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However, the precise timing of gut closure in today\u2019s precocious pig is not known. We gavaged piglets immediately after birth and at 1-h after birth with Cy5-labeled Ovalbumin (Cy5-Ova) then harvested their small intestine\u2019s 6\u20137\u00a0h later. To assess localization of Cy5-Ova in the small intestinal epithelial cells, we performed immunohistochemistry using a basolateral surface marker and a recycling endosome marker called pIgR, the late endosomal marker Rab7, and the lysosomal marker LAMP-1. Cy5-Ova co-localized with Rab7 and LAMP-1 in the duodenum and jejunum of 0-h old and 1-h old gavaged piglets, but only in the ileum of 0-h gavaged piglets. These data suggest that movement of Cy5-Ova through the late endosomes to the lysosomes was much reduced in the ileum of 1-h gavaged piglets. Cy5-Ova was largely present in epithelial cell digestive and transport vacuoles, but it did not colocalize with pIgR-positive endosomes in 0-h and 1-h gavaged piglets. Differences in macromolecular uptake across the different regions of the small intestine after only 1-h may be due to prior processing of colostral macromolecules, changes in the intestine due to initiation of colonization by microflora and\/or the initiation of gut-closure. Understanding the relationship between the localization of Cy5-Ova and small intestinal permeability may contribute to establishing whether oral vaccination in the newborn can capitalize on the transient permeability before gut closure to promote immune protection.<\/jats:p>","DOI":"10.1007\/s11259-021-09831-1","type":"journal-article","created":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T14:31:23Z","timestamp":1632493883000},"page":"79-91","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Assessment of intestinal macromolecular absorption in young piglets to pave the way to oral vaccination: preliminary results"],"prefix":"10.1007","volume":"46","author":[{"given":"Brodie","family":"Deluco","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1261-0901","authenticated-orcid":false,"given":"Heather L.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,9,24]]},"reference":[{"key":"9831_CR1","doi-asserted-by":"publisher","first-page":"147","DOI":"10.2334\/josnusd.53.147","volume":"53","author":"M Asano","year":"2011","unstructured":"Asano M, Komiyama K (2011) Polymeric immunoglobulin receptor. 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