{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T11:10:07Z","timestamp":1751454607731,"version":"3.41.0"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"China National Biotec Group Company Ltd","award":["CNBG-CD-029"],"award-info":[{"award-number":["CNBG-CD-029"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Transgenic Res"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11248-025-00452-x","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T10:35:29Z","timestamp":1751452529000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Competent expression of effective and long-acting human \u03b1-Gal A-Fc fusion protein in the milk of transgenic mice"],"prefix":"10.1007","volume":"34","author":[{"given":"Mengke","family":"Yuan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiqiang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhipeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianfei","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinzong","family":"Rong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cong","family":"Cong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongxia","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoping","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yali","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengli","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanjun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonghong","family":"Ge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,2]]},"reference":[{"key":"452_CR1","doi-asserted-by":"publisher","first-page":"3046","DOI":"10.1111\/1750-3841.15794","volume":"86","author":"MH Alu\u2019datt","year":"2021","unstructured":"Alu\u2019datt MH, Al-U\u2019datt DGF, Alhamad MN, Tranchant CC, Rababah T, Gammoh S, Althnaibat RM, Daradkeh MG, Kubow S (2021) Characterization and biological properties of peptides isolated from dried fermented cow milk products by RP-HPLC: amino acid composition, antioxidant, antihypertensive, and antidiabetic properties. J Food Sci 86:3046\u20133060. https:\/\/doi.org\/10.1111\/1750-3841.15794","journal-title":"J Food Sci"},{"key":"452_CR2","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1007\/s10545-017-0098-3","volume":"41","author":"AB Burlina","year":"2018","unstructured":"Burlina AB, Polo G, Salviati L, Duro G, Zizzo C, Dardis A, Bembi B, Cazzorla C, Rubert L, Zordan R et al (2018) Newborn screening for lysosomal storage disorders by tandem mass spectrometry in North East Italy. J Inherit Metab Dis 41:209\u2013219. https:\/\/doi.org\/10.1007\/s10545-017-0098-3","journal-title":"J Inherit Metab Dis"},{"key":"452_CR3","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1016\/j.yexcr.2011.02.013","volume":"317","author":"PJ Carter","year":"2011","unstructured":"Carter PJ (2011) Introduction to current and future protein therapeutics: a protein engineering perspective. Exp Cell Res 317:1261\u20131269. https:\/\/doi.org\/10.1016\/j.yexcr.2011.02.013","journal-title":"Exp Cell Res"},{"key":"452_CR4","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252111376","author":"W Dai","year":"2024","unstructured":"Dai W, Han B, Sun Y, Hou P, Wang C, Li W, Liu H (2024) Integrated transcriptomic analyses of liver and mammary gland tissues reveals the regulatory mechanism underlying dairy goats at late lactation when feeding rumen-protected lysine. Int J Mol Sci. https:\/\/doi.org\/10.3390\/ijms252111376","journal-title":"Int J Mol Sci"},{"key":"452_CR5","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1016\/j.jmb.2004.01.035","volume":"337","author":"SC Garman","year":"2004","unstructured":"Garman SC, Garboczi DN (2004) The molecular defect leading to Fabry disease: structure of human alpha-galactosidase. J Mol Biol 337:319\u2013335. https:\/\/doi.org\/10.1016\/j.jmb.2004.01.035","journal-title":"J Mol Biol"},{"key":"452_CR6","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1038\/nbt0797-637","volume":"15","author":"V Ghetie","year":"1997","unstructured":"Ghetie V, Popov S, Borvak J, Radu C, Matesoi D, Medesan C, Ober RJ, Ward ES (1997) Increasing the serum persistence of an IgG fragment by random mutagenesis. Nat Biotechnol 15:637\u2013640. https:\/\/doi.org\/10.1038\/nbt0797-637","journal-title":"Nat Biotechnol"},{"key":"452_CR7","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/s1140-4639(05)80168-6","volume":"36","author":"LM Houdebine","year":"1993","unstructured":"Houdebine LM (1993) Expression of recombinant proteins in the milk of transgenic animals. Rev Fr Transfus Hemobiol 36:49\u201372. https:\/\/doi.org\/10.1016\/s1140-4639(05)80168-6","journal-title":"Rev Fr Transfus Hemobiol"},{"key":"452_CR8","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1016\/j.tcm.2017.10.001","volume":"28","author":"TR Hsu","year":"2018","unstructured":"Hsu TR, Niu DM (2018) Fabry disease: review and experience during newborn screening. Trends Cardiovasc Med 28:274\u2013281. https:\/\/doi.org\/10.1016\/j.tcm.2017.10.001","journal-title":"Trends Cardiovasc Med"},{"key":"452_CR9","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1007\/s00428-008-0586-9","volume":"452","author":"J Keslova-Veselikova","year":"2008","unstructured":"Keslova-Veselikova J, Hulkova H, Dobrovolny R, Asfaw B, Poupetova H, Berna L, Sikora J, Golan L, Ledvinova J, Elleder M (2008) Replacement of alpha-galactosidase A in Fabry disease: effect on fibroblast cultures compared with biopsied tissues of treated patients. Virchows Arch 452:651\u2013665. https:\/\/doi.org\/10.1007\/s00428-008-0586-9","journal-title":"Virchows Arch"},{"key":"452_CR10","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/j.ymgme.2014.08.002","volume":"114","author":"T Kizhner","year":"2015","unstructured":"Kizhner T, Azulay Y, Hainrichson M, Tekoah Y, Arvatz G, Shulman A, Ruderfer I, Aviezer D, Shaaltiel Y (2015) Characterization of a chemically modified plant cell culture expressed human alpha-galactosidase-A enzyme for treatment of Fabry disease. Mol Genet Metab 114:259\u2013267. https:\/\/doi.org\/10.1016\/j.ymgme.2014.08.002","journal-title":"Mol Genet Metab"},{"key":"452_CR11","doi-asserted-by":"publisher","first-page":"1018","DOI":"10.1056\/NEJMoa2002699","volume":"383","author":"BA Konkle","year":"2020","unstructured":"Konkle BA, Shapiro AD, Quon DV, Staber JM, Kulkarni R, Ragni MV, Chhabra ES, Poloskey S, Rice K, Katragadda S et al (2020) BIVV001 fusion protein as factor VIII replacement therapy for hemophilia A. N Engl J Med 383:1018\u20131027. https:\/\/doi.org\/10.1056\/NEJMoa2002699","journal-title":"N Engl J Med"},{"key":"452_CR12","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1007\/s40265-021-01486-1","volume":"81","author":"M Lenders","year":"2021","unstructured":"Lenders M, Brand E (2021) Fabry disease: the current treatment landscape. Drugs 81:635\u2013645. https:\/\/doi.org\/10.1007\/s40265-021-01486-1","journal-title":"Drugs"},{"key":"452_CR13","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1007\/s11248-012-9623-1","volume":"22","author":"H Li","year":"2013","unstructured":"Li H, Liu Q, Cui K, Liu J, Ren Y, Shi D (2013) Expression of biologically active human interferon alpha 2b in the milk of transgenic mice. Transgenic Res 22:169\u2013178. https:\/\/doi.org\/10.1007\/s11248-012-9623-1","journal-title":"Transgenic Res"},{"key":"452_CR14","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1016\/s0167-5699(00)01668-6","volume":"21","author":"M Little","year":"2000","unstructured":"Little M, Kipriyanov SM, Le Gall F, Moldenhauer G (2000) Of mice and men: hybridoma and recombinant antibodies. Immunol Today 21:364\u2013370. https:\/\/doi.org\/10.1016\/s0167-5699(00)01668-6","journal-title":"Immunol Today"},{"key":"452_CR15","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1038\/nrd4363","volume":"13","author":"S Mitragotri","year":"2014","unstructured":"Mitragotri S, Burke PA, Langer R (2014) Overcoming the challenges in administering biopharmaceuticals: formulation and delivery strategies. Nat Rev Drug Discov 13:655\u2013672. https:\/\/doi.org\/10.1038\/nrd4363","journal-title":"Nat Rev Drug Discov"},{"key":"452_CR16","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1080\/21655979.2016.1171429","volume":"7","author":"PS Monzani","year":"2016","unstructured":"Monzani PS, Adona PR, Ohashi OM, Meirelles FV, Wheeler MB (2016) Transgenic bovine as bioreactors: challenges and perspectives. Bioengineered 7:123\u2013131. https:\/\/doi.org\/10.1080\/21655979.2016.1171429","journal-title":"Bioengineered"},{"key":"452_CR17","doi-asserted-by":"publisher","first-page":"91","DOI":"10.20452\/pamw.15117","volume":"130","author":"M Nowicki","year":"2020","unstructured":"Nowicki M, Bazan-Socha S, Blazejewska-Hyzorek B, Gellert R, Imiela J, Kazmierczak J, Klopotowski M, Oko-Sarnowska Z, Pawlaczyk K, Ponikowski P et al (2020) Enzyme replacement therapy in Fabry disease in Poland: a position statement. Pol Arch Intern Med 130:91\u201397. https:\/\/doi.org\/10.20452\/pamw.15117","journal-title":"Pol Arch Intern Med"},{"key":"452_CR18","doi-asserted-by":"publisher","first-page":"1630","DOI":"10.1021\/acs.bioconjchem.8b00133","volume":"29","author":"I Ruderfer","year":"2018","unstructured":"Ruderfer I, Shulman A, Kizhner T, Azulay Y, Nataf Y, Tekoah Y, Shaaltiel Y (2018) Development and Analytical Characterization of pegunigalsidase alfa, a chemically cross-linked plant recombinant human alpha-galactosidase-A for Treatment of Fabry disease. Bioconjug Chem 29:1630\u20131639. https:\/\/doi.org\/10.1021\/acs.bioconjchem.8b00133","journal-title":"Bioconjug Chem"},{"key":"452_CR19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgmr.2019.100562","volume":"22","author":"T Sawada","year":"2020","unstructured":"Sawada T, Kido J, Yoshida S, Sugawara K, Momosaki K, Inoue T, Tajima G, Sawada H, Mastumoto S, Endo F et al (2020) Newborn screening for Fabry disease in the western region of Japan. Mol Genet Metab Rep 22:100562. https:\/\/doi.org\/10.1016\/j.ymgmr.2019.100562","journal-title":"Mol Genet Metab Rep"},{"key":"452_CR20","doi-asserted-by":"publisher","first-page":"1186","DOI":"10.1038\/nbt.1588","volume":"27","author":"V Schellenberger","year":"2009","unstructured":"Schellenberger V, Wang CW, Geething NC, Spink BJ, Campbell A, To W, Scholle MD, Yin Y, Yao Y, Bogin O et al (2009) A recombinant polypeptide extends the in vivo half-life of peptides and proteins in a tunable manner. Nat Biotechnol 27:1186\u20131190. https:\/\/doi.org\/10.1038\/nbt.1588","journal-title":"Nat Biotechnol"},{"key":"452_CR22","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1016\/j.kint.2016.10.004","volume":"91","author":"R Schiffmann","year":"2017","unstructured":"Schiffmann R, Hughes DA, Linthorst GE, Ortiz A, Svarstad E, Warnock DG, West ML, Wanner C, Conference Participants (2017) Screening, diagnosis, and management of patients with Fabry disease: conclusions from a \u201cKidney Disease: Improving Global Outcomes\u201d (KDIGO) Controversies Conference. Kidney Int 91:284\u2013293. https:\/\/doi.org\/10.1016\/j.kint.2016.10.004","journal-title":"Kidney Int"},{"key":"452_CR21","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1002\/jimd.12080","volume":"42","author":"R Schiffmann","year":"2019","unstructured":"Schiffmann R, Goker-Alpan O, Holida M, Giraldo P, Barisoni L, Colvin RB, Jennette CJ, Maegawa G, Boyadjiev SA, Gonzalez D et al (2019) Pegunigalsidase alfa, a novel PEGylated enzyme replacement therapy for Fabry disease, provides sustained plasma concentrations and favorable pharmacodynamics: a 1-year phase 1\/2 clinical trial. J Inherit Metab Dis 42:534\u2013544. https:\/\/doi.org\/10.1002\/jimd.12080","journal-title":"J Inherit Metab Dis"},{"key":"452_CR23","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1111\/jpn.12039","volume":"98","author":"T Sigl","year":"2014","unstructured":"Sigl T, Meyer HH, Wiedemann S (2014) Gene expression analysis of protein synthesis pathways in bovine mammary epithelial cells purified from milk during lactation and short-term restricted feeding. J Anim Physiol Anim Nutr 98:84\u201395. https:\/\/doi.org\/10.1111\/jpn.12039","journal-title":"J Anim Physiol Anim Nutr"},{"key":"452_CR24","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1590\/2175-8239-JBN-2021-0208","volume":"44","author":"CAB Silva","year":"2022","unstructured":"Silva CAB, Andrade LGM, Vaisbich MH, Barreto FC (2022) Brazilian consensus recommendations for the diagnosis, screening, and treatment of individuals with fabry disease: Committee for Rare Diseases\u2014Brazilian Society of Nephrology\/2021. J Bras Nefrol 44:249\u2013267. https:\/\/doi.org\/10.1590\/2175-8239-JBN-2021-0208","journal-title":"J Bras Nefrol"},{"key":"452_CR25","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/s13554-012-0003-4","volume":"2","author":"R Sophie","year":"2012","unstructured":"Sophie R, Akhtar A, Sepah YJ, Ibrahim M, Bittencourt M, Do DV, Nguyen QD (2012) Aflibercept: a potent vascular endothelial growth factor antagonist for neovascular age-related macular degeneration and other retinal vascular diseases. Biol Ther 2:3. https:\/\/doi.org\/10.1007\/s13554-012-0003-4","journal-title":"Biol Ther"},{"issue":"Pt 1","key":"452_CR26","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1042\/bj3390033","volume":"339","author":"MG Stinnakre","year":"1999","unstructured":"Stinnakre MG, Soulier S, Schibler L, Lepourry L, Mercier JC, Vilotte JL (1999) Position-independent and copy-number-related expression of a goat bacterial artificial chromosome alpha-lactalbumin gene in transgenic mice. Biochem J 339(Pt 1):33\u201336","journal-title":"Biochem J"},{"key":"452_CR27","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1007\/s11248-007-9162-3","volume":"17","author":"B Tang","year":"2008","unstructured":"Tang B, Yu S, Zheng M, Ding F, Zhao R, Zhao J, Dai Y, Li N (2008) High level expression of a functional human\/mouse chimeric anti-CD20 monoclonal antibody in milk of transgenic mice. Transgenic Res 17:727\u2013732. https:\/\/doi.org\/10.1007\/s11248-007-9162-3","journal-title":"Transgenic Res"},{"key":"452_CR28","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/580463","volume":"2013","author":"Y Wang","year":"2013","unstructured":"Wang Y, Zhao S, Bai L, Fan J, Liu E (2013) Expression systems and species used for transgenic animal bioreactors. Biomed Res Int 2013:580463. https:\/\/doi.org\/10.1155\/2013\/580463","journal-title":"Biomed Res Int"},{"key":"452_CR29","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.ymgme.2018.06.004","volume":"124","author":"C Wanner","year":"2018","unstructured":"Wanner C, Arad M, Baron R, Burlina A, Elliott PM, Feldt-Rasmussen U, Fomin VV, Germain DP, Hughes DA, Jovanovic A et al (2018) European expert consensus statement on therapeutic goals in Fabry disease. Mol Genet Metab 124:189\u2013203. https:\/\/doi.org\/10.1016\/j.ymgme.2018.06.004","journal-title":"Mol Genet Metab"},{"key":"452_CR30","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1007\/s10529-020-02808-1","volume":"42","author":"H Yan","year":"2020","unstructured":"Yan H, Gong X, Xu M, Guo X, Chen Y, Xue Y, Zeng Y, Zeng F (2020) Production of biologically active human factor IX-Fc fusion protein in the milk of transgenic mice. Biotechnol Lett 42:717\u2013726. https:\/\/doi.org\/10.1007\/s10529-020-02808-1","journal-title":"Biotechnol Lett"},{"key":"452_CR31","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/s0093-691x(97)00408-1","volume":"49","author":"CA Ziomek","year":"1998","unstructured":"Ziomek CA (1998) Commercialization of proteins produced in the mammary gland. Theriogenology 49:139\u2013144. https:\/\/doi.org\/10.1016\/s0093-691x(97)00408-1","journal-title":"Theriogenology"}],"container-title":["Transgenic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11248-025-00452-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11248-025-00452-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11248-025-00452-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T10:35:29Z","timestamp":1751452529000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11248-025-00452-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,2]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["452"],"URL":"https:\/\/doi.org\/10.1007\/s11248-025-00452-x","relation":{},"ISSN":["0962-8819","1573-9368"],"issn-type":[{"value":"0962-8819","type":"print"},{"value":"1573-9368","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,2]]},"assertion":[{"value":"26 January 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This study was carried out in strict accordance with the guidelines for the care and use of animals of Chengdu Institute of Biological Products Co., Ltd. All animal experimental procedures were approved by the Animal Care Commission of Chengdu Institute of Biological Products Co., Ltd. Every effort was made to minimize animal pain, suffering, and distress and to reduce the number of animals used.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}],"article-number":"33"}}