{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T22:12:36Z","timestamp":1767046356497,"version":"3.48.0"},"reference-count":82,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/50339-5"],"award-info":[{"award-number":["2017\/50339-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2022\/12669-1"],"award-info":[{"award-number":["2022\/12669-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Trop Anim Health Prod"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11250-025-04791-z","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T13:33:14Z","timestamp":1765200794000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fecal metabolome in Nelore bulls fed pre- and post-feedlot diets"],"prefix":"10.1007","volume":"57","author":[{"given":"Matheus Henrique Vargas","family":"de Oliveira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9255-0733","authenticated-orcid":false,"given":"Jessica Moraes","family":"Malheiros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1671-3841","authenticated-orcid":false,"given":"Viviane Andrade","family":"Ligori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9206-4962","authenticated-orcid":false,"given":"Pollyana Ferreira","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6999-5291","authenticated-orcid":false,"given":"Richard Vaquero","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5675-7702","authenticated-orcid":false,"given":"Pablo","family":"Dominguez-Casta\u00f1o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Carolina Carrera","family":"Zerbini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro Henrique de Melo","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2673-7639","authenticated-orcid":false,"given":"Juliana Varchaki","family":"Portes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2717-314X","authenticated-orcid":false,"given":"Andr\u00e9 Michel","family":"de Castilho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9516-9022","authenticated-orcid":false,"given":"Luiz Alberto","family":"Colnago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0049-1546","authenticated-orcid":false,"given":"Josineudson Augusto II de Vasconcel","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,8]]},"reference":[{"key":"4791_CR1","unstructured":"ABIEC (2023). Associa\u00e7\u00e3o brasileira das ind\u00fastrias exportadoras de carne. Beef Report. Available at: http:\/\/Abiec.Com.Br\/Publicacoes\/Beef-Report-2023\/"},{"key":"4791_CR2","doi-asserted-by":"publisher","first-page":"17971","DOI":"10.1038\/s41598-018-36196-4","volume":"8","author":"RTR Almeida","year":"2018","unstructured":"Almeida RTR, Prado RM, Porto C, Santos GT, Huws SA, Pilau EJ (2018) Exploring the rumen fluid metabolome using liquid chromatography-high-resolution mass spectrometry and molecular networking. Sci Rep 8:17971. https:\/\/doi.org\/10.1038\/s41598-018-36196-4","journal-title":"Sci Rep"},{"key":"4791_CR3","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1007\/s11306-010-0227-6","volume":"6","author":"BN Ametaj","year":"2010","unstructured":"Ametaj BN, Zebeli Q, Saleem F, Psychogios N, Lewis MJ, Dunn SM, Xia J, Wishart DS (2010) Metabolomics reveals unhealthy alterations in rumen metabolism with increased proportion of cereal grain in the diet of dairy cows. Metabolomics 6:583\u2013594. https:\/\/doi.org\/10.1007\/s11306-010-0227-6","journal-title":"Metabolomics"},{"key":"4791_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s40104-019-0422-x","volume":"11","author":"BGN Andrade","year":"2020","unstructured":"Andrade BGN, Bressani FA, Cuadrat RRC, Tizioto PC, De Oliveira PSN, Mour\u00e3o GB, Coutinho LL, Reecy JM, Koltes JE, Walsh P, Berndt A, Palhares JCP, Regitano LCA (2020) The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen. J Anim Sci Biotechnol 11:1\u201310. https:\/\/doi.org\/10.1186\/s40104-019-0422-x","journal-title":"J Anim Sci Biotechnol"},{"key":"4791_CR5","doi-asserted-by":"publisher","first-page":"2819","DOI":"10.1128\/AEM.67.6.2819-2822.2001","volume":"67","author":"C Atasoglu","year":"2001","unstructured":"Atasoglu C, James Newbold C, Wallace J, R (2001) Incorporation of [15\u00a0N]Ammonia by the cellulolytic ruminal bacteria fibrobacter succinogenes BL2, Ruminococcus albus SY3, and Ruminococcus flavefaciens 17. Appl Environ Microbiol 67:2819\u20132822. https:\/\/doi.org\/10.1128\/AEM.67.6.2819-2822.2001","journal-title":"Appl Environ Microbiol"},{"key":"4791_CR6","doi-asserted-by":"publisher","first-page":"10756","DOI":"10.1021\/acs.jafc.9b04030","volume":"67","author":"B Bals","year":"2019","unstructured":"Bals B, Teymouri F, Haddad D, Julian WA, Vismeh R, Jones AD, Mor P, Van Soest B, Tyagi A, Vandehaar M, Bringi V (2019) Presence of acetamide in milk and beef from cattle consuming AFEX-Treated crop residues. J Agric Food Chem 67:10756\u201310763. https:\/\/doi.org\/10.1021\/acs.jafc.9b04030","journal-title":"J Agric Food Chem"},{"key":"4791_CR7","doi-asserted-by":"publisher","first-page":"601","DOI":"10.2527\/jas1954.133601x","volume":"13","author":"IJ Belasco","year":"1954","unstructured":"Belasco IJ (1954) New nitrogen feed compounds for ruminants - A laboratory evaluation. J Anim Sci 13:601\u2013610","journal-title":"J Anim Sci"},{"key":"4791_CR8","doi-asserted-by":"publisher","first-page":"5578","DOI":"10.1038\/s41598-020-62485-y","volume":"10","author":"R Bica","year":"2020","unstructured":"Bica R, Palarea-Albaladejo J, Kew W, Uhrin D, Pacheco D, Macrae A, Dewhurst RJ (2020) Nuclear magnetic resonance to detect rumen metabolites associated with enteric methane emissions from beef cattle. Sci Rep 10:5578. https:\/\/doi.org\/10.1038\/s41598-020-62485-y","journal-title":"Sci Rep"},{"key":"4791_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-022-09108-w","volume":"12","author":"R Bica","year":"2022","unstructured":"Bica R, Palarea-Albaladejo J, Lima J, Uhrin D, Miller GA, Bowen JM, Pacheco D, Macrae A, Dewhurst RJ (2022) Methane emissions and rumen metabolite concentrations in cattle fed two different silages. Sci Rep 12:1\u201314. https:\/\/doi.org\/10.1038\/s41598-022-09108-w","journal-title":"Sci Rep"},{"key":"4791_CR10","doi-asserted-by":"publisher","unstructured":"Carro MD, Ungerfeld EM (2015) Utilization of organic acids to manipulate ruminal fermentation and improve ruminant productivity, in: rumen microbiology: From evolution to revolution. pp. 177\u2013197. https:\/\/doi.org\/10.1007\/978-81-322-2401-3","DOI":"10.1007\/978-81-322-2401-3"},{"key":"4791_CR11","doi-asserted-by":"publisher","first-page":"2243","DOI":"10.3390\/ani12172243","volume":"12","author":"D Chen","year":"2022","unstructured":"Chen D, Su M, Zhu H, Zhong G, Wang X, Ma W, Wanapat M, Tan Z (2022) Using untargeted lc-ms metabolomics to identify the association of biomarkers in cattle feces with marbling standard longissimus lumborum. Animals 12:2243. https:\/\/doi.org\/10.3390\/ani12172243","journal-title":"Animals"},{"key":"4791_CR12","doi-asserted-by":"publisher","DOI":"10.1017\/S1466252319000069","author":"P Cholewi\u0144ska","year":"2020","unstructured":"Cholewi\u0144ska P, Czyz K, Nowakowski P, Wyrostek A (2020) The Microbiome of the digestive system of ruminants-a review. Anim Heal Res Rev. https:\/\/doi.org\/10.1017\/S1466252319000069","journal-title":"Anim Heal Res Rev"},{"key":"4791_CR13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s11306-020-1643-x","volume":"16","author":"BA Clemmons","year":"2020","unstructured":"Clemmons BA, Powers JB, Campagna SR, Seay TB, Embree MM, Myer PR (2020) Rumen fluid metabolomics of beef steers differing in feed efficiency. Metabolomics 16:1\u20139. https:\/\/doi.org\/10.1007\/s11306-020-1643-x","journal-title":"Metabolomics"},{"key":"4791_CR14","doi-asserted-by":"publisher","unstructured":"Conteville LC, da Silva JV, Andrade BGN, Cardoso TF, Bruscadin JJ, de Oliveira PSN, Mour\u00e3o GB, Coutinho LL, Palhares JCP, Berndt A, de Medeiros SR, de Regitano LC A (2023) Rumen and fecal microbiomes are related to diet and production traits in Bos indicus beef cattle. Front Microbiol 14. https:\/\/doi.org\/10.3389\/fmicb.2023.1282851","DOI":"10.3389\/fmicb.2023.1282851"},{"key":"4791_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.compag.2018.08.031","volume":"155","author":"UD Dooyum","year":"2018","unstructured":"Dooyum UD, Mallipeddi R, Pamulapati T, Park T, Kim J, Woo S, Ha Y (2018) Interactive livestock feed ration optimization using evolutionary algorithms. Comput Electron Agric 155:1\u201311. https:\/\/doi.org\/10.1016\/j.compag.2018.08.031","journal-title":"Comput Electron Agric"},{"key":"4791_CR16","doi-asserted-by":"publisher","unstructured":"Elolimy A, Alharthi A, Zeineldin M, Parys C, Helmbrecht A, Loor JJ (2019) Supply of methionine during late-pregnancy alters fecal microbiota and metabolome in neonatal dairy calves without changes in daily feed intake. Front Microbiol 10. https:\/\/doi.org\/10.3389\/fmicb.2019.02159","DOI":"10.3389\/fmicb.2019.02159"},{"key":"4791_CR17","doi-asserted-by":"publisher","first-page":"213","DOI":"10.5713\/ab.20.0870","volume":"34","author":"JS Eom","year":"2021","unstructured":"Eom JS, Kim ET, Kim HS, Choi YY, Lee SJ, Lee S, Suk, Kim SH, Lee SS (2021) Metabolomics comparison of serum and urine in dairy cattle using proton nuclear magnetic resonance spectroscopy. Anim Biosci 34:213\u2013222. https:\/\/doi.org\/10.5713\/ab.20.0870","journal-title":"Anim Biosci"},{"key":"4791_CR18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/metabo10060233","volume":"10","author":"A Foroutan","year":"2020","unstructured":"Foroutan A, Fitzsimmons C, Mandal R, Piri-moghadam H, Zheng J, Guo A, Li C, Guan LL, Wishart DS (2020a) The bovine metabolome. Metabolites 10:1\u201326. https:\/\/doi.org\/10.3390\/metabo10060233","journal-title":"Metabolites"},{"key":"4791_CR19","doi-asserted-by":"publisher","first-page":"491","DOI":"10.3390\/metabo10120491","volume":"10","author":"A Foroutan","year":"2020","unstructured":"Foroutan A, Fitzsimmons C, Mandal R, Berjanskii MV, Wishart DS (2020b) Serum metabolite biomarkers for predicting residual feed intake (RFI) of young Angus bulls. Metabolites 10:491. https:\/\/doi.org\/10.3390\/metabo10120491","journal-title":"Metabolites"},{"key":"4791_CR20","doi-asserted-by":"publisher","first-page":"2617","DOI":"10.1038\/s41396-019-0464-2","volume":"13","author":"C Greening","year":"2019","unstructured":"Greening C, Geier R, Wang C, Woods LC, Morales SE, McDonald MJ, Rushton-Green R, Morgan XC, Koike S, Leahy SC, Kelly WJ, Cann I, Attwood GT, Cook GM, Mackie RI (2019) Diverse hydrogen production and consumption pathways influence methane production in ruminants. ISME J 13:2617\u20132632. https:\/\/doi.org\/10.1038\/s41396-019-0464-2","journal-title":"ISME J"},{"key":"4791_CR21","doi-asserted-by":"publisher","first-page":"100295","DOI":"10.1016\/j.animal.2021.100295","volume":"15","author":"PL Greenwood","year":"2021","unstructured":"Greenwood PL (2021) Review: an overview of beef production from pasture and feedlot globally, as demand for beef and the need for sustainable practices increase. Animal 15:100295. https:\/\/doi.org\/10.1016\/j.animal.2021.100295","journal-title":"Animal"},{"key":"4791_CR22","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1111\/j.1365-2672.2007.03327.x","volume":"103","author":"AY Guliye","year":"2007","unstructured":"Guliye AY, Wallace RJ (2007) Effects of aromatic amino acids, phenylacetate and phenylpropionate on fermentation of Xylan by the rumen anaerobic fungi, Neocallimastix frontalis and Piromyces communis. J Appl Microbiol 103:924\u2013929. https:\/\/doi.org\/10.1111\/j.1365-2672.2007.03327.x","journal-title":"J Appl Microbiol"},{"key":"4791_CR23","doi-asserted-by":"publisher","first-page":"682989","DOI":"10.3389\/fmicb.2021.682989","volume":"12","author":"C Huang","year":"2021","unstructured":"Huang C, Ge F, Yao X, Guo X, Bao P, Ma X, Wu X, Chu M, Yan P, Liang C (2021) Microbiome and metabolomics reveal the effects of different feeding systems on the growth and ruminal development of Yaks. Front Microbiol 12:682989. https:\/\/doi.org\/10.3389\/fmicb.2021.682989","journal-title":"Front Microbiol"},{"key":"4791_CR24","doi-asserted-by":"publisher","first-page":"16164","DOI":"10.1038\/s41598-025-00675-2","volume":"15","author":"H Jalal","year":"2025","unstructured":"Jalal H, Sucu E, Cavallini D, Giammarco M, Akram MZ, Karkar B, Gao M, Pompei L, Eduardo J, Prasinou P, Fusano I (2025) Rumen fermentation profile and methane mitigation potential of Mango and avocado byproducts as feed ingredients and supplements. Sci Rep 15:16164. https:\/\/doi.org\/10.1038\/s41598-025-00675-2","journal-title":"Sci Rep"},{"key":"4791_CR25","doi-asserted-by":"publisher","first-page":"4476","DOI":"10.3168\/jds.2023-24147","volume":"107","author":"Q Jiang","year":"2024","unstructured":"Jiang Q, Sherlock DN, Elolimy AA, Yoon I, Loor JJ (2024) Feeding a Saccharomyces cerevisiae fermentation product during a gut barrier challenge in lactating Holstein cows impacts the ruminal microbiota and metabolome. J Dairy Sci 107:4476\u20134494. https:\/\/doi.org\/10.3168\/jds.2023-24147","journal-title":"J Dairy Sci"},{"key":"4791_CR26","doi-asserted-by":"publisher","first-page":"2015","DOI":"10.3168\/jds.S0022-0302(02)74278-1","volume":"85","author":"H Kajikawa","year":"2002","unstructured":"Kajikawa H, Mitsumori M, Ohmomo S (2002) Stimulatory and inhibitory effects of protein amino acids on growth rate and efficiency of mixed ruminal bacteria. J Dairy Sci 85:2015\u20132022. https:\/\/doi.org\/10.3168\/jds.S0022-0302(02)74278-1","journal-title":"J Dairy Sci"},{"key":"4791_CR27","doi-asserted-by":"publisher","first-page":"1200","DOI":"10.3390\/biology12091200","volume":"12","author":"H Kaur","year":"2023","unstructured":"Kaur H, Kaur G, Gupta T, Mittal D, Ali SA (2023) Integrating omics technologies for a comprehensive Understanding of the microbiome and its impact on cattle production. Biology (Basel) 12:1200. https:\/\/doi.org\/10.3390\/biology12091200","journal-title":"Biology (Basel)"},{"key":"4791_CR28","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1007\/s002530050590","volume":"44","author":"WR Kenealy","year":"1995","unstructured":"Kenealy WR, Cao Y, Weimer PJ (1995) Production of Caproic acid by cocultures of ruminal cellulolytic bacteria and clostridium Kluyverigrown on cellulose and ethanol. Appl Microbiol Biotechnol 44:57\u2013513. https:\/\/doi.org\/10.1007\/s002530050590","journal-title":"Appl Microbiol Biotechnol"},{"key":"4791_CR29","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1080\/17450397509423183","volume":"25","author":"C Kijora","year":"1975","unstructured":"Kijora C, G\u00f6rsch R, M\u00fcller J, Bergner H (1975) Untersuchungen zum umsatz von 14 C-15 N-azetamid bei schafen. Arch Tierernahr 25:233\u2013246. https:\/\/doi.org\/10.1080\/17450397509423183","journal-title":"Arch Tierernahr"},{"key":"4791_CR30","doi-asserted-by":"publisher","first-page":"558","DOI":"10.4014\/jmb.1512.12008","volume":"26","author":"SH Kim","year":"2016","unstructured":"Kim SH, Mamuad LL, Kim DW, Kim SK, Lee SS (2016) Fumarate reductase-producing enterococci reduce methane production in rumen fermentation in vitro. J Microbiol Biotechnol 26:558\u2013566. https:\/\/doi.org\/10.4014\/jmb.1512.12008","journal-title":"J Microbiol Biotechnol"},{"key":"4791_CR31","doi-asserted-by":"publisher","unstructured":"Li F, Li C, Chen Y, Liu J, Zhang C, Irving B, Fitzsimmons C, Plastow G, Guan LL (2019) Host genetics influence the rumen microbiota and heritable rumen microbial features associate with feed efficiency in cattle. Microbiome 7. https:\/\/doi.org\/10.1186\/s40168-019-0699-1","DOI":"10.1186\/s40168-019-0699-1"},{"key":"4791_CR32","doi-asserted-by":"publisher","unstructured":"Li Y, Zang Y, Zhao X, Liu L, Qiu Q, Ouyang K, Qu M (2021) Dietary supplementation with creatine pyruvate alters rumen microbiota protein function in heat-stressed beef cattle. Front Microbiol 12. https:\/\/doi.org\/10.3389\/fmicb.2021.715088","DOI":"10.3389\/fmicb.2021.715088"},{"key":"4791_CR33","doi-asserted-by":"publisher","first-page":"902001","DOI":"10.3389\/fvets.2022.902001","volume":"9","author":"Y Li","year":"2022","unstructured":"Li Y, Ma N, Ren L, Wang M, Hu L, Shen Y, Cao Y, Li Q, Li J, Gao Y (2022) Microbiome-Metabolome responses in ruminal content and feces of lactating dairy cows with N-Carbamylglutamate supplementation under heat stress. Front Vet Sci 9:902001. https:\/\/doi.org\/10.3389\/fvets.2022.902001","journal-title":"Front Vet Sci"},{"key":"4791_CR35","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/j.anifeedsci.2017.07.014","volume":"27","author":"Q Liu","year":"2018","unstructured":"Liu Q, Wang C, Li HQ, Guo G, Huo WJ, Pei CX, Zhang SL, Wang H (2018) Effects of dietary protein levels and rumen-protected pantothenate on ruminal fermentation, microbial enzyme activity and bacteria population in blonde d\u2019aquitaine \u00d7 simmental beef steers. Anim Feed Sci Technol 27:202\u2013210. https:\/\/doi.org\/10.1016\/j.anifeedsci.2017.07.014","journal-title":"Anim Feed Sci Technol"},{"key":"4791_CR34","doi-asserted-by":"publisher","first-page":"1116","DOI":"10.3389\/fmicb.2019.01116","volume":"10","author":"C Liu","year":"2019","unstructured":"Liu C, Wu H, Liu S, Chai S, Meng Q, Zhou Z (2019) Dynamic alterations in Yak rumen bacteria community and metabolome characteristics in response to feed type. Front Microbiol 10:1116. https:\/\/doi.org\/10.3389\/fmicb.2019.01116","journal-title":"Front Microbiol"},{"key":"4791_CR36","doi-asserted-by":"publisher","first-page":"323","DOI":"10.5713\/ab.23.0294","volume":"37","author":"RI Mackie","year":"2024","unstructured":"Mackie RI, Kim H, Kim NK, Cann I (2024) Hydrogen production and hydrogen utilization in the rumen: key to mitigating enteric methane production. Anim Biosci 37:323\u2013336. https:\/\/doi.org\/10.5713\/ab.23.0294","journal-title":"Anim Biosci"},{"key":"4791_CR38","doi-asserted-by":"publisher","unstructured":"Malheiros JM, Correia BSB, Ceribeli C, Cardoso DR, Colnago LA, Junior SB, Reecy JM, Mour\u00e3o GB, Coutinho LL, Palhares JCP, Berndt A, de Almeida Regitano LC (2021) Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus). Sci Rep 11. https:\/\/doi.org\/10.1038\/s41598-021-92179-y","DOI":"10.1038\/s41598-021-92179-y"},{"key":"4791_CR37","doi-asserted-by":"publisher","unstructured":"Malheiros JM, Correia BSB, Ceribeli C, Bruscadin JJ, Diniz WJS, Banerjee P, da Silva Vieira D, Cardoso TF, Andrade BGN, Petrini J, Cardoso DR, Colnago LA, Bogusz Junior S, Mour\u00e3o GB, Coutinho LL, Palhares JCP, de Medeiros SR, Berndt A, de Almeida Regitano LC (2023) Ruminal and feces metabolites associated with feed efficiency, water intake and methane emission in Nelore bulls. Sci Rep 13. https:\/\/doi.org\/10.1038\/s41598-023-45330-w","DOI":"10.1038\/s41598-023-45330-w"},{"key":"4791_CR39","doi-asserted-by":"publisher","unstructured":"Mart\u00ednez-\u00c1lvaro M, Auffret MD, Duthie CA, Dewhurst RJ, Cleveland MA, Watson M, Roehe R (2022) Bovine host genome acts on rumen microbiome function linked to methane emissions. Commun Biol 5. https:\/\/doi.org\/10.1038\/s42003-022-03293-0","DOI":"10.1038\/s42003-022-03293-0"},{"key":"4791_CR40","doi-asserted-by":"publisher","unstructured":"Martinez-Fernandez G, Duval S, Kindermann M, Schirra HJ, Denman SE, McSweeney CS (2018) 3-NOP vs. halogenated compound: methane production, ruminal fermentation and microbial community response in forage fed cattle. Front Microbiol 9. https:\/\/doi.org\/10.3389\/fmicb.2018.01582","DOI":"10.3389\/fmicb.2018.01582"},{"key":"4791_CR41","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1079\/bjn19730122","volume":"29","author":"A McAllan","year":"1973","unstructured":"McAllan A, Smith R (1973) Degradation of nucleic acid derivatives by rumen bacteria in vitro. Br J Nutr 29:467\u2013474. https:\/\/doi.org\/10.1079\/bjn19730122","journal-title":"Br J Nutr"},{"key":"4791_CR42","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.smallrumres.2018.10.016","volume":"170","author":"P Mor","year":"2019","unstructured":"Mor P, Bals B, Kumar S, Tyagi N, Reen JK, Tyagi B, Choudhury PK, Tyagi AK (2019) Influence of replacing concentrate mixture with AFEX pellets on rumen fermentation, blood profile and acetamide content in the rumen of crossbred (Alpine \u00d7 Beetle) female goats. Small Rumin Res 170:109\u2013115. https:\/\/doi.org\/10.1016\/j.smallrumres.2018.10.016","journal-title":"Small Rumin Res"},{"key":"4791_CR43","doi-asserted-by":"publisher","first-page":"1024","DOI":"10.1017\/S1751731110000546","volume":"4","author":"DP Morgavi","year":"2010","unstructured":"Morgavi DP, Forano E, Martin C, Newbold CJ (2010) Microbial ecosystem and methanogenesis in ruminants. Animal 4:1024\u20131036. https:\/\/doi.org\/10.1017\/S1751731110000546","journal-title":"Animal"},{"key":"4791_CR44","unstructured":"Murray RK, Granner DK, Mayes PA, Rodwell VW (2003) Harper\u2019s Illustrated Biochemistry. 26th ed. New York (US): lange medical books\/McGraw-Hill; p. 706."},{"key":"4791_CR45","doi-asserted-by":"publisher","unstructured":"Nagaraja TG, Titgemeyer EC, Galyean ML (2016) Nutrition and gastrointestinal microbial ecology. in rumen microbiology: from evolution to revolution. 195\u2013233. https:\/\/doi.org\/10.1007\/978-81-322-2401-3","DOI":"10.1007\/978-81-322-2401-3"},{"key":"4791_CR46","doi-asserted-by":"publisher","DOI":"10.4172\/jcsb.s13-003","author":"T Nanda","year":"2011","unstructured":"Nanda T, Das M, Tripathy K, Ravi Teja R (2011) Metabolomics: the future of systems biology. J Comput Sci Syst Biol S13. https:\/\/doi.org\/10.4172\/jcsb.s13-003","journal-title":"J Comput Sci Syst Biol S13"},{"key":"4791_CR47","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.3168\/jds.S0022-0302(97)76026-0","volume":"80","author":"JE Nocek","year":"1997","unstructured":"Nocek JE (1997) Bovine acidosis: implications on laminitis. J Dairy Sci 80:1005\u20131028. https:\/\/doi.org\/10.3168\/jds.S0022-0302(97)76026-0","journal-title":"J Dairy Sci"},{"key":"4791_CR48","doi-asserted-by":"publisher","first-page":"27","DOI":"10.3390\/metabo8020027","volume":"8","author":"TF O\u2019Callaghan","year":"2018","unstructured":"O\u2019Callaghan TF, V\u00e1zquez-Fresno R, Serra-Cayuela A, Dong E, Mandal R, Hennessy D, McAuliffe S, Dillon P, Wishart DS, Stanton C, Ross RP (2018) Pasture feeding changes the bovine rumen and milk metabolome. Metabolites 8:27. https:\/\/doi.org\/10.3390\/metabo8020027","journal-title":"Metabolites"},{"key":"4791_CR49","doi-asserted-by":"publisher","unstructured":"Owens FN, Basalan M (2016) Ruminal fermentation, in: rumenology. pp. 63\u2013102. https:\/\/doi.org\/10.1007\/978-3-319-30533-2","DOI":"10.1007\/978-3-319-30533-2"},{"key":"4791_CR50","doi-asserted-by":"publisher","unstructured":"Pang K, Chai S, Yang Y, Wang X, Liu S, Wang SX (2022) Dietary forage to concentrate ratios impact on Yak ruminal microbiota and metabolites. Front Microbiol 13. https:\/\/doi.org\/10.3389\/fmicb.2022.964564","DOI":"10.3389\/fmicb.2022.964564"},{"key":"4791_CR51","doi-asserted-by":"publisher","unstructured":"Pang Z, Lu Y, Zhou G, Hui F, Xu L, Viau C, Spigelman AF, MacDonald PE, Wishart DS, Li S, Xia J (2024) MetaboAnalyst 6.0: towards a unified platform for metabolomics data processing, analysis and interpretation. Nucleic Acids Res 1\u20139. https:\/\/doi.org\/10.1093\/nar\/gkae253","DOI":"10.1093\/nar\/gkae253"},{"key":"4791_CR52","doi-asserted-by":"publisher","first-page":"1428","DOI":"10.1038\/ncomms2432","volume":"4","author":"M Poulsen","year":"2013","unstructured":"Poulsen M, Schwab C, Borg Jensen B, Engberg RM, Spang A, Canibe N, H\u00f8jberg O, Milinovich G, Fragner L, Schleper C, Weckwerth W, Lund P, Schramm A, Urich T (2013) Methylotrophic methanogenic thermoplasmata implicated in reduced methane emissions from bovine rumen. Nat Commun 4:1428. https:\/\/doi.org\/10.1038\/ncomms2432","journal-title":"Nat Commun"},{"key":"4791_CR54","first-page":"179","volume":"45","author":"LM Rode","year":"1987","unstructured":"Rode LM, Hespell RB, Russell JB (1987) The effect of pH on ruminal methanogenesis. FEMS Microbiol Ecol 45:179\u2013184","journal-title":"FEMS Microbiol Ecol"},{"key":"4791_CR53","doi-asserted-by":"publisher","first-page":"100789","DOI":"10.1016\/j.animal.2023.100789","volume":"17","author":"P Romero","year":"2023","unstructured":"Romero P, Huang R, Jim\u00e9nez E, Palma-Hidalgo JM, Ungerfeld EM, Popova M, Morgavi DP, Belanche A, Y\u00e1\u00f1ez-Ruiz DR (2023) Evaluating the effect of phenolic compounds as hydrogen acceptors when ruminal methanogenesis is inhibited in vitro \u2013 Part 2. Dairy goats. Animal 17:100789. https:\/\/doi.org\/10.1016\/j.animal.2023.100789","journal-title":"Animal"},{"key":"4791_CR56","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.1126\/science.1058830","volume":"292","author":"JB Russell","year":"2001","unstructured":"Russell JB, Rychlik JL (2001) Factors that alter rumen microbial ecology. Science 292:1119\u20131122. https:\/\/doi.org\/10.1126\/science.1058830","journal-title":"Science"},{"key":"4791_CR55","doi-asserted-by":"crossref","unstructured":"Russell JB, Wallace RJ (1997) Energy-yielding and energy-consuming reactions. In: Hobson, PN, Stewart, CS (eds.), The rumen microbial ecosystem, 2nd edition, Blackie Academic and Professional, London, UK, pp. 246\u2013282","DOI":"10.1007\/978-94-009-1453-7_6"},{"key":"4791_CR57","doi-asserted-by":"publisher","first-page":"6606","DOI":"10.3168\/jds.2012-5403","volume":"95","author":"F Saleem","year":"2012","unstructured":"Saleem F, Ametaj BN, Bouatra S, Mandal R, Zebeli Q, Dunn SM, Wishart DS (2012) A metabolomics approach to uncover the effects of grain diets on rumen health in dairy cows. J Dairy Sci 95:6606\u20136623. https:\/\/doi.org\/10.3168\/jds.2012-5403","journal-title":"J Dairy Sci"},{"key":"4791_CR58","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1007\/s11306-012-0458-9","volume":"9","author":"F Saleem","year":"2013","unstructured":"Saleem F, Bouatra S, Guo AC, Psychogios N, Mandal R, Dunn SM, Ametaj BN, Wishart DS (2013) The bovine ruminal fluid metabolome. Metabolomics 9:360\u2013378. https:\/\/doi.org\/10.1007\/s11306-012-0458-9","journal-title":"Metabolomics"},{"key":"4791_CR59","doi-asserted-by":"publisher","first-page":"114","DOI":"10.3390\/fermentation9020114","volume":"9","author":"RA Sanjorjo","year":"2023","unstructured":"Sanjorjo RA, Tseten T, Kang MK, Kwon M, Kim SW (2023) In pursuit of Understanding the rumen Microbiome. Fermentation 9:114. https:\/\/doi.org\/10.3390\/fermentation9020114","journal-title":"Fermentation"},{"key":"4791_CR60","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1080\/17450397309425595","volume":"23","author":"O Simon","year":"1973","unstructured":"Simon O, M\u00fcnchmeyer R, G\u00f6rsch R, Bergner H (1973) Einflubb von Harnstoff, azetamid und Azetylharnstoff auf die Freien Aminos\u00e4uren des Blutplasmas und die Aminos\u00e4urenzusammensetzung der pansenproteine beim Schaf [Effect of Urea, acetamide and acetyl Urea on the free amino acids of blood plasma and on the. Arch Tierernahr 23:555\u2013566. https:\/\/doi.org\/10.1080\/17450397309425595","journal-title":"Arch Tierernahr"},{"key":"4791_CR61","doi-asserted-by":"publisher","first-page":"1949","DOI":"10.2527\/jas1986.6361949x","volume":"63","author":"LL Slyter","year":"1986","unstructured":"Slyter LL, Chalupa W, Oltjen RR, Weaver JM (1986) Sulfur influences on rumen microorganisms in vitro and in sheep and calves. J Anim Sci 63:1949\u20131959. https:\/\/doi.org\/10.2527\/jas1986.6361949x","journal-title":"J Anim Sci"},{"key":"4791_CR62","doi-asserted-by":"publisher","first-page":"3562","DOI":"10.2527\/1993.7151298x","volume":"70","author":"CJ Sniffen","year":"1992","unstructured":"Sniffen CJ, O\u2019Connor JD, Van Soest PJ, Fox DG, Russell JB (1992) A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. J Anim Sci 70:3562\u20133577. https:\/\/doi.org\/10.2527\/1993.7151298x","journal-title":"J Anim Sci"},{"key":"4791_CR63","doi-asserted-by":"publisher","unstructured":"Taylor BF, Kiene RP (1989) Microbial metabolism of dimethyl sulfide. 202\u2013221. https:\/\/doi.org\/10.1021\/bk-1989-0393.ch013","DOI":"10.1021\/bk-1989-0393.ch013"},{"key":"4791_CR64","doi-asserted-by":"publisher","unstructured":"Ungerfeld EM (2020) Metabolic hydrogen flows in rumen fermentation: principles and possibilities of interventions. Front Microbiol 11. https:\/\/doi.org\/10.3389\/fmicb.2020.00589","DOI":"10.3389\/fmicb.2020.00589"},{"key":"4791_CR65","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1021\/acs.jproteome.9b00655","volume":"19","author":"A Valerio","year":"2020","unstructured":"Valerio A, Casadei L, Giuliani A, Valerio M (2020) Fecal metabolomics as a novel noninvasive method for Short-Term stress monitoring in beef cattle. J Proteome Res 19:845\u2013853. https:\/\/doi.org\/10.1021\/acs.jproteome.9b00655","journal-title":"J Proteome Res"},{"key":"4791_CR66","doi-asserted-by":"publisher","first-page":"e0161362","DOI":"10.1371\/journal.pone.0161362","volume":"11","author":"HJ Van Lingen","year":"2016","unstructured":"Van Lingen HJ, Plugge CM, Fadel JG, Kebreab E, Bannink A, Dijkstra J (2016) Thermodynamic driving force of hydrogen on rumen microbial metabolism: A theoretical investigation. PLoS ONE 11:e0161362. https:\/\/doi.org\/10.1371\/journal.pone.0161362","journal-title":"PLoS ONE"},{"key":"4791_CR68","doi-asserted-by":"publisher","unstructured":"Wallace RJ, Onodera R, Cotta MA (1997) Metabolism of nitrogen-containing compounds. Hobson, PN, Stewart, CS. (eds.), the rumen microbial ecosystem. Springer Netherlands. pp. 283\u2013328. https:\/\/doi.org\/10.1007\/978-94-009-1453-7_7","DOI":"10.1007\/978-94-009-1453-7_7"},{"key":"4791_CR67","doi-asserted-by":"publisher","unstructured":"Wallace RJ, Snelling TJ, McCartney CA, Tapio I, Strozzi F (2017) Application of meta-omics techniques to understand greenhouse gas emissions originating from ruminal metabolism. Genet Sel Evol 49. https:\/\/doi.org\/10.1186\/s12711-017-0285-6","DOI":"10.1186\/s12711-017-0285-6"},{"key":"4791_CR70","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.anifeedsci.2014.04.012","volume":"194","author":"M Wang","year":"2014","unstructured":"Wang M, Sun XZ, Janssen PH, Tang SX, Tan ZL (2014) Responses of methane production and fermentation pathways to the increased dissolved hydrogen concentration generated by eight substrates in in vitro ruminal cultures. Anim Feed Sci Technol 194:1\u201311. https:\/\/doi.org\/10.1016\/j.anifeedsci.2014.04.012","journal-title":"Anim Feed Sci Technol"},{"key":"4791_CR69","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/ani10060957","volume":"10","author":"L Wang","year":"2020","unstructured":"Wang L, Li Y, Zhang Y, Wang L (2020) The effects of different concentrate-to-forage ratio diets on rumen bacterial microbiota and the structures of Holstein cows during the feeding cycle. Animals 10:1\u201317. https:\/\/doi.org\/10.3390\/ani10060957","journal-title":"Animals"},{"key":"4791_CR71","doi-asserted-by":"publisher","unstructured":"Wang Y, Zhao Y, Nan X, Wang, Yue, Cai M, Jiang L, Luo Q, Xiong B (2022a) Rumen-protected glucose supplementation alters fecal microbiota and its metabolic profiles in early lactation dairy cows. Front Microbiol 13. https:\/\/doi.org\/10.3389\/fmicb.2022.1034675","DOI":"10.3389\/fmicb.2022.1034675"},{"key":"4791_CR72","doi-asserted-by":"publisher","unstructured":"Wang X, Xu T, Zhang X, Zhao N, Hu L, Liu H, Zhang Q, Geng Y, Kang S, Xu S (2022b) The response of ruminal microbiota and metabolites to different dietary protein levels in Tibetan sheep on the Qinghai-Tibetan plateau. Front Vet Sci 9. https:\/\/doi.org\/10.3389\/fvets.2022.922817","DOI":"10.3389\/fvets.2022.922817"},{"key":"4791_CR73","doi-asserted-by":"publisher","first-page":"16117","DOI":"10.1096\/fj.201902900RR","volume":"34","author":"Y Wei","year":"2020","unstructured":"Wei Y, Gao J, Kou Y, Liu M, Meng L, Zheng X, Xu S, Liang M, Sun H, Liu Z, Wang Y (2020) The intestinal microbial metabolite desaminotyrosine is an anti-inflammatory molecule that modulates local and systemic immune homeostasis. FASEB J 34:16117\u201316128. https:\/\/doi.org\/10.1096\/fj.201902900RR","journal-title":"FASEB J"},{"key":"4791_CR74","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.biortech.2014.10.054","volume":"175","author":"PJ Weimer","year":"2015","unstructured":"Weimer PJ, Nerdahl M, Brandl DJ (2015) Production of medium-chain volatile fatty acids by mixed ruminal microorganisms is enhanced by ethanol in co-culture with clostridium kluyveri. Bioresour Technol 175:97\u2013101. https:\/\/doi.org\/10.1016\/j.biortech.2014.10.054","journal-title":"Bioresour Technol"},{"key":"4791_CR75","doi-asserted-by":"publisher","unstructured":"Welch CB, Lourenco JM, Krause TR, Seidel DS, Fluharty FL, Pringle TD, Callaway TR (2021) Evaluation of the fecal bacterial communities of angus steers with divergent feed efficiencies across the lifespan from weaning to slaughter. Front Vet Sci 8. https:\/\/doi.org\/10.3389\/fvets.2021.597405","DOI":"10.3389\/fvets.2021.597405"},{"key":"4791_CR76","doi-asserted-by":"publisher","unstructured":"Xu C, Liu W, Sun B, Zhang, Songshan, Zhang S, Yang Y, Lei Y, Chang L, Xie P, Suo H (2021) Multi-Omics analysis reveals a dependent relationship between rumen bacteria and diet of Grass- and Grain-Fed Yaks. Front Microbiol 12. https:\/\/doi.org\/10.3389\/fmicb.2021.642959","DOI":"10.3389\/fmicb.2021.642959"},{"key":"4791_CR77","doi-asserted-by":"publisher","unstructured":"Y\u00e1\u00f1ez-Ruiz DR, Abecia L, Newbold CJ (2015) Manipulating rumen Microbiome and fermentation through interventions during early life: a review. Front Microbiol 6. https:\/\/doi.org\/10.3389\/fmicb.2015.01133","DOI":"10.3389\/fmicb.2015.01133"},{"key":"4791_CR78","doi-asserted-by":"publisher","unstructured":"Yi S, Dai D, Wu H, Chai S, Liu S, Meng Q, Zhou Z (2022) Dietary concentrate-to-forage ratio affects rumen bacterial community composition and metabolome of Yaks. Front Nutr 9. https:\/\/doi.org\/10.3389\/fnut.2022.927206","DOI":"10.3389\/fnut.2022.927206"},{"key":"4791_CR79","doi-asserted-by":"publisher","unstructured":"Zhang R, Zhu W, Jiang L, Mao S (2017a) Comparative metabolome analysis of ruminal changes in holstein dairy cows fed low- or high-concentrate diets. Metabolomics 13. https:\/\/doi.org\/10.1007\/s11306-017-1204-0","DOI":"10.1007\/s11306-017-1204-0"},{"key":"4791_CR80","doi-asserted-by":"publisher","unstructured":"Zhang J, Shi H, Wang Y, Li S, Cao Z, Ji S, He Y, Zhang H (2017b) Effect of dietary forage to concentrate ratios on dynamic profile changes and interactions of ruminal microbiota and metabolites in Holstein heifers. Front Microbiol 8. https:\/\/doi.org\/10.3389\/fmicb.2017.02206","DOI":"10.3389\/fmicb.2017.02206"},{"key":"4791_CR81","doi-asserted-by":"publisher","unstructured":"Zhang Z, Yang L, He Y, Luo X, Zhao S, Jia X (2021) Composition of fecal microbiota in grazing and feedlot. Angus beef cattle. Animals 11 https:\/\/doi.org\/10.3390\/ani11113167.","DOI":"10.3390\/ani11113167"},{"key":"4791_CR82","doi-asserted-by":"publisher","unstructured":"Zhu C, Li C, Wang Y, Laghi L (2019) Characterization of Yak common biofluids metabolome by means of proton nuclear magnetic resonance spectroscopy. Metabolites 9. https:\/\/doi.org\/10.3390\/metabo9030041","DOI":"10.3390\/metabo9030041"}],"container-title":["Tropical Animal Health and Production"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-025-04791-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11250-025-04791-z","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-025-04791-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T22:03:07Z","timestamp":1767045787000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11250-025-04791-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":82,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["4791"],"URL":"https:\/\/doi.org\/10.1007\/s11250-025-04791-z","relation":{},"ISSN":["0049-4747","1573-7438"],"issn-type":[{"type":"print","value":"0049-4747"},{"type":"electronic","value":"1573-7438"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"29 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Consent was obtained from all authors.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"All participants have consented to the submission of the review article to the journal.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to publish"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"528"}}