{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T10:43:16Z","timestamp":1778409796729,"version":"3.51.4"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Department of Science and Technology, Govt of India","award":["SR\/WOS-A\/LS-405\/2018"],"award-info":[{"award-number":["SR\/WOS-A\/LS-405\/2018"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Transgenic Res"],"DOI":"10.1007\/s11248-026-00485-w","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:36:15Z","timestamp":1772170575000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Targeted expression of Glycine max isoflavone synthase enhances daidzein and genistein content in soybean"],"prefix":"10.1007","volume":"35","author":[{"given":"Nandakumar","family":"Vidya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishnagowdu","family":"Saravanan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jayachandran","family":"Halka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumaresan","family":"Kowsalya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Packiaraj","family":"Gurusaravanan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muthukrishnan","family":"Anand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gomathi","family":"Selvavinayagam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesudass Joseph","family":"Sahayarayan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muthukrishnan","family":"Arun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"key":"485_CR1","doi-asserted-by":"publisher","first-page":"636","DOI":"10.4161\/psb.20039","volume":"7","author":"K Abdel-Lateif","year":"2012","unstructured":"Abdel-Lateif K, Bogusz D, Hocher V (2012) The role of flavonoids in the establishment of plant roots endosymbioses with arbuscular mycorrhiza fungi, rhizobia and Frankia bacteria. Plant Signal Behav 7:636\u2013641. https:\/\/doi.org\/10.4161\/psb.20039","journal-title":"Plant Signal Behav"},{"key":"485_CR2","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1104\/pp.121.3.821","volume":"121","author":"T Akashi","year":"1999","unstructured":"Akashi T, Aoki T, Ayabe S (1999) Cloning and functional expression of a cytochrome P450 cDNA encoding 2-hydroxyisoflavanone synthase involved in biosynthesis of the isoflavonoid skeleton in licorice. Plant Physiol 121:821\u2013828. https:\/\/doi.org\/10.1104\/pp.121.3.821","journal-title":"Plant Physiol"},{"key":"485_CR3","doi-asserted-by":"publisher","first-page":"2266","DOI":"10.1007\/s12010-014-1360-x","volume":"175","author":"M Arun","year":"2015","unstructured":"Arun M, Subramanyam K, Mariashibu TS et al (2015) Application of sonication in combination with vacuum infiltration enhances the Agrobacterium-mediated genetic transformation in Indian soybean cultivars. Appl Biochem Biotechnol 175:2266\u20132287. https:\/\/doi.org\/10.1007\/s12010-014-1360-x","journal-title":"Appl Biochem Biotechnol"},{"key":"485_CR4","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-018-1153-y","volume":"8","author":"M Balasubramanian","year":"2018","unstructured":"Balasubramanian M, Anbumegala M, Surendran R et al (2018) Elite hairy roots of Raphanus sativus (L.) as a source of antioxidants and flavonoids. 3 Biotech 8:128. https:\/\/doi.org\/10.1007\/s13205-018-1153-y","journal-title":"3 Biotech"},{"key":"485_CR5","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11164676","volume":"11","author":"A Bara\u0144ska","year":"2022","unstructured":"Bara\u0144ska A, Kanadys W, Bogdan M et al (2022) The role of soy isoflavones in the prevention of bone loss in postmenopausal women: a systematic review with meta-analysis of randomized controlled trials. J Clin Med 11:4676. https:\/\/doi.org\/10.3390\/jcm11164676","journal-title":"J Clin Med"},{"key":"485_CR6","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1006\/abio.1996.0292","volume":"239","author":"IFF Benzie","year":"1996","unstructured":"Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of \u201cantioxidant power\u201d: the FRAP assay. Anal Biochem 239:70\u201376. https:\/\/doi.org\/10.1006\/abio.1996.0292","journal-title":"Anal Biochem"},{"key":"485_CR7","doi-asserted-by":"publisher","first-page":"1199","DOI":"10.1038\/1811199a0","volume":"181","author":"MS Blois","year":"1958","unstructured":"Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199\u20131200. https:\/\/doi.org\/10.1038\/1811199a0","journal-title":"Nature"},{"key":"485_CR8","doi-asserted-by":"publisher","first-page":"2245","DOI":"10.1104\/pp.105.062539","volume":"138","author":"BE Deavours","year":"2005","unstructured":"Deavours BE, Dixon RA (2005) Metabolic engineering of isoflavonoid biosynthesis in alfalfa. Plant Physiol 138:2245\u20132259. https:\/\/doi.org\/10.1104\/pp.105.062539","journal-title":"Plant Physiol"},{"key":"485_CR9","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-020-2065-1","volume":"10","author":"MKA Devi","year":"2020","unstructured":"Devi MKA, Kumar G, Giridhar P (2020) Effect of biotic and abiotic elicitors on isoflavone biosynthesis during seed development and in suspension cultures of soybean (Glycine max L.). 3 Biotech 10:98. https:\/\/doi.org\/10.1007\/s13205-020-2065-1","journal-title":"3 Biotech"},{"key":"485_CR10","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1021\/jf00116a052","volume":"31","author":"A Eldridge","year":"1983","unstructured":"Eldridge A, Kwolek W (1983) Soybean isoflavones: effect of environment and variety on composition. J Agric Food Chem 31:394\u2013396","journal-title":"J Agric Food Chem"},{"key":"485_CR11","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1111\/pbi.12445","volume":"14","author":"L Gou","year":"2016","unstructured":"Gou L, Zhang R, Ma L et al (2016) Multigene synergism increases the isoflavone and proanthocyanidin contents of Medicago truncatula. Plant Biotechnol J 14:915\u2013925. https:\/\/doi.org\/10.1111\/pbi.12445","journal-title":"Plant Biotechnol J"},{"key":"485_CR12","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/S1360-1385(00)01680-0","volume":"5","author":"JM Humphreys","year":"2000","unstructured":"Humphreys JM, Chapple C (2000) Molecular \u2018pharming\u2019 with plant P450s. Trends Plant Sci 5:271\u2013272. https:\/\/doi.org\/10.1016\/S1360-1385(00)01680-0","journal-title":"Trends Plant Sci"},{"key":"485_CR13","doi-asserted-by":"publisher","first-page":"3901","DOI":"10.1002\/j.1460-2075.1987.tb02730.x","volume":"6","author":"RA Jefferson","year":"1987","unstructured":"Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901\u20133907. https:\/\/doi.org\/10.1002\/j.1460-2075.1987.tb02730.x","journal-title":"EMBO J"},{"key":"485_CR14","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1007\/s11816-010-0143-2","volume":"4","author":"N Jiang","year":"2010","unstructured":"Jiang N, Jeon E-H, Pak J-H et al (2010) Increase of isoflavones in soybean callus by Agrobacterium-mediated transformation. Plant Biotechnol Rep 4:253\u2013260. https:\/\/doi.org\/10.1007\/s11816-010-0143-2","journal-title":"Plant Biotechnol Rep"},{"key":"485_CR15","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1038\/72671","volume":"18","author":"W Jung","year":"2000","unstructured":"Jung W, Yu O, Lau S et al (2000) Identification and expression of isoflavone synthase, the key enzyme for biosynthesis of isoflavones in legumes. Nat Biotechnol 18:208\u2013212","journal-title":"Nat Biotechnol"},{"key":"485_CR16","doi-asserted-by":"publisher","first-page":"2935","DOI":"10.1039\/C7FO00342K","volume":"8","author":"Y Li","year":"2017","unstructured":"Li Y, Zhang H (2017) Soybean isoflavones ameliorate ischemic cardiomyopathy by activating Nrf2-mediated antioxidant responses. Food Funct 8:2935\u20132944. https:\/\/doi.org\/10.1039\/C7FO00342K","journal-title":"Food Funct"},{"key":"485_CR17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ymben.2006.08.003","volume":"9","author":"R Liu","year":"2007","unstructured":"Liu R, Hu Y, Li J, Lin Z (2007) Production of soybean isoflavone genistein in non-legume plants via genetically modified secondary metabolism pathway. Metab Eng 9:1\u20137. https:\/\/doi.org\/10.1016\/j.ymben.2006.08.003","journal-title":"Metab Eng"},{"key":"485_CR18","volume":"20","author":"E Lopez","year":"2015","unstructured":"Lopez E, Proano K, Jadan M, Mihai R (2015) Callus tissue induction and analysis of GUS reporter gene expression in tomato (Solanum lycopersicum L.) transformed with Agrobacterium tumefaciens. Rom Biotechnol Lett 20:10205","journal-title":"Rom Biotechnol Lett"},{"key":"485_CR19","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.jsbmb.2013.12.010","volume":"140","author":"AM Mahmoud","year":"2014","unstructured":"Mahmoud AM, Yang W, Bosland MC (2014) Soy isoflavones and prostate cancer: a review of molecular mechanisms. J Steroid Biochem Mol Biol 140:116\u2013132. https:\/\/doi.org\/10.1016\/j.jsbmb.2013.12.010","journal-title":"J Steroid Biochem Mol Biol"},{"key":"485_CR20","doi-asserted-by":"publisher","DOI":"10.3390\/plants10112311","volume":"10","author":"A Malla","year":"2021","unstructured":"Malla A, Shanmugaraj B, Sharma A, Ramalingam S (2021) Production of genistein in Amaranthus tricolor var. tristis and Spinacia oleracea by expression of Glycine max isoflavone synthase. Plants 10:2311. https:\/\/doi.org\/10.3390\/plants10112311","journal-title":"Plants"},{"key":"485_CR21","doi-asserted-by":"publisher","DOI":"10.3390\/plants10010052","volume":"10","author":"A Malla","year":"2020","unstructured":"Malla A, Shanmugaraj B, Srinivasan B et al (2020) Metabolic engineering of isoflavonoid biosynthesis by expressing Glycine max isoflavone synthase in Allium cepa L. for genistein production. Plants 10:52. https:\/\/doi.org\/10.3390\/plants10010052","journal-title":"Plants"},{"key":"485_CR22","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1007\/s11240-023-02577-5","volume":"155","author":"S Nayeem","year":"2023","unstructured":"Nayeem S, Sundararajan S, Rajendran V et al (2023) Metabolic engineering of isoflavonoid genistein in indica rice by expressing Isoflavone Synthase from Glycine max. Plant Cell, Tissue and Organ Culture (PCTOC) 155:243\u2013253. https:\/\/doi.org\/10.1007\/s11240-023-02577-5","journal-title":"Plant Cell, Tissue and Organ Culture (PCTOC)"},{"issue":"6","key":"485_CR23","doi-asserted-by":"publisher","first-page":"842","DOI":"10.1007\/s11627-020-10076-x","volume":"56","author":"HQ Nguyen","year":"2020","unstructured":"Nguyen HQ, Hong T, Le T et al (2020) Overexpressing GmCHI1A increases the isoflavone content of transgenic soybean (Glycine max ( L.) Merr.) seeds. Vitr Cell Dev Biol-Plant. 56(6):842. https:\/\/doi.org\/10.1007\/s11627-020-10076-x","journal-title":"Vitr Cell Dev Biol-Plant."},{"key":"485_CR24","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27248899","volume":"27","author":"T Ohishi","year":"2022","unstructured":"Ohishi T, Miyoshi N, Mori M et al (2022) Health effects of soy isoflavones and green tea catechins on cancer and cardiovascular diseases based on urinary biomarker levels. Molecules 27:8899. https:\/\/doi.org\/10.3390\/molecules27248899","journal-title":"Molecules"},{"key":"485_CR25","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01035","author":"S Ratjens","year":"2018","unstructured":"Ratjens S, Mortensen S, Kumpf A et al (2018) Embryogenic callus as target for efficient transformation of Cyclamen persicum enabling gene function studies. Front Plant Sci. https:\/\/doi.org\/10.3389\/fpls.2018.01035","journal-title":"Front Plant Sci"},{"key":"485_CR26","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-023-03715-8","volume":"13","author":"K Saravanan","year":"2023","unstructured":"Saravanan K, Vidya N, Appunu C et al (2023) A simple and efficient genetic transformation system for soybean (Glycine max (L.) Merrill) targeting apical meristem of modified half-seed explant. 3 Biotech 13:293. https:\/\/doi.org\/10.1007\/s13205-023-03715-8","journal-title":"3 Biotech"},{"key":"485_CR27","first-page":"149","volume":"113\u2013114","author":"H Satoh","year":"2003","unstructured":"Satoh H, Nishikawa K, Suzuki K, Asano R, Virgona N, Ichikawa T, Hagiwara KYT (2003) Genistein, a soy isoflavone, enhances necrotic-like cell death in a breast cancer cell treated with a chemotherapeutic agent. Res Commun Mol Pathol Pharmacol 113\u2013114:149\u2013158","journal-title":"Res Commun Mol Pathol Pharmacol"},{"key":"485_CR28","doi-asserted-by":"publisher","first-page":"175","DOI":"10.31924\/nrsd.v10i2.053","volume":"10","author":"P Shrestha","year":"2020","unstructured":"Shrestha P, Modi B, Aryal Surya P et al (2020) Total phenolic and flavonoids contents, antioxidant activities, phytochemical and nutritional analysis of Castanopsis indica (Indian Chestnut). Nat Res Sustain Dev 10:175\u2013187. https:\/\/doi.org\/10.31924\/nrsd.v10i2.053","journal-title":"Nat Res Sustain Dev"},{"key":"485_CR29","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1007\/s11816-016-0385-8","volume":"10","author":"G Sivanandhan","year":"2016","unstructured":"Sivanandhan G, Arunachalam C, Vasudevan V et al (2016) Factors affecting Agrobacterium-mediated transformation in Hybanthus enneaspermus (L.) F. Muell. Plant Biotechnol Rep 10:49\u201360. https:\/\/doi.org\/10.1007\/s11816-016-0385-8","journal-title":"Plant Biotechnol Rep"},{"key":"485_CR30","doi-asserted-by":"publisher","first-page":"49","DOI":"10.5344\/ajev.1977.28.1.49","volume":"28","author":"K Slinkard","year":"1977","unstructured":"Slinkard K, Singleton VL (1977) Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic 28:49\u201355. https:\/\/doi.org\/10.5344\/ajev.1977.28.1.49","journal-title":"Am J Enol Vitic"},{"key":"485_CR31","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1111\/j.1365-313X.2006.02874.x","volume":"48","author":"S Subramanian","year":"2006","unstructured":"Subramanian S, Stacey G, Yu O (2006) Endogenous isoflavones are essential for the establishment of symbiosis between soybean and Bradyrhizobium japonicum. Plant J 48:261\u2013273. https:\/\/doi.org\/10.1111\/j.1365-313X.2006.02874.x","journal-title":"Plant J"},{"key":"485_CR32","doi-asserted-by":"publisher","first-page":"966","DOI":"10.1111\/tpj.14083","volume":"96","author":"A Sukumaran","year":"2018","unstructured":"Sukumaran A, McDowell T, Chen L et al (2018) Isoflavonoid-specific prenyltransferase gene family in soybean: GmPT01, a pterocarpan 2-dimethylallyltransferase involved in glyceollin biosynthesis. Plant J 96:966\u2013981. https:\/\/doi.org\/10.1111\/tpj.14083","journal-title":"Plant J"},{"key":"485_CR33","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1007\/s10142-010-0197-9","volume":"11","author":"X Wang","year":"2011","unstructured":"Wang X (2011) Structure, function, and engineering of enzymes in isoflavonoid biosynthesis. Funct Integr Genomics 11:13\u201322. https:\/\/doi.org\/10.1007\/s10142-010-0197-9","journal-title":"Funct Integr Genomics"},{"issue":"2","key":"485_CR34","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1104\/pp.124.2.781","volume":"124","author":"O Yu","year":"2000","unstructured":"Yu O, Jung W, Shi J et al (2000) Production of the isoflavones genistein and daidzein in non-legume dicot and monocot tissues. Plant Physiol 124(2):781","journal-title":"Plant Physiol"},{"key":"485_CR35","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1104\/pp.124.2.781","volume":"124","author":"O Yu","year":"2000","unstructured":"Yu O, Jung W, Shi J et al (2000b) Production of the isoflavones genistein and daidzein in non-legume dicot and monocot tissues. Plant Physiol 124:781\u2013794. https:\/\/doi.org\/10.1104\/pp.124.2.781","journal-title":"Plant Physiol"},{"key":"485_CR36","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1016\/S0031-9422(03)00345-5","volume":"63","author":"O Yu","year":"2003","unstructured":"Yu O, Shi J, Hession AO et al (2003) Metabolic engineering to increase isoflavone biosynthesis in soybean seed. Phytochemistry 63:753\u2013763. https:\/\/doi.org\/10.1016\/S0031-9422(03)00345-5","journal-title":"Phytochemistry"},{"key":"485_CR37","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1016\/S0308-8146(98)00102-2","volume":"64","author":"J Zhishen","year":"1999","unstructured":"Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555\u2013559. https:\/\/doi.org\/10.1016\/S0308-8146(98)00102-2","journal-title":"Food Chem"}],"container-title":["Transgenic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11248-026-00485-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11248-026-00485-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11248-026-00485-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:36:16Z","timestamp":1772170576000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11248-026-00485-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,27]]},"references-count":37,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["485"],"URL":"https:\/\/doi.org\/10.1007\/s11248-026-00485-w","relation":{},"ISSN":["1573-9368"],"issn-type":[{"value":"1573-9368","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,27]]},"assertion":[{"value":"22 April 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 February 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 February 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"10"}}