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Treatment diets were supplemented 60 days before semen collection to afford proper spermatogenesis, adaptation to the feed formulated and fed throughout the study. Semen was collected with the use of artificial vagina following dietary supplementation with five treatment diets (neg. cont. \u2013 negative control, pos. cont. \u2013 positive control, FLO \u2013 5% Flaxseed oil, ASA \u2013 4% Ascorbic acid, and FLO\u2009+\u2009ASA). Semen was then extended using tris-based extender and cryopreserved using the programmable freezer (CBS Freezer 2100 series, Laboratory consumables &amp; chemical suppliers, America). Ovaries were collected from a neighbouring slaughter house and conveyed to the lab in 0.9% saline at 37\u00a0\u00b0C. Data (sperm parameters and in vitro fertility) was then exposed to the GLM (General Linear Model) in Minitab 17. Pearson\u2019s correlation coefficient was utilized to investigate the relationship between cryopreserved sperm quality and in vitro fertility. The student Least Significant Difference Test was used to separate the treatment means, and differences were accepted when the <jats:italic>p<\/jats:italic>-value was less than 0.05. The FLO\u2009+\u2009ASA group had higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) progressive (36.33\u2009\u00b1\u20091.87), total (88.24\u2009\u00b1\u20092.24), rapid motility (27.52\u2009\u00b1\u20091.74), intact plasma membrane (75.67\u2009\u00b1\u20092.08), total fertilization (65.98\u2009\u00b1\u20097.39), and total cleavage (66.19\u2009\u00b1\u20096.50)\u00a0when compared to other treatment groups. Total fertilization rate had a medium significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) medium correlation with the progressive motility (r2\u2009=\u20090.435), total motility (r<jats:sup>2<\/jats:sup>\u2009=\u20090.447) and rapid motility (r<jats:sup>2<\/jats:sup>\u2009=\u20090.409). In conclusion, dietary flaxseed and ascorbic acid (FLO\u2009+\u2009ASA) improves cryopreserved semen quality, in vitro fertilization rate, and the total cleavage rate. Noteworthy, the progressive, total and rapid motility play a crucial in the in vitro fertilization rate.<\/jats:p>","DOI":"10.1007\/s11250-024-04057-0","type":"journal-article","created":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T05:01:36Z","timestamp":1720587696000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Dietary supplementing South African indigenous rams with flaxseed oil and ascorbic acid improves cryopreserved semen quality and in vitro fertility"],"prefix":"10.1007","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7096-3660","authenticated-orcid":false,"given":"Jabulani Nkululeko","family":"Ngcobo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tshimangadzo Lucky","family":"Nedambale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khathutshelo Agree","family":"Nephawe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sindisiwe Mbali","family":"Sithole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tlou Caswell","family":"Chokoe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fhulufhelo Vincent","family":"Ramukhithi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,10]]},"reference":[{"key":"4057_CR1","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.livsci.2019.04.007","volume":"224","author":"S Ahmad","year":"2019","unstructured":"Ahmad S, Ali S, Abbas A, Abbas RZ, Zubair M, Raza MA, Bano N, Shafi MU, Badar SN, Hussain K, Rizwan M, Ahmad T (2019) Effects of dietary supplementation of linseed oil (Omega-3) on quality parameters of Nili Ravi bull Spermatozoa. 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