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It can infect mammals and has a global geographical distribution, but data on its occurrence in Egyptian dromedaries and the associated ticks are limited. Therefore, this study aims to detect <jats:italic>C. burnetii<\/jats:italic> in the blood of infested camels and associated ticks collected from Egypt by using molecular techniques and to examine the possibility of coinfections with <jats:italic>C. burnetii.<\/jats:italic> A total of 133 blood samples and 1260 hard ticks infesting these camels were collected from Egyptian slaughterhouses. Nested PCR and sequencing were used based on the IS1111 gene for molecular detection of <jats:italic>C. burnetii.<\/jats:italic> The identification of tick species at the molecular level was performed using the <jats:italic>COX1<\/jats:italic> gene. <jats:italic>C. burnetii<\/jats:italic> was detected in <jats:italic>Hyalomma (H) dromedarii<\/jats:italic>,<jats:italic> H. anatolicum<\/jats:italic>,<jats:italic> H. marginatum<\/jats:italic>,<jats:italic> Amblyomma (Am) lipidium<\/jats:italic>, and <jats:italic>Am. cohaerens<\/jats:italic> with an overall prevalence rate of 1.3% (16\/1260), while in the camel blood samples, it was 15.8% (21\/133). Out of <jats:italic>C. burnetii<\/jats:italic>-positive ticks, there were double infections by <jats:italic>Borrelia<\/jats:italic> species and <jats:italic>C. burnetii<\/jats:italic> in <jats:italic>H. dromedarii<\/jats:italic> and <jats:italic>Am. lipidium<\/jats:italic> and triple infections at one <jats:italic>Am. cohaerens<\/jats:italic> tick (<jats:italic>C. burnetii<\/jats:italic>,<jats:italic> Borrelia<\/jats:italic> spp., and <jats:italic>Babesia microti<\/jats:italic>). In addition, two positive camel blood samples were found to carry <jats:italic>C. burnetii<\/jats:italic> with <jats:italic>Borrelia<\/jats:italic> spp. Our research findings indicate the presence of <jats:italic>Coxiella burnetii<\/jats:italic> among camels and their associated ticks in Egypt and emphasize the potential of having coinfection. To prevent the transmission of this infection to other animal species or humans, appropriate control measures should be implemented.<\/jats:p>","DOI":"10.1007\/s11250-024-04131-7","type":"journal-article","created":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T02:01:50Z","timestamp":1728525710000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular detection of Coxiella burnetii in blood and hard tick-infested Egyptian camels and the possibility of coinfections"],"prefix":"10.1007","volume":"56","author":[{"given":"Radwa","family":"Ashour","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalia","family":"Hamza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"Kadry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha A.","family":"Sabry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,10,10]]},"reference":[{"key":"4131_CR1","doi-asserted-by":"publisher","first-page":"100547","DOI":"10.1016\/j.onehlt.2023.100547","volume":"16","author":"A Abdelbaset","year":"2023","unstructured":"Abdelbaset A, Kwak M, Nonaka N, Nakao R (2023) Human-biting ticks and zoonotic tick-borne pathogens in North Africa: diversity, distribution, and trans-Mediterranean public health challenges. 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