{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T22:49:29Z","timestamp":1773701369846,"version":"3.50.1"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100008258","name":"Hamedan University of Medical Sciences","doi-asserted-by":"publisher","award":["IR02-05-1401000695"],"award-info":[{"award-number":["IR02-05-1401000695"]}],"id":[{"id":"10.13039\/501100008258","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Trop Anim Health Prod"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1007\/s11250-025-04362-2","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T05:13:48Z","timestamp":1741756428000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Role of RB51 vaccine in cow milk contamination of traditional milk sales centers"],"prefix":"10.1007","volume":"57","author":[{"given":"Jamal","family":"Gharekhani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Hemati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Adabi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh Torkaman","family":"Asadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5831-801X","authenticated-orcid":false,"given":"Maryam","family":"Dadar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,3,12]]},"reference":[{"key":"4362_CR1","doi-asserted-by":"publisher","first-page":"1500","DOI":"10.5897\/AJMR11.002","volume":"5","author":"J Akbarmehr","year":"2011","unstructured":"Akbarmehr J (2011) The prevalence of Brucella abortus and Brucella melitensis in local cheese produced in Sarab city, Iran and its public health implication. Afr J Microbiol Res 5:1500\u20131503","journal-title":"Afr J Microbiol Res"},{"key":"4362_CR2","doi-asserted-by":"publisher","first-page":"44420","DOI":"10.1038\/srep44420","volume":"7","author":"S Al Dahouk","year":"2017","unstructured":"Al Dahouk S et al (2017) Brucella spp. of amphibians comprise genomically diverse motile strains competent for replication in macrophages and survival in mammalian hosts. Sci Rep 7:44420","journal-title":"Sci Rep"},{"key":"4362_CR3","first-page":"1107","volume":"78","author":"S Alamian","year":"2023","unstructured":"Alamian S et al (2023) The control program of brucellosis by the Iranian veterinary organization in industrial dairy cattle farms. Arch Razi Inst 78:1107","journal-title":"Arch Razi Inst"},{"key":"4362_CR4","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1111\/j.1751-0813.1975.tb09404.x","volume":"51","author":"G Alton","year":"1975","unstructured":"Alton G et al (1975) The serological diagnosis of bovine brucellosis: an evaluation of the complement fixation, serum agglutination and rose bengal tests. Aust Vet J 51:57\u201363","journal-title":"Aust Vet J"},{"key":"4362_CR5","unstructured":"Alton G, et al (1988) Techniques for the brucellosis laboratory. Institute National de la Recherdie Agrononique, Verger Versailles Cedex: INRA Publications, Paris, p 192"},{"key":"4362_CR6","first-page":"208","volume":"90","author":"J \u00c1lvarez","year":"2011","unstructured":"\u00c1lvarez J et al (2011) Management of an outbreak of brucellosis due to B. melitensis in dairy cattle in Spain. Res J Vet Sci 90:208\u2013211","journal-title":"Res J Vet Sci"},{"key":"4362_CR7","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/j.vetmic.2003.10.024","volume":"98","author":"B Arellano-Reynoso","year":"2004","unstructured":"Arellano-Reynoso B et al (2004) Intracellular trafficking study of a RB51 B. abortus vaccinal strain isolated from cow milk. Vet Microbiol 98:307\u2013312","journal-title":"Vet Microbiol"},{"key":"4362_CR8","doi-asserted-by":"publisher","first-page":"235","DOI":"10.7883\/yoken.JJID.2016.133","volume":"70","author":"FT Asadi","year":"2017","unstructured":"Asadi FT et al (2017) Clinical and diagnostic aspects of brucellosis and antimicrobial susceptibility of Brucella isolates in Hamedan, Iran. Jpn J Infect Dis 70:235\u2013238","journal-title":"Jpn J Infect Dis"},{"key":"4362_CR9","doi-asserted-by":"publisher","first-page":"3435","DOI":"10.1016\/j.vaccine.2004.02.041","volume":"22","author":"DA Ashford","year":"2004","unstructured":"Ashford DA et al (2004) Adverse events in humans associated with accidental exposure to the livestock brucellosis vaccine RB51. Vaccine 22:3435\u20133439","journal-title":"Vaccine"},{"key":"4362_CR10","first-page":"507","volume":"34","author":"S Ashrafganjooyi","year":"2017","unstructured":"Ashrafganjooyi S et al (2017) Isolation and biotyping of Brucella spp. from sheep and goats raw milk in southeastern Iran. Trop Biomed 34:507\u2013511","journal-title":"Trop Biomed"},{"key":"4362_CR11","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1186\/s12917-023-03773-3","volume":"19","author":"JM Blasco","year":"2023","unstructured":"Blasco JM et al (2023) A review of three decades of use of the cattle brucellosis rough vaccine Brucella abortus RB51: myths and facts. BMC Vet Res 19:211","journal-title":"BMC Vet Res"},{"key":"4362_CR12","doi-asserted-by":"publisher","first-page":"2660","DOI":"10.1128\/jcm.32.11.2660-2666.1994","volume":"32","author":"BJ Bricker","year":"1994","unstructured":"Bricker BJ, Halling SM (1994) Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol 32:2660\u20132666","journal-title":"J Clin Microbiol"},{"key":"4362_CR13","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1177\/104063870301500413","volume":"15","author":"BJ Bricker","year":"2003","unstructured":"Bricker BJ et al (2003) Evaluation of the Brucella abortus species\u2013specific polymerase chain reaction assay, an improved version of the Brucella AMOS polymerase chain reaction assay for cattle. J Vet Diagn Invest 15:374\u2013378","journal-title":"J Vet Diagn Invest"},{"key":"4362_CR14","doi-asserted-by":"publisher","first-page":"113","DOI":"10.4102\/jsava.v79i3.256","volume":"79","author":"S Cadmus","year":"2008","unstructured":"Cadmus S, Adesokan H, Stack J (2008) The use of the milk ring test and rose bengal test in brucellosis control and eradication in Nigeria. J S Afr Vet Assoc 79:113\u2013115","journal-title":"J S Afr Vet Assoc"},{"key":"4362_CR15","first-page":"132","volume":"48","author":"S Chalabiani","year":"2019","unstructured":"Chalabiani S et al (2019) The prevalence of Brucellosis in different provinces of Iran during 2013\u20132015, Iran. J Public Health 48:132","journal-title":"J Public Health"},{"key":"4362_CR16","first-page":"51","volume":"76","author":"M Dadar","year":"2021","unstructured":"Dadar M, Alamian S (2021) Identification of main Brucella species implicated in ovine and caprine abortion cases by molecular and classical methods. Arch Razi Inst 76:51","journal-title":"Arch Razi Inst"},{"key":"4362_CR17","first-page":"315","volume":"10","author":"M Dadar","year":"2019","unstructured":"Dadar M et al (2019a) Molecular identification of Brucella species and biovars associated with animal and human infection in Iran. Vet Res Forum 10:315\u2013321","journal-title":"Vet Res Forum"},{"key":"4362_CR18","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.ijfoodmicro.2018.12.009","volume":"292","author":"M Dadar","year":"2019","unstructured":"Dadar M, Shahali Y, Whatmore AM (2019b) Human brucellosis caused by raw dairy products: a review on the occurrence, major risk factors and prevention. Int J Food Microbiol 292:39\u201347","journal-title":"Int J Food Microbiol"},{"key":"4362_CR19","doi-asserted-by":"publisher","first-page":"108775","DOI":"10.1016\/j.foodres.2019.108775","volume":"128","author":"M Dadar","year":"2020","unstructured":"Dadar M et al (2020a) Contamination of milk and dairy products by Brucella species: a global systematic review and meta-analysis. Food Res Int 128:108775","journal-title":"Food Res Int"},{"key":"4362_CR20","doi-asserted-by":"publisher","first-page":"103858","DOI":"10.1016\/j.micpath.2019.103858","volume":"139","author":"M Dadar","year":"2020","unstructured":"Dadar M, Shahali Y, Fakhri Y (2020b) A primary investigation of the relation between the incidence of brucellosis and climatic factors in Iran. Microb Pathog 139:103858","journal-title":"Microb Pathog"},{"key":"4362_CR21","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1080\/01652176.2021.1894501","volume":"41","author":"M Dadar","year":"2021","unstructured":"Dadar M et al (2021) Importance of brucellosis control programs of livestock on the improvement of one health. Vet Q 41:137\u2013151","journal-title":"Vet Q"},{"key":"4362_CR22","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1007\/s11259-023-10228-5","volume":"48","author":"M Dadar","year":"2023","unstructured":"Dadar M et al (2023) Serological, cultural, and molecular analysis of Brucella from Buffalo milk in various regions of Iran. Vet Res Commun 48:427\u2013436","journal-title":"Vet Res Commun"},{"key":"4362_CR23","doi-asserted-by":"publisher","first-page":"107137","DOI":"10.1016\/j.micpath.2024.107137","volume":"198","author":"M Dadar","year":"2025","unstructured":"Dadar M, Alamian S, Zowghi E (2025) Comprehensive study on human brucellosis seroprevalence and Brucella species distribution in Iran (1970\u20132023). Microb Pathog 198:107137","journal-title":"Microb Pathog"},{"key":"4362_CR24","doi-asserted-by":"crossref","unstructured":"Dadar M, et al (2024) Risk factors and control strategies for Brucella spp. and RB51 vaccine shedding in buffalo milk: a cross-sectional study. Acta Trop, 107436","DOI":"10.1016\/j.actatropica.2024.107436"},{"key":"4362_CR25","doi-asserted-by":"publisher","first-page":"e32","DOI":"10.1111\/tbed.14259","volume":"69","author":"MM de Oliveira","year":"2022","unstructured":"de Oliveira MM et al (2022) Efficacy of brucella abortus S19 and RB51 vaccine strains: a systematic review and meta-analysis. Transbound Emerg Dis 69:e32\u2013e51","journal-title":"Transbound Emerg Dis"},{"key":"4362_CR26","doi-asserted-by":"publisher","first-page":"3085","DOI":"10.1128\/JCM.38.8.3085-3086.2000","volume":"38","author":"DR Ewalt","year":"2000","unstructured":"Ewalt DR, Bricker BJ (2000) Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the caccine strains, 19 and RB51. J Clin Microbiol 38:3085\u20133086","journal-title":"J Clin Microbiol"},{"key":"4362_CR27","doi-asserted-by":"publisher","first-page":"103873","DOI":"10.1016\/j.fm.2021.103873","volume":"100","author":"J Gharekhani","year":"2021","unstructured":"Gharekhani J et al (2021) Herd-level contamination of neospora caninum, toxoplasma gondii and Brucella in milk of Iranian dairy farms. Food Microbiol 100:103873","journal-title":"Food Microbiol"},{"key":"4362_CR28","first-page":"349","volume":"21","author":"M Golshani","year":"2017","unstructured":"Golshani M, Buozari S (2017) A review of brucellosis in Iran: epidemiology, risk factors, diagnosis, control, and prevention. Iran Biomed J 21:349","journal-title":"Iran Biomed J"},{"key":"4362_CR29","doi-asserted-by":"crossref","unstructured":"Gruber JF (2020) Notes from the field: Brucella abortus RB51 infections associated with consumption of raw milk from Pennsylvania\u20142017 and 2018. MMWR Morb Mortal Wkly Rep, 69","DOI":"10.15585\/mmwr.mm6915a4"},{"key":"4362_CR30","first-page":"145","volume":"27","author":"S Gul","year":"2007","unstructured":"Gul S, Khan A (2007) Epidemiology and epizootology of brucellosis: a review. Pak Vet J 27:145","journal-title":"Pak Vet J"},{"key":"4362_CR31","first-page":"127","volume":"73","author":"G Gulbaz","year":"2016","unstructured":"Gulbaz G, Kamber U (2016) The detection of Brucella bacteria with PCR and bacteriological method in raw milk and some of the dairy products which are consumed in Kars. Bull UASVM Vet Med 73:127\u2013132","journal-title":"Bull UASVM Vet Med"},{"key":"4362_CR32","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1016\/j.micpath.2017.03.007","volume":"105","author":"I Hashemifar","year":"2017","unstructured":"Hashemifar I et al (2017) Molecular prevalence of putative virulence-associated genes in Brucella melitensis and Brucella abortus isolates from human and livestock specimens in Iran. Microb Pathog 105:334\u2013339","journal-title":"Microb Pathog"},{"key":"4362_CR33","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/S0378-1135(00)00149-8","volume":"73","author":"RL Hornsby","year":"2000","unstructured":"Hornsby RL et al (2000) Selective media for isolation of Brucella abortus strain RB51. Vet Microbiol 73:51\u201360","journal-title":"Vet Microbiol"},{"key":"4362_CR34","first-page":"45","volume":"3","author":"H Hosein","year":"2017","unstructured":"Hosein H et al (2017) Sensitivity and specificity of the commonly used diagnostic procedures of bovine brucellosis. Vet Sci Res Rev 3:45\u201352","journal-title":"Vet Sci Res Rev"},{"key":"4362_CR35","first-page":"54","volume":"8","author":"A Ibrahim","year":"2012","unstructured":"Ibrahim A, AbdelAll A, Amin A (2012) Long-term diagnostic studies for detection of Brucella spp. in milk samples. Glob Vet 8:54\u201361","journal-title":"Glob Vet"},{"key":"4362_CR36","doi-asserted-by":"crossref","unstructured":"Landis JR, Koch GG (1977) The measurement of observer agreement for categorical data, biometrics, 159\u2013174","DOI":"10.2307\/2529310"},{"key":"4362_CR37","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.prevetmed.2009.03.007","volume":"90","author":"M Longo","year":"2009","unstructured":"Longo M et al (2009) Shedding of Brucella abortus rough mutant strain RB51 in milk of water buffalo (Bubalus bubalis). Prev Vet Med 90:113\u2013118","journal-title":"Prev Vet Med"},{"key":"4362_CR38","unstructured":"Lopetegui P (1998) Erradicaci\u00f3n de brucelosis bovina en Chile. Experiencias en el uso de la vacuna cepa RB51. III Foro Nacional de brucelosis, Acapulco, 159\u2013179"},{"key":"4362_CR39","doi-asserted-by":"publisher","first-page":"3484","DOI":"10.1128\/JCM.00837-08","volume":"46","author":"I L\u00f3pez-Go\u00f1i","year":"2008","unstructured":"L\u00f3pez-Go\u00f1i I et al (2008) Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains. J Clin Microbiol 46:3484\u20133487","journal-title":"J Clin Microbiol"},{"key":"4362_CR40","doi-asserted-by":"publisher","first-page":"282","DOI":"10.30699\/ijmm.16.4.282","volume":"16","author":"MM Majzobi","year":"2022","unstructured":"Majzobi MM et al (2022) Brucellosis in humans with the approach of Brucella species contamination in unpasteurized milk and dairy products from Hamadan, Iran. Iran J Microbiol 16:282\u2013287","journal-title":"Iran J Microbiol"},{"key":"4362_CR41","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.actatropica.2016.04.012","volume":"160","author":"KL Miranda","year":"2016","unstructured":"Miranda KL et al (2016) Brucella abortus RB51 in milk of vaccinated adult cattle. Acta Trop 160:58\u201361","journal-title":"Acta Trop"},{"key":"4362_CR42","first-page":"e00438","volume":"61","author":"E Moreno","year":"2023","unstructured":"Moreno E et al (2023) If you\u2019re not confused, you\u2019re not paying attention: ochrobactrum is not Brucella. J Clin Microbiol 61:e00438-e423","journal-title":"J Clin Microbiol"},{"key":"4362_CR43","first-page":"422","volume":"41","author":"Z Naseri","year":"2016","unstructured":"Naseri Z et al (2016) Prevalence of the most common virulence-associated genes among Brucella melitensis isolates from human blood cultures in Hamadan Province, West of Iran. Iran J Med Sci 41:422","journal-title":"Iran J Med Sci"},{"key":"4362_CR44","doi-asserted-by":"publisher","first-page":"185","DOI":"10.15585\/mmwr.mm6807a6","volume":"68","author":"ME Negr\u00f3n","year":"2019","unstructured":"Negr\u00f3n ME et al (2019) Notes from the field: human Brucella abortus RB51 infections caused by consumption of unpasteurized domestic dairy products\u2014United States, 2017\u20132019. Morb Mortal Wkly Rep 68:185","journal-title":"Morb Mortal Wkly Rep"},{"key":"4362_CR45","doi-asserted-by":"crossref","unstructured":"Olsen S (2018) Brucella: a foodborne pathogen. Handbook of foodborne diseases. CRC Press Taylor & Francis Group, 269\u2013276","DOI":"10.1201\/b22030-25"},{"key":"4362_CR46","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1586\/14760584.4.6.915","volume":"4","author":"SC Olsen","year":"2005","unstructured":"Olsen SC, Stoffregen W (2005) Essential role of vaccines in brucellosis control and eradication programs for livestock. Expert Rev Vaccines 4:915\u2013928","journal-title":"Expert Rev Vaccines"},{"key":"4362_CR47","doi-asserted-by":"publisher","first-page":"13","DOI":"10.21608\/avmj.2016.169200","volume":"62","author":"E Ramadan","year":"2016","unstructured":"Ramadan E, Gafer J (2016) Comparative conventional and molecular tools for detection and differentiation of Brucella field and vaccinal strains. Assiut Vet Med J 62:13\u201323","journal-title":"Assiut Vet Med J"},{"key":"4362_CR48","doi-asserted-by":"publisher","first-page":"561","DOI":"10.14202\/vetworld.2015.561-566","volume":"8","author":"DB Sadhu","year":"2015","unstructured":"Sadhu DB et al (2015) Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants. Vet World 8:561","journal-title":"Vet World"},{"key":"4362_CR49","doi-asserted-by":"publisher","first-page":"584624","DOI":"10.1155\/2008\/584624","volume":"2008","author":"M Tittarelli","year":"2008","unstructured":"Tittarelli M et al (2008) Brucella abortus strain RB51 vaccine: immune response after calfhood vaccination and field investigation in Italian cattle population. Clin Dev Immunol 2008:584624","journal-title":"Clin Dev Immunol"},{"key":"4362_CR50","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1023\/A:1006468713614","volume":"24","author":"F Uzal","year":"2000","unstructured":"Uzal F et al (2000) Effect of vaccination with Brucella abortus strain RB51 on heifers and pregnant cattle. Vet Res Commun 24:143\u2013151","journal-title":"Vet Res Commun"},{"key":"4362_CR51","first-page":"625","volume":"55","author":"M Weiner","year":"2011","unstructured":"Weiner M, Iwaniak W, Szulowski K (2011) Comparison of PCR-based AMOS, bruce-ladder and MLVA assays for typing of brucella species. Bull Vet Inst Pulawy 55:625\u2013630","journal-title":"Bull Vet Inst Pulawy"},{"key":"4362_CR52","doi-asserted-by":"publisher","first-page":"3437","DOI":"10.1128\/JCM.37.11.3437-3442.1999","volume":"37","author":"P Yagupsky","year":"1999","unstructured":"Yagupsky P (1999) Detection of brucellae in blood cultures. J Clin Microbiol 37:3437\u20133442","journal-title":"J Clin Microbiol"}],"container-title":["Tropical Animal Health and Production"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-025-04362-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11250-025-04362-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-025-04362-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T13:02:22Z","timestamp":1744376542000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11250-025-04362-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["4362"],"URL":"https:\/\/doi.org\/10.1007\/s11250-025-04362-2","relation":{},"ISSN":["0049-4747","1573-7438"],"issn-type":[{"value":"0049-4747","type":"print"},{"value":"1573-7438","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"11 June 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 February 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 March 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study does not require ethical approval.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"All of the participants included in the study provided informed consent.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"The authors affirm that they have obtained approval for the publication of their research from all participants.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors assert that they have no conflicting interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"114"}}