{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:31:58Z","timestamp":1791829918313,"version":"4.3.4"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T00:00:00Z","timestamp":1683158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T00:00:00Z","timestamp":1683158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100022282","name":"University of Applied and Environmental Sciences","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100022282","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Trop Anim Health Prod"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The objective of the present study was to evaluate the effects of increasing doses of protease on broilers from 1 to 42\u00a0days of age. A total of 1290 Ross AP broilers were used, distributed among five treatments: positive control diet, negative control diet (NC), NC\u2009+\u200950\u00a0ppm of protease, NC\u2009+\u2009100\u00a0ppm of protease, and NC\u2009+\u2009200\u00a0ppm of protease. Each treatment contained six replicates of 43 animals each. The inclusion of proteases in the diet had effects (\n                    <jats:italic>P\u2009<\/jats:italic>\n                    &lt;\u20090.05) on body weight, feed intake, weight gain, and feed conversion in the 12 to 21\u00a0day period; body weight, weight gain, and feed intake in the 29 to 42\u00a0day period; nutrient digestibility (energy metabolizability coefficient and crude protein at 28\u00a0days); and intestinal parameters (crypt and muscle width of jejunum and ileum at 28\u00a0days and villus length, crypt length, and jejunum thickness muscle layer at 42\u00a0days). These results indicate that the inclusion of protease in broiler feed can improve production parameters when the amount of crude protein in the diet is reduced.\n                  <\/jats:p>","DOI":"10.1007\/s11250-023-03562-y","type":"journal-article","created":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T11:01:41Z","timestamp":1683198101000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effect of exogenus protease on performance, nutrient digestibility, intestinal histomorphometric, meat quality characteristics, carcass yield in broilers fed low protein diets"],"prefix":"10.1007","volume":"55","author":[{"given":"C. F.","family":"Duque-Ram\u00edrez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A.","family":"Javierre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. M.","family":"Pe\u00f1uela-Sierra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9442-8395","authenticated-orcid":false,"given":"M.","family":"Diaz-Vargas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,5,4]]},"reference":[{"key":"3562_CR1","doi-asserted-by":"publisher","first-page":"2281","DOI":"10.3382\/ps.2011-01482","volume":"90","author":"CR Angel","year":"2011","unstructured":"Angel, C.R,. Saylor, W., Vieira, S.L., Ward, N., 2011. Effects of a monocomponent protease on performance and protein utilization in 7- to 22-day-old broiler chickens. Poultry Science, 90, 2281\u20132286.","journal-title":"Poultry Science"},{"key":"3562_CR2","unstructured":"AOAC., 2007. Official Methods of Analysis. 18th edition. Arlington Association of Official Chemists. Gaithersburg, MD."},{"key":"3562_CR3","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1079\/WPS20000024","volume":"56","author":"MR Bedford","year":"2000","unstructured":"Bedford, M.R. (2000). Removal of antibiotic growth promoters from poultry diets: Implications and strategies to minimize subsequent problems. Worlds Poultry Science Journal, 56: 347-365","journal-title":"Worlds Poultry Science Journal"},{"key":"3562_CR4","doi-asserted-by":"publisher","first-page":"2906","DOI":"10.3382\/ps\/pez038","volume":"98","author":"D Borda-Molina","year":"2019","unstructured":"Borda-Molina, D., Zuber, T., Siegert, W., Camarinha-Silva, A.D., Feuerstein, D., Rodehutscord M., 2019. Effects of protease and phytase supplements on small intestinal microbiota and amino acid digestibility in broiler chickens. Poultry Science, 98, 2906\u20132918","journal-title":"Poultry Science"},{"key":"3562_CR5","doi-asserted-by":"publisher","first-page":"1693","DOI":"10.3382\/ps.0741693","volume":"74","author":"M Boulianne","year":"1995","unstructured":"Boulianne, M., King, A.J., 1995. Biochemical and color characteristics of skinless bone less papel chicken breast meat. Poultry Science, 74, 1693-1698.","journal-title":"Poultry Science"},{"key":"3562_CR6","doi-asserted-by":"publisher","first-page":"291","DOI":"10.4314\/sajas.v50i2.12","volume":"50","author":"DP Carvalho","year":"2020","unstructured":"Carvalho, D.P., Leandro, N.S.M., Andrade, M.A., de Oliveira, H.F., Pires, M.F., Teixeira, K.A., Assun\u00e7\u00e3o, P.S., Stringhini, J.H., 2020. Protease inclusion in plant-and animal-based broiler diets: Performance, digestibility and biometry of digestive organs. South African Journal of Animal Science, 50, 291-301","journal-title":"South African Journal of Animal Science"},{"key":"3562_CR7","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1177\/011542650802300116","volume":"23","author":"MC Cave","year":"2008","unstructured":"Cave MC, Hurt RT, Frazier TH, Matheson PJ, Garrison RN, McClain CJ et al. Obesity, inflammation, and the potential application of pharmaconutrition. Nutricion Clinica Practice 2008;23:16-34.","journal-title":"Nutricion Clinica Practice"},{"key":"3562_CR8","doi-asserted-by":"publisher","first-page":"840","DOI":"10.5187\/jast.2020.62.6.840","volume":"62","author":"HM Cho","year":"2020","unstructured":"Cho, H.M., Hong, J. S. Kim, Y.N., Nawarathne, S.R., Seoung, E.I., Choi, I., Yi, J., Wu, D., Lee, H., Han, S.E., Nam, K.T., Heo, J.M., 2020. Responses in growth performance and nutrient digestibility to a multi-protease supplementation in amino acid-deficient broiler diets. Journal Animal Science Technology, 62, 840-853.","journal-title":"Journal Animal Science Technology"},{"key":"3562_CR9","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1080\/00071660410001715894","volume":"45","author":"I Ciftci","year":"2004","unstructured":"Ciftci, I., Ceylan, N., 2004. Effects of dietary threonine and crude protein on growth performance, carcase and meat composition of broilerchickens. British Poultry Science, 45, 280\u2013289.","journal-title":"British Poultry Science"},{"key":"3562_CR10","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1016\/j.anifeedsci.2004.11.007","volume":"118","author":"A Corzo","year":"2005","unstructured":"Corzo, A., Fritts, C., Kidd, M., Kerr, B., 2005. Response of broiler chicks to essential and non-essential amino acid supplementation of low crude protein diets. Animal Feed Science and Technology. 118, 319\u2013327.","journal-title":"Animal Feed Science and Technology."},{"key":"3562_CR11","unstructured":"Cowieson y Kluenter, (2018). Contribuci\u00f3n de enzimas ex\u00f3genas para potenciar la eliminaci\u00f3n de promotores de crecimiento de antibi\u00f3ticos en la producci\u00f3n av\u00edcola. Animal Feed Science Technology, 250, pp. 91 \u2013 92"},{"key":"3562_CR12","doi-asserted-by":"crossref","unstructured":"Dessimoni, G.V., Dal\u00f3lio, F.S., Moreira, J., Teixeira, L.V., Bertechini, A.G., Hermes, R.G., 2019. Protease Supplementation under Amino Acid Reduction in Diets Formulated with Different Nutritional Requirements for Broilers. ISSN 1516\u2013635X, 21, 001\u2013008.","DOI":"10.1590\/1806-9061-2017-0707"},{"key":"3562_CR13","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.livsci.2016.09.002","volume":"193","author":"XM Ding","year":"2016","unstructured":"Ding, X.M., Li, D.D., Li, Z.R., Wang, J.P., Zeng, Q.F., Bai, S.P., Su, Z.W., Zhang, K.Y., 2016. Effects of dietary crude protein levels and exogenous protease on performance, nutrient digestibility, trypsin activity and intestinal morphology in broilers, Livestock Science. 193, 26-31.","journal-title":"Livestock Science."},{"key":"3562_CR14","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1089\/ind.2012.1531","volume":"8","author":"V Glitso","year":"2012","unstructured":"Glitso, V., Pontopiddan, K., Knap, I., Ward, N., 2012. Catalyzing Innovation: Development of a Feed Protease. Industrial Biotechnology, 8, 172-175.","journal-title":"Industrial Biotechnology"},{"key":"3562_CR15","unstructured":"Grimes, J.L., 2011. The use of proteases in poultry nutrition. I Congresso sobre aditivos na alimenta\u00e7\u00e3o animal - Enzimas - IAC - Campinas, SP. Anais. Campinas: CBNA."},{"key":"3562_CR16","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1007\/s11250-020-02466-5","volume":"53","author":"A Jabbar","year":"2021","unstructured":"Jabbar, A., Tahir, M., Khan, R., Ahmad, N., 2021. Interactive effect of exogenous protease enzyme and dietary crude protein levels on growth and digestibility indices in broiler chickens during the starter phase. Tropical Animal Health and Production, 53, 23","journal-title":"Tropical Animal Health and Production"},{"key":"3562_CR17","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.aaspro.2015.08.062","volume":"6","author":"NF Kamel","year":"2015","unstructured":"Kamel, N.F., Ragaa, N.M., El-Banna, R.A., Mohamed, F.F., 2015. Effects of a monocomponent protease on performance parameters and protein digestibility in broiler chickens. Agriculture and Agricultural Science Procedia, 6, 216-225.","journal-title":"Agriculture and Agricultural Science Procedia"},{"key":"3562_CR18","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.anifeedsci.2012.04.001","volume":"175","author":"N Khodambashi-Emami","year":"2012","unstructured":"Khodambashi-Emami, N., Samie, A., Rahmani, H.R., Ruiz-Feriab, C.A., 2012. The effect of peppermint essential oil and frutooligosaccharides, as alternatives to virginiamycin, on growth performance, digestibility, gut morphology and immune response of male broilers. Animal Feed Science Technology, 175, 57-64","journal-title":"Animal Feed Science Technology"},{"key":"3562_CR19","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1111\/j.1745-4514.2001.tb00740.x","volume":"25","author":"R Olivo","year":"2001","unstructured":"Olivo, R., Soares, A.L., Ida, E.I., Shimokomaki, M., 2001. Dietary vitamin e inhibits poultry pse and improves meat functional properties. Journal Food Biochemistry, 25, 271-283.","journal-title":"Journal Food Biochemistry"},{"key":"3562_CR20","unstructured":"Peluzio, M.C.G., Batista, E.S., 2008. Prote\u00ednas. In: Costa, N.M.B.; Peluzio, M.C.G. Nutri\u00e7\u00e3o B\u00e1sica e Metabolismo. Vi\u00e7osa: Editora UFV, 120\u2013154"},{"key":"3562_CR21","doi-asserted-by":"crossref","unstructured":"Santos, T., Barbieri, A., Cardoso, R., Polycarpo, G., Pinheiro, D.F., Cruz-Polycarpo, V., 2018. Performance, gut morphometry and enzymatic activity of broilers fed neonatal supplementation in housing. Acta Scientiarum. Animal Sciences, 40, e38423.","DOI":"10.4025\/actascianimsci.v40i1.38423"},{"key":"3562_CR22","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.3382\/ps.0721146","volume":"72","author":"MO Smith","year":"1993","unstructured":"Smith, M.O., 1993. Parts yield of broilers reared under cycling high temperatures. Poultry Science, 72, 1146-1150.","journal-title":"Poultry Science"},{"key":"3562_CR23","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1093\/ps\/77.1.75","volume":"77","author":"Z Uni","year":"1998","unstructured":"Uni, Z., Ganot, S., Sklan, D., 1998. Posthatch development of mucosal function in the broiler small intestine. Poultry Science,\u00a077, 75-82.","journal-title":"Poultry Science"},{"key":"3562_CR24","doi-asserted-by":"crossref","unstructured":"Van Laack, R.L., Liu, C. H. Smith, M.O. y Loveday, H.D., 2000. Characteristics of pale, soft, exudative broiler breast meat. Poultry Science, 79, 1057-1061.","DOI":"10.1093\/ps\/79.7.1057"},{"key":"3562_CR25","doi-asserted-by":"publisher","first-page":"3598","DOI":"10.2527\/jas.2017.1648","volume":"95","author":"J Weir","year":"2017","unstructured":"Weir, J., Li, H., Warren, L.K., Macon, E., Wickens, C., 2017. Characterizing ammonia emissions from horses fed different crude protein concentrations. Journal of Animal Science, 95, 3598\u20133608.","journal-title":"Journal of Animal Science"},{"key":"3562_CR26","doi-asserted-by":"publisher","first-page":"e0173941","DOI":"10.1371\/journal.pone.0173941","volume":"12","author":"L Yuan","year":"2017","unstructured":"Yuan, L., Wang, M., Zhang, X., Wang, Z., 2017. Effects of protease and non-starch polysaccharide enzyme on performance, digestive function, activity and gene expression of endogenous enzymes of broilers. Plos One.12, e0173941.","journal-title":"Plos One."},{"key":"3562_CR27","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1093\/ps\/78.4.561","volume":"78","author":"I Zanella","year":"1999","unstructured":"Zanella, I., Sakomura, N.K., Silverside, F.G., Fiqueirdo, A., Pack, M., 1999. Effect of enzyme supplementation of broiler Diets based on corn and soybeans. Poultry science. 78, 561\u2013575.","journal-title":"Poultry Science."},{"key":"3562_CR28","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1016\/j.aninu.2015.10.003","volume":"1","author":"J Zuo","year":"2015","unstructured":"Zuo, J., Feng, D., Ling, B., Long, L., Li, T., Lahaye, L., Yang, C,. 2015. Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets. Animal Nutrition. 1, 276-282.","journal-title":"Animal Nutrition."}],"container-title":["Tropical Animal Health and Production"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-023-03562-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11250-023-03562-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-023-03562-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T02:30:23Z","timestamp":1686277823000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11250-023-03562-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,4]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["3562"],"URL":"https:\/\/doi.org\/10.1007\/s11250-023-03562-y","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-2188120\/v1","asserted-by":"object"}]},"ISSN":["0049-4747","1573-7438"],"issn-type":[{"value":"0049-4747","type":"print"},{"value":"1573-7438","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,4]]},"assertion":[{"value":"20 October 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 March 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 May 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"190"}}