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Oral administration of antioxidants is common. However, the aim of this study was to evaluate whether the intramuscular administration of a vitamin combination, containing \u03b1-tocopherol and \u03b2-carotene, could improve both milk quality and reproductive performance. The animals were divided into two groups: a group treated, at 24\u00a0h postpartum, with a vitamin combination (group D) and a control group (C). For milk quality, concentrations of retinol, \u03b1-tocopherol, fat, protein, lactose, Somatic Cell Count (SCC), non-fat dry matter (NFDM), casein, urea, pH, satured fatty acids (SFA), unsaturated fatty acids (UFA), monounsaturated fatty acids (MUFA) and poliunsaturated fatty acids (PUFA) in milk were assessed at 7 and 30 days post-treatment. Reproductive performance was evaluated using two parameters: pregnancy rate and the calving-conception interval. Retinol concentrations did not differ significantly between groups or across time points. \u03b1-tocopherol levels were significantly higher in group D compared to group C at T30 (0.35\u2009\u00b1\u20090.016 vs. 0.27\u2009\u00b1\u20090.015\u00a0mg\/100\u00a0g;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), whereas in group C exhibited a significant decrease over time. Fat, SFA, UFA, MUFA, and PUFA levels were significantly elevated in group D relative to group C (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), while SCC showed a significant reduction in the treated group than the control one (104.4\u2009\u00b1\u200920.12 vs. 136.6\u2009\u00b1\u200932.24\u2009\u00d7\u200910\u00b3 cells\/mL;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). No significant differences were observed in pregnancy rates; however, the calving-conception interval was significantly shortened in group D (62.23\u2009\u00b1\u20096.17 vs. 72.88\u2009\u00b1\u20095.49 days;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). The obtained results indicate that the parenteral administration of the vitamin combination was associated with changes in selected milk quality parameters and with a shorter calving-conception interval.\n                  <\/jats:p>","DOI":"10.1007\/s11250-026-05137-z","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T06:37:20Z","timestamp":1781246240000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Administration of antioxidants at parturition in Italian Mediterranean lactating buffaloes: findings in the postpartum"],"prefix":"10.1007","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9422-4746","authenticated-orcid":false,"given":"Lorenza","family":"Frattina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1287-478X","authenticated-orcid":false,"given":"Alice","family":"Carbonari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8203-4540","authenticated-orcid":false,"given":"Matteo","family":"Burgio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8620-9320","authenticated-orcid":false,"given":"Giovanni","family":"Bramante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4250-2875","authenticated-orcid":false,"given":"Olga Maria","family":"Andriulo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5496-4855","authenticated-orcid":false,"given":"Edmondo","family":"Ceci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciro","family":"Galdieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonietta","family":"D\u2019Onghia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2792-8848","authenticated-orcid":false,"given":"Vincenzo","family":"Cicirelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2688-0056","authenticated-orcid":false,"given":"Annalisa","family":"Rizzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,12]]},"reference":[{"issue":"10","key":"5137_CR1","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1046\/j.1439-0442.2000.00323.x","volume":"47","author":"A Baldi","year":"2000","unstructured":"Baldi A, Savoini G, Pinotti L, Monfardini E, Cheli F, Orto VD (2000) Effects of Vitamin E and Different Energy Sources on Vitamin E Status, Milk Quality and Reproduction in Transition Cows*. 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