{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T22:14:19Z","timestamp":1793052859395,"version":"4.5.0"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T00:00:00Z","timestamp":1599782400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T00:00:00Z","timestamp":1599782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Trop Anim Health Prod"],"published-print":{"date-parts":[[2020,11]]},"DOI":"10.1007\/s11250-020-02374-8","type":"journal-article","created":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T21:02:32Z","timestamp":1599858152000},"page":"3409-3415","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Effect of diluent sugars on capacitation status and acrosome reaction of spermatozoa in buck semen at refrigerated temperature"],"prefix":"10.1007","volume":"52","author":[{"given":"Chetna","family":"Gangwar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh Dinkar","family":"Kharche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil Kumar","family":"Mishra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Saraswat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narendra","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuj Kumar","family":"Sikarwar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,9,11]]},"reference":[{"issue":"1","key":"2374_CR1","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/0011-2240(91)90006-A","volume":"28","author":"AA Abdelhakeam","year":"1991","unstructured":"Abdelhakeam, A.A., Graham, E.F., Vazquez, I.A., Chaloner, K.M., 1991. Studies on the absence of glycerol in unfrozen and frozen ram semen: development of an extender for freezing: effects of osmotic pressure, egg yolk levels, type of sugars, and the method of dilution. Cryobiology, 28(1), 43\u201349.","journal-title":"Cryobiology"},{"key":"2374_CR2","doi-asserted-by":"publisher","first-page":"1801","DOI":"10.1016\/S0093-691X(02)00653-2","volume":"57","author":"EG Aisen","year":"2002","unstructured":"Aisen, E.G., Medina, V.H., Venturino, A., 2002. Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology, 57, 1801-1808.","journal-title":"Theriogenology"},{"issue":"3","key":"2374_CR3","first-page":"227","volume":"42","author":"S Akhter","year":"2010","unstructured":"Akhter, S., Ansari, M.S., Rakha, B.A., Andrabi, S.M.H., Anwar, M., Ullah, N., 2010. Effect of fructose addition in skim milk extender on the quality of liquid Nili-Ravi buffalo (Bubalusbubalis) semen. Pakistan Journal of Zooogyl, 42(3), 227-231.","journal-title":"Pakistan Journal of Zooogyl"},{"key":"2374_CR4","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.cryobiol.2018.02.001","volume":"81","author":"A Arangasamy","year":"2018","unstructured":"Arangasamy, A., Krishnaiah, M.V., Manohara, N., Selvaraju, S., Rania, G.P., Soren, N.M., Reddy, I.J., Ravindra, J.P., 2018. Cryoprotective role of organic Zn and Cu supplementation in goats (Capra hircus) diet. Cryobiology, 81, 117-124","journal-title":"Cryobiology"},{"key":"2374_CR5","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1111\/j.1439-0272.1993.tb02694.x","volume":"25","author":"SR Bawa","year":"1993","unstructured":"Bawa, S.R., Pabst, M.A., Werner, G., Bains, H.K., 1993. Capacitated and acrosome reacted spermatozoa of goat (Cupru indicus): a fluorescence and electron microscopic study. Andrology, 25, 123-135.","journal-title":"Andrology"},{"key":"2374_CR6","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1016\/S0015-0282(16)30125-X","volume":"1","author":"E Bloom","year":"1950","unstructured":"Bloom E. 1950.A one minute live-dead sperm stain by means of eosin-nigrosin. Fertility and Sterility, 1,176-177.","journal-title":"Fertility and Sterility"},{"key":"2374_CR7","first-page":"741","volume":"14","author":"JM Corteel","year":"1974","unstructured":"Corteel, J.M., 1974. Viabilite\u2019 des spermatozoid de bouc conserves etcongele\u2019savecou sans leur plasma seminal: effect du glucose (viability of spermatozoa deep frozen with or without seminal plasma: glucose effect). Ann BiolAnimBiochemBiophys journal, 14, 741","journal-title":"Ann BiolAnimBiochemBiophys journal"},{"key":"2374_CR8","unstructured":"Evans, G., Maxwel, W., 1987. Salamons artificial insemination of sheep and goats. Butterworths. Sydney 122-141."},{"issue":"4","key":"2374_CR9","first-page":"738","volume":"67","author":"MR Fern\u00e1ndez-Santos","year":"2007","unstructured":"Fern\u00e1ndez-Santos, M.R., Mart\u00ednez-Pastor, F., Garc\u00eda-Mac\u00edas, V., Esteso, M.C., Soler, A.J., de Paz, P., 2007.Extender osmolality and sugar supplementation exert a complex effect on the cryopreservation of Iberian red deer (Cervuselaphushispanicus) epididymal spermatozoa Theriogenology, 67(4), 738\u2013753.","journal-title":"Iberian red deer (Cervuselaphushispanicus) epididymal spermatozoa Theriogenology"},{"key":"2374_CR10","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1002\/mrd.1080400213","volume":"40","author":"LR Fraser","year":"1995","unstructured":"Fraser, L.R., Abeydeera, L.R., Niwa, K.,1995. Ca2+-regulating mechanisms that modulate bull sperm capacitation and acrosomal exocytosis as determined by chlortetracycline analysis. Molecular Reproduction Development, 40, 233-241.","journal-title":"Molecular Reproduction Development"},{"key":"2374_CR11","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/j.smallrumres.2015.06.002","volume":"129","author":"C Gangwar","year":"2015","unstructured":"Gangwar, C., Kharche, S.D., Ranjan, R., Kumar, S., Goel, A.K., Jindal, S.K., 2015. Effect of vitamin C supplementation on freezability of Barbari buck semen. Small Ruminant Research ,129, 104-107.","journal-title":"Small Ruminant Research"},{"key":"2374_CR12","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.anireprosci.2018.03.005","volume":"19","author":"C Gangwar","year":"2018","unstructured":"Gangwar, C., Saxena, A., Patel, A., Singh, S.P., Yadav, S., Kumar, R., Singh, V., 2018. Effect of reduced glutathione supplementation on cryopreservation induced sperm cryoinjuries in murrah bull semen. Animal Reproduction Science, 19, 171\u2013178.","journal-title":"Animal Reproduction Science"},{"issue":"1","key":"2374_CR13","doi-asserted-by":"publisher","first-page":"125","DOI":"10.5958\/0973-9718.2019.00060.6","volume":"26","author":"C Gangwar","year":"2020","unstructured":"Gangwar, C., Jena, D., Kharche, S.D., Kumar, N., Jindal, S.K., Ranjan, R., Goel, A.K., 2020. Effect of sugar supplementation in diluent on buck sperm characteristics at refrigeration temperature. Indian journal of small ruminant research, 26(1), 125-127.","journal-title":"Indian journal of small ruminant research"},{"key":"2374_CR14","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.smallrumres.2018.02.003","volume":"161","author":"K Hemalatha","year":"2018","unstructured":"Hemalatha, K., Arangasamy, A., Selvaraju, S.,VenkataKrishnaiah, M., Rani, G.P., Mishra, A., Soren, N.M., Reddy, I.J., Ravindra, J.P., 2018. Effect of dietary supplementation of organic zincand copper on in vitrosemen fertility in goat. Small Ruminant Research, 161, 68\u201372.","journal-title":"Small Ruminant Research"},{"key":"2374_CR15","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/S0378-4320(00)00152-4","volume":"62","author":"WV Holt","year":"2000","unstructured":"Holt, W.V., 2000. Basic aspects of frozen storage of semen. Animal Reproduction Science, 62, 3-22.","journal-title":"Animal Reproduction Science"},{"key":"2374_CR16","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1530\/jrf.0.0700219","volume":"70","author":"RS Jayendran","year":"1984","unstructured":"Jayendran, R.S., Vander-Ven, H., Perez-Pelaez, M., Crabo, B.G, Zanevld, L.J.D., 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characters. Journal of Reproduction and Fertility, 70, 219-228.","journal-title":"Journal of Reproduction and Fertility"},{"key":"2374_CR17","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1071\/BI9660655","volume":"19","author":"KR Lapwood","year":"1966","unstructured":"Lapwood, K.R., Martin, I.C.A., 1966. The use of monosaccharides, disaccharides and trisaccharides in synthetic diluents for the storage of ram spermatozoa at 37\u00b0C and 5\u00b0C. Australian Journal of Biological Science 19, 655\u2013671.","journal-title":"Australian Journal of Biological Science"},{"key":"2374_CR18","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/0378-4320(96)01544-8","volume":"42","author":"WMC Maxwell","year":"1996","unstructured":"Maxwell, W.M.C., Watson, P.F. 1996. Recent progress in the preservation of ram semen. Animal Reproduction Science, 42, 55\u201365.","journal-title":"Animal Reproduction Science"},{"key":"2374_CR19","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1530\/jrf.0.0950755","volume":"95","author":"C Mendoza","year":"1992","unstructured":"Mendoza, C., Carreras, A., Moos, J., Tesarik, J., 1992. Distinction between true acrosome reaction and degenerative acrosome loss by a one-step staining method using Pisumsativum agglutinin. Journal of Reproduction and Fertility, 95, 755-763.","journal-title":"Journal of Reproduction and Fertility"},{"key":"2374_CR20","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.anireprosci.2010.06.006","volume":"122","author":"SW Naing","year":"2010","unstructured":"Naing, S.W., Wahid, H., MohdAzam, K., Rosnina, Y., Zuki, A.B., Kazhal, S., Bukar, M.M., Thein, M., Kyaw, T., San, M.M., 2010.Effect of sugars on characteristics of Boer goat semen after cryopreservation. Animal Reproduction Science, 122, 23\u201328.","journal-title":"Animal Reproduction Science"},{"key":"2374_CR21","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1111\/and.12422","volume":"48","author":"G Nayak","year":"2016","unstructured":"Nayak, G., Vadinkar, A., Nair, S., Kalthur, S.G., D'Souza, A.S., Shetty, P.K., Mutalik, S., Shetty, M.M., Kalthur, G., Adiga, S.K., 2016.Sperm abnormalities induced by prepubertalexposure to cyclophosphamide are effectively mitigated by Moringaoleifera leaf extract. Andrology, 48, 125-136.","journal-title":"Andrology"},{"issue":"8","key":"2374_CR22","doi-asserted-by":"publisher","first-page":"1498","DOI":"10.1016\/j.theriogenology.2004.02.014","volume":"62","author":"S Ponglowhapan","year":"2004","unstructured":"Ponglowhapan, S., Ess\u00e9n-Gustavsson, B., Linde, F.C., 2004. Influence of glucose and fructose in the extender during long-term storage of chilled canine semen. Theriogenology, 62(8), 1498\u20131517.","journal-title":"Theriogenology"},{"issue":"3","key":"2374_CR23","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.smallrumres.2005.02.015","volume":"63","author":"PH Purdy","year":"2006","unstructured":"Purdy, P.H., 2006. A review on goat sperm cryopreservation. Small Ruminant Research, 63 (3): 215\u2013225.","journal-title":"Small Ruminant Research"},{"key":"2374_CR24","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1071\/BI9720605","volume":"25","author":"S Salamon","year":"1972","unstructured":"Salamon, S., Visser, D., 1972. Effect of composition of Tris-based diluent and of thawingsolution on survival of ram spermatozoa frozen by the pellet method. Australian Journal of Biological Sciences, 25, 605-618.","journal-title":"Australian Journal of Biological Sciences"},{"key":"2374_CR25","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1016\/j.theriogenology.2012.03.010","volume":"78","author":"S Selvaraju","year":"2012","unstructured":"Selvaraju, S., Sivasubramani, T., Raghavendra, B.S., Raju, P., Rao, S.B.N., Dineshkumar, D., Ravindra, J.P., 2012. Effect of dietary energy on seminal plasma insulin-like growth factor-I (IGF-I), serum IGF-I and testosterone levels, semen quality and fertility in adult rams. Theriogenology, 78, 646\u2013655","journal-title":"Theriogenology"},{"key":"2374_CR26","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1071\/R96109","volume":"9","author":"DP Windsor","year":"1997","unstructured":"Windsor, D.P., 1997. Mitochondrial function and ram sperm fertility. Reprodution Fertility and Development, 9, 279\u2013284.","journal-title":"Reprodution Fertility and Development"},{"key":"2374_CR27","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1016\/S0093-691X(00)00373-3","volume":"54","author":"Yrldtz","year":"2000","unstructured":"Yrldtz, Kaya, A., Aksoya, M., Tekeli, T., 2000. Influence of sugar supplementation of the extender on motility, viability and acrosomal integrity of dog spermatozoa during freezing. Theriogenology, 54, 579-585.","journal-title":"Theriogenology"}],"container-title":["Tropical Animal Health and Production"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-020-02374-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11250-020-02374-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-020-02374-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T23:55:32Z","timestamp":1631318132000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11250-020-02374-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,11]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["2374"],"URL":"https:\/\/doi.org\/10.1007\/s11250-020-02374-8","relation":{},"ISSN":["0049-4747","1573-7438"],"issn-type":[{"value":"0049-4747","type":"print"},{"value":"1573-7438","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,11]]},"assertion":[{"value":"12 April 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 September 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 September 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All the experiments were carried out in accordance with the ethical guidelines of the institute<b>.<\/b>","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Authors have taken consent for publishing this article from the institute.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}]}}