{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:47:31Z","timestamp":1792691251019,"version":"4.5.0"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T00:00:00Z","timestamp":1612396800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T00:00:00Z","timestamp":1612396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Trop Anim Health Prod"],"published-print":{"date-parts":[[2021,3]]},"DOI":"10.1007\/s11250-021-02589-3","type":"journal-article","created":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T03:03:05Z","timestamp":1612407785000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Behavioral and physiological responses to early weaning in ewes and their single or twin lambs"],"prefix":"10.1007","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1418-0148","authenticated-orcid":false,"given":"Aline","family":"Freitas-de-Melo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Ungerfeld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agust\u00edn","family":"Orihuela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,4]]},"reference":[{"key":"2589_CR1","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1111\/rda.12922","volume":"52","author":"S Abril-S\u00e1nchez","year":"2017","unstructured":"Abril-S\u00e1nchez, S., Freitas-de-Melo, A., Dami\u00e1n, J.P., Giriboni, J., Villagr\u00e1-Garc\u00eda, A., Ungerfeld, R., 2017. Ejaculation does not contribute to the stress response to electroejaculation in sheep. Reproduction in Domestic Animals; 52: 403\u2013408. https:\/\/doi.org\/10.1111\/rda.12922.","journal-title":"Reproduction in Domestic Animals"},{"key":"2589_CR2","doi-asserted-by":"publisher","first-page":"71","DOI":"10.2527\/1993.71171x","volume":"71","author":"JK Apple","year":"1993","unstructured":"Apple, J. K., Minton, J. E., Parsons, K. M., & Unruh, J. A., 1993. Influence of repeated restraint and isolation stress and electrolyte administration on pituitary-adrenal secretions, electrolytes, and other blood constituents of sheep. Journal of animal science; 71: 71-77. https:\/\/doi.org\/10.2527\/1993.71171x","journal-title":"Journal of animal science"},{"key":"2589_CR3","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/0168-1591(93)90061-S","volume":"38","author":"MS Cockram","year":"1993","unstructured":"Cockram, M.S., Imlah, P., Goddard, P.J., Harkiss, G.D., Waran, N.K., 1993. The behavioural, endocrine and leucocyte response of ewes to repeated removal of lambs before the age of natural weaning. Applied Animal Behaviour Science; 38: 127\u2013142. https:\/\/doi.org\/10.1016\/0168-1591(93)90061-S","journal-title":"Applied Animal Behaviour Science"},{"key":"2589_CR4","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1016\/j.rvsc.2013.08.001","volume":"95","author":"JP Dami\u00e1n","year":"2013","unstructured":"Dami\u00e1n, J.P., H\u00f6tzel, M.J., Banchero, G., Ungerfeld, R., 2013. Behavioural response of grazing lambs to changes associated with feeding and separation from their mothers at weaning. Research in Veterinary Science; 95: 913\u2013918. https:\/\/doi.org\/10.1016\/j.rvsc.2013.08.001","journal-title":"Research in Veterinary Science"},{"key":"2589_CR5","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/B978-0-08-100718-1.00007-8","volume-title":"Advances in Sheep Welfare","author":"C Dwyer","year":"2017","unstructured":"Dwyer, C., 2017. Reproductive management (including impacts of prenatal stress on offspring development). In: (Ed. M.D. Ferguson, C. Lee, A. Fisher), Advances in Sheep Welfare, Elsevier, United Kingdom, pp. 131\u2013149."},{"issue":"3-4","key":"2589_CR6","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1016\/S0168-1591(97)00148-2","volume":"58","author":"CM Dwyer","year":"1998","unstructured":"Dwyer, C.M., Lawrence, A.B., 1998. Variability in the expression of maternal behaviour in primiparous sheep: effects of genotype and litter size. Applied Animal Behaviour Science; 58:3-4 311\u2013330. https:\/\/doi.org\/10.1016\/S0168-1591(97)00148-2","journal-title":"Applied Animal Behaviour Science"},{"key":"2589_CR7","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1016\/j.rvsc.2010.09.020","volume":"91","author":"B Earley","year":"2011","unstructured":"Earley, B., McDonnell, B., Murray, M., Prendiville, D. J., & Crowe, M. A. 2011. The effect of sea transport from Ireland to the Lebanon on inflammatory, adrenocortical, metabolic and behavioural responses of bulls. Research in veterinary science; 91: 454-464. https:\/\/doi.org\/10.1016\/j.rvsc.2010.09.020","journal-title":"Research in veterinary science"},{"key":"2589_CR8","doi-asserted-by":"publisher","first-page":"185","DOI":"10.22319\/rmcp.v7i2.4173","volume":"7","author":"A Freitas-de-Melo","year":"2016","unstructured":"Freitas-de-Melo, A., Ungerfeld, R., 2016a. Progesterona y respuesta de estr\u00e9s: mecanismos de acci\u00f3n y sus repercusiones en rumiantes dom\u00e9sticos. Revisi\u00f3n (Progesterone and stress response: mechanisms of action and its impact in domestic ruminants. Review). Revista mexicana de ciencias pecuarias; 7: 185-199. https:\/\/doi.org\/10.22319\/rmcp.v7i2.4173","journal-title":"Revista mexicana de ciencias pecuarias"},{"key":"2589_CR9","doi-asserted-by":"publisher","first-page":"361","DOI":"10.22319\/rmcp.v7i3.4215","volume":"7","author":"A Freitas-de-Melo","year":"2016","unstructured":"Freitas-de-Melo, A., Ungerfeld R., 2016b. Destete artificial en ovinos: respuesta de estr\u00e9s y bienestar animal (Artificial weaning in sheep: stress response and animal welfare). Revista mexicana de ciencias pecuarias; 7: 361\u2013375. https:\/\/doi.org\/10.22319\/rmcp.v7i3.4215","journal-title":"Revista mexicana de ciencias pecuarias"},{"key":"2589_CR10","doi-asserted-by":"publisher","unstructured":"Freitas-de-Melo, A. Ungerfeld, R., 2020. The sex of the offspring affects the lamb and ewe responses to abrupt weaning. Applied Animal Behaviour Science; 229: 105008. https:\/\/doi.org\/10.1016\/j.applanim.2020.105008.","DOI":"10.1016\/j.applanim.2020.105008"},{"key":"2589_CR11","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1017\/S1751731113000621","volume":"8","author":"A Freitas-de-Melo","year":"2013","unstructured":"Freitas-de-Melo, A., Banchero, G., H\u00f6tzel, M.J., Dami\u00e1n, J.P., Ungerfeld, R., 2013. Progesterone administration reduces the behavioural and physiological responses of ewes to abrupt weaning of lambs. Animal; 8: 1367\u20131373. https:\/\/doi.org\/10.1017\/S1751731113000621.","journal-title":"Animal"},{"key":"2589_CR12","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1017\/S1751731116001427","volume":"11","author":"A Freitas-de-Melo","year":"2017","unstructured":"Freitas-de-Melo, A., Ungerfeld, R., H\u00f6tzel, M.J., Orihuela, A., P\u00e9rez-Clariget, R., 2017. Low pasture allowance until late gestation in ewes: behavioural and physiological changes in ewes and lambs from lambing to weaning. Animal; 11: 285\u2013294. https:\/\/doi.org\/10.1017\/S1751731116001427.","journal-title":"Animal"},{"key":"2589_CR13","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.jveb.2018.10.007","volume":"30","author":"A Freitas-de-Melo","year":"2019","unstructured":"Freitas-de-Melo, A., Ungerfeld, R., P\u00e9rez-Clariget, R., 2019. Behavioral pattern in Texel x Corriedale terminal crossbreeding: Maternal behavior score at birth, lambs\u2019 feeding behaviors, and behavioral responses of lambs to abrupt weaning, Journal of Veterinary Behavavior; 30: 9\u201315. https:\/\/doi.org\/10.1016\/j.jveb.2018.10.007.","journal-title":"Journal of Veterinary Behavavior"},{"key":"2589_CR14","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/0168-1591(89)90078-6","volume":"22","author":"GN Hinch","year":"1989","unstructured":"Hinch, G.N., 1989. The sucking behaviour of triplet, twin and single lambs at pasture. Applied Animal Behaviour Science; 22: 39\u201348. https:\/\/doi.org\/10.1016\/0168-1591(89)90078-6","journal-title":"Applied Animal Behaviour Science"},{"key":"2589_CR15","first-page":"211","volume":"41","author":"PG Keefe","year":"2000","unstructured":"Keefe P.G., Wichtel, J.J., 2000. Evaluation of melengestrol acetate and equine chorionic gonadotropin for out-of-season breeding in sheep on Prince Edward Island. The Canadian Veterinary Journal; 41: 211\u2013214.","journal-title":"The Canadian Veterinary Journal"},{"key":"2589_CR16","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.anireprosci.2012.08.021","volume":"134","author":"L Lacuesta","year":"2012","unstructured":"Lacuesta, L., Ungerfeld, R., 2012. Sexual performance and stress response of previously unknown rams after grouping them in dyads. Animal reproduction science; 134: 158\u2013163. https:\/\/doi.org\/10.1016\/j.anireprosci.2012.08.021","journal-title":"Animal reproduction science"},{"key":"2589_CR17","volume-title":"Handbook of ethological methods","author":"PN Lehner","year":"1996","unstructured":"Lehner, P.N., 1996. Handbook of ethological methods, 2nd edn, Cambridge University Press, Cambridge, UK.","edition":"2"},{"key":"2589_CR18","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1016\/j.jveb.2014.11.008","volume":"10","author":"A Maldonado","year":"2015","unstructured":"Maldonado, A., Orihuela, A., Aguirre, V., V\u00e1zquez, R., Flores-P\u00e9rez, I., 2015. Changes in mother-offspring relationships with the increasing age of the lamb in hair sheep (Ovis aries). Journal of Veterinary Behavior; 10: 166\u2013170. https:\/\/doi.org\/10.1016\/j.jveb.2014.11.008","journal-title":"Journal of Veterinary Behavior"},{"key":"2589_CR34","doi-asserted-by":"publisher","unstructured":"Napolitano, F., De Rosa, G., Sevi, A., 2008. Welfare implications of artificial rearing and early weaning in sheep. Applied Animal Behaviour Science; 110: 58\u201372. https:\/\/doi.org\/10.1016\/j.applanim.2007.03.020","DOI":"10.1016\/j.applanim.2007.03.020"},{"key":"2589_CR20","volume-title":"Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids and New World Camelids","author":"National Research Council (NRC)","year":"2007","unstructured":"National Research Council (NRC), 2007. Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids and New World Camelids. National Academy Press, Washington, D.C., USA."},{"key":"2589_CR21","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1002\/dev.420230307","volume":"23","author":"R Nowak","year":"1990","unstructured":"Nowak, R., 1990. Mother and sibling discrimination at a distance by three-to seven-day-old lambs. Developmental Psychobiology: The Journal of the International Society for Developmental Psychobiology; 23: 285\u2013295.","journal-title":"Developmental Psychobiology: The Journal of the International Society for Developmental Psychobiology"},{"key":"2589_CR22","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1002\/dev.420230307","volume":"8","author":"R Nowak","year":"1989","unstructured":"Nowak, R., Poindron, P., Putu, I.G., 1989. Development of mother discrimination by single and multiple newborn lambs. Developmental Psychobiology: The Journal of the International Society for Developmental Psychobiology; 8: 833\u2013845. https:\/\/doi.org\/10.1002\/dev.420230307","journal-title":"Developmental Psychobiology: The Journal of the International Society for Developmental Psychobiology"},{"key":"2589_CR23","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1016\/S0168-1591(05)80071-1","volume":"33","author":"CE O\u2019Connor","year":"1992","unstructured":"O\u2019Connor, C. E., Lawrence, A. B., Wood-Gush, D.G.M., 1992. Influence of litter size and parity on maternal behaviour at parturition in Scottish Blackface sheep. Applied Animal Behaviour Science; 33; 345\u2013355. https:\/\/doi.org\/10.1016\/S0168-1591(05)80071-1","journal-title":"Applied Animal Behaviour Science"},{"key":"2589_CR24","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1051\/rnd:19990208","volume":"39","author":"P Orgeur","year":"1999","unstructured":"Orgeur, P., Bernard, S., Naciri, M., Nowak, R., Schaal, B. Levy, F., 1999. Psycological consequences of two different weaning methods in sheep. Reproduction Nutrition Development; 39: 231\u2013244. https:\/\/doi.org\/10.1051\/rnd:19990208","journal-title":"Reproduction Nutrition Development"},{"key":"2589_CR25","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/0921-4488(92)90162-W","volume":"7","author":"R Peeters","year":"1992","unstructured":"Peeters, R., Buys, N., Robijns, L., Vanmontfort, D., Van Isterdael, J., 1992. Milk yield and milk composition of Flemish Milksheep, Suffolk and Texel ewes and their crossbreds. Small Ruminant Research; 7; 279\u2013288. https:\/\/doi.org\/10.1016\/0921-4488(92)90162-W","journal-title":"Small Ruminant Research"},{"key":"2589_CR26","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1016\/0304-4165(79)90275-7","volume":"584","author":"BO Phillippy","year":"1979","unstructured":"Phillippy, B.O., McCarthy, R.D., 1979. Multi-origins of milk serum albumin in the lactating goat. Biochimica et Biophysica Acta (BBA)-General Subjects; 584: 298\u2013303. https:\/\/doi.org\/10.1016\/0304-4165(79)90275-7","journal-title":"Biochimica et Biophysica Acta (BBA)-General Subjects"},{"key":"2589_CR27","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1017\/S000335610002211X","volume":"6","author":"AF Purser","year":"1964","unstructured":"Purser, A. F., Young, G.B., 1964. Mortality among twin and single lambs. Animal Science; 6: 321\u2013329. https:\/\/doi.org\/10.1017\/S000335610002211X","journal-title":"Animal Science"},{"key":"2589_CR28","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.applanim.2011.10.002","volume":"136","author":"JL Rault","year":"2012","unstructured":"Rault, J.L., 2012. Friends with benefits: social support and its relevance for farm animal welfare. Applied Animal Behaviour Science; 136: 1\u201314. https:\/\/doi.org\/10.1016\/j.applanim.2011.10.002","journal-title":"Applied Animal Behaviour Science"},{"key":"2589_CR29","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.applanim.2010.04.014","volume":"125","author":"C Schichowski","year":"2010","unstructured":"Schichowski, C., Moors, E., Gauly, M., 2010. Influence of weaning age and an experimental Haemonchus contortus infection on behaviour and growth rates of lambs. Applied Animal Behaviour Science; 125: 103\u2013108. https:\/\/doi.org\/10.1016\/j.applanim.2010.04.014","journal-title":"Applied Animal Behaviour Science"},{"key":"2589_CR30","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1016\/S0034-5288(18)32792-9","volume":"27","author":"AH Shubber","year":"1979","unstructured":"Shubber, A. H., Doxey, D. L., Black, W. J. M., FitzSimons, J., 1979. Colostrum production by ewes and the amounts ingested by lambs. Research in Veterinary Science; 27: 280\u2013282. https:\/\/doi.org\/10.1016\/S0034-5288(18)32792-9","journal-title":"Research in Veterinary Science"},{"key":"2589_CR31","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1071\/AN09037","volume":"49","author":"R Ungerfeld","year":"2009","unstructured":"Ungerfeld, R., Quintans, G., Enr\u00edquez, D. H., & H\u00f6tzel, M. J., 2009. Behavioural changes at weaning in 6-month-old beef calves reared by cows of high or low milk yield. Animal Production Science; 49: 637\u2013642. https:\/\/doi.org\/10.1071\/AN09037","journal-title":"Animal Production Science"},{"key":"2589_CR32","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1017\/S0003356100020110","volume":"53","author":"SA Vulich","year":"1991","unstructured":"Vulich, S.A., O\u2019Riordan, E.G., Hanrahan, J.P., 1991. Effect of litter size on herbage intake at pasture by ewes and their progeny. Animal Science; 53: 191\u2013197. https:\/\/doi.org\/10.1017\/S0003356100020110","journal-title":"Animal Science"},{"key":"2589_CR33","doi-asserted-by":"publisher","unstructured":"Walser, E.S., Hague, P., Yeomans, M., 1985. Preferences for sibling or mother in Dalesbred and Jacob twin lambs. Reproductive and Developmental Behaviour in Sheep; 393-401. https:\/\/doi.org\/10.1016\/B978-0-444-42444-0.50041-3","DOI":"10.1016\/B978-0-444-42444-0.50041-3"}],"container-title":["Tropical Animal Health and Production"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-021-02589-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11250-021-02589-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-021-02589-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,4]],"date-time":"2021-06-04T10:16:36Z","timestamp":1622801796000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11250-021-02589-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,4]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["2589"],"URL":"https:\/\/doi.org\/10.1007\/s11250-021-02589-3","relation":{},"ISSN":["0049-4747","1573-7438"],"issn-type":[{"value":"0049-4747","type":"print"},{"value":"1573-7438","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,4]]},"assertion":[{"value":"14 May 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 January 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 February 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All procedures were approved by the Ethics Committee for Animal Experimentation of the Universidad Aut\u00f3noma del Estado de Morelos, Mexico. All animals were handled according to the principles stated in the 86\/609\/EEC EC Directive regarding the protection of animals used for experimental and other scientific purposes, and in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments. The manuscript does not contain clinical studies or patient data.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"150"}}