{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:48:38Z","timestamp":1793144918528,"version":"4.5.0"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T00:00:00Z","timestamp":1488499200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Trop Anim Health Prod"],"published-print":{"date-parts":[[2017,4]]},"DOI":"10.1007\/s11250-017-1256-1","type":"journal-article","created":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T23:04:05Z","timestamp":1488495845000},"page":"755-763","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Evaluation of PCR methods for detection of Brucella strains from culture and tissues"],"prefix":"10.1007","volume":"49","author":[{"given":"Alper","family":"\u00c7iftci","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuba","family":"\u0130\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serap","family":"Sava\u015fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bar\u0131\u015f","family":"Sareyy\u00fcpo\u011flu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet","family":"Akan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kadir Serdar","family":"Diker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,3,3]]},"reference":[{"issue":"11-12","key":"1256_CR1","first-page":"577","volume":"49","author":"S Al Dahouk","year":"2003","unstructured":"Al Dahouk, S., Tomaso, H., N\u00f6ckler, K., Neubauer, H. and Frangoulidis, D., 2003. Laboratory-based diagnosis of brucellosis\u2014a review of the literature. Part II: serological tests for brucellosis. Clinical Laboratory, 49(11-12), 577\u2013589.","journal-title":"Clinical Laboratory"},{"key":"1256_CR2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.33073\/pjm-2011-004","volume":"60","author":"HH Al-Ajlan","year":"2011","unstructured":"Al-Ajlan, H.H., Ibrahim, A.S. and Al-Salamah, A.A., 2011. Comparison of different PCR methods for detection of Brucella spp. in human blood samples. Polish Journal of Microbiology, 60, 27\u201333.","journal-title":"Polish Journal of Microbiology"},{"key":"1256_CR3","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0378-1135(01)00401-1","volume":"83","author":"AS Amin","year":"2001","unstructured":"Amin, A.S., Hamdy, M.E. and Ibrahim, A.K., 2001. Detection of Brucella melitensis in semen using the polymerase chain reaction assay. Veterinary Microbiology, 83, 37\u201344.","journal-title":"Veterinary Microbiology"},{"key":"1256_CR4","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.jiph.2012.02.001","volume":"5","author":"AM Asaad","year":"2012","unstructured":"Asaad, A.M. and Alqahtani, J.M., 2012. Serological and molecular diagnosis of human brucellosis in Najran, Southwestern Saudi Arabia. Journal of Infection and Public Health, 5, 189\u2013194.","journal-title":"Journal of Infection and Public Health"},{"issue":"1","key":"1256_CR5","first-page":"95","volume":"27","author":"JM Blasco","year":"2011","unstructured":"Blasco, J.M. and Molina-Flores B., 2011. Control and eradication of Brucella melitensis infection in sheep and goats. Veterinary Clinics of North America, 27 (1), 95\u2013104.","journal-title":"Veterinary Clinics of North America"},{"key":"1256_CR6","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.vetmic.2008.12.023","volume":"137","author":"L Bounaadja","year":"2009","unstructured":"Bounaadja, L., Albert, D., Chenais, B., Henault, S., Zygmunt, M.S., Poliak, S. and Garin-Bastuji, B., 2009. Real-time PCR for identification of Brucella spp.: a comparative study of IS711, bcsp31 and per target genes. Veterinary Microbiology, 137, 156\u2013164.","journal-title":"Veterinary Microbiology"},{"key":"1256_CR7","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1128\/JCM.32.11.2660-2666.1994","volume":"32","author":"BJ Bricker","year":"1994","unstructured":"Bricker, B.J. and Halling, S.M., 1994. Differentiation of Brucella abortus bv. 1, 2 and 4, Brucella melitensis, Brucella ovis bv. 1 by PCR. Journal of Clinical Microbiology, 32, 2660\u20132666.","journal-title":"Journal of Clinical Microbiology"},{"key":"1256_CR8","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1128\/JCM.33.6.1640-1642.1995","volume":"33","author":"BJ Bricker","year":"1995","unstructured":"Bricker, B.J. and Halling, S.M., 1995. Enhancement of Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. Journal of Clinical Microbiology, 33, 1640\u20131642.","journal-title":"Journal of Clinical Microbiology"},{"key":"1256_CR9","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/S0378-1135(02)00228-6","volume":"90","author":"BJ Bricker","year":"2002","unstructured":"Bricker, B.J., 2002. PCR as a diagnostic tool for brucellosis. Veterinary Microbiology, 90, 435\u2013436.","journal-title":"Veterinary Microbiology"},{"key":"1256_CR10","doi-asserted-by":"crossref","first-page":"55","DOI":"10.4103\/0974-2727.72149","volume":"2","author":"S Christopher","year":"2010","unstructured":"Christopher, S., Umapathy, B.L. and Ravikumar, K.L., 2010. Brucellosis: review on the recent trends in pathogenicity and laboratory diagnosis. Journal of Laboratory Physicians, 2, 55\u201360.","journal-title":"Journal of Laboratory Physicians"},{"key":"1256_CR11","first-page":"751","volume":"64","author":"A Costa","year":"2012","unstructured":"Costa, A., Sant\u2019ana, M., Carvalho, S., Moustacas, S., Silva, S., Paix\u00e3o, T.A. and Santos, R.L., 2012. Diagnosis of B. ovis infection by serology and PCR in urine samples from naturally infected rams in the State of Piau\u00ed. Brazilian Journal of Veterinary and Animal Science, 64, 751\u2013754.","journal-title":"Brazilian Journal of Veterinary and Animal Science"},{"key":"1256_CR12","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1136\/vr.144.9.239","volume":"144","author":"B \u00c7etinkaya","year":"1999","unstructured":"\u00c7etinkaya, B., \u00d6ng\u00f6r, H., Muz, A., Erta\u015f, H.B., Kalender, H. and Erdo\u011fan, H.M. 1999. Detection of Brucella species DNA in the stomach content of aborted sheep fetuses by PCR. Veterinary Record, 144, 239\u2013240.","journal-title":"Veterinary Record"},{"issue":"1","key":"1256_CR13","doi-asserted-by":"crossref","first-page":"53","DOI":"10.20506\/rst.32.1.2187","volume":"32","author":"E D\u00edaz Aparicio","year":"2013","unstructured":"D\u00edaz Aparicio, E., 2013. Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Revue Scientifique et Technique, 32 (1), 53\u201360.","journal-title":"Revue Scientifique et Technique"},{"key":"1256_CR14","first-page":"323","volume":"7","author":"MA Geresu","year":"2016","unstructured":"Geresu, M.A. and Kassa, G.M., 2016. A review on diagnostic methods of brucellosis. Journal of Veterinary Science and Technology, 7, 323.","journal-title":"Journal of Veterinary Science and Technology"},{"key":"1256_CR15","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.prevetmed.2011.04.007","volume":"102","author":"J Godfroid","year":"2011","unstructured":"Godfroid, J., Scholz, H.C., Barbier, T., Nicolas, C., Wattiau, P., Fretin, D., Whatmore, A.M., Cloeckaert, A., Blasco, J.M., Moriyon, I., Saegerman, C., Muma, J.B., Al Dahouk S., Neubauer, H. and Letesson, J., 2011. Brucellosis at the animal\/ecosystem\/human interface at the beginning of the 21st century. Preventive Veterinary Medicine, 102, 118\u2013131.","journal-title":"Preventive Veterinary Medicine"},{"key":"1256_CR16","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1128\/AEM.58.6.2099-2101.1992","volume":"58","author":"L Herman","year":"1992","unstructured":"Herman, L. and de Ridder, H., 1992. Identification of Brucella spp. by using the polymerase chain reaction. Applied and Environmental Microbiology, 58, 2099\u20132101.","journal-title":"Applied and Environmental Microbiology"},{"issue":"4","key":"1256_CR17","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1017\/S0950268807008795","volume":"136","author":"NE Lucero","year":"2008","unstructured":"Lucero, N.E., Ayala, S.M., Escobar, G.I. and Jacob, N.R., 2008. Brucella isolated in humans and animals in Latin America from 1968 to 2006. Epidemiology and Infection, 136(4), 496\u2013503.","journal-title":"Epidemiology and Infection"},{"key":"1256_CR18","doi-asserted-by":"publisher","DOI":"10.17957\/IJAB\/15.0099","author":"R Mahmood","year":"2016","unstructured":"Mahmood, R., Ali, T., Waheed, U., Asif, M. and Khan, Q.M., 2016. Application of serum based PCR and fluorescence polarization assay for diagnosis of brucellosis among people occupationally at risk to disease. International Journal of Agriculture & Biology, DOI: 10.17957\/IJAB\/15.0099.","journal-title":"International Journal of Agriculture & Biology"},{"key":"1256_CR19","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1099\/jmm.0.47160-0","volume":"56","author":"F Mukherjee","year":"2007","unstructured":"Mukherjee, F., Jain, J., Patel, V. and Nair, M., 2007. Multiple genus-specific markers in PCR assays improve the specificity and sensitivity of diagnosis of brucellosis in field animals. Journal of Medical Microbiology, 56, 1309\u20131316.","journal-title":"Journal of Medical Microbiology"},{"key":"1256_CR20","first-page":"35","volume":"20","author":"HK M\u00fc\u015ftak","year":"2009","unstructured":"M\u00fc\u015ftak, H.K., G\u00fcnayd\u0131n, E., K\u00fc\u00e7\u00fckayan, U. and Dakman, A., 2009. Diagnosis of Brucella spp. by conventional cultural method and polymerase chain reaction in stomach contents of aborted fetuses. Journal of Etlik Veterinary Microbiology, 20, 35\u201338.","journal-title":"Journal of Etlik Veterinary Microbiology"},{"key":"1256_CR21","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/j.1574-695X.2002.tb00616.x","volume":"34","author":"E Navarro","year":"2002","unstructured":"Navarro, E., Escribano, J., Fernandez, J.A. and Solera, J., 2002. Comparison of three different PCR methods for detection of Brucella spp. in human blood samples. FEMS Immunology and Medical Microbiology, 34, 147\u2013151.","journal-title":"FEMS Immunology and Medical Microbiology"},{"key":"1256_CR22","unstructured":"OIE terrestrial manual, 2009 bovine brucellosis. Retrieved February 24, 2016 from http:\/\/www.oie.int\/fileadmin\/Home\/eng\/Health_standards\/tahm\/2.04.03_BOVINE_ brucell. pdf."},{"key":"1256_CR23","doi-asserted-by":"crossref","unstructured":"Poiester, F.P., Nielsen, K., Samartino, L.E. and Yu, W.L., 2010. Diagnosis of Brucellosis. Open Veterinary Science Journal, 4, 46.","DOI":"10.2174\/1874318801004010046"},{"issue":"2","key":"1256_CR24","doi-asserted-by":"crossref","first-page":"54","DOI":"10.5897\/AJMR2015.7851","volume":"10","author":"HM Refaat","year":"2016","unstructured":"Refaat, H.M., Ahmed, O.I., Elabd, S.H. and Sanad, M.M., 2016. Polymerase chain reaction (PCR) technique compared to conventional bacteriological and serological techniques in diagnosis of human brucellosis in Egypt. African Journal of Microbiology, 10(2), 54\u201361.","journal-title":"African Journal of Microbiology"},{"key":"1256_CR25","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.mcp.2016.02.007","volume":"30","author":"H Ren","year":"2016","unstructured":"Ren, H., Yang, M., Zhang, G., Liu, S., Wang, X., Ke, Y., Du, X., Wang, Z., Huang, L., Liu, C. and Chen, Z., 2016. Development of a rapid recombinase polymerase amplification assay for detection of Brucella in blood samples. Molecular and Cellular Probes, 30, 122\u2013124.","journal-title":"Molecular and Cellular Probes"},{"key":"1256_CR26","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1128\/AEM.62.5.1683-1688.1996","volume":"62","author":"NP Rijpens","year":"1996","unstructured":"Rijpens, N.P., Jannes, G., Van Asbroeck, M., Rossau, R. and Herman, L.M., 1996. Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes. Applied and Environmental Microbiology, 62, 1683\u20131688.","journal-title":"Applied and Environmental Microbiology"},{"key":"1256_CR27","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1128\/JCM.33.3.615-617.1995","volume":"33","author":"C Romero","year":"1995","unstructured":"Romero, C., Gamazo, C., Pardo, M. and Lopez-Goni, I., 1995. Specific detection of Brucella DNA by PCR. Journal of Clinical Microbiology, 33, 615\u2013617.","journal-title":"Journal of Clinical Microbiology"},{"key":"1256_CR28","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1111\/j.1365-2672.2012.05384.x","volume":"113","author":"C Schrader","year":"2012","unstructured":"Schrader, C., Schielke, A., Ellerbroek, L. and Johne, R., 2012. PCR inhibitors\u2014occurrence, properties and removal. Journal of Applied Microbiology, 113, 1014\u20131026.","journal-title":"Journal of Applied Microbiology"},{"issue":"5","key":"1256_CR29","doi-asserted-by":"crossref","first-page":"913","DOI":"10.4314\/tjpr.v15i5.3","volume":"15","author":"A Shakerian","year":"2016","unstructured":"Shakerian, A., Deo, P., Rahimi, E., Shahjavan, A.R. and Khamesipour, F., 2016. Molecular detection of Brucella melitensis in sheep and goat milk in Iran. Tropical Journal of Pharmacological Research, 15(5), 913\u2013918.","journal-title":"Tropical Journal of Pharmacological Research"},{"key":"1256_CR30","doi-asserted-by":"crossref","first-page":"2913","DOI":"10.1099\/00221287-143-9-2913","volume":"143","author":"N Vizcaino","year":"1997","unstructured":"Vizcaino, N., Verger, J.M., Grayon, M., Zygmunt, M.S. and Cloeckaert, A., 1997. DNA polymorphism at the OMP-31 locus of Brucella spp.: evidence for a large deletion in Brucella abortus and other species-specific markers. Microbiology, 143, 2913\u20132921.","journal-title":"Microbiology"},{"issue":"1","key":"1256_CR31","first-page":"44","volume":"18","author":"M Zamanian","year":"2015","unstructured":"Zamanian, M., Hashemi Tabar, G.R., Rad, M. and Haghparast, A., 2015. Evaluation of different primers for detection of Brucella in human and animal serum samples by using PCR method. Archives of Iranian Medicine, 18(1), 44\u201350.","journal-title":"Archives of Iranian Medicine"},{"key":"1256_CR32","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1128\/JCM.39.4.1661-1664.2001","volume":"39","author":"L Zerva","year":"2001","unstructured":"Zerva, L., Bourantas, K., Mitka, S., Kansousidou, A. and Legakis, N.J., 2001. Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR. Journal of Clinical Microbiology, 39, 1661\u20131664.","journal-title":"Journal of Clinical Microbiology"}],"container-title":["Tropical Animal Health and Production"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-017-1256-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s11250-017-1256-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-017-1256-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T09:16:01Z","timestamp":1601716561000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s11250-017-1256-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,3]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["1256"],"URL":"https:\/\/doi.org\/10.1007\/s11250-017-1256-1","relation":{},"ISSN":["0049-4747","1573-7438"],"issn-type":[{"value":"0049-4747","type":"print"},{"value":"1573-7438","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,3]]}}}