{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:54:16Z","timestamp":1791269656087,"version":"4.1.0"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","award":["National Agricultural Science Fund"],"award-info":[{"award-number":["National Agricultural Science Fund"]}],"id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Trop Anim Health Prod"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1007\/s11250-026-05378-y","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:34:23Z","timestamp":1791268463000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular and serological detection of Anaplasma marginale indicates widespread exposure among crossbred cattle in Karnal (Haryana), India"],"prefix":"10.1007","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4302-2969","authenticated-orcid":false,"given":"Sonika","family":"Ahlawat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajay","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijay","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neha","family":"Olla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reena","family":"Arora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rekha","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anish","family":"Yadav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"5378_CR1","doi-asserted-by":"publisher","first-page":"147532","DOI":"10.1016\/j.gene.2023.147532","volume":"877","author":"S Ahlawat","year":"2023","unstructured":"Ahlawat S, Choudhary V, Kaur R, Arora R, Sharma R, Chhabra P, Kumar A, Kaur M (2023) Unraveling the genetic mechanisms governing the host response to bovine anaplasmosis. Gene 877:147532. https:\/\/doi.org\/10.1016\/j.gene.2023.147532","journal-title":"Gene"},{"issue":"2","key":"5378_CR2","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/S1995-7645(13)60010-3","volume":"6","author":"A Ashuma, Sharma","year":"2013","unstructured":"Ashuma, Sharma A, Singla LD, Kaur P, Bal MS, Batth BK, Juyal PD (2013) Prevalence and haemato-biochemical profile of Anaplasma marginale infection in dairy animals of Punjab (India). Asian Pac J Trop Med 6(2):139\u2013144. https:\/\/doi.org\/10.1016\/S1995-7645(13)60010-3","journal-title":"Asian Pac J Trop Med"},{"issue":"1","key":"5378_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1865-1682.2010.01173.x","volume":"58","author":"P Aubry","year":"2011","unstructured":"Aubry P, Geale DW (2011) A review of bovine anaplasmosis. Transbound Emerg Dis 58(1):1\u201330. https:\/\/doi.org\/10.1111\/j.1865-1682.2010.01173.x","journal-title":"Transbound Emerg Dis"},{"issue":"2","key":"5378_CR4","doi-asserted-by":"publisher","first-page":"777","DOI":"10.5433\/1679-0359.2013v34n2p777","volume":"34","author":"J Barbosa da Silva","year":"2013","unstructured":"Barbosa da Silva J, da Fonseca AH (2013) Analysis of the risk factors related to the immune humoral anti-Anaplasma marginale in dairy cattle. Semin Ci\u00eanc Agr\u00e1r 34(2):777\u2013784. https:\/\/doi.org\/10.5433\/1679-0359.2013v34n2p777","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"issue":"2","key":"5378_CR5","first-page":"23","volume":"18","author":"V Bhanot","year":"2022","unstructured":"Bhanot V, Jindal N (2022) Molecular detection of anaplasmosis in crossbred cattle. Indian J Vet Sci Biotechnol 18(2):23\u201327","journal-title":"Indian J Vet Sci Biotechnol"},{"key":"5378_CR6","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/j.vetmic.2004.12.026","volume":"106","author":"ST Echaide","year":"2005","unstructured":"Echaide ST, Bono MF, Lugaresi C et al (2005) Detection of antibodies against Anaplasma marginale in milk using recombinant MSP5 ELISA. Vet Microbiol 106:287\u2013292. https:\/\/doi.org\/10.1016\/j.vetmic.2004.12.026","journal-title":"Vet Microbiol"},{"key":"5378_CR7","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1007\/s40011-015-0555-4","volume":"85","author":"G Filia","year":"2015","unstructured":"Filia G, Mahajan V, Bal MS et al (2015) Seroprevalence of babesiosis and anaplasmosis in large ruminants of Punjab, India. Proc Natl Acad Sci India B 85:885\u2013888. https:\/\/doi.org\/10.1007\/s40011-015-0555-4","journal-title":"Proc Natl Acad Sci India B"},{"issue":"2","key":"5378_CR8","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1177\/104063871002200204","volume":"22","author":"GT Fosgate","year":"2010","unstructured":"Fosgate GT, Urdaz-Rodr\u00edguez JH, Dunbar MD, Rae DO, Donovan GA, Melendez P, Dobek GL, Alleman AR (2010) Diagnostic accuracy of methods for detecting Anaplasma marginale infection in lactating dairy cattle of Puerto Rico. J Vet Diagn Invest 22(2):192\u2013199. https:\/\/doi.org\/10.1177\/104063871002200204","journal-title":"J Vet Diagn Invest"},{"issue":"12","key":"5378_CR9","doi-asserted-by":"publisher","first-page":"1881","DOI":"10.1128\/CVI.00257-10","volume":"17","author":"S Han","year":"2010","unstructured":"Han S, Norimine J, Brayton KA, Palmer GH, Scoles GA, Brown WC (2010) Anaplasma marginale infection with persistent high-load bacteremia induces a dysfunctional memory CD4\u2009+\u2009T lymphocyte response but sustained high IgG titers. Clin Vaccine Immunol 17(12):1881\u20131890. https:\/\/doi.org\/10.1128\/CVI.00257-10","journal-title":"Clin Vaccine Immunol"},{"issue":"4","key":"5378_CR10","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1177\/10406387251324180","volume":"37","author":"RA Ierardi","year":"2025","unstructured":"Ierardi RA (2025) A review of bovine anaplasmosis (Anaplasma marginale) with emphasis on epidemiology and diagnostic testing. J Vet Diagn Invest 37(4):517\u2013538. https:\/\/doi.org\/10.1177\/10406387251324180","journal-title":"J Vet Diagn Invest"},{"issue":"9","key":"5378_CR11","doi-asserted-by":"publisher","first-page":"4281","DOI":"10.1080\/10495398.2022.2146589","volume":"34","author":"R Kaur","year":"2023","unstructured":"Kaur R, Ahlawat S, Choudhary V, Kumari A, Kumar A, Kaur M, Vijh RK (2023) Expression profiling of cytokine genes in peripheral blood mononuclear cells from Anaplasma marginale infected and healthy cattle. Anim Biotechnol 34(9):4281\u20134290. https:\/\/doi.org\/10.1080\/10495398.2022.2146589","journal-title":"Anim Biotechnol"},{"issue":"9","key":"5378_CR12","doi-asserted-by":"publisher","first-page":"2225","DOI":"10.1128\/jcm.34.9.2225-2230.1996","volume":"34","author":"D Knowles","year":"1996","unstructured":"Knowles D, Torioni de Echaide S, Palmer G, McGuire T, Stiller D, McElwain T (1996) Antibody against an Anaplasma marginale MSP5 epitope common to tick and erythrocyte stages identifies persistently infected cattle. J Clin Microbiol 34(9):2225\u20132230. https:\/\/doi.org\/10.1128\/jcm.34.9.2225-2230.1996","journal-title":"J Clin Microbiol"},{"issue":"4","key":"5378_CR14","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1093\/jmedent\/29.4.657","volume":"29","author":"KM Kocan","year":"1992","unstructured":"Kocan KM, Goff WL, Stiller D, Claypool PL, Edwards W, Ewing SA, Hair JA, Barron SJ (1992) Persistence of Anaplasma marginale (Rickettsiales: Anaplasmataceae) in male Dermacentor andersoni (Acari: Ixodidae) transferred successively from infected to susceptible calves. J Med Entomol 29(4):657\u2013668. https:\/\/doi.org\/10.1093\/jmedent\/29.4.657","journal-title":"J Med Entomol"},{"key":"5378_CR13","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.vetpar.2009.09.012","volume":"167","author":"KM Kocan","year":"2010","unstructured":"Kocan KM, de la Fuente J, Blouin EF, Coetzee JF, Ewing SA (2010) The natural history of Anaplasma marginale. Vet Parasitol 167:95\u2013107. https:\/\/doi.org\/10.1016\/j.vetpar.2009.09.012","journal-title":"Vet Parasitol"},{"key":"5378_CR15","first-page":"1","volume":"49","author":"P Kumar","year":"2010","unstructured":"Kumar P, Sangwan A (2010) Comparative prevalence of subclinical bovine anaplasmosis under different cattle management systems in Haryana. Haryana Vet 49:1\u20135","journal-title":"Haryana Vet"},{"key":"5378_CR16","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1111\/j.1751-0813.1999.tb11712.x","volume":"77","author":"J Molloy","year":"1999","unstructured":"Molloy J, Bowles P, Knowles D, McElwain T, Bock R, Kingston T, Blight G, Dalgliesh R (1999) Comparison of a competitive inhibition ELISA and the card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centrale in cattle. Aust Vet J 77:245\u2013249. https:\/\/doi.org\/10.1111\/j.1751-0813.1999.tb11712.x","journal-title":"Aust Vet J"},{"key":"5378_CR17","doi-asserted-by":"publisher","first-page":"106499","DOI":"10.1016\/j.actatropica.2022.106499","volume":"232","author":"K Ntesang","year":"2022","unstructured":"Ntesang K, Singla LD, Kaur P, Arora JS, Kashyap N (2022) Molecular epidemiology, phylogenetic analysis and risk assessment of Anaplasma marginale from naturally infected bovines of Punjab (India). Acta Trop 232:106499. https:\/\/doi.org\/10.1016\/j.actatropica.2022.106499","journal-title":"Acta Trop"},{"issue":"8","key":"5378_CR18","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1089\/vbz.2017.2111","volume":"17","author":"N Ochirkhuu","year":"2017","unstructured":"Ochirkhuu N, Konnai S, Odbileg R, Murata S, Ohashi K (2017) Molecular epidemiological survey and genetic characterization of Anaplasma species in Mongolian livestock. Vector-Borne Zoonotic Dis 17(8):539\u2013549","journal-title":"Vector-Borne Zoonotic Dis"},{"key":"5378_CR19","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.rvsc.2019.01.013","volume":"123","author":"K Paramanandham","year":"2019","unstructured":"Paramanandham K, Mohankumar A, Suresh KP, Jacob SS, Roy P (2019) Prevalence of Anaplasma species in India and the world in dairy animals: A systematic review and meta-analysis. Res Vet Sci 123:159\u2013170. https:\/\/doi.org\/10.1016\/j.rvsc.2019.01.013","journal-title":"Res Vet Sci"},{"issue":"1","key":"5378_CR20","doi-asserted-by":"publisher","first-page":"64","DOI":"10.3390\/pathogens9010064","volume":"9","author":"O Parvizi","year":"2020","unstructured":"Parvizi O, El-Adawy H, Melzer F, Roesler U, Neubauer H, Mertens-Scholz K (2020a) Seroprevalence and molecular detection of bovine anaplasmosis in Egypt. Pathogens 9(1):64. https:\/\/doi.org\/10.3390\/pathogens9010064","journal-title":"Pathogens"},{"issue":"3","key":"5378_CR21","doi-asserted-by":"publisher","first-page":"165","DOI":"10.3390\/pathogens9030165","volume":"9","author":"O Parvizi","year":"2020","unstructured":"Parvizi O, El-Adawy H, Roesler U, Neubauer H, Mertens-Scholz K (2020b) Performance analysis of Anaplasma antibody competitive ELISA using ROC curve for screening of anaplasmosis in camel populations in Egypt. Pathogens 9(3):165. https:\/\/doi.org\/10.3390\/pathogens9030165","journal-title":"Pathogens"},{"key":"5378_CR22","first-page":"4","volume":"12","author":"SS Ramabu","year":"2018","unstructured":"Ramabu SS, Kgwatalala PM, Nsoso SJ, Gasebonwe S, Kgosiesele E (2018) Anaplasma infection prevalence in beef and dairy cattle in the south east region of Botswana. Vet Parasitol Reg Stud Rep 12:4\u20138","journal-title":"Vet Parasitol Reg Stud Rep"},{"issue":"8","key":"5378_CR23","doi-asserted-by":"publisher","first-page":"690","DOI":"10.56093\/ijans.v95i8.164401","volume":"95","author":"Y Ravina, Singh","year":"2025","unstructured":"Ravina, Singh Y, Kaur J, Jhamb R, Charaya G (2025) Statistical evaluation of modalities for diagnosis of anaplasmosis in dairy cattle. Indian J Anim Sci 95(8):690\u2013694. https:\/\/doi.org\/10.56093\/ijans.v95i8.164401","journal-title":"Indian J Anim Sci"},{"issue":"3","key":"5378_CR24","doi-asserted-by":"publisher","first-page":"199","DOI":"10.56093\/ijans.v95i3.157107","volume":"95","author":"SK Sao","year":"2025","unstructured":"Sao SK, Bisen S, Baghel KR, Pal S, Prusty S (2025) Molecular detection and predisposing factors influencing Anaplasma marginale prevalence in dairy cattle of Chhattisgarh, India. Indian J Anim Sci 95(3):199\u2013203","journal-title":"Indian J Anim Sci"},{"key":"5378_CR25","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1007\/s12639-020-01312-7","volume":"45","author":"LN Sarangi","year":"2021","unstructured":"Sarangi LN, Rana SK, Prasad A et al (2021) Prevalence of antibodies to Anaplasma in cattle and buffaloes of different organized herds in India. J Parasit Dis 45:359\u2013365. https:\/\/doi.org\/10.1007\/s12639-020-01312-7","journal-title":"J Parasit Dis"},{"key":"5378_CR26","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.rvsc.2022.12.011","volume":"154","author":"CA Scariot","year":"2022","unstructured":"Scariot CA, Scariot J, de Souza Ramos IA, Gon\u00e7alves LR, Calchi AC, Andr\u00e9 MR, Machado RZ, Costa MM, Kreutz LC, Zanella R, Vieira MIB (2022) Bovine anaplasmosis as a risk factor for retained placenta, mastitis, and abomasal displacement in dairy cattle. Res Vet Sci 154:145\u2013150. https:\/\/doi.org\/10.1016\/j.rvsc.2022.12.011","journal-title":"Res Vet Sci"},{"issue":"7","key":"5378_CR27","doi-asserted-by":"publisher","first-page":"2147","DOI":"10.1128\/JCM.00347-13","volume":"51","author":"AM Schotthoefer","year":"2013","unstructured":"Schotthoefer AM, Meece JK, Ivacic LC, Bertz PD, Zhang K, Weiler T, Uphoff TS, Fritsche TR (2013) Comparison of a real-time PCR method with serology and blood smear analysis for diagnosis of human anaplasmosis: importance of infection time course for optimal test utilization. J Clin Microbiol 51(7):2147\u20132153. https:\/\/doi.org\/10.1128\/JCM.00347-13","journal-title":"J Clin Microbiol"},{"issue":"8","key":"5378_CR28","doi-asserted-by":"publisher","first-page":"1702","DOI":"10.14202\/vetworld.2023.1702-1707","volume":"16","author":"T Seerintra","year":"2023","unstructured":"Seerintra T, Saraphol B, Thanchomnang T, Piratae S (2023) Molecular prevalence of Anaplasma spp. in cattle and assessment of associated risk factors in Northeast Thailand. Vet World 16(8):1702\u20131707. https:\/\/doi.org\/10.14202\/vetworld.2023.1702-1707","journal-title":"Vet World"},{"key":"5378_CR29","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1186\/s12917-018-1489-x","volume":"14","author":"II Shabana","year":"2018","unstructured":"Shabana II, Alhadlag NM, Zaraket H (2018) Diagnostic tools of caprine and ovine anaplasmosis: a direct comparative study. BMC Vet Res 14:165. https:\/\/doi.org\/10.1186\/s12917-018-1489-x","journal-title":"BMC Vet Res"},{"key":"5378_CR30","doi-asserted-by":"publisher","first-page":"352519","DOI":"10.1155\/2015\/352519","volume":"2015","author":"A Sharma","year":"2015","unstructured":"Sharma A, Singla LD, Kaur P, Bal MS (2015) PCR and ELISA vis-\u00e0-vis microscopy for detection of bovine anaplasmosis: A study on associated risk of an upcoming problem in North India. Sci World J 2015:352519. https:\/\/doi.org\/10.1155\/2015\/352519","journal-title":"Sci World J"},{"key":"5378_CR31","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1007\/s11250-005-4307-y","volume":"37","author":"ES Swai","year":"2005","unstructured":"Swai ES, Karimuribo ED, Ogden NH, French NP, Fitzpatrick JL, Bryant MJ, Kambarage DM (2005) Seroprevalence estimation and risk factors for Anaplasma marginale on smallholder dairy farms in Tanzania. Trop Anim Health Prod 37:599\u2013610. https:\/\/doi.org\/10.1007\/s11250-005-4307-y","journal-title":"Trop Anim Health Prod"},{"issue":"1","key":"5378_CR32","doi-asserted-by":"publisher","first-page":"4373981","DOI":"10.1155\/2018\/4373981","volume":"2018","author":"TA Zabel","year":"2018","unstructured":"Zabel TA, Agusto FB (2018) Transmission dynamics of bovine anaplasmosis in a cattle herd. Interdiscip Perspect Infect Dis 2018(1):4373981. https:\/\/doi.org\/10.1155\/2018\/4373981","journal-title":"Interdiscip Perspect Infect Dis"}],"container-title":["Tropical Animal Health and Production"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-026-05378-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11250-026-05378-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11250-026-05378-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:34:24Z","timestamp":1791268464000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11250-026-05378-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,6]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["5378"],"URL":"https:\/\/doi.org\/10.1007\/s11250-026-05378-y","relation":{},"ISSN":["0049-4747","1573-7438"],"issn-type":[{"value":"0049-4747","type":"print"},{"value":"1573-7438","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,6]]},"assertion":[{"value":"17 April 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 September 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare they have no potential Conflicts of interest.","order":1,"name":"Ethics","label":"Conflicts of interest","group":{"name":"EthicsHeading","label":"Declaration"}}],"article-number":"573"}}