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However, the relationship between SIRT6 and peritoneal fibrosis (PF) in peritoneal dialysis (PD) remains unclear. We collected 110 PD patients with a duration of PD for more than 3\u00a0months and studied the influence of PD duration and history of peritonitis on SIRT6 levels in PD effluents (PDEs). We also analyzed the relationship between SIRT6 levels in PDEs and transforming growth factor beta 1 (TGF-\u03b21), IL-6, PD duration, peritoneal function, PD ultrafiltration (UF), and glucose exposure. We extracted human peritoneal mesothelial cells (HPMCs) from PDEs and measured the protein and gene expression levels of SIRT6, E-cadherin, vimentin, and TGF-\u03b21 in these cells. Based on the clinical results, we used human peritoneal mesothelial cells lines (HMrSV5) to observe the changes in SIRT6 levels and mesothelial-to-mesenchymal transition (MMT) after intervention with PD fluid. By overexpressing and knocking down SIRT6 expression, we investigated the effect of SIRT6 expression on E-cadherin, vimentin, and TGF-\u03b21 expression to elucidate the role of SIRT6 in mesothelial-to-epithelial transition in PMCs. Results: (1) With the extension of PD duration, the influence of infection on SIRT6 levels in PDEs increased. Patients with the PD duration of more than 5\u00a0years and a history of peritonitis had the lowest SIRT6 levels. (2) SIRT6 levels in PDEs were negatively correlated with PD duration, total glucose exposure, TGF-\u03b21, IL-6 levels, and the dialysate-to-plasma ratio of creatinine (Cr4hD\/P), but positively correlated with UF. This indicates that SIRT6 has a protective effect on the peritoneum. (3) The short-term group (PD\u2009\u2264\u20091\u00a0year) had higher SIRT6 and E-cadherin gene and protein levels than the mid-term group (1\u00a0year\u2009&lt;\u2009PD\u2009\u2264\u20095\u00a0years) and long-term group (PD\u2009&gt;\u20095\u00a0years) in PMCs, while vimentin and TGF-\u03b21 levels were lower in the mid-term group and long-term group. Patients with a history of peritonitis had lower SIRT6 and E-cadherin levels than those without such a history. (4) After 4.25% PD fluid intervention for HPMCs, longer intervention time resulted in lower SIRT6 levels. (5) Overexpressing SIRT6 can lead to increased E-cadherin expression and decreased vimentin and TGF-\u03b21 expression in HPMCs. Knocking down SIRT6 expression resulted in decreased E-cadherin expression and increased vimentin and TGF-\u03b21 expression in HPMCs. This indicates that SIRT6 expression can inhibit MMT in HPMCs, alleviate PF associated with PD, and have a protective effect on the peritoneum.<\/jats:p>","DOI":"10.1007\/s11255-024-03970-5","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T15:05:26Z","timestamp":1710428726000},"page":"2659-2670","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Expression and significance of SIRT6 in human peritoneal dialysis effluents and peritoneal mesothelial cells"],"prefix":"10.1007","volume":"56","author":[{"given":"Shuai-Shuai","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Qiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu-Qi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Feng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Shuang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong-Shan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,14]]},"reference":[{"key":"3970_CR1","unstructured":"Sachdeva B, Zulfiqar H, Aeddula NR (2023) Peritoneal Dialysis. In: StatPearls. StatPearls Publishing Copyright \u00a9 2023, StatPearls Publishing LLC, Treasure Island (FL) ineligible companies. Disclosure: Hassam Zulfiqar declares no relevant financial relationships with ineligible companies. Disclosure: Narothama Aeddula declares no relevant financial relationships with ineligible companies"},{"issue":"8","key":"3970_CR2","doi-asserted-by":"publisher","first-page":"1705","DOI":"10.1007\/s11255-021-02785-y","volume":"53","author":"A Hayat","year":"2021","unstructured":"Hayat A, Collins J, Saweirs W (2021) Study of early complications associated with peritoneal dialysis catheters: an analysis of the New Zealand peritoneal dialysis registry data. 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