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Here, we aimed to reveal serpina3n\/serpina3 role in IC\/BPS in vivo and in vitro.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The IC\/BPS model in mice was induced by intraperitoneal injection of 150\u00a0mg\/kg of cyclophosphamide (CYP). HE and toluidine blue staining were used for histology assessment. Serpina3n\/serpina3 expression in the bladder tissues from IC\/BPS patients and mouse models were determined by qPCR, immunohistochemistry and western blotting. XAV-939 treatment was applied to inhibit \u03b2-catenin activation. Serpina3 role in modulating the growth and apoptosis of HBlEpCs, a human primary bladder epithelial cell line, was assessed by CCK-8 and flow cytometry assays.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Serpina3n\/serpina3 expression was decreased in both human and mice bladder tissues with IC\/BPS. Upregulation of serpina3n significantly alleviated CYP-induced bladder injury, with decreased mast cells and pro-inflammatory factor levels, including IL-1\u03b2, IL-6, and TNF-\u03b1, while increased IL-10 level. In addition, serpina3 overexpression inhibited the apoptosis of HBlEpCs, and increased cell growth. In mechanism, we found that serpina3 overexpression promoted the activation of wnt\/\u03b2-catenin signaling. And, the inhibition of wnt\/\u03b2-catenin signaling with XAV-939 abolished serpina3n\/serpina3 role in protecting bladder tissues from CYP-induced cystitis, as well as inhibiting HBlEpC apoptosis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Serpina3n\/serpina3 expression was decreased in IC\/BPS. Overexpression of serpina3n could alleviate CYP-induced IC\/BPS by activating the Wnt\/\u03b2-catenin signal. This study may provide a new therapeutic strategy for IC\/BPS.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s11255-023-03726-7","type":"journal-article","created":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T04:01:42Z","timestamp":1692331302000},"page":"3065-3075","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Serpina3n\/serpina3 alleviates cyclophosphamide-induced interstitial cystitis by activating the Wnt\/\u03b2-catenin signal"],"prefix":"10.1007","volume":"55","author":[{"given":"Weilin","family":"Fang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qixiang","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwei","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhikang","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,8,18]]},"reference":[{"issue":"5\u20136","key":"3726_CR1","doi-asserted-by":"publisher","first-page":"E136","DOI":"10.5489\/cuaj.3786","volume":"10","author":"A Cox","year":"2016","unstructured":"Cox A, Golda N, Nadeau G, Curtis Nickel J, Carr L, Corcos J et al (2016) CUA guideline: diagnosis and treatment of interstitial cystitis\/bladder pain syndrome. 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