{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:04:07Z","timestamp":1789535047013,"version":"build-2803163510"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Trop Anim Health Prod"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1007\/s11250-026-05331-z","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T04:29:18Z","timestamp":1789532958000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lipid utilization efficiency and metabolic responses in lactating fat-tailed Turki-Qashqaei ewes fed graded levels of cracked safflower"],"prefix":"10.1007","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0426-1332","authenticated-orcid":false,"given":"Shiva","family":"Forouzanfar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7021-6275","authenticated-orcid":false,"given":"Amir","family":"Ahmadpour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4495-6923","authenticated-orcid":false,"given":"Mousa","family":"Zarrin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5582-4168","authenticated-orcid":false,"given":"Mehrdad","family":"Meamar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"issue":"10","key":"5331_CR1","doi-asserted-by":"publisher","first-page":"e77313","DOI":"10.1371\/journal.pone.0077313","volume":"8","author":"SP Alves","year":"2013","unstructured":"Alves SP, Bessa RJB, Quaresma MAG, Kilminster T, Scanlon T, Oldham C, Milton J, Greeff J, Almeida AM (2013) Does the fat tailed Damara ovine breed have a distinct lipid metabolism leading to a high concentration of branched chain fatty acids in tissues? PLoS ONE 8(10):e77313","journal-title":"PLoS ONE"},{"key":"5331_CR3","volume-title":"Official methods of analysis","author":"Association of Official Analytical Chemists","year":"2005","unstructured":"Association of Official Analytical Chemists (2005) Official methods of analysis, 18th edn. AOAC, Gaithersburg, MD","edition":"18"},{"issue":"1","key":"5331_CR4","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1146\/annurev.nutr.23.011702.073408","volume":"23","author":"DE Bauman","year":"2003","unstructured":"Bauman DE, Griinari JM (2003) Nutritional regulation of milk fat synthesis. Annu Rev Nutr 23(1):203\u2013227","journal-title":"Annu Rev Nutr"},{"issue":"2","key":"5331_CR6","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1016\/j.domaniend.2007.02.002","volume":"34","author":"BJ Bradford","year":"2008","unstructured":"Bradford BJ, Allen MS (2008) Negative energy balance increases periprandial ghrelin and growth hormone concentrations in lactating dairy cows. Domest Anim Endocrinol 34(2):196\u2013203","journal-title":"Domest Anim Endocrinol"},{"issue":"1","key":"5331_CR7","doi-asserted-by":"publisher","first-page":"64","DOI":"10.3168\/jds.S0022-0302(80)82888-8","volume":"63","author":"GA Broderick","year":"1980","unstructured":"Broderick GA, Kang JH (1980) Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci 63(1):64\u201375","journal-title":"J Dairy Sci"},{"issue":"1\u20132","key":"5331_CR8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.anifeedsci.2012.02.009","volume":"174","author":"A Buccioni","year":"2012","unstructured":"Buccioni A, Decandia M, Minieri S, Molle G, Cabiddu A (2012) Lipid metabolism in the rumen: new insights on lipolysis and biohydrogenation with an emphasis on the role of endogenous plant factors. Anim Feed Sci Technol 174(1\u20132):1\u201325","journal-title":"Anim Feed Sci Technol"},{"key":"5331_CR9","doi-asserted-by":"publisher","DOI":"10.1079\/9781845933487.0000","volume-title":"Dairy goats feeding and nutrition","author":"A Cannas","year":"2008","unstructured":"Cannas A, Pulina G (2008) Dairy goats feeding and nutrition. CABI Publishing, Wallingford, UK"},{"issue":"1","key":"5331_CR2","doi-asserted-by":"publisher","first-page":"11857","DOI":"10.1038\/s41598-019-48294-y","volume":"9","author":"D Carre\u00f1o","year":"2019","unstructured":"Carre\u00f1o D, Toral PG, Pinloche E, Belenguer A, Y\u00e1\u00f1ez-Ruiz DR, Herv\u00e1s G, McEwan NR, Newbold CJ, Frutos P (2019) Rumen bacterial community responses to DPA, EPA and DHA in cattle and sheep: A comparative in vitro study. Sci rep 9(1):11857","journal-title":"Sci rep"},{"issue":"1","key":"5331_CR11","doi-asserted-by":"publisher","first-page":"29","DOI":"10.5713\/ajas.2008.70035","volume":"21","author":"MB Dolatpanah","year":"2008","unstructured":"Dolatpanah MB, Towhidi A, Farshad A, Rashidi A, Rezayazdi A (2008) Effects of dietary fish oil on semen quality of goats. Asian-Australas J Anim Sci 21(1):29\u201334","journal-title":"Asian-Australas J Anim Sci"},{"key":"5331_CR5","doi-asserted-by":"publisher","first-page":"p106838","DOI":"10.1016\/j.smallrumres.2022.106838","volume":"217","author":"RB Ettoumia","year":"2022","unstructured":"Ettoumia RB, Vernet J, Ortigues-Marty I, Kraiem K, Majdoub-Mathlouthi L (2022) In fat-tailed sheep, variation of energy intake affected growth performances, carcass muscle and tail fat, without modifying carcass fat: A meta-analysis. Small Rumin Res 217:p106838","journal-title":"Small Rumin Res"},{"issue":"1","key":"5331_CR12","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","volume":"226","author":"J Folch","year":"1957","unstructured":"Folch J, Lees M, Stanley S, G.H (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226(1):497\u2013509","journal-title":"J Biol Chem"},{"key":"5331_CR13","doi-asserted-by":"publisher","first-page":"107858","DOI":"10.1016\/j.smallrumres.2026.107858","volume":"263","author":"S Forouzanfar","year":"2026","unstructured":"Forouzanfar S, Ahmadpour A, Zarrin M, Meamar M (2026) Effects of increasing levels of cracked safflower (Carthamus tinctorius) seeds on milk yield and fatty acid profile, and on rumen fermentation in fat-tailed lactating ewes. Small Rumin Res 263:107858","journal-title":"Small Rumin Res"},{"issue":"3","key":"5331_CR14","first-page":"415","volume":"132","author":"IA Forsyth","year":"1992","unstructured":"Forsyth IA, Gabai G, Morgan G (1992) Effect of growth hormone treatment on the distribution of insulin-like growth factor-I between plasma and lymph of lactating sheep. J Endocrinol 132(3):415\u2013422","journal-title":"J Endocrinol"},{"key":"5331_CR15","doi-asserted-by":"publisher","first-page":"431942","DOI":"10.3389\/fphys.2019.00413","volume":"10","author":"KD Galsgaard","year":"2019","unstructured":"Galsgaard KD, Pedersen J, Knop FK, Holst JJ, Albrechtsen W, N.J (2019) Glucagon receptor signaling and lipid metabolism. Front Physiol 10:431942","journal-title":"Front Physiol"},{"key":"5331_CR16","doi-asserted-by":"publisher","first-page":"1581914","DOI":"10.3389\/fgene.2025.1581914","volume":"16","author":"L Gao","year":"2025","unstructured":"Gao L, Zhang Y, Zhang B, Peng W, Liu Y, Zhang Z, Wang J, Wan P, Yang H, Zhao Z (2025) Genome-wide identification of selection signals in fat-tailed and thin-tailed sheep populations. Front Genet 16:1581914","journal-title":"Front Genet"},{"key":"5331_CR42","doi-asserted-by":"publisher","unstructured":"Harfoot CG, Hazelwood GP (1997) Lipid metabolism in the rumen. In: Hobson PN, Stewart CS (eds) The rumen microbial ecosystem, 2nd edn. Chapman & Hall, London, pp. 382\u2013426. https:\/\/doi.org\/10.1007\/978-94-009-1453-7","DOI":"10.1007\/978-94-009-1453-7"},{"issue":"5","key":"5331_CR17","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/j.physbeh.2010.03.019","volume":"100","author":"KM Heppner","year":"2010","unstructured":"Heppner KM, Habegger KM, Day J, Pfluger PT, Perez-Tilve D, Ward B, Gelfanov V, Woods SC, DiMarchi R, Tsch\u00f6p M (2010) Glucagon regulation of energy metabolism. Physiol Behav 100(5):545\u2013548","journal-title":"Physiol Behav"},{"key":"5331_CR18","volume-title":"Animal and vegetable fats and oils \u2014 gas chromatography of fatty acid methyl esters \u2014 Part 4: guidelines for the determination of fatty acid methyl esters by capillary gas chromatography (ISO Standard No. 12966-4)","author":"International Organization for Standardization","year":"2015","unstructured":"International Organization for Standardization (2015) Animal and vegetable fats and oils \u2014 gas chromatography of fatty acid methyl esters \u2014 Part 4: guidelines for the determination of fatty acid methyl esters by capillary gas chromatography (ISO Standard No. 12966-4). ISO, Geneva, Switzerland"},{"issue":"2","key":"5331_CR19","doi-asserted-by":"publisher","first-page":"397","DOI":"10.2527\/jas.2007-0588","volume":"86","author":"TC Jenkins","year":"2008","unstructured":"Jenkins TC, Wallace RJ, Moate PJ, Mosley EE (2008) Board-invited review: recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. J Anim Sci 86(2):397\u2013412","journal-title":"J Anim Sci"},{"issue":"1","key":"5331_CR21","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1186\/s12864-018-4747-1","volume":"19","author":"B Li","year":"2018","unstructured":"Li B, Qiao L, An L, Wang W, Liu J, Ren Y, Pan Y, Jing J, Liu W (2018) Transcriptome analysis of adipose tissues from two fat-tailed sheep breeds reveals key genes involved in fat deposition. BMC Genomics 19(1):338","journal-title":"BMC Genomics"},{"issue":"5","key":"5331_CR20","doi-asserted-by":"publisher","first-page":"6054","DOI":"10.1002\/jcp.27258","volume":"234","author":"J Li","year":"2019","unstructured":"Li J, Li Y, Zhang Y, Liu X, Gao Z, Zhang J, Han X, Sun S (2019) Glucagon attenuates lipid accumulation in cow hepatocytes through AMPK signaling pathway activation. J Cell Physiol 234(5):6054\u20136066","journal-title":"J Cell Physiol"},{"issue":"7","key":"5331_CR22","doi-asserted-by":"publisher","first-page":"1008","DOI":"10.1017\/S175173111000042X","volume":"4","author":"M Louren\u00e7o","year":"2010","unstructured":"Louren\u00e7o M, Ramos-Morales E, Wallace RJ (2010) The role of microbes in rumen lipolysis and biohydrogenation and their manipulation. Animal 4(7):1008\u20131023","journal-title":"Animal"},{"issue":"12","key":"5331_CR23","doi-asserted-by":"publisher","first-page":"2354","DOI":"10.3390\/ani10122354","volume":"10","author":"R Lunsin","year":"2020","unstructured":"Lunsin R, Duangjinda M, Boonsanit D, Dumkhum W (2020) Production performance, nutrient digestibility, and milk composition of dairy ewes supplemented with crushed sunflower seeds and sunflower seed silage in corn silage-based diets. Animals 10(12):2354","journal-title":"Animals"},{"issue":"20","key":"5331_CR24","doi-asserted-by":"publisher","first-page":"3046","DOI":"10.3390\/ani15203046","volume":"15","author":"L Ma","year":"2025","unstructured":"Ma L, Li M, Zhang Y, Sun Y, Zhao X, Zhao Y, Liu J, Zhang X (2025a) Whole-genome resequencing identifies candidate genes for tail fat deposition in sheep. Animals 15(20):3046","journal-title":"Animals"},{"key":"5331_CR25","doi-asserted-by":"publisher","first-page":"116226","DOI":"10.1016\/j.anifeedsci.2025.116226","volume":"320","author":"Z Ma","year":"2025","unstructured":"Ma Z, Li F, Meng Y, Wang L, Guo T, Zhang Z, Weng X, Wang X, Zhang X, Tian D (2025b) Different rumen biohydrogenation extents in lambs: assessing fatty acid composition, growth performance, carcass characteristics, and digestibility. Anim Feed Sci Technol 320:116226","journal-title":"Anim Feed Sci Technol"},{"issue":"6","key":"5331_CR26","doi-asserted-by":"publisher","first-page":"e70644","DOI":"10.1002\/vms3.70644","volume":"11","author":"A Mousaie","year":"2025","unstructured":"Mousaie A, Ghaedifard MR, Azizpour R, Sadeghi AA (2025) Energy intake during the transition period triggers substantial impacts on body weight, milk production efficiency, milk composition and blood biochemical constituents in fat-tailed ewes. Vet Med Sci 11(6):e70644","journal-title":"Vet Med Sci"},{"issue":"3","key":"5331_CR27","first-page":"1533","volume":"96","author":"RA Nafikov","year":"2013","unstructured":"Nafikov RA, Ametaj BN, Bobe G, Koehler KJ, Young JW, Beitz DC (2013) Prevention of fatty liver in transition dairy cows by subcutaneous injections of glucagon. J Dairy Sci 96(3):1533\u20131545","journal-title":"J Dairy Sci"},{"key":"5331_CR28","volume-title":"Nutrient requirements of small ruminants: sheep, goats, cervids, and new world camelids","author":"National Research Council","year":"2007","unstructured":"National Research Council (2007) Nutrient requirements of small ruminants: sheep, goats, cervids, and new world camelids. National Academies, Washington, DC"},{"issue":"4","key":"5331_CR29","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1590\/S1516-35982014000400007","volume":"43","author":"MN Oguz","year":"2014","unstructured":"Oguz MN, Oguz FK, Buyukoglu TI (2014) Effect of different concentrations of dietary safflower seed on milk yield and some rumen and blood parameters at the end stage of lactation in dairy cows. Rev Bras Zootec 43(4):207\u2013211","journal-title":"Rev Bras Zootec"},{"issue":"12","key":"5331_CR30","doi-asserted-by":"publisher","first-page":"10061","DOI":"10.3168\/jds.2017-12924","volume":"100","author":"DL Palmquist","year":"2017","unstructured":"Palmquist DL, Jenkins TC (2017) A 100-year review: fat feeding of dairy cows. J Dairy Sci 100(12):10061\u201310077","journal-title":"J Dairy Sci"},{"issue":"1","key":"5331_CR31","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.smallrumres.2009.10.014","volume":"89","author":"MC Scat\u00e0","year":"2010","unstructured":"Scat\u00e0 MC, Catillo G, Annicchiarico G, De Matteis G, Napolitano F, Signorelli F, Moioli B (2010) Investigation on lactation persistency and IGF-I gene polymorphisms in dairy sheep. Small Rumin Res 89(1):7\u201311","journal-title":"Small Rumin Res"},{"issue":"Suppl 1","key":"5331_CR32","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1017\/S1751731112001681","volume":"7","author":"KJ Shingfield","year":"2013","unstructured":"Shingfield KJ, Bonnet M, Scollan ND, Wood JD (2013) Recent developments in altering the fatty acid composition of ruminant-derived foods. Animal 7(Suppl 1):132\u2013162","journal-title":"Animal"},{"key":"5331_CR33","first-page":"212","volume":"7","author":"M Simonov","year":"2021","unstructured":"Simonov M, Vlizlo V, Stybel V, Peleno R, Salata V, Matviishyn T, Khimych MGO (2021) Levels of insulin-like growth factor in bovine, goat, and sheep milk in different lactation periods: the etiological factor of cancer in humans. Int J One Health 7:212\u2013217","journal-title":"Int J One Health"},{"key":"5331_CR34","first-page":"00029","volume":"42","author":"PG Toral","year":"2024","unstructured":"Toral PG, Bernard L, Belenguer \u00c1, Rouille B, Herv\u00e1s G, Chilliard Y, Frutos P (2024) Controlling rumen biohydrogenation: innovation opportunities in ruminant feeding. BIO Web Conf 42:00029","journal-title":"BIO Web Conf"},{"issue":"11","key":"5331_CR35","first-page":"10057","volume":"101","author":"PG Toral","year":"2018","unstructured":"Toral PG, Gervais R, Herv\u00e1s G, Lunsin R, Frutos P (2018) Milk fatty acid profile of dairy goats fed diets supplemented with soybean oil or linseed oil. J Dairy Sci 101(11):10057\u201310069","journal-title":"J Dairy Sci"},{"key":"5331_CR43","doi-asserted-by":"publisher","unstructured":"Toral PG, Herv\u00e1s G, Fern\u00e1ndez-D\u00edez C, Belenguer A, Frutos P (2021) Rumen biohydrogenation and milk fatty acid profile in dairy ewes divergent for feed efficiency. J Dairy Sci 104(5):5569\u20135582. https:\/\/doi.org\/10.3168\/jds.2020-19061","DOI":"10.3168\/jds.2020-19061"},{"key":"5331_CR36","doi-asserted-by":"publisher","first-page":"11857","DOI":"10.1038\/s41598-019-48294-y","volume":"9","author":"PG Toral","year":"2019","unstructured":"Toral PG, Monahan FJ, Herv\u00e1s G, Frutos P, Suzuki T, Shingfield KJ (2019) Rumen bacterial community responses to DPA, EPA and DHA in cattle and sheep: a comparative in vitro study. Sci Rep 9:11857","journal-title":"Sci Rep"},{"issue":"2","key":"5331_CR37","doi-asserted-by":"publisher","first-page":"282","DOI":"10.2527\/jas1977.442282x","volume":"44","author":"J Van Keulen","year":"1977","unstructured":"Van Keulen J, Young BA (1977) Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. J Anim Sci 44(2):282\u2013287","journal-title":"J Anim Sci"},{"issue":"10","key":"5331_CR38","doi-asserted-by":"publisher","first-page":"3583","DOI":"10.3168\/jds.S0022-0302(91)78551-2","volume":"74","author":"PJ Van Soest","year":"1991","unstructured":"Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74(10):3583\u20133597","journal-title":"J Dairy Sci"},{"issue":"1","key":"5331_CR39","doi-asserted-by":"crossref","first-page":"129","DOI":"10.3390\/foods14010129","volume":"14","author":"E Vargas-Bello-P\u00e9rez","year":"2025","unstructured":"Vargas-Bello-P\u00e9rez E, Riveros JL, K\u00f6brich P, \u00c1lvarez-Melo PA, M\u00e1rquez-Mellado M, G\u00f3mez-Cort\u00e9s P (2025) Enhancing milk fat with nutraceutical properties through feeding chemically protected canola meal protein to supplementary grazing dairy cows. Foods 14(1):129","journal-title":"Foods"},{"key":"5331_CR40","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1038\/s42003-023-04523-9","volume":"6","author":"YX Xu","year":"2023","unstructured":"Xu YX, Wang B, Jing JF, Ma R, Luo YF, Qu Y, Gong Y, Chu MJ, Dong S, Pan H, Li Y, Kong Y, Li W, Liu B, Mao YJ, Yang B, Zhao SG (2023) Whole-body adipose tissue multi-omic analyses in sheep reveal molecular mechanisms underlying local adaptation to extreme environments. Commun Biol 6:159","journal-title":"Commun Biol"},{"key":"5331_CR41","doi-asserted-by":"publisher","first-page":"149577","DOI":"10.1016\/j.gene.2025.149577","volume":"962","author":"S Zheng","year":"2025","unstructured":"Zheng S, Chen X, Fang J, Li Y, Xiao X, Zhang X, Zhang L, Cheng Y, Hao L (2025) The role of insulin-like growth factor-1 in lactation. Gene 962:149577","journal-title":"Gene"}],"container-title":["Tropical Animal Health and Production"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-026-05331-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11250-026-05331-z","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-026-05331-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T04:29:20Z","timestamp":1789532960000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11250-026-05331-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,16]]},"references-count":42,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["5331"],"URL":"https:\/\/doi.org\/10.1007\/s11250-026-05331-z","relation":{},"ISSN":["0049-4747","1573-7438"],"issn-type":[{"value":"0049-4747","type":"print"},{"value":"1573-7438","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,16]]},"assertion":[{"value":"8 June 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 August 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 September 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors confirm that the ethical policies of the journal, as noted on the journal\u2019s author guidelines page, have been adhered to and the appropriate ethical review committee approval has been received. All procedures involving animals were approved by the Institutional Animal Care and Use Committee of Yasouj University (Approval No. 4014441004) in compliance with the Iranian Council of Animal Care (1995) guidelines. Animal welfare was maintained under professional veterinary and scientific supervision, with all handling procedures designed to minimize injury and stress.","order":1,"name":"Ethics","label":"Ethics approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors affirm that there are no financial, personal, professional, or intellectual conflicts of interest that could have influenced the outcomes or interpretation of this work. No funding or financial support was received from any entity that could gain or lose from the publication of these results. Furthermore, no authors have any affiliations with or involvement in organizations with a financial or non-financial interest in the subject matter discussed. All authors declare that the research was conducted and the manuscript was prepared with complete scientific objectivity and independence.","order":2,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"536"}}