":"10.1093\/ajcn\/85.1.285S","volume":"85","author":"RM Pitkin","year":"2007","unstructured":"Pitkin RM (2007) Folate and neural tube defects. Am J Clin Nut 85:285S-288S. https:\/\/doi.org\/10.1093\/ajcn\/85.1.285S","journal-title":"Am J Clin Nut"},{"key":"440_CR33","doi-asserted-by":"publisher","first-page":"2351","DOI":"10.1104\/pp.17.00863","volume":"176","author":"M Pribil","year":"2018","unstructured":"Pribil M, Sandoval-Ib\u00e1\u00f1ez O, Xu W, Sharma A, Labs M, Liu Q, Galgenm\u00fcller C, Schneider T, Wessels M, Matsubara S, Jansson S, Wanner G, Leister D (2018) Fine-tuning of photosynthesis requires CURVATURE THYLAKOID1-mediated thylakoid plasticity. Plant Physiol 176:2351\u20132364. https:\/\/doi.org\/10.1104\/pp.17.00863","journal-title":"Plant Physiol"},{"key":"440_CR34","doi-asserted-by":"publisher","first-page":"2021","DOI":"10.1111\/pbi.12561","volume":"14","author":"NGR Rivera","year":"2016","unstructured":"Rivera NGR, Garc\u00eda-Salinas C, Arag\u00e3o FJL, D\u00edaz de la Garza RI (2016) Metabolic engineering of folate and its precursors in Mexican common bean (Phaseolus vulgaris L.). Plant Biotech J 14:2021\u20132032. https:\/\/doi.org\/10.1111\/pbi.12561","journal-title":"Plant Biotech J"},{"issue":"2","key":"440_CR35","doi-asserted-by":"publisher","first-page":"e0281077","DOI":"10.1371\/journal.pone.0281077","volume":"18","author":"VB Rodrigues","year":"2023","unstructured":"Rodrigues VB, Silva ENd, dos Santos AM, Santos LMP (2023) Prevented cases of neural tube defects and cost savings after folic acid fortification of flour in Brazil. PLoS ONE 18(2):e0281077. https:\/\/doi.org\/10.1371\/journal.pone.0281077","journal-title":"PLoS ONE"},{"key":"440_CR36","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1111\/nyas.13314","volume":"1390","author":"A Saltzman","year":"2017","unstructured":"Saltzman A, Birol E, Oparinde A, Andersson MS, Asare-Marfo D, Diressie MT, Gonzalez C, Lividini K, Moursi M, Zeller M (2017) Availability, production, and consumption of crops biofortified by plant breeding: current evidence and future potential. Ann New York Acad Sci 1390:104\u2013114. https:\/\/doi.org\/10.1111\/nyas.13314","journal-title":"Ann New York Acad Sci"},{"key":"440_CR37","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2113934118","author":"O Sandoval-Ib\u00e1\u00f1ez","year":"2021","unstructured":"Sandoval-Ib\u00e1\u00f1ez O, Sharma A, Bykowski M, Borr\u00e0s-Gas G, Behrendorff JBYH, Mellor S, Qvortrup K, Verdonk JC, Bock R, Kowalewska \u0141, Pribil M (2021) Curvature thylakoid 1 proteins modulate prolamellar body morphology and promote organized thylakoid biogenesis in\u00a0Arabidopsis thaliana. PNAS. https:\/\/doi.org\/10.1073\/pnas.2113934118","journal-title":"PNAS"},{"key":"440_CR38","doi-asserted-by":"publisher","first-page":"22","DOI":"10.2471\/BLT.14.151365","volume":"94","author":"LM Santos","year":"2016","unstructured":"Santos LM, Lecca RC, Cortez-Escalante JJ, Sanchez MN, Rodrigues HG (2016) Prevention of neural tube defects by the fortification of flour with folic acid: a population-based retrospective study in Brazil. Bull World Health Organ 94:22\u201329. https:\/\/doi.org\/10.2471\/BLT.14.151365","journal-title":"Bull World Health Organ"},{"key":"440_CR39","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1002\/(SICI)1097-0010(20000515)80:7<795::AID-JSFA599>3.0.CO;2-K","volume":"80","author":"J Scot","year":"2000","unstructured":"Scot J, R\u00e9beill\u00e9 F, Fletcher J (2000) Folic acid and folates: the feasibility for nutritional enhancement in plant foods. J Sci Food Agric 80:795\u2013824. https:\/\/doi.org\/10.1002\/(SICI)1097-0010(20000515)80:7%3c795::AID-JSFA599%3e3.0.CO;2-K","journal-title":"J Sci Food Agric"},{"key":"440_CR40","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1093\/ajcn\/85.1.3","volume":"85","author":"AD Smith","year":"2007","unstructured":"Smith AD (2007) Folic acid fortification: the good, the bad, and the puzzle of vitamin B-12. Am J Clin Nut 85:3\u20135. https:\/\/doi.org\/10.1093\/ajcn\/85.1.3","journal-title":"Am J Clin Nut"},{"key":"440_CR41","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1016\/j.clnu.2016.09.032","volume":"36","author":"P Soeters","year":"2017","unstructured":"Soeters P, Bozzetti F, Cynober L, Forbes A, Shenkin A, Sobotka L (2017) Defining malnutrition: a plea to rethink. Clin Nut 36:896\u2013901. https:\/\/doi.org\/10.1016\/j.clnu.2016.09.032","journal-title":"Clin Nut"},{"key":"440_CR42","doi-asserted-by":"publisher","first-page":"1277","DOI":"10.1038\/nbt1351","volume":"25","author":"S Storozhenko","year":"2007","unstructured":"Storozhenko S, De Brouwer V, Volckaert M, Navarrete O, Blancquaert D, Zhang GF, Lambert W, Van Der Straeten D (2007) Folate fortification of rice by metabolic engineering. Nat Biotech 25:1277\u20131279. https:\/\/doi.org\/10.1038\/nbt1351","journal-title":"Nat Biotech"},{"key":"440_CR43","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/j.copbio.2016.12.003","volume":"44","author":"S Strobbe","year":"2017","unstructured":"Strobbe S, Van Der Straeten D (2017) Folate biofortification in food crops. Curr Opin Biotech 44:202\u2013211. https:\/\/doi.org\/10.1016\/j.copbio.2016.12.003","journal-title":"Curr Opin Biotech"},{"key":"440_CR44","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1056\/NEJMp038186","volume":"350","author":"NJ Wald","year":"2004","unstructured":"Wald NJ (2004) Folic acid and the prevention of neural-tube defects. New Eng J Med 350:101\u2013103. https:\/\/doi.org\/10.1056\/NEJMp038186","journal-title":"New Eng J Med"},{"key":"440_CR46","first-page":"1","volume":"64","author":"J Williams","year":"2015","unstructured":"Williams J, Mai CT, Mulinare J, Isenburg J, Flood TJ, Ethen M, Frohnert B, Kirby RS (2015) Centers for disease control and prevention. updated estimates of neural tube defects prevented by mandatory folic Acid fortification-United States, 1995\u20132011. MMWR Morb Mortal Wkly Rep 64:1\u20135","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"440_CR47","doi-asserted-by":"publisher","first-page":"100017","DOI":"10.1016\/j.xplc.2019.100017","volume":"1","author":"Q Zhu","year":"2019","unstructured":"Zhu Q, Wang B, Tan J, Liu T, Li L, Liu YG (2019) Plant synthetic metabolic engineering for enhancing crop nutritional quality. Plant Comm 1:100017. https:\/\/doi.org\/10.1016\/j.xplc.2019.100017","journal-title":"Plant Comm"}],"container-title":["Transgenic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11248-025-00440-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11248-025-00440-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11248-025-00440-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T13:22:58Z","timestamp":1744377778000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11248-025-00440-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,11]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["440"],"URL":"https:\/\/doi.org\/10.1007\/s11248-025-00440-1","relation":{},"ISSN":["0962-8819","1573-9368"],"issn-type":[{"value":"0962-8819","type":"print"},{"value":"1573-9368","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,11]]},"assertion":[{"value":"18 January 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interest"}}],"article-number":"20"}}