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Among lung tissues collected from 100 necropsied calves, histopathological analysis revealed fibrinous bronchopneumonia as the most common diagnosis (56\/100).\n                    <jats:italic>P. multocida<\/jats:italic>\n                    was detected immunohistochemically in 55 out of 100 cases (55%) using a specifically produced polyclonal antibody. The highest prevalence of\n                    <jats:italic>P. multocida<\/jats:italic>\n                    was observed in cases of fibrinous bronchopneumonia (82%). At least one of the targeted macrolide resistance genes was detected in 39 (71%) of the 55 IHC-positive cases. The most frequently encountered resistance profiles were the\n                    <jats:italic>msr(E)<\/jats:italic>\n                    \u2009+\u2009\n                    <jats:italic>mph(E)<\/jats:italic>\n                    combination (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200912; 22%) and the triple gene combination\n                    <jats:italic>erm(42)\u2009+\u2009msr(E)\u2009+\u2009mph(E)<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200912; 22%). In conclusion, this study demonstrated that\n                    <jats:italic>P. multocida<\/jats:italic>\n                    plays a key role in fibrinous pneumonia in calves and that these strains harbor high levels and diverse types of macrolide resistance genes, which may contribute to treatment failures. Therefore, routine molecular resistance screening and the development of rational therapeutic strategies are recommended for veterinary practice.\n                  <\/jats:p>","DOI":"10.1007\/s11250-026-05119-1","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T06:03:59Z","timestamp":1780898639000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of Pasteurella multocida and macrolide resistance genes in calf pneumonia: an immunohistochemical and molecular approach"],"prefix":"10.1007","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1297-426X","authenticated-orcid":false,"given":"Mehmet Burak","family":"Ates","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3713-813X","authenticated-orcid":false,"given":"Mustafa","family":"Ortatatli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6184-5408","authenticated-orcid":false,"given":"Funda","family":"Terzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7266-1049","authenticated-orcid":false,"given":"Asli","family":"Balevi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6096-4440","authenticated-orcid":false,"given":"Zafer","family":"Sayin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1595-0557","authenticated-orcid":false,"given":"Ozgur","family":"Ozdemir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0103-5868","authenticated-orcid":false,"given":"Fatih","family":"Hatipoglu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,8]]},"reference":[{"issue":"6","key":"5119_CR1","doi-asserted-by":"publisher","first-page":"1322","DOI":"10.3390\/microorganisms9061322","volume":"9","author":"T Alhamami","year":"2021","unstructured":"Alhamami T, Chowdhury PR, Gomes N, Carr M, Veltman T, Khazandi M, Mollinger J, Deutscher AT, Turni C, Mahdi L et al (2021) First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia. 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