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Details on the isolation method and purity of distinct cell populations is lacking in currently available protocols leading to inconsistent results across laboratories.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>Bovine endometrial tissue from non-pregnant bovine uteri were collected immediately post-mortem and separated using differential size filtering. Isolations (<jats:italic>n<\/jats:italic>\u2009=\u200915) yielded an average of 3.1\u2009\u00d7\u200910<jats:sup>5<\/jats:sup>\u2009\u00b1\u20090.7\u2009\u00d7\u200910<jats:sup>5<\/jats:sup> epithelial cells and 1.88\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\u2009\u00b1\u20095.44\u2009\u00d7\u200910<jats:sup>5<\/jats:sup> stromal fibroblasts per uterine horn. Following expansion in culture, the purity of cell populations was confirmed using morphology and positive staining for cytokeratin and vimentin which identifies epithelial and stromal fibroblast populations, respectively. Using PCR, cDNA from both cell populations was negative for CD45, a marker of immune cells.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>On challenge with a bacterial PAMP (LPS), epithelial and stromal fibroblasts showed a marked increase in the expression of the inflammatory mediators <jats:italic>IL8<\/jats:italic>, <jats:italic>IL6<\/jats:italic>, <jats:italic>S100A8<\/jats:italic> and <jats:italic>S100A9,<\/jats:italic> with both cell populations displaying distinct expression profiles. Here we provide a detailed methodology on the culture of primary bovine endometrial epithelial and stromal cells and demonstrate these cells provide a physiologically relevant model for studies of endometrial inflammation and its regulation.<\/jats:p>\n<\/jats:sec>","DOI":"10.1007\/s11259-020-09770-3","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T06:04:08Z","timestamp":1582265048000},"page":"29-39","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Improved filtration method to isolate pure populations of primary bovine endometrial epithelial and stromal cells for immunological studies"],"prefix":"10.1007","volume":"44","author":[{"given":"Paul","family":"Kelly","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Barry-Reidy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Brewer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3051-8586","authenticated-orcid":false,"given":"Kieran G.","family":"Meade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cliona","family":"O\u2019Farrelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,2,21]]},"reference":[{"key":"9770_CR1","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1111\/j.1600-0897.2012.01106.x","volume":"67","author":"AM Borges","year":"2012","unstructured":"Borges AM, Healey GD, Sheldon IM (2012) Explants of intact endometrium to model bovine innate immunity and inflammation ex vivo. 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