{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:02:23Z","timestamp":1762506143252},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2013,7,19]],"date-time":"2013-07-19T00:00:00Z","timestamp":1374192000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Trop Anim Health Prod"],"published-print":{"date-parts":[[2014,1]]},"DOI":"10.1007\/s11250-013-0448-6","type":"journal-article","created":{"date-parts":[[2013,7,18]],"date-time":"2013-07-18T14:28:17Z","timestamp":1374157697000},"page":"73-78","source":"Crossref","is-referenced-by-count":25,"title":["Isolation and identification of bovine Brucella isolates from Pakistan by biochemical tests and PCR"],"prefix":"10.1007","volume":"46","author":[{"given":"Shahzad","family":"Ali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qurban","family":"Ali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Falk","family":"Melzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iahtasham","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shamim","family":"Akhter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinrich","family":"Neubauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syed M.","family":"Jamal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,7,19]]},"reference":[{"key":"448_CR1","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/j.1865-1682.2010.01171.x","volume":"57","author":"M Abubakar","year":"2010","unstructured":"Abubakar, M., Javed-Arshed, M., Hussain, M., Ehtisham, H., Ali, Q., 2010. Serological evidence of Brucella abortus prevalence in Punjab province, Pakistan\u2014A cross-sectional study. Transboundary and Emerging Diseases, 57, 443\u2013447.","journal-title":"Transboundary and Emerging Diseases"},{"key":"448_CR2","first-page":"147","volume":"32","author":"M Abubakar","year":"2012","unstructured":"Abubakar, M., Mansoor, M., Hussain, M., Javed-Arshed, M., 2012. Bovine brucellosis: old and new concepts with Pakistan perspective. Pakistan Veterinary Journal, 32, 147\u2013155.","journal-title":"Pakistan Veterinary Journal"},{"key":"448_CR3","first-page":"391","volume":"81","author":"AA Adesiyun","year":"2011","unstructured":"Adesiyun, A.A., Baird, K., Stewart-Johnson, A., 2011. Antimicrobial resistance, phenotypic characteristics, phage types of Brucella abortus strains isolated from cattle and water buffalo (Bubalis bubalis) in Trinidad. Veterinary Archive, 81, 391\u2013404.","journal-title":"Veterinary Archive"},{"key":"448_CR4","first-page":"33","volume":"1","author":"YF Ahmed","year":"2010","unstructured":"Ahmed, Y.F., Sokkar, S.M., Desouky, H.M., Ghazi, Y.A., Amin, A.S., Madboly, A.A., 2010. Pathological and molecular studies on mammary glands and supramammary lymph node of naturally Brucella infected buffalo-cows. Journal of Reproduction and Infertility, 1, 33\u201340.","journal-title":"Journal of Reproduction and Infertility"},{"key":"448_CR5","first-page":"13","volume-title":"Techniques for the Brucellosis Laboratory","author":"GG Alton","year":"1988","unstructured":"Alton, G.G., Jones, L.M., Angus, R.D., Verger, J.M., 1988. Bacteriological methods. In: G.G. Alton, L.M. Jones, R.D. Angus and J.M. Verger (eds), Techniques for the Brucellosis Laboratory. Paris: Institut National De La Recherche Agronomique, pp 13\u201361."},{"key":"448_CR6","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1128\/JCM.32.11.2660-2666.1994","volume":"32","author":"BJ Bricker","year":"1994","unstructured":"Bricker, B.J., Halling, S.M., 1994. Differentiation of Brucella abortus bv. 1, 2 and 4, Brucella melitensis, Brucella ovis and Brucella suis bv. 1 by PCR. Journal of Clinical Microbiology, 32, 2660\u20132666.","journal-title":"Journal of Clinical Microbiology"},{"key":"448_CR7","first-page":"109","volume":"14","author":"A Doosti","year":"2011","unstructured":"Doosti, A., Dehkordi, P.G., 2011. Application of real-time PCR for identification and differentiation of Brucella abortus and Brucella melitensis in cattle. Bulgarian Journal of Veterinary Medicine, 14, 109\u2013115.","journal-title":"Bulgarian Journal of Veterinary Medicine"},{"key":"448_CR8","unstructured":"Economic Survey, 2011\u20132012. Economic Survey of Pakistan. Ministry of Finance, Government of Pakistan, Islamabad, pp 15\u221233."},{"key":"448_CR9","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1111\/j.1365-2672.1972.tb03744.x","volume":"35","author":"ID Farrell","year":"1972","unstructured":"Farrell, I.D., Robinson, L., 1972. A comparison of various selective media, including a new selective medium for the isolation of brucellae from milk. Journal of Applied Bacteriology, 35, 625\u2013630.","journal-title":"Journal of Applied Bacteriology"},{"key":"448_CR10","first-page":"242","volume":"61","author":"M Hamidullah","year":"2009","unstructured":"Hamidullah, M., Khan, R., Khan, I., 2009. Seroprevalence of brucellosis in animals in district Kohat NWFP and comparison of two serological tests. Pakistan Journal of Science, 61, 242\u2013243.","journal-title":"Pakistan Journal of Science"},{"key":"448_CR11","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.ijmm.2009.05.002","volume":"299","author":"B Huber","year":"2009","unstructured":"Huber, B., Scholz, H.C., Lucero, N., Busse, H.J., 2009. Development of a PCR assay for typing and subtyping of Brucella species. International Journal of Medical Microbiology, 299, 563\u2013573.","journal-title":"International Journal of Medical Microbiology"},{"key":"448_CR12","unstructured":"Kanani, A.N., 2007. Serological, cultural and molecular detection of Brucella infection in breeding bulls. PhD thesis, Anand Agricultural University, India."},{"key":"448_CR13","first-page":"41","volume":"1","author":"SS Kumar","year":"2004","unstructured":"Kumar, S.S., Basanti, B., 2004. Isolation and identification of Brucella abortus from cattle of Gaushal and its antibiotics sensitivity. Indian Cow Journal, 1, 41\u201344.","journal-title":"Indian Cow Journal"},{"key":"448_CR14","first-page":"4036","volume":"6","author":"DI Martinez-Herrera","year":"2012","unstructured":"Martinez-Herrera, D.I., Pulido-Camarillo, E., Pardio-Sedas, V.T., Lopez-Merino, A., del Carmen Sarabia-Bueno, C., Loeza-Limon, R., Morales-Alvarez, J.F., Flores-Castro, R., 2012. Isolation of Brucella abortus from the milk of serum positive cows using chicken embryos as amplifier. African Journal of Microbiology Research, 6, 4036\u20134040.","journal-title":"African Journal of Microbiology Research"},{"key":"448_CR15","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s11250-011-9899-9","volume":"44","author":"EN Muendo","year":"2012","unstructured":"Muendo, E.N., Mbatha, P.M., Macharia, J., Abdoel, T.H., Janszen, P.V., Pastoor, R., Smits, H.L., 2012. Infection of cattle in Kenya with Brucella abortus biovar 3 and Brucella melitensis biovar 1 genotypes. Tropical Animal Health and Production, 44, 17\u201320.","journal-title":"Tropical Animal Health and Production"},{"key":"448_CR16","first-page":"1031","volume":"10","author":"F Mukhtar","year":"2010","unstructured":"Mukhtar, F., 2010. Brucellosis in a high risk occupational group: seroprevalence and analysis of risk factors. Journal of the Pakistan Medical Association, 10, 1031\u20131034.","journal-title":"Journal of the Pakistan Medical Association"},{"key":"448_CR17","first-page":"35","volume":"2","author":"R Munir","year":"2011","unstructured":"Munir, R., Umer, F., Zahida, F., Muhammad, A., Zubair, A., Muhammad, J., 2011. Sero-prevalence of brucellosis in bovine at farms under different management conditions. British Journal of Dairy Science, 2, 35\u201339.","journal-title":"British Journal of Dairy Science"},{"key":"448_CR18","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s11250-011-9886-1","volume":"44","author":"M Nagalingam","year":"2012","unstructured":"Nagalingam, M., Shome, R., Balamurugan, V., Bibek, R., Shome, B.R., Rao, K.R., Vivekananda, I.S., Prabhudas, K., 2012. Molecular typing of Brucella species isolates from livestock and human. Tropical Animal Health Production, 44, 5\u20139.","journal-title":"Tropical Animal Health Production"},{"key":"448_CR19","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.vetmic.2004.06.012","volume":"103","author":"RA Ocholi","year":"2004","unstructured":"Ocholi, R.A., Kwaga, J.K.P., Ajogi, I., Bale, J.O.O., 2004. Phenotypic characterization of Brucella strains isolated from livestock in Nigeria. Veterinary Microbiology, 103, 47\u201353.","journal-title":"Veterinary Microbiology"},{"key":"448_CR20","doi-asserted-by":"crossref","first-page":"542","DOI":"10.5455\/vetworld.2011.542-545","volume":"4","author":"MAR Priyantha","year":"2011","unstructured":"Priyantha, M.A.R., 2011. Identification of biovars of Brucella abortus in aborted cattle and buffaloes herd in Sri Lanka. Veterinary World, 4, 542\u2013545.","journal-title":"Veterinary World"},{"key":"448_CR21","author":"M Shafee","year":"2011","unstructured":"Shafee, M., Masood, R., Ali, A.S., Mansoor, D.A., Abdul, R., 2011. Prevalence of bovine brucellosis in organized dairy farms, using milk ELISA, in Quetta City, Balochistan, Pakistan. Veterinary Medicine International, doi: 10.4061\/2011\/358950 .","journal-title":"Veterinary Medicine International"},{"key":"448_CR22","first-page":"116","volume":"4","author":"O Shahaza","year":"2009","unstructured":"Shahaza, O., Khairani-Bejo, S., Zunita, Z., Bahaman, A.R., 2009. In-House Rose Bengal Plate agglutination Test (RBPT) for a rapid diagnosis of brucellosis in goats in Malaysia. International Journal of Tropical Medicine, 4, 116\u2013118.","journal-title":"International Journal of Tropical Medicine"},{"key":"448_CR23","doi-asserted-by":"crossref","first-page":"19","DOI":"10.33899\/ijvs.2006.62486","volume":"20","author":"JM Shareef","year":"2006","unstructured":"Shareef, J.M., 2006. Isolation of Brucella abortus from aborted lambs in the governorate of Sulaimania\/Said-Sadic District. Iraqi Journal of Veterinary Sciences, 20, 19\u201323.","journal-title":"Iraqi Journal of Veterinary Sciences"},{"key":"448_CR24","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1128\/JB.06556-11","volume":"194","author":"H Tae","year":"2012","unstructured":"Tae, H., Shallom, S., Settlage, R., Hawkins, G.N., Adams, L.R., Garner, H.R., 2012. Complete genome sequence of Brucella suis VBI22, isolated from bovine milk. Journal of Bacteriology, 194, 910.","journal-title":"Journal of Bacteriology"},{"key":"448_CR25","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1017\/S0022029903006071","volume":"70","author":"GM Tantillo","year":"2003","unstructured":"Tantillo, G.M., Pinto, A.D., Buonavoglia, C., 2003. Detection of Brucella spp. in soft cheese by semi-nested polymerase chain reaction. Journal of Dairy Research, 70, 245\u2013247.","journal-title":"Journal of Dairy Research"},{"key":"448_CR26","unstructured":"The World Bank, 2008. World development indicators. Poverty data: A supplement to World development indicators. pp 1\u201326."},{"key":"448_CR27","unstructured":"World Health Organization (WHO), 2012. Neglected zoonotic diseases (NZD). In: http:\/\/www.who.int\/neglected_diseases\/zoonoses\/en\/ , Accessed 26 November, 2012."},{"key":"448_CR28","doi-asserted-by":"crossref","first-page":"306","DOI":"10.3325\/cmj.2010.51.306","volume":"51","author":"WL Yu","year":"2010","unstructured":"Yu, W.L., Nielsen, K., 2010. Review of detection of Brucella spp. by polymerase chain reaction. Croatian Medical Journal, 51, 306\u2013313.","journal-title":"Croatian Medical Journal"}],"container-title":["Tropical Animal Health and Production"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-013-0448-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s11250-013-0448-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-013-0448-6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T04:20:33Z","timestamp":1596169233000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s11250-013-0448-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,19]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["448"],"URL":"https:\/\/doi.org\/10.1007\/s11250-013-0448-6","relation":{},"ISSN":["0049-4747","1573-7438"],"issn-type":[{"value":"0049-4747","type":"print"},{"value":"1573-7438","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,19]]}}}