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Egyptian camels are susceptible to <jats:italic>Babesia<\/jats:italic> infection; however, just a few cases have been documented. This study aimed to identify <jats:italic>Babesia<\/jats:italic> species, specifically <jats:italic>Babesia microti<\/jats:italic>, and their genetic diversity in dromedary camels in Egypt and associated hard ticks. Blood and hard tick samples were taken from 133 infested dromedary camels slaughtered in Cairo and Giza abattoirs. The study was conducted from February to November 2021. The <jats:italic>18S<\/jats:italic> rRNA gene was amplified by polymerase chain reaction (PCR) to identify <jats:italic>Babesia<\/jats:italic> species. Nested PCR targeting the \u03b2-tubulin gene was used to identify <jats:italic>B. microti<\/jats:italic>. The PCR results were confirmed by DNA sequencing. Phylogenetic analysis based on the \u00df-tubulin gene was used to detect and genotype <jats:italic>B. microti<\/jats:italic>. Three tick genera were identified in infested camels (<jats:italic>Hyalomma<\/jats:italic>, <jats:italic>Rhipicephalus<\/jats:italic>, and <jats:italic>Amblyomma<\/jats:italic>). <jats:italic>Babesia<\/jats:italic> species were detected in 3 out of 133 blood samples (2.3%), while <jats:italic>Babesia<\/jats:italic> spp. were not detected in hard ticks by using the <jats:italic>18S<\/jats:italic> rRNA gene. <jats:italic>B. microti<\/jats:italic> was identified in 9 out of 133 blood samples (6.8%) and isolated from <jats:italic>Rhipicephalus annulatus<\/jats:italic> and <jats:italic>Amblyomma cohaerens<\/jats:italic> by the \u03b2-tubulin gene. The phylogenetic analysis of the \u03b2-tubulin gene revealed that USA-type <jats:italic>B. microti<\/jats:italic> was prevalent in Egyptian camels<jats:italic>.<\/jats:italic> The results of this study suggested that the Egyptian camels may be infected with <jats:italic>Babesia<\/jats:italic> spp. and the zoonotic <jats:italic>B. microti<\/jats:italic> strains, which pose a potential risk to public health.<\/jats:p>","DOI":"10.1007\/s11250-023-03507-5","type":"journal-article","created":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T16:41:57Z","timestamp":1677170517000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Molecular detection of Babesia microti in dromedary camels in Egypt"],"prefix":"10.1007","volume":"55","author":[{"given":"Radwa","family":"Ashour","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7579-5432","authenticated-orcid":false,"given":"Dalia","family":"Hamza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"Kadry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha A.","family":"Sabry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,2,20]]},"reference":[{"key":"3507_CR1","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.14202\/vetworld.2016.1087-1101","volume":"9","author":"HH Abdullah","year":"2016","unstructured":"Abdullah, H.H. et al., 2016. 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IACUC (Vet CU28\/04\/2021\/303).All experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of the Faculty of Veterinary Medicine, Cairo University, Egypt.The study was carried out in compliance with the ARRIVE guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"91"}}